9 research outputs found

    Saturation behavior of superoxide dismutation catalyzed by the iron containing superoxide dismutase of E. coli B

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    SummaryThe iron containing superoxide dismutase from E. coli B is shown to catalyze superoxide dismutation by a mechanism which exhibits saturation kinetics. This behavior is quite different from that observed previously with bovine Zn/Cu- and iron-containing superoxide dismutase from P. leiognathi. Two parameters of catalysis were measured in the pH range 7.2 to 10.4: kcat was found to be independent of pH and Km varied with the function Km = Km(low pH) [1 + exp(pH -- 8.8)]. These results implicate a group in the catalytic mechanism which ionizes with pKa = 8.8.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/24375/1/0000644.pd

    The Toxicology of Molecular Oxygen

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    Superoxide Dismutases and Superoxide Reductases

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