Toshiba GCS

El presente trabajo es un recabado de la experiencia del alumno Miguel González cursando el proyecto de aplicación profesional dentro de la empresa de Toshiba, dentro de este documento se verán las tareas que realizó, el proyecto del cual formó parte y las responsabilidades que traía la participación de este. Se verá expresado como a lo largo del curso fueron necesarias llevar a cabo diferentes tareas de aprendizaje donde no solamente se vea beneficiado la experiencia profesional, sino también se verá un crecimiento personal dentro del ámbito personal, humano y estudiantil. Las tareas y actividades que fueron realizadas durante este lapso del tiempo serán registradas para poder tener una mejor visualización de la evolución del alumno durante la estadía de becario. Estas tareas y actividades incluyen las de autoaprendizaje, entrega de productos y comunicación integrantes del equipo. Dado que este es el segundo periodo de becario se verá en aumento la cantidad de responsabilidades y tareas relacionadas con el proyecto, dichas responsabilidades pueden requerir investigación previa. Será presentado dentro de un plan de actividades y estudios relacionado con todos los requisitos para el correcto desarrollo del plan becario, dicho plan será planteado y explicado dentro de este documento. Cada apartado dentro del plan contendrá explicado el área que se trabajará y como se trabajará, así como con que actividad y proyecto estén relacionados. Al mismo tiempo será presentado el plan de comunicación que se utilizará para mandar mensajes y mandar entregables con el resto de los integrantes del equipo, dicho equipo será presentados junto con su rol dentro del proyecto. Para la correcta documentación de este proyecto se explicará el proyecto asignado, así como las tareas que conllevó. Además de esto se hará mención del equipo y el personal con el cual se realizó cada una de las tareas.ITESO, A.C

Toshiba GCS

El presente trabajo es un recabado de la experiencia del alumno Miguel González cursando el proyecto de aplicación profesional dentro de la empresa de Toshiba, dentro de este documento se verán las tareas que realizó, el proyecto del cual formó parte y las responsabilidades que traía la participación del mismo. Se verá expresado como a lo largo del curso fueron necesarias llevar a cabo tareas de aprendizaje donde no solamente se vea beneficiado la experiencia profesional, sino también se verá un crecimiento personal dentro del ámbito personal, humano y estudiantil. Las tareas y actividades que fueron realizadas durante este lapso del tiempo serán registradas para poder tener una mejor visualización de la evolución del alumno durante la estadía de becario. Estas tareas y actividades incluyen las de autoaprendizaje, entrega de productos y comunicación integrantes del equipo.ITESO, A.C

Isolation of Lactic Acid Bacteria (LAB) from Salmonids for Potential Use as Probiotics: In Vitro Assays and Toxicity Assessment of Salmo trutta Embryonated Eggs

This research investigates the potential of lactic acid bacteria (LAB) from freshwater salmonids as prospective probiotics for application in aquaculture. LAB and pathogenic bacteria were obtained from mucus and tissues of Oncorhynchus mykiss and Salmo trutta from fish farms in northeast Spain that had not used antibiotics for the six months preceding the study. Isolates were identified using Gram staining and sequencing of 16S rRNA and ITS-1. To assess the safety of the LAB, antibiotic susceptibility tests (ASTs) against 23 antimicrobials were performed. In vitro antagonism assays were conducted to evaluate the inhibitory effects of living LAB using the agar diffusion test method and their metabolites using the agar well diffusion method. The assays targeted six specific pathogens: Aeromonas salmonicida subsp. salmonicida, Carnobacterium maltaromaticum, Vagococcus salmoninarum, Yersinia ruckeri, Lactococcus garvieae, and the marine pathogen Vibrio jasicida. Additionally, a toxicity assay was conducted on embryonic eggs of S. trutta. The ASTs on probiotic LAB candidates revealed varied responses to antimicrobials, but no resistance to oxytetracycline or florfenicol, which are two antibiotics commonly used in aquaculture, was detected. The in vitro assays indicate that LAB exhibit antagonistic effects against pathogens, primarily when directly stimulated by their presence. In applications involving embryonic eggs or larvae, certain live strains of LAB were found to have adverse effects, with some isolates resulting in higher mortality rates compared to the control group or other isolates. Furthermore, the potential pathogenicity of certain LAB strains, typically considered safe in salmonids, warrants deeper investigation

Utilidad de una estrategia de cribado de hipertensión ocular y glaucoma en atención primaria

