USING OTOLITH MICROCHEMISTRY TO CLASSIFY YELLOW PERCH AS STOCKED OR NATURALLY PRODUCED

Fisheries managers routinely use stocking to supplement fish populations (Schramm and Piper 1995, Fisher 1996). Stocking eyed-eggs offers substantial cost savings compared to stocking fry and fingerlings (PFBC 2011); however, traditional stocking evaluation using oxytetracycline (OTC) marking of otoliths is ineffective for eyed-eggs of some species (e.g., yellow perch, [Perca fla- vescens]). Thus, there is a need for additional approaches to be able to classify fish as stocked or naturally produced. Fish otoliths are paired calcified structures in the inner ear that permanently deposit trace elements in proportion to water column concentrations (Campana 1999, Campana et al. 2000). Coupled with otolith growth increments (i.e., annuli), elemental accumulation permits retrospective evaluation of environmental history (e.g., natal origins, movement) if water chemistry is spatially heterogeneous and temporally constant (Elsdon et al. 2008). Otolith microchemistry can be used to evaluate stocking contributions (Pracheil et al. 2014) and in the context of eyed-egg stockings, may be useful for classifying fish as stocked or naturally produced. Yellow perch is a popular sport fish species in South Dakota (Gigliotti 2007) and is routinely stocked by fisheries managers to supplement weak year classes (Schoene- beck et al. 2010). The South Dakota Department of Game, Fish and Parks (SDGFP) propagates yellow perch for stocking (e.g., eyed-eggs, fry, fingerlings) and also stocks adult perch through trap and transfer operations (Lott 1991, Fisher 1996). However, the contributions of yellow perch stockings in South Dakota are largely unknown because it is difficult to differentiate stocked fish from resident individuals (Brown and St. Sauver 2002). Our objective was to assess the utility of otolith microchemistry to distinguish hatchery-reared yellow perch stocked at the eyed-egg stage from naturally produced individuals

Size-Dependent Trophic Patterns of Pallid Sturgeon and Shovelnose Sturgeon in a Large River System

This study compared patterns of d15N and d13C enrichment of pallid sturgeon Scaphirhynchus albus and shovelnose sturgeon S. platorynchus in the Missouri River, United States, to infer their trophic position in a large river system. We examined enrichment and energy flow for pallid sturgeon in three segments of the Missouri River (Montana/North Dakota, Nebraska/South Dakota, and Nebraska/Iowa) and made comparisons between species in the two downstream segments (Nebraska/South Dakota and Nebraska/Iowa). Patterns in isotopic composition for pallid sturgeon were consistent with gut content analyses indicating an ontogenetic diet shift from invertebrates to fish prey at sizes of .500-mm fork length (FL) in all three segments of the Missouri River. Isotopic patterns revealed shovelnose sturgeon did not experience an ontogenetic shift in diet and used similar prey resources as small (,500-mm FL) pallid sturgeon in the two downstream segments. We found stable isotope analysis to be an effective tool for evaluating the trophic position of sturgeons within a large river food web

Yellow perch population assessment in southwestern Lake Michigan, including the identification of factors that determine yellow perch year-class strength April 1, 2001 - March 31, 2002

Issued June 2002 F-123-R-8; NOTE: Two different reports numbered 02/06 were issued from the CAE.Report issued on: June 2002INHS Technical Report prepared for Division of Fisherie

Patterns of Fish Diversity in a Mainstem Missouri River Reservoir and Associated Delta in South Dakota and Nebraska, USA

There is an expansive and expanding delta at the confluence of the Niobrara and Missouri Rivers in the Lewis and Clark Reservoir. The delta provides diverse aquatic habitat that is somewhat similar to the historic Missouri River and to remnant river habitats. As such, the delta may have relatively high fish species diversity compared to lentic reservoir habitats. To compare patterns of fish diversity between the delta and reservoir habitats, we collected fish in several nursery habitats in both areas using four gear types (seine, gill net, electrofisher and fyke net) on three occasions (July, August and September) in 2005. Species richness was higher in the delta (n=34) than the reservoir (n=22). Thirteen species composed more than 1% of delta collections while only four species composed more than 1% of reservoir collections. Species diversity (Fisher’s a) was also significantly higher in the delta. Higher species diversity in the delta may be explained by higher habitat diversity. These results suggest that newly forming deltas have the potential to protect and restore fish species diversity, because they retain natural river functions such as sediment transport and habitat formation

