Estrategias de gestión sostenible de los ecosistemas costeros del Mar Negro

El artículo aborda las características geobotánicas de áreas clave a lo largo de la costa del Mar Negro, centrándose en la región desde Novorossiysk hasta Kabardinka y desde la granja Betta hasta la bahía de Inal (Rusia). El estudio evalúa la condición de las poblaciones de plantas, evalúa las perturbaciones de la comunidad causadas por varios factores y examina los patrones de vegetación en relación con la elevación. Un motivo de gran preocupación es el impacto de las condiciones naturales y las actividades humanas en estos ecosistemas costeros. El artículo subraya la necesidad de estrategias de gestión efectivas para mitigar las consecuencias perjudiciales de la intervención humana y salvaguardar la biodiversidad de la región. El estudio subraya la importancia de las prácticas sostenibles y el turismo ecológico como parte de los enfoques de gestión responsable para mantener el atractivo y la integridad ecológica de la costa del Mar Negro. En conclusión, los conocimientos del artículo sobre los aspectos geobotánicos de la costa del Mar Negro ofrecen una valiosa orientación para las estrategias de gestión que pueden armonizar las actividades humanas con la protección y restauración de estos ecosistemas costeros vitales

A Cytosolic Multiprotein Complex Containing p85\u3cem\u3eα\u3c/em\u3e is Required for \u3cem\u3eβ\u3c/em\u3e-Catenin Activation in Colitis and Colitis-Associated Cancer

Wnt/β-catenin signaling is required for crypt structure maintenance. We previously observed nuclear accumulation of Ser-552 phosphorylated β-catenin (pβ-CatSer-552) in intestinal epithelial cells (IEC) during colitis and colitis-associated cancer. Data here delineate a novel multiprotein cytosolic complex (MCC) involved in β-catenin signaling in the intestine. The MCC contains p85α, the class IA subunit of PI3K, along with β-catenin, 14-3-3ζ, Akt, and p110α. MCC levels in IEC increase in colitis and colitis-associated cancer patients. IEC-specific p85α-deficient (p85ΔIEC) mice develop more severe dextran sodium sulfate colitis due to delayed ulcer healing and reduced epithelial β-catenin activation. In colonic IEC, p85α deficiency did not alter PI3K signaling. In vitro shRNA depletion of individual complex members disrupts the MCC and reduces β-catenin signaling. Despite worse colitis, p85ΔIEC mice have reduced tumor burden after azoxymethane/dextran sodium sulfate treatment. Together the data indicate that the β-catenin MCC is needed for mucosal repair and carcinogenesis. This novel MCC may be an attractive therapeutic target in preventing cancer in colitis patients

Myo-Inositol Reduces β-Catenin Activation in Colitis

AIM To assess dietary myo-inositol in reducing stem cell activation in colitis, and validate pβ-cateninS552 as a biomarker of recurrent dysplasia. METHODS We examined the effects of dietary myo-inositol treatment on inflammation, pβ-cateninS552 and pAkt levels by histology and western blot in IL-10-/- and dextran sodium sulfate-treated colitic mice. Additionally, we assessed nuclear pβ-cateninS552 in patients treated with myo-inositol in a clinical trial, and in patients with and without a history of colitis-induced dysplasia. RESULTS In mice, pβ-cateninS552 staining faithfully reported the effects of myo-inositol in reducing inflammation and intestinal stem cell activation. In a pilot clinical trial of myo-inositol administration in patients with a history of low grade dysplasia (LGD), two patients had reduced numbers of intestinal stem cell activation compared to the placebo control patient. In humans, pβ-cateninS552 staining discriminated ulcerative colitis patients with a history of LGD from those with benign disease. CONCLUSION Enumerating crypts with increased numbers of pβ-cateninS552 - positive cells can be utilized as a biomarker in colitis-associated cancer chemoprevention trials

Anterior bridging bronchus

Previously reported patients with a bridging bronchus (BB) presented with respiratory distress. In addition, each patient had one or more associated anomalies. All but two patients progressed to cardiopulmonary failure and death. We describe a case of an anterior BB without associated anomalies, who did well without operative intervention. This patient presented with a cough at 6 months of age. Chest X-ray was normal, but due to suspicion of foreign body aspiration, bronchoscopy was performed, which revealed a third bronchus at the carina. Bronchography demonstrated the anatomy of the BB. The patient has continued to do well without further intervention. Pediatr Pulmonol. 2003; 35:70–72. © 2003 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/35321/1/10205_ftp.pd

