Test-bed of a real time detection system for L/H and H/L transitions implemented with the ITMS platform

A basic requirement of the data acquisition systems used in long pulse fusion experiments is to detect events of interest in the acquired signals in real time. Developing such applications is usually a complex task, so it is necessary to develop a set of hardware and software tools that simplify their implementation. An example of these tools is the Intelligent Test and Measurement System (ITMS), which offers distributed data acquisition, distribution and real time processing capabilities with advanced, but easy to use, software tools that simplify application development and system setup. This paper presents the application of the ITMS platform to solve the problem of detecting L/H and H/L transitions in real time based on the use of efficient pattern recognition algorithms

Staple-based paper electrochemical platform for celiac disease diagnosis

A staple-based electrochemical platform is proposed for the first time as a simple and low-cost detection system for paper-based devices. The system, that incorporates small and disposable stainless-steel staples as electrodes (modified with carbon ink in the case of the working electrode) is combined with a paper strip and is carefully optimized with ferrocene carboxylic acid. As a proof-of-concept, it was employed for the enzymatic (HRP-based) immunoelectroanalytical detection of human tissue anti-transglutaminase (anti-tTG), biomarker for celiac disease diagnosis. The intensity of the current due to the electrochemical reduction of TMB (HRP substrate) was recorded chronoamperometrically at -0.2 V in different paper areas. A linear relationship between the current measured at 30 s and the logarithm of the concentration of anti-tTG in the range comprised between 3 and 100 U.mL-1 was obtained. Negative and positive controls produced expected values. Results demonstrated that the paper/staple-combined platform is very convenient for the detection of electroactive analytes and other compounds that can be determined indirectly in bioassays.Fil: Nanni, Paula Inés. Universidad de Oviedo; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; ArgentinaFil: Gonzalez Lopez, Andrea. Universidad de Oviedo; EspañaFil: Nuñez Bajo, Estefanía. Universidad de Oviedo; EspañaFil: Madrid, Rossana Elena. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Fernandez Abedul, M. Teresa. Universidad de Oviedo; Españ

Sugar Cane, Software and Fashion: Public-Private Collaboration in Argentina

The design and implementation of productive development policies (PDPs) pose challenges for policymakers, especially in emerging economies. Although the existence of pervasive market and coordination failures pave the way for policy intervention, adopted instruments have often been less successful than expected, since policymakers usually lack important pieces of information, they generally do not have access to rigorous evaluations of current and former programs and State capabilities are frequently weak. Moreover, coordination problems between different agencies and/or levels of government often exist. The emergence of rent seeking and free riding behaviours, agency captures and corruption episodes are other factors that explain the abovementioned outcome. The lack of resilience of public policies in the light of frequent staff changes in the State bureaucracy also hinders PDPs effectiveness.Public-Private Collaboration Schemes (PPCs) may help to alleviate some of the factors that lead to problems in the design, implementation and monitoring of PDPs, insofar they may facilitate information exchange and coordination among the different agents involved, address some government failures, generate more transparency and contribute to the resilience of those policies. PPC may range from informal consultations with the private sector to the full involvement of the latter in the policy design, execution and monitoring stages, including schemes such as the participation of the private sector in advisory and supervisory bodies, technological and productive alliances, deliberation councils, etc.The evidence gathered from the case studies will allow us to highlight common factors that may lead to the success (or failure) of PPCS, as well as idiosyncratic features related to the objectives, actors and/or specific arrangements adopted in each case. The result of this analysis is presented in the final section, where we distil lessons that may be applied to other sectors/regions in order to a better diffusion and effectiveness of PPC schemes.Fil: Bisang, Roberto Oscar. No especifíca;Fil: Gonzalez, Andrea. No especifíca;Fil: Hallak, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lopez, Andres Flavio. Centro de Investigaciones para la Transformación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ramos, Daniela. No especifíca;Fil: Rozemberg, Ricardo Daniel. No especifíca

Experimental study and calculation of the electron transfer coefficients on the dissolution behavior of chitosan in organic acids

