Liquid-cooled modular gas cell system for high-order harmonic generation using high average power laser systems

We present the design and implementation of a new, modular gas target suitable for high-order harmonic generation using high average power lasers. To ensure thermal stability in this high heat load environment, we implement an appropriate liquid cooling system. The system can be used in multiple-cell configurations, allowing us to control the cell length and aperture size. The cell design was optimized with heat and flow simulations for thermal characteristics, vacuum compatibility, and generation medium properties. Finally, the cell system was experimentally validated by conducting high-order harmonic generation measurements using the 100 kHz high average power HR-1 laser system at the Extreme Light Infrastructure Attosecond Light Pulse Source (ELI ALPS) facility. Such a robust, versatile, and stackable gas cell arrangement can easily be adapted to different experimental geometries in both table-top laboratory systems and user-oriented facilities, such as ELI ALPS

All-Optical Experimental Control of High-Harmonic Photon Energy

We generate high-order harmonics in gaseous medium with tunable photon energy using time domain interferometry of double pulses in a non-collinear generation geometry. The method is based on the fact that the generated harmonics inherit certain spectral properties of the driving laser. The two temporally delayed ultrashort laser pulses, identical in all parameters, are produced by a custom-made split-and-delay unit utilizing wave front splitting without a significant energy loss. The arrangement is easy to implement in any attosecond pulse generation beamline, and is suitable for the production of an extreme ultraviolet source with simply and quickly variable central photon energy, useful for a broad range of applications.Comment: 6 pages, 5 figures, after peer-revie

Riluzole treatment modulates KCC2 and EAAT-2 receptor expression and Ca2+ accumulation following ventral root avulsion injury

Avulsion injury results in motoneuron death due to the increased excitotoxicity developing in the affected spinal segments. This study focused on possible short and long term molecular and receptor expression alterations which are thought to be linked to the excitotoxic events in the ventral horn with or without the anti-excitotoxic riluzole treatment. In our experimental model the left lumbar 4 and 5 (L4, 5) ventral roots of the spinal cord were avulsed. Treated animals received riluzole for 2 weeks. Riluzole is a compound that acts to block voltageactivated Na+ and Ca2+ channels. In control animals the L4, 5 ventral roots were avulsed without riluzole treatment. Expression of astrocytic EAAT-2 and that of KCC2 in motoneurons on the affected side of the L4 spinal segment were detected after the injury by confocal and dSTORM imaging, intracellular Ca2+ levels in motoneurons were quantified by electron microscopy. The KCC2 labeling in the lateral and ventrolateral parts of the L4 ventral horn was weaker compared with the medial part of L4 ventral horn in both groups. Riluzole treatment dramatically enhanced motoneuron survival but was not able to prevent the down-regulation of KCC2 expression in injured motoneurons. In contrast, riluzole successfully obviated the increase of intracellular calcium level and the decrease of EAAT-2 expression in astrocytes compared with untreated injured animals. We conclude that KCC2 may not be an essential component for survival of injured motoneurons and riluzole is able to modulate the intracellular level of calcium and expression of EAAT-2

Spectrally tunable ultrashort monochromatized extreme ultraviolet pulses at 100 kHz

We present the experimental realization of spectrally tunable, ultrashort, quasimonochromatic extreme ultraviolet (XUV) pulses generated at 100 kHz repetition rate in a user-oriented gas high harmonic generation (GHHG) beamline of the Extreme Light Infrastructure - Attosecond Light Pulse Source (ELI ALPS) facility. Versatile spectral and temporal shaping of the XUV pulses are accomplished with a double-grating, time-delay compensated monochromator accommodating the two composing stages in a novel, asymmetrical geometry. This configuration supports the achievement of high monochromatic XUV flux (2.8e10+/-0.9e10 photons/s) combined with ultrashort pulse duration (4.0+/-0.2 fs using 12.1+/-0.6 fs driving pulses) and small spot size (sub-100 um). Focusability, spectral bandwidth, and overall photon flux of the produced radiation were investigated covering a wide range of instrumental configurations. Moreover, complete temporal (intensity and phase) characterization of the few-femtosecond monochromatic XUV pulses - a goal that is difficult to achieve by conventional reconstruction techniques - has been realized using ptychographic algorithm on experimentally recorded XUV-IR pump-probe traces. The presented results contribute to in-situ, time-resolved experiments accessing direct information on the electronic structure dynamics of novel target materials.Comment: 20 pages, 8 figure

Advanced laboratory exercise: studying the dispersion properties of a prism pair

A laboratory exercise has been developed in the frames of a new course called "Advanced undergraduate laboratory in femtosecond optics", which aims to study the dependence of the dispersion of a prism pair on the positions of the prisms. For the dispersion measurement we chose a relatively simple technique, called white light spectral interferometry. The prism pair consisting of two identical fused silica prisms was placed in the sample arm of a Michelson- interferometer illuminated with a tungsten halogen lamp. The interferograms were observed with a low resolution spectrometer in order to have a wide detection range (200- 1100 nm). Measurements were performed by adjusting the optical path length in the second prism. The data was evaluated with the cosine-function fit method. Using the formalism of Fork the phase derivatives were theoretically calculated as well. The dependence of the dispersion coefficients on the displacement of the second prism agree well with the measurements. Using white light is advantageous as its broad wavelength range facilitates the retrieval of the spectral phase with high precision in a wide range. Furthermore, white light sources are relatively low-cost and safe in contrast to ultrashort laser sources. © COPYRIGHT SPIE