Beta-catenin cleavage enhances transcriptional activation

Nuclear activation of Wnt/β-catenin signaling is required for cell proliferation in inflammation and cancer. Studies from our group indicate that β-catenin activation in colitis and colorectal cancer (CRC) correlates with increased nuclear levels of β-catenin phosphorylated at serine 552 (pβ-Cat552). Biochemical analysis of nuclear extracts from cancer biopsies revealed the existence of low molecular weight (LMW) pβ-Cat552, increased to the exclusion of full size (FS) forms of β-catenin. LMW β-catenin lacks both termini, leaving residues in the armadillo repeat intact. Further experiments showed that TCF4 predominantly binds LMW pβ-Cat552 in the nucleus of inflamed and cancerous cells. Nuclear chromatin bound localization of LMW pβ-Cat552 was blocked in cells by inhibition of proteasomal chymotrypsin-like activity but not by other protease inhibitors. K48 polyubiquitinated FS and LMW β-catenin were increased by treatment with bortezomib. Overexpressed in vitro double truncated β-catenin increased transcriptional activity, cell proliferation and growth of tumor xenografts compared to FS β-catenin. Serine 552-> alanin substitution abrogated K48 polyubiquitination, β-catenin nuclear translocation and tumor xenograft growth. These data suggest that a novel proteasome-dependent posttranslational modification of β-catenin enhances transcriptional activation. Discovery of this pathway may be helpful in the development of diagnostic and therapeutic tools in colitis and cancer

Professional eco-efficient equipment and eco-responsible restaurants

Nowadays, more and more restaurants are considered to be eco-efficient. Therefore, professional kitchen equipment has a great significance. The following research observes and tests different models of modern kitchen equipment in order to prove their eco-efficiency. The producer of chosen equipment is Metos. This company manufactures large list of various devic-es for professional kitchens. Thus, in the beginning, literature review of that equipment was ful-filled. Practical part of this thesis was conducted in the Talli restaurant, which has obtained Nordic Ecolabel. Each experiment was held in a training kitchen, where all necessary equipment is availa-ble. The research is based on the experiments that were done in approximately one month, during this time 8 diverse types of dishes were prepared. Different programs and settings of equipment, e.g. manual or automatic, and different ways of preparing the same dishes were combined so as to find out which method works better. All the data concerning electricity and water consumption, as well as about timing was taken down and analysed at the end. The stated aim was achieved, as the experiments proved that professional kitchen equipment is more efficient than conventional one. Furthermore, the research suggests recommendations that tell which kind of equipment is suitable for certain types of dishes. This research has some importance for the Hospitality sector, as it provides essential analysis of professional kitchen equipment that can be applied by restaurants, which are interested in reduc-tion of electricity and water costs, and want to be eco-efficient

Indigenizing Water Governance within Treaty Lands and Territory: Development of Mississaugas of the Credit First Nation Water Framework

This research asked 'how can water governance be Indigenized within a social justice framework'?' in response to Canadian water governance injustices for Indigenous peoples. It applied this question to the Mississaugas of the Credit First Nation’s (MCFN) need for developing a water framework to Indigenize water governance within its treaty lands and territory, as a partial resolution to its 2016 Water Claim (Aboriginal Title Claim to Waters within the Traditional Lands of the Mississaugas of the New Credit). Through an emergent MCFN context-specific research methodology adapted from Kovach’s Indigenous research framework, this research explored MCFN’s water values and how they related to MCFN’s Water Claim to develop a water framework; and it aimed to decolonize constructs of social justice and western water governance. Using qualitative community-based participatory research methods, the key findings, underpinned by a literature-based conceptual framework, were: 1) MCFN’s water values were multi-faceted and interdependent within plural and intersecting Indigenous identities shaped by historical and contemporary colonial influences and Indigenous resistances; 2) The meanings of MCFN’s Water Claim, correlating to their water values, were: Healing Ourselves by reconnecting with our Anishinaabe culture; Protecting the water: having a say; and Sustaining Ourselves by reclaiming our inherent, Aboriginal title and treaty rights; 3) MCFN’s multi-dimensional Water Framework, based on the Water Claim meanings, centralizes Water is Life and embraces principles, objectives and suggested actions for MCFN’s implementation; 4) MCFN’s Water Framework as social justice for reconciliation related to MCFN’s agency in reclaiming and reconstituting its rights, culture and voice within respectful relationships and social transformations rather than Fraser’s model of economic (re)distribution, political representation and cultural recognition; and 5) MCFN’s Water Framework supports the reconceptualization of the resource-based Canadian water governance to values of interconnectedness, respect, and responsibilities. This is how MCFN sees itself Indigenizing water governance within its treaty lands and territory; and contributing to the larger water governance Indigenizing movements. The research was significant because it directly addressed a community need, expanded on Indigenous research methodologies, and decolonized western constructs to shift the power hierarchy between the colonizer and Indigenous peoples towards respectful relationships.University of GuelphSouth African National Research Foundatio