Chitosan (CH) consists of water-insoluble N-acetylglucosamine and D-glucosamine molecules and has a higher solubility at a pH below six. This studyevaluated the solubility of chitosan in solutions of organic acids for the formation of films. HyperChemTMsoftware was used to perform the quantum analysis. In the experimental trials, the total soluble mass (TSM) and the viscosity of the solutions were measured by capillary viscometer. The chitosan filmswere made by the plate melting method, and the filmcharacteristics were evaluated. A quantum simulation suggested that lactic acid (LA) has a greater stability to react with chitosan. It was then verified experimentally that LA is a better solvent for chitosan due to the increase in its viscosity. The chemical interaction between CH and LA in solution favors the polymerization of films with better physical properties. We thereforeconclude that the uniformity in the formation of films of this polymer depends on the chemical interaction between the CH and the acid and not on the degree of solubility of the polymer

First record of the fish Dules auriga (perciformes, serranidae) in continental waters of Río Negro, General Conesa, Argentina, and description of its sagitta otolith

Dules auriga es una especie eurihalina, la única del género Dules, perteneciente a la familia Serranidae. Se distribuye desde Rio de Janeiro (Brasil) hasta Península de Valdés (Argentina). En este trabajo se describe por primera vez su hallazgo inusual en aguas del río negro, Río Negro, Argentina. El individuo juvenil fue capturado con caña operada desde la costa. Asimismo, se describió la morfología del otolito sagitta. Los otolitos fueron extraídos, fotografiados y descriptos. La sagitta tiene forma sagital, con borde dorsal sinuoso, borde ventral liso y un sulcus ostial bien delimitado. Este primer resultado morfológico aporta información al conocimiento de los Serranidae y de estas estructuras de importancia taxonómica en particular. Asimismo, la presencia inusual de este individuo en la zona de hallazgo requerirá mayor intensidad de muestreo para definir si su presencia es casual o se está estableciendo en la zona.Dules auriga is an eurihaline species, the only species in the genus Dules belonging to the Serranidae family. It is distributed from Rio de Janeiro (Brazil) to Península Valdés (Argentina). In this work describes for the first time its unusual find in the waters of the Negro river, Río Negro, Argentina. The juvenile individual was captured with a rod operated from the coast. Likewise, the morphology of the sagitta otolith was described. The otoliths were extracted, photographed, and described. Sagitta is sagittal in shape with a sinuous dorsal border, a smooth ventral border, and a well-defined ostial sulcus. This first morphological result contributes information to the knowledge of the Serranidae and of these structures of taxonomic importance in particular. T. Likewise, the unusual presence of this individual in the area of discovery will require greater intensity of sampling to define whether its presence is accidental or is establishing itself in the area.Fil: Gonzalez Dubox, María Cecilia. Universidad Nacional de Río Negro. Sede Atlántica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lopez Greco, Laura Susana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Tombari, Andrea Diana. Universidad Nacional de Río Negro. Sede Atlántica; Argentin

First record of the fish Dules auriga (perciformes, serranidae) in continental waters of Río Negro, General Conesa, Argentina, and description of its sagitta otolith

Dules auriga es una especie eurihalina, la única del género Dules, perteneciente a la familia Serranidae. Se distribuye desde Rio de Janeiro (Brasil) hasta Península de Valdés (Argentina). En este trabajo se describe por primera vez su hallazgo inusual en aguas del río negro, Río Negro, Argentina. El individuo juvenil fue capturado con caña operada desde la costa. Asimismo, se describió la morfología del otolito sagitta. Los otolitos fueron extraídos, fotografiados y descriptos. La sagitta tiene forma sagital, con borde dorsal sinuoso, borde ventral liso y un sulcus ostial bien delimitado. Este primer resultado morfológico aporta información al conocimiento de los Serranidae y de estas estructuras de importancia taxonómica en particular. Asimismo, la presencia inusual de este individuo en la zona de hallazgo requerirá mayor intensidad de muestreo para definir si su presencia es casual o se está estableciendo en la zona.Dules auriga is an eurihaline species, the only species in the genus Dules belonging to the Serranidae family. It is distributed from Rio de Janeiro (Brazil) to Península Valdés (Argentina). In this work describes for the first time its unusual find in the waters of the Negro river, Río Negro, Argentina. The juvenile individual was captured with a rod operated from the coast. Likewise, the morphology of the sagitta otolith was described. The otoliths were extracted, photographed, and described. Sagitta is sagittal in shape with a sinuous dorsal border, a smooth ventral border, and a well-defined ostial sulcus. This first morphological result contributes information to the knowledge of the Serranidae and of these structures of taxonomic importance in particular. T. Likewise, the unusual presence of this individual in the area of discovery will require greater intensity of sampling to define whether its presence is accidental or is establishing itself in the area.Fil: Gonzalez Dubox, María Cecilia. Universidad Nacional de Río Negro. Sede Atlántica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lopez Greco, Laura Susana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Tombari, Andrea Diana. Universidad Nacional de Río Negro. Sede Atlántica; Argentin

