Leadership Theory and Behavior: America Takes a Selfie

This paper explores the changing nature of the United States, and to some extent the world at large, and identifies major issues likely to influence and impact leadership theory and behavior in the next 50 years. The paper investigates issues related to world security, population shifts, values and morality changes, technology impact, and other social and political issues. The article emphasizes the role of the millennial generation in securing and carrying out the current or new principles defining leadership theory and behavior. The paper is an idea-generating process and connects its content to the field of problem-based learning. No solutions are given, but the article is left open for personal interpretation and thinking about leadership theory and behavior for the future

Developing Clinical Reasoning Skills in Teacher Candidates Using a Problem-Based Learning Approach

In 2013, the Educational Leadership Department at Middle Tennessee State University (MTSU) implemented a redesign of the teacher preparation program to transition from a traditional on-campus model to one delivered both on-campus and in off-campus school sites while using a problem-based learning method. This new program closely follows the medical school model of residency experiences coupled with problem-based learning events. This article describes the problembased learning process used in this program, comparing it with the early versions of medical school problem-based learning that encouraged the development of “clinical reasoning” skills. Similarities and differences are highlighted, along with key components of the learning model in use at MTSU. The article presents lessons learned and next steps to be used in implementing the problem-based learning approach in a teacher preparation setting

Looking Back, Looking Forward: A Retrospective Analysis of a Pre-service Teacher Preparation Course

This SoTL study aimed to determine thirty-eight teacher candidates’ self-efficacy after experiencing semester one of a yearlong residency. Researchers used a pre/post survey, the Teachers’ Sense of Efficacy Scale (TSES), and a retrospective pre TSES to determine perceived levels of self-efficacy in three areas: classroom management, instructional strategies, and student engagement. Findings revealed that pre TSES (candidates scored themselves at the beginning of the semester) and retrospective pre TSES scores (candidates scored themselves at the end of the semester reflecting on where they were at the beginning of the semester) were significantly different, with the retrospective pre TSES showing lower self-efficacy than the pre TSES. Post TSES comparisons to the retro-spective pre TSES showed a significant increase, whereas, the comparison of the traditional pre/post TSES showed no significant changes. This suggests teacher candidates had an overinflated sense of self-efficacy and that teacher educators should look beyond the pre TSES to provide transformational experiences for teacher candidates. The retro-pre TSES helps teacher educators monitor teacher candidates’ change in self-efficacy and gauge program impact

Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity

Foot-and-mouth disease virus (FMDV) is a significant economically and distributed globally pathogen of Artiodactyla. Current vaccines are chemically inactivated whole virus particles that require large-scale virus growth in strict bio-containment with the associated risks of accidental release or incomplete inactivation. Non-infectious empty capsids are structural mimics of authentic particles with no associated risk and constitute an alternate vaccine candidate. Capsids self-assemble from the processed virus structural proteins, VP0, VP3 and VP1, which are released from the structural protein precursor P1-2A by the action of the virus-encoded 3C protease. To date recombinant empty capsid assembly has been limited by poor expression levels, restricting the development of empty capsids as a viable vaccine. Here expression of the FMDV structural protein precursor P1-2A in insect cells is shown to be efficient but linkage of the cognate 3C protease to the C-terminus reduces expression significantly. Inactivation of the 3C enzyme in a P1-2A-3C cassette allows expression and intermediate levels of 3C activity resulted in efficient processing of the P1-2A precursor into the structural proteins which assembled into empty capsids. Expression was independent of the insect host cell background and leads to capsids that are recognised as authentic by a range of anti-FMDV bovine sera suggesting their feasibility as an alternate vaccine

Heme-Induced Macrophage Phenotype Switching and Impaired Endogenous Opioid Homeostasis Correlate with Chronic Widespread Pain in HIV

Chronic widespread pain (CWP) is associated with a high rate of disability and decreased quality of life in people with HIV-1 (PWH). We previously showed that PWH with CWP have increased hemolysis and elevated plasma levels of cell-free heme, which correlate with low endogenous opioid levels in leukocytes. Further, we demonstrated that cell-free heme impairs β-endorphin synthesis/release from leukocytes. However, the cellular mechanisms by which heme dampens β-endorphin production are inconclusive. The current hypothesis is that heme-dependent TLR4 activation and macrophage polarization to the M1 phenotype mediate this phenomenon. Our novel findings showed that PWH with CWP have elevated M1-specific macrophage chemokines (ENA-78, GRO-α, and IP-10) in plasma. In vitro, hemin-induced polarization of M0 and M2 macrophages to the M1 phenotype with low β-endorphins was mitigated by treating cells with the TLR4 inhibitor, TAK-242. Similarly, in vivo phenylhydrazine hydrochloride (PHZ), an inducer of hemolysis, injected into C57Bl/6 mice increased the M1/M2 cell ratio and reduced β-endorphin levels. However, treating these animals with the heme-scavenging protein hemopexin (Hx) or TAK-242 reduced the M1/M2 ratio and increased β-endorphins. Furthermore, Hx attenuated heme-induced mechanical, heat, and cold hypersensitivity, while TAK-242 abrogated hypersensitivity to mechanical and heat stimuli. Overall, these results suggest that heme-mediated TLR4 activation and M1 polarization of macrophages correlate with impaired endogenous opioid homeostasis and hypersensitivity in people with HIV

High Heme and Low Heme Oxygenase-1 Are Associated with Mast Cell Activation/Degranulation in HIV-Induced Chronic Widespread Pain

An overwhelming number of people with HIV (PWH) experience chronic widespread pain (CWP) throughout their lifetimes. Previously, we demonstrated that PWH with CWP have increased hemolysis and attenuated heme oxygenase 1 (HO-1) levels. HO-1 degrades reactive, cell-free heme into antioxidants like biliverdin and carbon monoxide (CO). We found that high heme or low HO-1 caused hyperalgesia in animals, likely through multiple mechanisms. In this study, we hypothesized that high heme or low HO-1 caused mast cell activation/degranulation, resulting in the release of pain mediators like histamine and bradykinin. PWH who self-report CWP were recruited from the University of Alabama at Birmingham HIV clinic. Animal models included HO-1−/− mice and hemolytic mice, where C57BL/6 mice were injected intraperitoneally with phenylhydrazine hydrochloride (PHZ). Results demonstrated that plasma histamine and bradykinin were elevated in PWH with CWP. These pain mediators were also high in HO-1−/− mice and in hemolytic mice. Both in vivo and in vitro (RBL-2H3 mast cells), heme-induced mast cell degranulation was inhibited by treatment with CORM-A1, a CO donor. CORM-A1 also attenuated mechanical and thermal (cold) allodynia in hemolytic mice. Together, the data suggest that mast cell activation secondary to high heme or low HO-1 seen in cells and animals correlates with elevated plasma levels of heme, histamine, and bradykinin in PWH with CWP