A newly discovered subglacial lake in East Antarctica likely hosts a valuable sedimentary record of ice and climate change

The Princess Elizabeth Land sector of the East Antarctic Ice Sheet is a significant reservoir of grounded ice and is adjacent to regions that experienced great change during Quaternary glacial cycles and Pliocene warm episodes. The existence of an extensive subglacial water system in Princess Elizabeth Land (to date only inferred from satellite imagery) bears the potential to significantly impact the thermal and kinematic conditions of the overlying ice sheet. We confirm the existence of a major subglacial lake, herein referred to as Lake Snow Eagle (LSE), for the first time using recently acquired aerogeophysical data. We systematically investigated LSE’s geological characteristics and bathymetry from two-dimensional geophysical inversion models. The inversion results suggest that LSE is located along a compressional geologic boundary, which provides reference for future characterization of the geologic and tectonic context of this region. We estimate LSE to be ~42 km in length and 370 km2 in area, making it one of the largest subglacial lakes in Antarctica. Additionally, the airborne ice-penetrating radar observations and geophysical inversions reveal a layer of unconsolidated water-saturated sediment around and at the bottom of LSE, which—given the ultralow rates of sedimentation expected in such environments—may archive valuable records of paleoenvironmental changes and the early history of East Antarctic Ice Sheet evolution in Princess Elizabeth Land

Enhanced surgical site infection surveillance following hysterectomy, vascular, and colorectal surgery

Objective.To evaluate the use of inpatient pharmacy and administrative data to detect surgical site infections (SSIs) following hysterectomy and colorectal and vascular surgery.Design.Retrospective cohort study.Setting.Five hospitals affiliated with academic medical centers.Patients.Adults who underwent abdominal or vaginal hysterectomy, colorectal surgery, or vascular surgery procedures between July 1, 2003, and June 30, 2005.Methods.We reviewed the medical records of weighted, random samples drawn from 3,079 abdominal and vaginal hysterectomy, 4,748 colorectal surgery, and 3,332 vascular surgery procedures. We compared routine surveillance with screening of inpatient pharmacy data and diagnosis codes and then performed medical record review to confirm SSI status.Results.Medical records from 823 hysterectomy, 736 colorectal surgery, and 680 vascular surgery procedures were reviewed. SSI rates determined by antimicrobial- and/or diagnosis code-based screening followed by medical record review (enhanced surveillance) were substantially higher than rates determined by routine surveillance (4.3% [95% confidence interval, 3.6%—5.1%] vs 2.7% for hysterectomies, 7.1% [95% confidence interval, 6.7%–8.2%] vs 2.0% for colorectal procedures, and 2.3% [95% confidence interval, 1.9%–2.9%] vs 1.4% for vascular procedures). Enhanced surveillance had substantially higher sensitivity than did routine surveillance to detect SSI (92% vs 59% for hysterectomies, 88% vs 22% for colorectal procedures, and 72% vs 43% for vascular procedures). A review of medical records confirmed SSI for 31% of hysterectomies, 20% of colorectal procedures, and 31% of vascular procedures that met the enhanced screening criteria.Conclusion.Antimicrobial- and diagnosis code-based screening may be a useful method for enhancing and streamlining SSI surveillance for a variety of surgical procedures, including those procedures targeted by the Centers for Medicare and Medicaid Services.</jats:sec

Text Messaging for Disease Monitoring in Childhood Nephrotic Syndrome

© 2019 International Society of Nephrology Introduction: There is limited information on effective disease monitoring for prompt interventions in childhood nephrotic syndrome. We examined the feasibility and effectiveness of a novel text messaging system (SMS) for disease monitoring in a multicenter, prospective study. Methods: A total of 127 patients results, symptoms, and medication adherence were sent to a designated caregiver (n = 116) or adolescent patient (n = 3). Participants responded by texting. Feasibility of SMS was assessed by SMS adoption, retention, and engagement, and concordance between participant-reported results and laboratory/clinician assessments. The number of disease relapses and time-to-remission data captured by SMS were compared with data collected by conventional visits. Results: A total of 119 of 127 (94%) patients agreed to SMS monitoring. Retention rate was 94%, with a median follow-up of 360 days (interquartile range [IQR] 353–362). Overall engagement was high, with a median response rate of 87% (IQR, 68–97). Concordance between SMS-captured home urine protein results and edema status with same-day in-person study visit was excellent (kappa values 0.88 and 0.92, respectively). SMS detected a total of 108 relapse events compared with 41 events captured by scheduled visits. Median time to remission after enrollment was 22 days as captured by SMS versus 50 days as captured by scheduled visits. Conclusion: SMS was well accepted by caregivers and adolescent patients and reliably captured nephrotic syndrome disease activity between clinic visits. Additional studies are needed to explore the impact of SMS on disease outcomes

Apicomplexan Parasites Co-Opt Host Calpains to Facilitate Their Escape from Infected Cells

Apicomplexan parasites, including Plasmodium falciparum and Toxoplasma gondii (the causative agents of malaria and toxoplasmosis, respectively), are responsible for considerable morbidity and mortality worldwide. These pathogenic protozoa replicate within an intracellular vacuole inside of infected host cells, from which they must escape to initiate a new lytic cycle. By integrating cell biological, pharmacological, and genetic approaches, we provide evidence that both Plasmodium and Toxoplasma hijack host cell calpain proteases to facilitate parasite egress. Immunodepletion or inhibition of calpain-1 in hypotonically lysed and resealed erythrocytes prevented the escape of P. falciparum parasites, which was restored by adding purified calpain-1. Similarly, efficient egress of T. gondii from mammalian fibroblasts was blocked by either small interfering RNA– mediated suppression or genetic deletion of calpain activity and could be restored by genetic complementation

