Liquid oil painting: Free and forced convection in an enclosure with mechanical and thermal forcing

A fluid dynamics video is linked to this article, which have been submitted to the Gallery of Fluid Motion as part of the 65th American Physical Society meeting of the Division of Fluid Dynamics, held in San Diego, California, USA, over 17-20 November 2012. The video serves to visualize flows generated in a rectangular enclosure that are subjected to both mechanical and thermal forcing through a common horizontal boundary. This system exhibits features consistent with either horizontal convection or lid-driven cavity flows depending on the ratio between thermal and mechanical stirring, and three different cases are visualized in the linked videos.Comment: 2 video files attached, 4 pages, 1 figure. This article is submitted accompanying a video submitted to the Gallery of Fluid Motion as part of the 65th Division of Fluid Dynamics meeting of the American Physical Society (17-20 November, San Diego, CA, USA

Compositional strategy of the book of Judges: an inductive, rhetorical study

Under historical critical scholarship, the book of Judges is generally considered a composite work comprising three distinct and essentially unrelated sections. The central section (2:6-16:31), redacted out of traditional source material, is believed to be originally a part of the larger Deuteronomistic History that runs from Deuteronomy to Kings. The prologue (1:1-2:5) and epilogue (17:1-21:25), on the other hand, are seen as independent compositions that are only artificially appended onto the central section at a later stage of the book's redactional history.In the last two decades, there has been an increasing tendency for the book to be read synchronically as an integrated whole. Although synchronic scholars have drawn attention to the presence of thematic links that connect the different sections of the book, they have yet to justify their integrative approach by exploring whether such links are established by design, and if so, whether they imply compositional unity for the book as a whole in its current canonical form. The present thesis thus seeks to remedy this lack.In Chapter 1, the present thesis is placed in its historical context as scholarship on Judges in the past century is critically surveyed. In Chapters 2-4, rhetorical links between the prologue and the epilogue, the epilogue and central section, and the prologue and central sections are respectively examined in detail. As the evidence seems to suggest that such links are established by conscious design, the implication is that at the compositional level, a closer relationship than has been recognised thus far may indeed exist between the three sections.Recognising that any claim of compositional unity for Judges would inevitably have to answer questions regarding apparent discrepancies in viewpoints within the book, in Chapter 5, the issue of kingship, concerning which critical scholars have discerned divergent voices within the book, is explored. Specifically, it is argued that the "king" referred to in the allegedly pro-monarchic refrain cannot be a reference to the Israelite monarchy to come, but is more likely a reference to YHWH's kingship over His people. Such an understanding would therefore eliminate the problem of divergent viewpoints within the bookIn the final chapter, the various observations and conclusions drawn in previous chapters are brought together, and a case is put forth that the person responsible for the selection and arrangement of the material in the central section must have been the very same person who composed the prologue and epilogue of Judges. This means that the current canonical form of Judges may indeed be a unified piece of composition that can justifiably be read as an integrative whole. Moreover, based on the rhetorical concerns discernible through the various links, it is also possible to identify the implied rhetorical agenda of the book as a call for the rerecognition of the kingly authority of YHWH. This would constitute an implied solution to the progressive deterioration witnessed throughout the book, both at the national and leadership level

Non-invasive molecular imaging of inflammatory macrophages in allograft rejection.

BackgroundMacrophages represent a critical cell type in host defense, development and homeostasis. The ability to image non-invasively pro-inflammatory macrophage infiltrate into a transplanted organ may provide an additional tool for the monitoring of the immune response of the recipient against the donor graft. We therefore decided to image in vivo sialoadhesin (Sn, Siglec 1 or CD169) using anti-Sn mAb (SER-4) directly radiolabelled with (99m)Tc pertechnetate.MethodsWe used a heterotopic heart transplantation model where allogeneic or syngeneic heart grafts were transplanted into the abdomen of recipients. In vivo nanosingle-photon emission computed tomography (SPECT/CT) imaging was performed 7 days post transplantation followed by biodistribution and histology.ResultsIn wild-type mice, the majority of (99m)Tc-SER-4 monoclonal antibody cleared from the blood with a half-life of 167 min and was located predominantly on Sn(+) tissues in the spleen, liver and bone marrow. The biodistribution in the transplantation experiments confirmed data derived from the non-invasive SPECT/CT images, with significantly higher levels of (99m)Tc-SER-4 observed in allogeneic grafts (9.4 (±2.7) %ID/g) compared to syngeneic grafts (4.3 (±10.3) %ID/g) (p = 0.0022) or in mice which received allogeneic grafts injected with (99m)Tc-IgG isotype control (5.9 (±0.6) %ID/g) (p = 0.0185). The transplanted heart to blood ratio was also significantly higher in recipients with allogeneic grafts receiving (99m)Tc-SER-4 as compared to recipients with syngeneic grafts (p = 0.000004) or recipients with allogeneic grafts receiving (99m)Tc-IgG isotype (p = 0.000002).ConclusionsHere, we demonstrate that imaging of Sn(+) macrophages in inflammation may provide an important additional and non-invasive tool for the monitoring of the pathophysiology of cellular immunity in a transplant model

