FEW SHOT PHOTOGRAMETRY: A COMPARISON BETWEEN NERF AND MVS-SFM FOR THE DOCUMENTATION OF CULTURAL HERITAGE

3D documentation methods for Digital Cultural Heritage (DCH) domain is a field that becomes increasingly interdisciplinary, breaking down boundaries that have long separated experts from different domains. In the past, there has been an ambiguous claim for ownership of skills, methodologies, and expertise in the heritage sciences. This study aims to contribute to the dialogue between these different disciplines by presenting a novel approach for 3D documentation of an ancient statue. The method combines TLS acquisition and MVS pipeline using images from a DJI Mavic 2 drone. Additionally, the study compares the accuracy and final product of the Deep Points (DP) and Neural Radiance Fields (NeRF) methods, using the TLS acquisition as validation ground truth. Firstly, a TLS acquisition was performed on an ancient statue using a Faro Focus 2 scanner. Next, a multi-view stereo (MVS) pipeline was adopted using 2D images captured by a Mini-2 DJI Mavic 2 drone from a distance of approximately 1 meter around the statue. Finally, the same images were used to train and run the NeRF network after being reduced by 90%. The main contribution of this paper is to improve our understanding of this method and compare the accuracy and final product of two different approaches - direct projection (DP) and NeRF - by exploiting a TLS acquisition as the validation ground truth. Results show that the NeRF approach outperforms DP in terms of accuracy and produces a more realistic final product. This paper has important implications for the field of CH preservation, as it offers a new and effective method for generating 3D models of ancient statues. This technology can help to document and preserve important cultural artifacts for future generations, while also providing new insights into the history and culture of different civilizations. Overall, the results of this study demonstrate the potential of combining TLS and NeRF for generating accurate and realistic 3D models of ancient statues

Biocompounds recovery from Spirulina by conventional and ohmic heating methodologies: chemical and biological properties

Extracting the totality of bio-compounds with industrial interest from Cyanobacterium is often prevented by the intrinsic rigidity of its cell wall. In this sense, the present study focuses on evaluating the influence thermal batch extraction (conventional extraction technologies) and ohmic heating (OH) assisted extraction (considered a greener alternative technology) in blue green microalgae Arthrospira platensis (Spirulina) cell disruption for bioactive fractions recovery. The proximal composition of Spirulina was initially determined. The maximum protein content (i.e., CPhycocyanin), total carbohydrates (TC) and total phenolic compounds (TPC) extracted in water at different times (30-120 min) and temperatures (30-51 ºC) was quantified after the conventional and OH- assisted extraction. The freeze-thawing process was used as control. The antioxidant activity (i.e., FRAP and DPPH assays) of the obtained extracts was assessed. Results showed that with the freeze-thawing process, traditionally used for the recovery of bio- compounds from Spirulina, the concentration of C-phycocyanin was approx. 42 mg/g of Spirulina, 26 mgGlcE/g Spirulina of TC and 9 mgGAE/g Spirulina of TPC. Using OH-assisted extraction, the maximum of C-Phycocyanin content obtained was 45 mg/g of Spirulina (obtained at 37 ºC, 30 min), the maximum carbohydrates content was 40 mgGlcE/g Spirulina and the maximum TPC was 10 mgGAE/g Spirulina. On the other hand, using conventional thermal treatment it can be observed that, under the same conditions, the bioactive compounds recovery decreased to 35 mg/g, 20 mgGlcE/g Spirulina for C-phycocyanin concentration and TC (p<0.05), respectively. The concentration in phenolic compounds is not so affected, but even so the ohmic heating potentiates the extraction of these secondary metabolites. The antioxidant activity of the extracts there was not different between conventional treatments andOH. Thus, the results indicated that OH is a good alternative to conventional methods aiming at the extraction of intracellular components with a decrease in processing time and energy costs associated with the extraction process, which together with an easy upscale make OH an interesting methodology for use in the industrial production of microalgae colorants and bioactive supplements.info:eu-repo/semantics/publishedVersio

CHALLENGING ARCHITECTURES: AN INTEGRATED AND MULTIPURPOSE SURVEY FOR THE COMPLETE MAPPING OF THE EMIR PALACE IN KOGON (UZBEKISTAN)