Probing T-cell response by sequence-based probabilistic modeling

With the increasing ability to use high-throughput next-generation sequencing to quantify the diversity of the human T cell receptor (TCR) repertoire, the ability to use TCR sequences to infer antigen-specificity could greatly aid potential diagnostics and therapeutics. Here, we use a machine-learning approach known as Restricted Boltzmann Machine to develop a sequence-based inference approach to identify antigen-specific TCRs. Our approach combines probabilistic models of TCR sequences with clone abundance information to extract TCR sequence motifs central to an antigen-specific response. We use this model to identify patient personalized TCR motifs that respond to individual tumor and infectious disease antigens, and to accurately discriminate specific from non-specific responses. Furthermore, the hidden structure of the model results in an interpretable representation space where TCRs responding to the same antigen cluster, correctly discriminating the response of TCR to different viral epitopes. The model can be used to identify condition specific responding TCRs. We focus on the examples of TCRs reactive to candidate neoantigens and selected epitopes in experiments of stimulated TCR clone expansion

Modelled fracture and calving on the Totten Ice Shelf

The Totten Ice Shelf (IS) has a large drainage basin, much of which is grounded below sea level, leaving the glacier vulnerable to retreat through the marine ice sheet instability mechanism. The ice shelf has also been shown to be sensitive to changes in calving rate, as a very small retreat of the calving front from its current position is predicted to cause a change in flow at the grounding line. Therefore understanding the processes behind calving on the Totten IS is key to predicting its future sea level rise contribution. Here we use the Helsinki Discrete Element Model (HiDEM) to show that not all of the fractures visible at the front of the Totten IS are produced locally, but that the across-flow basal crevasses, which are part of the distinctive cross-cutting fracture pattern, are advected into the calving front area from upstream. A separate simulation of the grounding line shows that re-grounding points may be key areas of basal crevasse production, and can produce basal crevasses in both an along- and across-flow orientation. The along-flow basal crevasses at the grounding line may be a possible precursor to basal channels, while we suggest the across-flow grounding-line fractures are the source of the across-flow features observed at the calving front. We use two additional models to simulate the evolution of basal fractures as they advect downstream, demonstrating that both strain and ocean melt have the potential to deform narrow fractures into the broad basal features observed near the calving front. The wide range of factors which influence fracture patterns and calving on this glacier will be a challenge for predicting its future mass loss.</p

Irreversible Capacities of Graphite in Low-Temperature Electrolytes for Lithium-Ion Batteries

Carbonaceous anode materials in lithium-ion rechargeable cells exhibit irreversible capacity, mainly due to reaction of lithium during the formation of passive surface films. The stability and kinetics of lithium intercalation into the carbon anodes are determined by these films. The nature, thickness, and morphology of these films are in turn affected by the electrolyte components, primarily the solvent constituents. In this work, the films formed on graphite anodes in low-temperature electrolytes, i.e., solutions with different mixtures of alkyl carbonates and low-viscosity solvent additives, are examined using electrochemical impedance spectroscopy (EIS) and solid-state ^(7)Li nuclear magnetic resonance techniques. In addition, other ex situ studies such as X-ray diffraction, transmission electron microscopy, and electron energy loss spectroscopy were carried out on the graphite anodes to understand their microstructures

Immune epitope database analysis resource (IEDB-AR)

We present a new release of the immune epitope database analysis resource (IEDB-AR, http://tools.immuneepitope.org), a repository of web-based tools for the prediction and analysis of immune epitopes. New functionalities have been added to most of the previously implemented tools, and a total of eight new tools were added, including two B-cell epitope prediction tools, four T-cell epitope prediction tools and two analysis tools

T-Cell Memory Responses Elicited by Yellow Fever Vaccine are Targeted to Overlapping Epitopes Containing Multiple HLA-I and -II Binding Motifs

The yellow fever vaccines (YF-17D-204 and 17DD) are considered to be among the safest vaccines and the presence of neutralizing antibodies is correlated with protection, although other immune effector mechanisms are known to be involved. T-cell responses are known to play an important role modulating antibody production and the killing of infected cells. However, little is known about the repertoire of T-cell responses elicited by the YF-17DD vaccine in humans. In this report, a library of 653 partially overlapping 15-mer peptides covering the envelope (Env) and nonstructural (NS) proteins 1 to 5 of the vaccine was utilized to perform a comprehensive analysis of the virus-specific CD4+ and CD8+ T-cell responses. The T-cell responses were screened ex-vivo by IFN-γ ELISPOT assays using blood samples from 220 YF-17DD vaccinees collected two months to four years after immunization. Each peptide was tested in 75 to 208 separate individuals of the cohort. The screening identified sixteen immunodominant antigens that elicited activation of circulating memory T-cells in 10% to 33% of the individuals. Biochemical in-vitro binding assays and immunogenetic and immunogenicity studies indicated that each of the sixteen immunogenic 15-mer peptides contained two or more partially overlapping epitopes that could bind with high affinity to molecules of different HLAs. The prevalence of the immunogenicity of a peptide in the cohort was correlated with the diversity of HLA-II alleles that they could bind. These findings suggest that overlapping of HLA binding motifs within a peptide enhances its T-cell immunogenicity and the prevalence of the response in the population. In summary, the results suggests that in addition to factors of the innate immunity, "promiscuous" T-cell antigens might contribute to the high efficacy of the yellow fever vaccines. © 2013 de Melo et al