First record of black corals (Antipatharia) in shallow coastal waters of northern Chile by means of underwater video

This record is the first report of black corals (Antipatharia) in shallow waters of the continental coast of Chile, and extends their geographical range in shallow waters of the upper continental shelf of South America by ~3000 km from Ecuador (~1°S) to Coquimbo, Chile (~29°S). Specimens were observed between 70 and 107 m on three underwater video transects executed in July 2016 on the rocky reef El Toro, which is located about 65 km northwest of Coquimbo. The images were taken with the cameras of a remotely operated vehicle (ROV) at a distance of about two meters from the rocks. Although the image resolution does not allow an exact identification of the species, the multi-branched corals were distinguished from other cnidarians as Antipatharia by the dark color of the stem

12^{12}C/13^{13}C ratio in planetary nebulae from the IUE archives

We investigated the abundance ratio of $^{12}$C/$^{13}$C in planetary nebulae by examining emission lines arising from \ion{C}{3} 2s2p ^3P_{2,1,0} \to 2s^2 ^1S_0. Spectra were retrieved from the International Ultraviolet Explorer archives, and multiple spectra of the same object were coadded to achieve improved signal-to-noise. The $^{13}$C hyperfine structure line at 1909.6 \AA was detected in NGC 2440. The $^{12}$C/$^{13}$C ratio was found to be $\sim4.4\pm$1.2. In all other objects, we provide an upper limit for the flux of the 1910 \AA line. For 23 of these sources, a lower limit for the $^{12}$C/$^{13}$C ratio was established. The impact on our current understanding of stellar evolution is discussed. The resulting high signal-to-noise \ion{C}{3} spectrum helps constrain the atomic physics of the line formation process. Some objects have the measured 1907/1909 flux ratio outside the low-electron density theoretical limit for $^{12}$C. A mixture of $^{13}$C with $^{12}$C helps to close the gap somewhat. Nevertheless, some observed 1907/1909 flux ratios still appear too high to conform to the presently predicted limits. It is shown that this limit, as well as the 1910/1909 flux ratio, are predominantly influenced by using the standard partitioning among the collision strengths for the multiplet $^1S_0$--$^3P_J$ according to the statistical weights. A detailed calculation for the fine structure collision strengths between these individual levels would be valuable.Comment: ApJ accepted: 19 pages, 3 Figures, 2 Table

Kinematic and morphological modeling of the bipolar nebula Sa2-237

We present [OIII]500.7nm and Halpha+[NII] images and long-slit, high resolution echelle spectra in the same spectral regions of Sa2--237, a possible bipolar planetary nebula. The image shows a bipolar nebula of about 34" extent, with a narrow waist, and showing strong point symmetry about the central object, indicating it's likely binary nature. The long slit spectra were taken over the long axis of the nebula, and show a distinct ``eight'' shaped pattern in the velocity--space plot, and a maximum projected outflow velocity of V=106km/s, both typical of expanding bipolar planetary nebulae. By model fitting the shape and spectrum of the nebula simultaneously, we derive the inclination of the long axis to be 70 degrees, and the maximum space velocity of expansion to be 308 km/s. Due to asymmetries in the velocities we adopt a new value for the system's heliocentric radial velocity of -30km/s. We use the IRAS and 21cm radio fluxes, the energy distribution, and the projected size of Sa2-237 to estimate it's distance to be 2.1+-0.37kpc. At this distance Sa2-237 has a luminosity of 340 Lsun, a size of 0.37pc, and -- assuming constant expansion velocity -- a nebular age of 624 years. The above radial velocity and distance place Sa2--237 in the disk of the Galaxy at z=255pc, albeit with somewhat peculiar kinematics.Comment: 10pp, 4 fig

First Ecological Characterization of Whip Black Coral Assemblages (Hexacorallia: Antipatharia) in the Easter Island Ecoregion, Southeastern Pacific

One of the main priorities of marine scientific research is to provide practical information and guidance for biodiversity conservation and management. In this context, the identification of key and fragile habitat-forming species is particularly important. Despite their ecological importance, whip corals in the order Antipatharia remain poorly studied around the world, and especially in the South Pacific. During recent expeditions to explore mesophotic and deep benthic habitats around Rapa Nui (Easter Island), dense assemblages of whip black corals were observed in situ. We here report the first detailed description of the distribution and abundance of these black coral assemblages and their associated fauna and habitat. These descriptions were based on analysis of video surveys collected on 67 remotely operated vehicle dives conducted in 2014 through 2019 at three sites (Pukao seamount, Apolo peak, and Rapa Nui Island shelf and slope) within the Easter Island Ecoregion at depths between 50 and 330 m. At least four morphotypes (yellow, red, white/orange, and Pukao) of unbranched black corals were detected. Each morphotype had polyps arranged in a single row on only one side of the corallum, a diagnostic morphological character currently assigned to the antipatharian genus Stichopathes. Samples of the yellow and red morphotypes were collected and studied using morphological and molecular techniques. These analyses indicate that the genus Stichopathes is not monophyletic and requires further revisions, a conclusion that is consistent with previous studies. Extensive fields of whip black corals, primarily of the yellow and red morphotype, were present at 28 sites from 60 to 245 m on rocky substrates, coarse sand, or rhodoliths. Densities of these assemblages per frame were up to 58.2 ± 5.2 colonies m–2 (average: 13.2 ± 0.8), which represent some of the highest reported densities for black corals to date. Numerous faunal groups, including ophiuroids, echinoids, and fishes, were associated with these whip black coral assemblages. Our observations should be considered in future management and conservation efforts around Rapa Nui, where a multi-use marine protected area was established in 2018, as well as in adjacent international waters that remain unprotected but likely harbor similar assemblages