Electrochemical Detection for Isothermal Loop-Mediated Amplification of Pneumolysin Gene of Streptococcus pneumoniae Based on the Oxidation of Phenol Red Indicator

A highly sensitive electrochemical methodology for end-point detection of loop-mediated isothermal nucleic acid amplification reactions was developed. It is based on the oxidation process of phenol red (PR), commonly used as a visual indicator. The dependence of its redox process on pH, which changes during amplification, allows performing quantitative measurements. Thus, the change in the oxidation potential of PR during the amplification is used, for the first time, as the analytical signal that correlates with the number of initial DNA copies. As a proof-of-concept, the amplification of the pneumolysin gene from Streptococcus pneumoniae, one of the main pathogens causing community-acquired pneumonia, is performed. Combination of isothermal amplification with electrochemical detection, performed on small-size flexible electrodes, allows easy decentralization. Adaptation to the detection of other pathogens causing infectious diseases would be very useful in the prevention of future epidemics

Morphology of sagitta, lapillus and asteriscus of Patagonian Genidens barbus

Los otolitos son estructuras de carbonato de calcio, depositado en forma de aragonita, ubicados en el oído interno de peces óseos. En los últimos años se han convertido en una herramienta útil para la determinación de especies ícticas, estudios de edad, contenidos tróficos, identificación de stocks pesqueros. El objetivo del presente trabajo fue describir la morfología de los tres pares de otolitos del bagre marino Genidens barbus de distribución patagónica. Se analizaron los otolitos de ejemplares obtenidos en cuatro puntos de muestreo en el Río Negro. Se observó que los otolitos presentan el patrón morfológico general propuesto para Siluriformes, donde los lapilli son los más grandes y robustos de los tres. El lapillus presentó forma redondeada, elevación pronunciada en posición central, sulcus delimitado, pero poco evidente, sin evidencia de sulcus menor. La sagitta es claviforme, con región posterior cónica alargada, alas dorsales que se continúan como expansiones laterales denominadas alas basales y presencia de fisura. El asteriscus tiene forma auricular, abertura mediana, rostro corto aguzado, antirrostro poco desarrollado y fosa acústica poco curva. En este trabajo se describen por primera vez los otolitos sagitta y asteriscus para la especie G. barbus, completando así la descripción de los tres pares.Otoliths are calcium carbonate structures, deposited as aragonite, located in the inner ear of bony fish. In recent years, they have become a useful tool for the determination of fish species, trophic content, age studies, and the identification of fish stocks. The objective of this work was to describe the morphology of the three pairs of otoliths of the marine catfish Genidens barbus of Patagonian distribution. Otoliths of specimens obtained at four sampling points in the Negro River were analyzed. It was observed that otoliths presented the general morphological pattern proposed for Siluriformes, where lapilli are the largest and most robust of the three. Lapillus presented a rounded shape, a pronounced elevation in the central position, a delimited sulcus, but not very evident, without evidence of a minor sulcus. The sagitta is claviform, with an elongated conical posterior region, dorsal wings that continue as lateral expansions called basal wings, and the presence of a fissure. The asteriscus has an ear shape, a median opening, a short pointed rostrum, a poorly developed anti-rostrum and a slightly curved acoustic fossa. In this work, sagitta and asteriscus otoliths were described for the first time for the species G. barbus, thus completing the description of the three pairs.Fil: Gonzalez Dubox, María Cecilia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Rio Negro. Sede Choele Choel del Centro de Investigaciones y Transferencia de Rio Negro | Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Sede Choele Choel del Centro de Investigaciones y Transferencia de Rio Negro.; ArgentinaFil: Córdoba Gironde, Sofía. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Rio Negro. Sede Choele Choel del Centro de Investigaciones y Transferencia de Rio Negro | Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Sede Choele Choel del Centro de Investigaciones y Transferencia de Rio Negro.; ArgentinaFil: Lopez, Laura Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Tombari, Andrea Diana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Rio Negro. Sede Choele Choel del Centro de Investigaciones y Transferencia de Rio Negro | Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Sede Choele Choel del Centro de Investigaciones y Transferencia de Rio Negro.; Argentin