USING OTOLITH MICROCHEMISTRY TO CLASSIFY YELLOW PERCH AS STOCKED OR NATURALLY PRODUCED

Fisheries managers routinely use stocking to supplement fish populations (Schramm and Piper 1995, Fisher 1996). Stocking eyed-eggs offers substantial cost savings compared to stocking fry and fingerlings (PFBC 2011); however, traditional stocking evaluation using oxytetracycline (OTC) marking of otoliths is ineffective for eyed-eggs of some species (e.g., yellow perch, [Perca fla- vescens]). Thus, there is a need for additional approaches to be able to classify fish as stocked or naturally produced. Fish otoliths are paired calcified structures in the inner ear that permanently deposit trace elements in proportion to water column concentrations (Campana 1999, Campana et al. 2000). Coupled with otolith growth increments (i.e., annuli), elemental accumulation permits retrospective evaluation of environmental history (e.g., natal origins, movement) if water chemistry is spatially heterogeneous and temporally constant (Elsdon et al. 2008). Otolith microchemistry can be used to evaluate stocking contributions (Pracheil et al. 2014) and in the context of eyed-egg stockings, may be useful for classifying fish as stocked or naturally produced. Yellow perch is a popular sport fish species in South Dakota (Gigliotti 2007) and is routinely stocked by fisheries managers to supplement weak year classes (Schoene- beck et al. 2010). The South Dakota Department of Game, Fish and Parks (SDGFP) propagates yellow perch for stocking (e.g., eyed-eggs, fry, fingerlings) and also stocks adult perch through trap and transfer operations (Lott 1991, Fisher 1996). However, the contributions of yellow perch stockings in South Dakota are largely unknown because it is difficult to differentiate stocked fish from resident individuals (Brown and St. Sauver 2002). Our objective was to assess the utility of otolith microchemistry to distinguish hatchery-reared yellow perch stocked at the eyed-egg stage from naturally produced individuals

Otolith Microchemistry Reveals Natal Origins of Walleyes in Missouri River Reservoirs

Reproductive habitats are vital for sustaining fish populations, but their location and relative natal contributions are often unknown or poorly understood. We used otolith microchemistry to examine natal origins of Walleyes Sander vitreus in Missouri River reservoirs (i.e., Lake Oahe, Lake Sharpe, Lake Francis Case, and Lewis and Clark Lake) in North Dakota and South Dakota. Water Sr:Ca and Ba:Ca were spatially heterogeneous and temporally consistent in all impoundments. Otolith Sr:Ca and Ba:Ca from age-0 Walleyes permitted the reclassification of fish to known natal habitats (i.e., tributary, embayment, main stem) and individual sites with 87% and 75% accuracy, respectively. Natal contributions were highest in tributaries, particularly those in Lake Oahe, where 32% of all adults and 77% of Lake Oahe adults hatched. Embayments and main-stem environments had high natal contributions (67–78%) in Lakes Sharpe and Francis Case and Lewis and Clark Lake, where tributaries are less abundant. Our research demonstrates the utility of otolith microchemistry for measuring habitat- and site-specific natal contributions and provides further information that can be used in managing Walleyes in Missouri River reservoirs, particularly for broodstock collection, habitat protection and restoration, and harvest regulations

Size-Dependent Trophic Patterns of Pallid Sturgeon and Shovelnose Sturgeon in a Large River System

This study compared patterns of d15N and d13C enrichment of pallid sturgeon Scaphirhynchus albus and shovelnose sturgeon S. platorynchus in the Missouri River, United States, to infer their trophic position in a large river system. We examined enrichment and energy flow for pallid sturgeon in three segments of the Missouri River (Montana/North Dakota, Nebraska/South Dakota, and Nebraska/Iowa) and made comparisons between species in the two downstream segments (Nebraska/South Dakota and Nebraska/Iowa). Patterns in isotopic composition for pallid sturgeon were consistent with gut content analyses indicating an ontogenetic diet shift from invertebrates to fish prey at sizes of .500-mm fork length (FL) in all three segments of the Missouri River. Isotopic patterns revealed shovelnose sturgeon did not experience an ontogenetic shift in diet and used similar prey resources as small (,500-mm FL) pallid sturgeon in the two downstream segments. We found stable isotope analysis to be an effective tool for evaluating the trophic position of sturgeons within a large river food web