The documentation of Cultural Heritage, and in particular architecture, is nowadays conducted with a combination of several geomatic techniques. Most of them relies on the exploitation of 3D point cloud, which is the state of art data to produce almost every output. The starting point of each recovery intervention is the survey, which enable a multidisciplinary approach for the knowledge of a building and, consequently, to preserve it for future generation. When it comes with complex architectures, however, the surveying approach is more compelling and challenging at the same time. The definition of the final accuracy requires a tidy design process that should be done a priori, and a set of strict rules need to be respected. With the advent of new automatic procedures, the number of data collected increases, but this doesn’t mean that well established method, like topography, can be neglected. This work is the demonstration of how old and new method must coexist. The present work describes the work carried out to the complete 3D reconstruction of a complex building, namely the Emir’s Palace place in Kogon, Uzbekistan. Given its dimension and decorative apparatus, maintaining a high standard of definition without losing metrological accuracy was the main challenge. The work was carried out with a combination of both Terrestrial Laser Scanner and Aerial Photogrammetry for the 3D reconstruction, and on a very accurate topographic network for the combination of produced point clouds. The result of this first phase of the work was a polygonal network with a millimetric accuracy. Besides, another important contribution of the paper lies on the exploitation of the panoramic images coming from the TLS within a photogrammetric software. Indeed, by exploiting the depth map, it was possible to produce very detailed orthophotos for the production of 1:10 scale drawings. The work, performed in a joint venture between Academia and SMEs, is an excellent example (useful to be shared within the research community dealing with 3D representation and optimization) of innovative methodologies developed to produce reliable drawings and 3D representation for different purposes, being the starting point for each kind of project

Influence of thermal and electrical effects of ohmic heating on C-phycocyanin properties and biocompounds recovery from Spirulina platensis

Spirulina platensis is interesting for the food industry due to its overall composition and high content in C-phycocyanin. However, the sensitivity of C-phycocyanin makes its extraction a delicate process. The present study focuses on assessing the use of ohmic heating (OH) in the recovery of C-phycocyanin and other relevant compounds as an alternative method to freeze-thawing or conventional heating. Different ohmic and conventional heating treatments were applied both to purified C-phycocyanin and Spirulina powder. Evaluation of fluorescence and circular dichroism showed that moderated electric fields increased C-phycocyanin stability. This was confirmed in the extraction trials which revealed that OH assisted extraction at higher temperatures (44°C), and shorter times (30min) allowed significant higher extraction yield of C-phycocyanin (45 mg/gdw Spirulina), in comparison with conventional heating and freeze-thawing. OH allowed also up to 80% higher yields in phenolic compounds and carbohydrates.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. The study was also supported by the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant (MSCA-RISE; FODIAC; 778388) and project OH2O – POCI-01-0145-FEDER-029145 (FCT and COMPETE2020). Pedro Santos is recipient of a fellowship supported by a doctoral advanced training (call NORTE-69-2015-15), funded by the European Social Fund under the scope of Norte2020 - Programa Operacional Regional do Norte (NORTE-08-5369-FSE-000036).info:eu-repo/semantics/publishedVersio

Glutamine-Derived Aspartate Biosynthesis in Cancer Cells: Role of Mitochondrial Transporters and New Therapeutic Perspectives

Aspartate has a central role in cancer cell metabolism. Aspartate cytosolic availability is crucial for protein and nucleotide biosynthesis as well as for redox homeostasis. Since tumor cells display poor aspartate uptake from the external environment, most of the cellular pool of aspar-tate derives from mitochondrial catabolism of glutamine. At least four transporters are involved in this metabolic pathway: the glutamine (SLC1A5_var), the aspartate/glutamate (AGC), the as-partate/phosphate (uncoupling protein 2, UCP2), and the glutamate (GC) carriers, the last three belonging to the mitochondrial carrier family (MCF). The loss of one of these transporters causes a paucity of cytosolic aspartate and an arrest of cell proliferation in many different cancer types. The aim of this review is to clarify why different cancers have varying dependencies on metabolite transporters to support cytosolic glutamine-derived aspartate availability. Dissecting the precise metabolic routes that glutamine undergoes in specific tumor types is of upmost importance as it promises to unveil the best metabolic target for therapeutic intervention

Rotondas red eggplant: nutritional characterization and extractionof high added value biocompounds using emerging electrotechnologies