Revealing hidden scenes by photon-efficient occlusion-based opportunistic active imaging

The ability to see around corners, i.e., recover details of a hidden scene from its reflections in the surrounding environment, is of considerable interest in a wide range of applications. However, the diffuse nature of light reflected from typical surfaces leads to mixing of spatial information in the collected light, precluding useful scene reconstruction. Here, we employ a computational imaging technique that opportunistically exploits the presence of occluding objects, which obstruct probe-light propagation in the hidden scene, to undo the mixing and greatly improve scene recovery. Importantly, our technique obviates the need for the ultrafast time-of-flight measurements employed by most previous approaches to hidden-scene imaging. Moreover, it does so in a photon-efficient manner based on an accurate forward model and a computational algorithm that, together, respect the physics of three-bounce light propagation and single-photon detection. Using our methodology, we demonstrate reconstruction of hidden-surface reflectivity patterns in a meter-scale environment from non-time-resolved measurements. Ultimately, our technique represents an instance of a rich and promising new imaging modality with important potential implications for imaging science.Comment: Related theory in arXiv:1711.0629

Microorganisms in the Stratosphere (MIST): In-flight Sterilization with UVC Leds

The stratosphere (10 km to 50 km above sea level) is a unique place on Earth for astrobiological studies of microbes in extreme environments due to the combination of harsh conditions (high ultraviolet radiation, low pressure, desiccation, and low temperatures). Microorganisms in the Stratosphere (MIST) will attempt to characterize the diversity of microbes at these altitudes using a balloon collection device on a meteorological weather balloon. A major challenge of such an aerobiology study is the potential for ground contamination that makes it difficult to distinguish between collected microbes and contaminants. One solution is to use germicidal ultraviolet light emitting diodes (UV LEDs) to sterilize the collection strip. To use this solution, an optimal spatial arrangement of the lights had to be determined to ensure the greatest chance of complete sterilization within the 30 to 60 minute time of balloon ascent. A novel, 3D-printed test stand was developed to experimentally determine viable Bacillus pumilus SAFR-032 spore reduction after exposure to ultraviolet radiation at various times, angles, and distances. Taken together, the experimental simulations suggested that the UV LEDs on the MIST flight hardware should be active for at least 15 minutes and mounted within 4 cm of the illuminated surface at any angle to achieve optimal sterilization. These findings will aid in the production of the balloon collection device to ensure pristine stratospheric microbial samples are collected. Flight hardware capable of in-flight self-sterilization will enable future life detection missions to minimize both forward contamination and false positives

Precise B, B_s and B_c meson spectroscopy from full lattice QCD

We give the first accurate results for $B$ and $B_s$ meson masses from lattice QCD including the effect of $u$, $d$ and $s$ sea quarks, and we improve an earlier value for the $B_c$ meson mass. By using the Highly Improved Staggered Quark action for $u/d$, $s$ and $c$ quarks and NRQCD for the $b$ quarks, we are able to achieve an accuracy in the masses of around 10 MeV. Our results are: $m_B$ = 5.291(18) GeV, $m_{B_s}$ = 5.363(11) GeV and $m_{B_c}$ = 6.280(10) GeV. Note that all QCD parameters here are tuned from other calculations, so these are parameter free tests of QCD against experiment. We also give scalar, $B_{s0}^*$, and axial vector, $B_{s1}$, meson masses. We find these to be slightly below threshold for decay to $BK$ and $B^*K$ respectively.Comment: 22 pages, 19 figure

Cooperativity and Frustration in Protein-Mediated Parallel Actin Bundles

We examine the mechanism of bundling of cytoskeletal actin filaments by two representative bundling proteins, fascin and espin. Small-angle X-ray studies show that increased binding from linkers drives a systematic \textit{overtwist} of actin filaments from their native state, which occurs in a linker-dependent fashion. Fascin bundles actin into a continuous spectrum of intermediate twist states, while espin only allows for untwisted actin filaments and fully-overtwisted bundles. Based on a coarse-grained, statistical model of protein binding, we show that the interplay between binding geometry and the intrinsic \textit{flexibility} of linkers mediates cooperative binding in the bundle. We attribute the respective continuous/discontinous bundling mechanisms of fascin/espin to differences in the stiffness of linker bonds themselves.Comment: 5 pages, 3 figures, figure file has been corrected in v