Walleye Trophic Position Before and After a Gizzard Shad Extirpation

Walleye (Sander vitreus) are an ecologically and recreationally important sport fish species. Reduced growth and condition in walleye can occur when prey availability is limited. In two Nebraska reservoirs, walleye consumed gizzard shad (Dorosoma cepedianum) as their primary prey until a winterkill extirpated the gizzard shad in 2001. Because of the winterkill, walleye in the two reservoirs had to change to alternative prey items. Our objective was to determine if stable isotope analysis on archived walleye scales can be used to detect a known food web shift in two reservoir food webs. We quantified the changes in walleye trophic position following the loss of gizzard shad using stable isotope analysis of carbon (δ13C) and nitrogen (δ15N) from archived scales. Walleye δ15N decreased and δ13C increased in both reservoirs after the extirpation of gizzard shad, indicating walleye likely fed at a lower trophic level on more benthic or littoral prey resources post winterkill. A replacement of gizzard shad by white perch (Morone americana) in Pawnee Reservoir may have ameliorated the loss of gizzard shad; in the other system, walleye appeared to feed on a wider variety of prey items as indexed by increased δ13C variability. Our results indicated that walleye were robust to gizzard shad extirpation

Effects of Historic Flooding on Fishes and Aquatic Habitats in a Missouri River Delta

Understanding the effects of disturbances on aquatic biota is important for management of the world\u27s rivers. Riverine deltas are among the most biologically productive ecosystems, yet the ecological effects of floods in deltas are poorly understood. Therefore, the goal of this study was to examine impacts of an historic flood in the Missouri River basin in 2011 on fishes and aquatic habitats in the Lewis and Clark Delta, located in South Dakota and Nebraska, USA. Fish community structural indices declined in the six years preceding the flood, with species richness and diversity (Fisher\u27s α) decreasing from 25 to 22 and 4.56 to 3.48, respectively. However, the fish community exhibited short-term resistance to the flood as both metrics were similar to pre-flood levels after the disturbance in 2012. Evenness (J′) declined from 0.88 to 0.73 before the flood but increased to 0.84 after the disturbance. The majority of species exhibited greater relative abundance after the flood regardless of age class (i.e., juvenile, adult), morphology (i.e., small-bodied, large-bodied), introduction history (i.e., introduced, native), or recreational importance (i.e., sport fish, non-recreational). However, the flood reduced relative abundance of juvenile freshwater drum (Aplodinotus grunniens) and white crappie (Pomoxis annularis) and did not affect the three small-bodied species that were studied, including emerald shiner (Notropis atherinoides), red shiner (Cyprinella lutrensis), and spotfin shiner (Cyprinella spiloptera). The flood decreased side channel and backwater frequency and width/area but increased sandbar abundance. Physical alterations evidently had minimal effects on structural indices of the fish community. However, an overall increase in relative abundance across species suggests that interspersed fluvial and slackwater habitats in the delta provided refuge from floodwaters during the disturbance. Maintaining habitat connectivity in deltas during and after floods is particularly important for fisheries conservation. Illustrating the ecological effects and implications of a major flood, this study contributes to the nascent field of delta ecology

Effects of the 2011 Missouri River flood on walleye natal recruitment and habitat use in Lake Sharpe, South Dakota

Floods affect fish populations in the short term (e.g. entrainment, mortality) and long term (e.g. recruitment, habitat availability), but oftentimes long-term effects are overlooked. In 2011, a catastrophic flood with record peak flows (4,200 m3/s) occurred in Lake Sharpe, a mainstem Missouri River reservoir. The flood’s immediate impacts on the Lake Sharpe walleye (Sander vitreus) population have been documented, but long-term effects on natal recruitment and habitat use have not been assessed. We used otolith chemistry to evaluate spatial patterns in walleye natal recruitment and late-summer (i.e. July − September) habitat use in years before, during, and after the flood to gain insight into long-term flood impacts on the Lake Sharpe walleye population. From 2004 to 2013, all walleye hatched in embayments and main channel habitats as opposed to stilling basins or tributaries, and the majority of age ≥ 1 fish (i.e. age-1 or older) used the former habitats in late summer. During the flood year, natal recruitment remained stable relative to pre-flood levels in embayments (26% pre-flood, 20% during flood) and main channels (74% pre-flood, 80% during flood). Habitat use of age ≥ 1 walleye was generally unaltered by the flood as it remained consistent in embayments and main channel habitats before, during, and after the disturbance. Hence, the Lake Sharpe walleye population was largely unaffected by the flood in terms of natal recruitment and habitat use. Our results highlight the resilience of the Lake Sharpe walleye population to a catastrophic flood, providing fisheries professionals with an otolith chemistry approach for quantifying flood effects on natal recruitment and habitat use and developing spatially informed management approaches (e.g. habitat protection/rehabilitation, harvest regulations)