High-velocity collimated outflows in planetary nebulae: NGC 6337, He 2-186, and K 4-47

We have obtained narrow-band images and high-resolution spectra of the planetary nebulae NGC 6337, He 2-186, and K 4-47, with the aim of investigating the relation between their main morphological components and several low-ionization features present in these nebulae. The data suggest that NGC 6337 is a bipolar PN seen almost pole on, with polar velocities higher than 200 km/s. The bright inner ring of the nebula is interpreted to be the "equatorial" density enhancement. It contains a number of low-ionization knots and outward tails that we ascribe to dynamical instabilities leading to fragmentation of the ring or transient density enhancements due to the interaction of the ionization front with previous density fluctuations in the ISM. The lobes show a pronounced point-symmetric morphology and two peculiar low-ionization filaments whose nature remains unclear. The most notable characteristic of He 2-186 is the presence of two high-velocity (higher than 135 km/s) knots from which an S-shaped lane of emission departs toward the central star. K 4-47 is composed of a compact core and two high-velocity, low-ionization blobs. We interpret the substantial broadening of line emission from the blobs as a signature of bow shocks, and using the modeling of Hartigan, Raymond, & Hartman (1987), we derive a shock velocity of 150 km/s and a mild inclination of the outflow on the plane of the sky. We discuss possible scenarios for the formation of these nebulae and their low-ionization features. In particular, the morphology of K 4-47 hardly fits into any of the usually adopted mass-loss geometries for single AGB stars. Finally, we discuss the possibility that point-symmetric morphologies in the lobes of NGC 6337 and the knots of He 2-186 are the result of precessing outflows from the central stars.Comment: 16 pages plus 7 figures, ApJ accepted. Also available at http://www.iac.es/publicaciones/preprints.htm

Pre-Clinical Evaluation of a 213Bi-Labeled 2556 Antibody to HIV-1 gp41 Glycoprotein in HIV-1 Mouse Models as a Reagent for HIV Eradication

Any strategy for curing HIV infection must include a method to eliminate viral-infected cells. Based on our earlier proof-of-principle results targeting HIV-1 infected cells with radiolabeled antibody (mAb) to gp41 viral antigen, we embarked on identifying a suitable candidate mAb for preclinical development.Among the several human mAbs to gp41 tested, mAb 2556 was found to have high affinity, reactivity with multimeric forms of gp41 present on both the surface of virus particles and cells expressing HIV-1 Env, and recognition of a highly conserved epitope of gp41 shared by all HIV-1 subtypes. Also, mAb 2556 was the best in competition with HIV-1+ serum antibodies, which is an extremely important consideration for efficacy in the treatment of HIV patients. When radiolabeled with alpha-emitting radionuclide 213-Bismuth ((213)Bi) - (213)Bi-2556 efficiently and specifically killed ACH-2 human lymphocytes chronically infected with HIV-1, and HIV-1 infected human peripheral blood mononuclear cells (hPBMCs). The number of binding sites for (213)Bi-2556 on the surface of the infected cells was >10(6). The in vivo experiments were performed in two HIV-1 mouse models--splenic and intraperitoneal. In both models, the decrease in HIV-1 infected hPBMCs from the spleens and peritoneum, respectively, was dose-dependent with the most pronounced killing of hPBMCs observed in the 100 µCi (213)Bi-2556 group (P = 0.01). Measurement of the blood platelet counts and gross pathology of the treated mice demonstrated the lack of toxicity for (213)Bi-2556.We describe the preclinical development of a novel radiolabeled mAb reagent that could potentially be part of an HIV eradication strategy that is ready for translation into the clinic as the next step in its development. As viral antigens are very different from "self" human antigens - this approach promises high selectivity, increased efficacy and low toxicity, especially in comparison to immunotoxins

Widespread but regionally specific effects of experimenter- versus self-administered morphine on dendritic spines in the nucleus accumbens, hippocampus, and neocortex of adult rats

