Engineering soil organic matter quality: Biodiesel Co-Product (BCP) stimulates exudation of nitrogenous microbial biopolymers

Biodiesel Co-Product (BCP) is a complex organic material formed during the transesterification of lipids. We investigated the effect of BCP on the extracellular microbial matrix or ‘extracellular polymeric substance’ (EPS) in soil which is suspected to be a highly influential fraction of soil organic matter (SOM). It was hypothesised that more N would be transferred to EPS in soil given BCP compared to soil given glycerol. An arable soil was amended with BCP produced from either 1) waste vegetable oils or 2) pure oilseed rape oil, and compared with soil amended with 99% pure glycerol; all were provided with 15N labelled KNO3. We compared transfer of microbially assimilated 15N into the extracellular amino acid pool, and measured concomitant production of exopolysaccharide. Following incubation, the 15N enrichment of total hydrolysable amino acids (THAAs) indicated that intracellular anabolic products had incorporated the labelled N primarily as glutamine and glutamate. A greater proportion of the amino acids in EPS were found to contain 15N than those in the THAA pool, indicating that the increase in EPS was comprised of bioproducts synthesised de novo. Moreover, BCP had increased the EPS production efficiency of the soil microbial community (μg EPS per unit ATP) up to approximately double that of glycerol, and caused transfer of 21% more 15N from soil solution into EPS-amino acids. Given the suspected value of EPS in agricultural soils, the use of BCP to stimulate exudation is an interesting tool to consider in the theme of delivering sustainable intensification

Effects of Late Castration and Zeranol on Growth Rate, Feed Efficiency, and Carcass and Meat Traits of Bovine Males

It is generally recognized that intact bovine males gain weight faster and require less feed per unit of gain than castrate bovine males. Further, carcasses from intact bovine males have a higher percentage of retail or edible product, but meat from intact males is generally evaluated slightly lower on palatability characteristics, particularly tenderness, than carcasses from castrate bovine males. It has been suggested that much of the advantage of intact vs castrate for rate of gain, efficiency of gain, and composition of gain may be expressed by an age of about 1 year and the disadvantages, including aggressive male behavior, that result in reduced rate and efficiency of gain, begin at about 1 year of age (at or immediately after puberty). Thus, there was need to determine the effects of castration at about 1 year on rate of gain, efficiency of gain, composition of gain, meat characteristics, and behavioral characteristics. Reports have shown that, when the anabolic agent zeranol [6-(6,10-dyhydroxyundecyl)-B-resorcyclic acid-d-lactone] is implanted in intact male calves at or before weaning, rate of gain is increased and rate of testicular growth is decreased. These experiments were conducted to determine the effects of castration and zeranol implants at 13 months of age on rate of gain, efficiency of gain, behavioral characteristics, and carcass and meat traits of bovine males

Expression of hepatocyte growth factor-like protein in human wound tissue and its biological functionality in human keratinocytes

Hepatocyte growth factor-like protein (HGFl) and its receptor, Recepteur d'Origine Nantais (RON), have been implicated in the development of wound chronicity. HGFl and RON expression was detected in acute wound tissue, chronic wound tissue and in normal skin using quantitative polymerase chain reaction (Q-PCR). HGFl and RON expression was also assessed in chronic healing and chronic non-healing wound tissues using Q-PCR and immunohistochemical staining. Expression was similarly detected in the HaCaT immortalized human keratinocyte cell line using reverse transcription polymerase chain reaction (RT-PCR). rhHGFl was used to assess the impact of this molecule on HaCaT cell functionality using in vitro growth assays and electric cell-substrate impendence sensing (ECIS) migration assays. HGFl and RON transcript expression were significantly increased in acute wound tissue compared to chronic wound tissue and were also elevated, though non-significantly, in comparison to normal skin. Minimal expression was seen in both healing and non-healing chronic wounds. Treatment of HaCaT cells with rhHGFl had no effect on growth rates but did enhance cell migration. This effect was abolished by the addition of a phospholipase C gamma (PLCγ) small molecule inhibitor. The increased expression of HGFl and RON in acute, healing wounds and the pro-migratory effect of HGFl in an in vitro human keratinocyte model, may indicate a role for HGFl in active wound healing

Symplastic solute transport and avocado fruit development : a decline in cytokinin/ABA ratio is related to appearance of the Hass small fruit variant

Studies on the effect of fruit size on endogenous ABA and isopentenyladenine (iP) in developing avocado (Persea americana Mill. cv. Hass) fruit revealed that ABA content was negatively correlated with fruit size whilst the iP/ABA ratio showed a linear relationship with increasing size of fruit harvested 226 d after full bloom. The effect of this change in hormone balance on the relationship between symplastic solute transport and appearance of the small fruit variant was examined following manipulation of the endogenous cytokinin (CK)/ABA ratio. Application of ABA caused seed coat senescence and retarded fruit growth but these effects were absent in fruit treated with equal amounts of ABA plus iP. Thus, the underlying physiological mechanisms associated with ABA-induced retardation of Hass avocado fruit growth appeared to be inextricably linked to a decline in CK content and included: diminution of mesocarp and seed coat plasmodesmatal branching, gating of mesocarp and seed coat plasmodesmata by deposition of electron dense material in the neck region, abolishment of the electrochemical gradient between mesocarp and seed coat parenchyma, and arrest of cell-to-cell chemical communication