Rotondas Red Eggplant belongs to the family of Solanum aethiopicum species, cultivated in a specific area of Potenza (Basilicata, Italy) and has gained the Protected Designation of Origin certification. Recently, Red Eggplant was associated with different biological properties, related to health benefits, as antioxidant, hypoglycemic, antihypertensive and hypolipidemic, probably due to its chemical composition. The search for "greener" technologies has been one of the focus of research, having in mind industrial applications. In this sense, electrotechnologies, such as ohmic heating (OH), have been explored, showing to have potentially lower energy costs, higher extraction yields, being less time consuming and increasing the biocompounds chemical stability. The aim of this study was to determine the chemical/nutritional composition of Red Eggplant and to assess conventional and OH methods in the extraction and recovery of the main bio-compounds present in this material. Red Eggplant was characterized for the nutritional profile (AOAC procedures): proteins, carbohydrates, total extractives, lipids, ash and minerals. The selected conditions for the extraction of the main biocompounds, for conventional and OH methods, were: solid/liquid ratio 1:10 (w:v), 80 °C, 25 min and water or mixture of 50% (v/v) ethanol/water as a solvent. Subsequently, assays were carried out to determine the content of proteins (Bradford), total carbohydrates (phenolsulfuric acid) and phenolics (Folin-Ciocalteu), and antioxidant activity (FRAP and DPPH) of the obtained extracts. Monosaccharides profile after hydrolysis and phenolics were also quantified by HPLC. Results showed that carbohydrates were the major macronutrient, followed by protein, lipids and ash. K, Ca and Mg were the major minerals. Regarding the monosaccharide composition, glucose, fructose, xylose and arabinose were the most abundant sugars. Ethanolic extractives represented approximately 46 % of the Red Eggplant composition. Independently of the solvent, higher amounts of polyphenols, proteins and carbohydrates were extracted when OH was applied. Moreover, OH extracts showed greater antioxidant activity compared to extracts obtained by the conventional extraction method. According to the polyphenol profile of the extracts the main compounds found were taxifolin, rutin and rosmarinic acid. These results demonstrate the potential of this vegetable to be used for nutraceuticals or functional food.The authors wish to thank for the financial support to funding program Marie Skłodowska-Curie grant (MSCA-RISE; FODIAC; 778388). Pedro Santos is recipient of a fellowship supported by a doctoral advanced training (call NORTE-69-2015-15), funded by the European Social Fund under the scope of Norte2020 - Programa Operacional Regional do Norte (NORTE-08-5369-FSE-000036). Zlatina Genisheva is supported by the project OH2O (POCI-01-0145-FEDER-029145) funded by FCT and Fundo Europeu de Desenvolvimento Regional (FEDER) under the scope of Programa Operacional de Competividade e Internacionalizaçao (POCI)-COMPETE 2020 and Portugal 2020.info:eu-repo/semantics/publishedVersio

Drosophila melanogaster Uncoupling Protein-4A (UCP4A) Catalyzes a Unidirectional Transport of Aspartate

Uncoupling proteins (UCPs) form a distinct subfamily of the mitochondrial carrier family (MCF) SLC25. Four UCPs, DmUCP4A-C and DmUCP5, have been identified in Drosophila melanogaster on the basis of their sequence homology with mammalian UCP4 and UCP5. In a Parkinson’s disease model, DmUCP4A showed a protective role against mitochondrial dysfunction, by increasing mitochondrial membrane potential and ATP synthesis. To date, DmUCP4A is still an orphan of a biochemical function, although its possible involvement in mitochondrial uncoupling has been ruled out. Here, we show that DmUCP4A expressed in bacteria and reconstituted in phospholipid vesicles catalyzes a unidirectional transport of aspartate, which is saturable and inhibited by mercurials and other mitochondrial carrier inhibitors to various degrees. Swelling experiments carried out in yeast mitochondria have demonstrated that the unidirectional transport of aspartate catalyzed by DmUCP4 is not proton-coupled. The biochemical function of DmUCP4A has been further confirmed in a yeast cell model, in which growth has required an efflux of aspartate from mitochondria. Notably, DmUCP4A is the first UCP4 homolog from any species to be biochemically characterized. In Drosophila melanogaster, DmUCP4A could be involved in the transport of aspartate from mitochondria to the cytosol, in which it could be used for protein and nucleotide synthesis, as well as in the biosynthesis of ß-alanine and N-acetylaspartate, which play key roles in signal transmission in the central nervous system

New Insight on the Bioactivity of Solanum aethiopicum Linn. Growing in Basilicata Region (Italy): Phytochemical Characterization, Liposomal Incorporation, and Antioxidant Effects

Food extract’s biological effect and its improvement using nanotechnologies is one of the challenges of the last and the future decades; for this reason, the antioxidant effect of scarlet eggplant extract liposomal incorporation was investigated. Scarlet eggplant (Solanum aethiopicum L.) is a member of the Solanaceae family, and it is one of the most consumed vegetables in tropical Africa and south of Italy. This study investigated the antioxidant activity and the phytochemical composition of S. aethiopicum grown in the Basilicata Region for the first time. The whole fruit, peel, and pulp were subjected to ethanolic exhaustive maceration extraction, and all extracts were investigated. The HPLC-DAD analysis revealed the presence of ten phenolic compounds, including hydroxycinnamic acids, flavanones, flavanols, and four carotenoids (one xanthophyll and three carotenes). The peel extract was the most promising, active, and the richest in specialized metabolites; hence, it was tested on HepG2 cell lines and incorporated into liposomes. The nanoincorporation enhanced the peel extract’s antioxidant activity, resulting in a reduction of the concentration used. Furthermore, the extract improved the expression of endogenous antioxidants, such as ABCG2, CAT, and NQO1, presumably through the Nrf2 pathway

Misure di radioattività naturale nel sito preistorico di Grotta dei Cervi in Porto Badisco (LE)

Nel presente lavoro sono riportati I risultati di una campagna di misure finalizzata alle determinazione della concentrazione di radon all’interno di Grotta dei Cervi di Porto Badisco (LE). Grotta dei Cervi è una cavità carsica caratterizzata da un complesso pittorico neolitico imponente