We studied the effects of self-administered (SA) vs. experimenter-administered (EA) morphine on dendritic spines in the hippocampal formation (CA1 and dentate), nucleus accumbens shell (NAcc-s), sensory cortex (Par1 and Oc1), medial frontal cortex (Cg3), and orbital frontal cortex (AID) of rats. Animals in the SA group self-administered morphine in 2-h sessions (0.5 mg/kg/infusion, i.v.) for an average of 22 sessions and animals in the EA group were given daily i.v. injections of doses that approximated the total session dose for matched rats in Group SA (average cumulative dose/session of 7.7 mg/kg). Control rats were given daily i.v. infusions of saline. One month after the last treatment the brains were processed for Golgi-Cox staining. In most brain regions (Cg3, Oc1, NAcc-s) morphine decreased the density of dendritic spines, regardless of mode of administration (although to a significantly greater extent in Group SA). However, only SA morphine decreased spine density in the hippocampal formation and only EA morphine decreased spine density in Par1. Interestingly, in the orbital frontal cortex morphine significantly increased spine density in both Groups SA and EA, although to a much greater extent in Group SA. We conclude: 1) Morphine has persistent (at least 1 month) effects on the density of dendritic spines in many brain regions, and on many different types of cells (medium spiny neurons, pyramidal cells, and granule cells); 2) The effect of morphine on spine density (and presumably synaptic organization) varies as a function of both brain region and mode of drug administration; and 3) The ability of morphine to remodel synaptic inputs in a regionally specific manner may account for the many different long-term sequelae associated with opioid use. Synapse 46:271–279, 2002. © 2002 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34996/1/10146_ftp.pd

Anti-V3 Monoclonal Antibodies Display Broad Neutralizing Activities against Multiple HIV-1 Subtypes

Background: The V3 loop of the HIV-1 envelope (Env) glycoprotein gp120 was identified as the “principal neutralizing domain” of HIV-1, but has been considered too variable to serve as a neutralizing antibody (Ab) target. Structural and immunochemical data suggest, however, that V3 contains conserved elements which explain its role in binding to virus co-receptors despite its sequence variability. Despite this evidence of V3 conservation, the ability of anti-V3 Abs to neutralize a significant proportion of HIV-1 isolates from different subtypes (clades) has remained controversial. Methods: HIV-1 neutralization experiments were conducted in two independent laboratories to test human anti-V3 monoclonal Abs (mAbs) against pseudoviruses (psVs) expressing Envs of diverse HIV-1 subtypes from subjects with acute and chronic infections. Neutralization was defined by 50% inhibitory concentrations (IC50), and was statistically assessed based on the area under the neutralization titration curves (AUC). Results: Using AUC analyses, statistically significant neutralization was observed by ≥1 anti-V3 mAbs against 56/98 (57%) psVs expressing Envs of diverse subtypes, including subtypes A, AG, B, C and D. Even when the 10 Tier 1 psVs tested were excluded from the analysis, significant neutralization was detected by ≥1 anti-V3 mAbs against 46/88 (52%) psVs from diverse HIV-1 subtypes. Furthermore, 9/24 (37.5%) Tier 2 viruses from the clade B and C standard reference panels were neutralized by ≥1 anti-V3 mAbs. Each anti-V3 mAb tested was able to neutralize 28–42% of the psVs tested. By IC50 criteria, 40/98 (41%) psVs were neutralized by ≥1 anti-V3 mAbs. Conclusions: Using standard and new statistical methods of data analysis, 6/7 anti-V3 human mAbs displayed cross-clade neutralizing activity and revealed that a significant proportion of viruses can be neutralized by anti-V3 Abs. The new statistical method for analysis of neutralization data provides many advantages to previously used analyses

Morphology and Composition of the Helix Nebula

We present new narrow-band filter imagery in H-alpha and [N II] 6584 along with UV and optical spectrophotometry measurements from 1200 to 9600 Angstroms of NGC 7293, the Helix Nebula, a nearby, photogenic planetary nebula of large diameter and low surface brightness. Detailed models of the observable ionized nebula support the recent claim that the Helix is actually a flattened disk whose thickness is roughly one-third its diameter with an inner region containing hot, highly ionized gas which is generally invisible in narrow-band images. The outer visible ring structure is of lower ionization and temperature and is brighter because of a thickening in the disk. We also confirm a central star effective temperature and luminosity of 120,000K and 100L_sun, and we estimate a lower limit to the nebular mass to be 0.30M_sun. Abundance measurements indicate the following values: He/H=0.12 (+/-0.017), O/H=4.60x10^-4 (+/-0.18), C/O=0.87 (+/-0.12), N/O=0.54 (+/-0.14), Ne/O=0.33 (+/-0.04), S/O=3.22x10^-3 (+/-0.26), and Ar/O=6.74x10^-3 (+/-0.76). Our carbon abundance measurements represent the first of their kind for the Helix Nebula. The S/O ratio which we derive is anomalously low; such values are found in only a few other planetary nebulae. The central star properties, the super-solar values of He/H and N/O, and a solar level of C/O are consistent with a 6.5M_sun progenitor which underwent three phases of dredge-up and hot bottom burning before forming the planetary nebula.Comment: 50-page manuscript plus 11 postscript figures. This revised version corrects a typo in earlier submission. Nothing else has changed. Accepted for publication in the Astrophysical Journa