The expression patterns of genes involved in the RNAi pathways are tissue-dependent and differ in the germ and somatic cells of mouse testis

6 páginas, 3 figuras, 2 tablas -- PAGS nros. 306-311Different RNA interference (RNAi) components participate in post-transcriptional regulation via RNA silencing. The expression pattern of the genes Drosha and Dicer and the members of the Argonaute family Ago1, Ago2, Ago3 and Ago4, all elements participating in the RNAi pathways, were investigated in mouse somatic tissues and testis using quantitative RT-PCR. Expression patterns of different testis cells and those emerging during testis development were also investigated. The differential patterns of expression seen suggest potential pleiotropic roles for certain components of the RNAi machinery. Both spermatocytes and spermatids showed a defined gene expression pattern. The strong expression of Ago4 in germ cells suggests that this protein plays a key role in germ-cell differentiation in the seminiferous epitheliumThis work was supported by grants from the MEC (BFU2004-03977/BFI) and the MSC (FIS PI071007)Peer reviewe

ROV's Video Recordings as a Tool to Estimate Variation in Megabenthic Epifauna Diversity and Community Composition in the Guaymas Basin

Patterns in benthic megafauna diversity in littoral and intertidal zones in the Gulf of California have been associated with both habitat heterogeneity and substrate type. Current knowledge of invertebrate communities in hard bottom habitats at depths > 200 m in the Gulf is poor due to the methodological limitations inherent in sampling deep habitats. Using video imagery of benthic habitats coupled with environmental data from the Remotely Operated Vehicle Doc Ricketts, we documented variation in the diversity and community composition of the benthos from 849 to 990 m depth in the NW limit of the Guaymas Basin, in relation to dissolved oxygen and substrate characteristics. This depth range overlaps an oxygen minimum zone where oxygen drops to levels < 0.5 ml L-1 and strong gradients in a narrow depth range occur. Dissolved oxygen varied along our benthic survey from 0.200 to 0.135 ml L-1. We observed high taxonomic richness across an area of rocky outcrops through the lower transition zone. This megafaunal pattern differs from reports from other oxygen minimum zones characterized by a great abundance of a few species. Taxonomic richness diminished at depths with reduced dissolved oxygen in the lower boundary of the oxygen minimum zone with increasing soft sediment cover. We found that rocky outcrops and structure-forming organisms such as corals, sponges, and oyster aggregations supported a higher diversity (H' = 0.8) than soft sediment (H' = 0.7) as have been observed in other habitats such as seamounts. Environmental variables that explained most of the megafaunal variation were substrate type (18.4%), depth (1.14%) and temperature (0.9%). Salinity (0.45%) and dissolved oxygen (0.3%) were less important factors to explain the megafaunal composition variance. Substrate type played a key role in the diversity and composition of benthic megafauna. These results broaden our understanding concerning the potential roles of substrate characteristics in the community composition of the deep-sea benthic megafaunal assemblages in the Gulf of California and oxygen minimum zones in general

Confinement of electrons in layered metals

We analyze the out of plane hopping in models of layered systems where the in--plane properties deviate from Landau's theory of a Fermi liquid. We show that the hopping term acquires a non trivial energy dependence, due to the coupling to in plane excitations, and can be either relevant or irrelevant at low energies or temperatures. The latter is always the case if the Fermi level lies close to a saddle point in the dispersion relation.Comment: 4 pages, 1 eps figur