Low- versus Mid-frequency Raman Spectroscopy for in Situ Analysis of Crystallization in Slurries

Slurry studies are useful for exhaustive polymorph and solid-state stability screening of drug compounds. Raman spectroscopy is convenient for monitoring crystallization in such slurries, as the measurements can be performed in situ even in aqueous environments. While the mid-frequency region (400-4000 cm(-1)) is dominated by intramolecular vibrations and has traditionally been used for such studies, the low-frequency spectral region (Peer reviewe

The internal structure and composition of a plate-boundary-scale serpentinite shear zone: the Livingstone Fault, New Zealand

Abstract. Deciphering the internal structure and composition of large serpentinite-dominated shear zones will lead to an improved understanding of the rheology of the lithosphere in a range of tectonic settings. The Livingstone Fault in New Zealand is a terrane-bounding structure that separates the basal portions (peridotite; serpentinised peridotite; metagabbros) of the Dun Mountain Ophiolite Belt from the quartzofeldspathic schists of the Caples and Aspiring Terrane. Field and microstructural observations from 11 localities along a strike length of ca. 140 km show that the Livingstone Fault is a steeply dipping, serpentinite-dominated shear zone tens of metres to several hundred metres wide. The bulk shear zone has a pervasive scaly fabric that wraps around fractured and faulted pods of massive serpentinite, rodingite and partially metasomatised quartzofeldspathic schist up to a few tens of metres long. S–C fabrics and lineations in the shear zone consistently indicate a steep east-side-up shear sense, with significant local dispersion in kinematics where the shear zone fabrics wrap around pods. The scaly fabric is dominated (>98 % vol) by fine-grained (≪10 µm) fibrous chrysotile and lizardite–polygonal serpentine, but infrequent (<1 % vol) lenticular relicts of antigorite are also preserved. Dissolution seams and foliation surfaces enriched in magnetite, as well as the widespread growth of fibrous chrysotile in veins and around porphyroclasts, suggest that bulk shear zone deformation involved pressure–solution. Syn-kinematic metasomatic reactions occurred along all boundaries between serpentinite, schist and rodingite, forming multigenerational networks of nephritic tremolite veins that are interpreted to have caused reaction hardening within metasomatised portions of the shear zone. We propose a conceptual model for plate-boundary-scale serpentinite shear zones which involves bulk-distributed deformation by pressure–solution creep, accompanied by a range of physical (e.g. faulting in pods and wall rocks; smearing of magnetite along fault surfaces) or chemical (e.g. metasomatism) processes that result in localised brittle deformation within creeping shear zone segments

Simulating the Biogeochemical and Biogeophysical Impacts of Transient Land Cover Change and Wood Harvest in the Community Climate System Model (CCSM4) from 1850 to 2100

This is the publisher's version, also available electronically from http://journals.ametsoc.org/doi/abs/10.1175/JCLI-D-11-00256.1.To assess the climate impacts of historical and projected land cover change in the Community Climate System Model, version 4 (CCSM4), new time series of transient Community Land Model, version 4 (CLM4) plant functional type (PFT) and wood harvest parameters have been developed. The new parameters capture the dynamics of the Coupled Model Intercomparison Project phase 5 (CMIP5) land cover change and wood harvest trajectories for the historical period from 1850 to 2005 and for the four representative concentration pathway (RCP) scenarios from 2006 to 2100. Analysis of the biogeochemical impacts of land cover change in CCSM4 reveals that the model produced a historical cumulative land use flux of 127.7 PgC from 1850 to 2005, which is in general agreement with other global estimates of 156 PgC for the same period. The biogeophysical impacts of the transient land cover change parameters were cooling of the near-surface atmosphere over land by −0.1°C, through increased surface albedo and reduced shortwave radiation absorption. When combined with other transient climate forcings, the higher albedo from land cover change was counteracted by decreasing snow albedo from black carbon deposition and high-latitude warming. The future CCSM4 RCP simulations showed that the CLM4 transient PFT parameters can be used to represent a wide range of land cover change scenarios. In the reforestation scenario of RCP 4.5, CCSM4 simulated a drawdown of 67.3 PgC from the atmosphere into the terrestrial ecosystem and product pools. By contrast the RCP 8.5 scenario with deforestation and high wood harvest resulted in the release of 30.3 PgC currently stored in the ecosystem

Enhancement of dye regeneration kinetics in dichromophoric porphyrin-carbazole triphenylamine dyes influenced by more exposed radical cation orbitals

Reduction kinetics of oxidized dyes absorbed on semiconductor surfaces and immersed in redox active electrolytes has been mainly modeled based on the free energy difference between the oxidation potential of the dye and the redox potential of the electrolyte. Only a few mechanisms have been demonstrated to enhance the kinetics by other means. In this work, the rate constant of the reduction of oxidized porphyrin dye is enhanced by attaching non-conjugated carbazole triphenylamine moiety using iodine/triiodide and tris(2,2′-bispyridinium)cobalt II/III electrolytes. These results are obtained using transient absorption spectroscopy by selectively probing the regeneration kinetics at the porphyrin radical cation and the carbazole triphenylamine radical cation absorption wavelengths. The enhancement in the reduction kinetics is not attributed to changes in the driving force, but to the more exposed dye cation radical orbitals of the dichromophoric dye. The results are important for the development of high efficiency photo-electrochemical devices with minimalized energy loss at electron transfer interfaces