ObjetivosEvaluar la utilidad de una estrategia de cribado de glaucoma e hipertensión ocular (HTO) medida como número de casos detectados. Evaluar la aceptabilidad de la toma de presión intraocular (PIO) y la aparición de efectos secundarios.DiseñoEstudio descriptivo transversal.EmplazamientoCentro de salud urbano y consulta de oftalmología del hospital de referencia.ParticipantesEn total, 2.044 pacientes mayores de 40 años, seleccionados por muestreo consecutivo entre los que consultaron en el centro de salud durante 9 meses. Se excluyeron los sujetos diagnosticados de glaucoma, HTO, conjuntivitis o enfermedad corneal.IntervencionesToma de PIO con Tonopen XL en atención primaria. Se remitió a oftalmología a los sujetos con una PIO≥21 mmHg. En éstos se midió la PIO con la prueba de Goldmann y, en los que se confirmó la HTO, se realizaron una oftalmoscopia y una campimetría.Mediciones principalesPorcentaje de sujetos con glaucoma, sospecha de glaucoma e HTO confirmada en oftalmología. Valor predictivo positivo (VPP) para HTO.ResultadosSe detectaron 100 sujetos con HTO (4,89%; intervalo de confianza [IC] del 95%, 3,93-5,85%), de los que 21 fueron diagnosticados de glaucoma (1,04%; IC del 95%, 0,57-1,49%) y 10 de sospecha de glaucoma (0,49%; IC del 95%, 0,16-0,82). El VPP para HTO fue del 44,27%. La aceptabilidad de la prueba fue del 98,09%. Ningún paciente presentó efectos secundarios tras la toma de la PIO.ConclusionesLa estrategia evaluada es útil en cuanto al porcentaje de sujetos con glaucoma e HTO detectados. La aceptabilidad de la toma de la PIO con Tonopen XL es alta.ObjectivesTo evaluate the usefulness of a glaucoma and intraocular hypertension screening strategy for new cases detected. To evaluate the acceptability of taking intraocular pressure (IOP) and the appearance of side effects.DesignCross-sectional, descriptive study.SettingAn urban health centre and the ophthalmology clinic of its main hospital.ParticipantsA total of 2044 patients aged over 40, 63.5% women and 36.5% men, with a mean age of 61.23 (SD, 11.42). They were selected by consecutive sampling from patients who visited the health centre over a 9-month period. Subjects diagnosed with glaucoma, ocular hypertension (OH), conjunctivitis, or corneal pathology were excluded.InterventionsTaking of IOP with Tonopen XL in primary care. Subjects with IOP ≥21 mm Hg were referred to ophthalmology. In these patients, IOP was measured with Goldmann, and patients with confirmed OH received ophthalmoscopy and campimetry.Main measurementsPercentage of subjects with glaucoma, suspected glaucoma, and OH confirmed in ophthalmology. Positive predictive value (PPV) for OH.ResultsOne hundred subjects with OH were detected (4.89%; 95% CI, 3.93%-5.85%), of whom 21 were diagnosed with glaucoma (1.04%; 95% CI, 0.57-1.49) and 10 with suspected glaucoma (0.49%; 95% CI, 0.16-0.82). The PPV for OH was 44.27%. The acceptability of the test was 98.09%. No patients presented with side-effects following the taking of their IOP.ConclusionsThe strategy evaluated is useful in terms of the number of subjects with glaucoma and OH detected. The acceptability of taking IOP with Tonopen XL was high

Investigation of EBT3 radiochromic film’s response to humidity

Purpose: The aim of this work is to investigate the effects of immersing EBT3 radiochromic film in water and to evaluate its contribution to the total uncertainty in dose determination. Materials and methods: We used 3 cm 9 3 cm EBT3 radiochromic films irradiated in the range of 0–70 Gy to study the impact of water immersion on the change in net optical density. These films were placed in a water container for a period of 24 h. The net optical density was measured before (0 h) and after of the immersion in water (1, 3, 6, 12, 18, and 24 h). The absorbance spectrum of the EBT3 radiochromic film was measured at 0 h and 24 h after immersion in water. The uncertainty in dose determination due to the effects of keeping the EBT3 radiochromic film submerged in water at 0, 1, and 24 h were recorded in the red, green, and blue channels. Results: We observed an increase in the net optical density as an effect on the film due to its immersion in water. The penetration of the water at the edges of the radiochromic film was observed to be a function of time during which the film remained in the water. On the other hand, the penetration of water at the edges of the film was found to be independent of irradiation dose. Conclusions: EBT3 radiochromic film is found more resistant to water penetration through the edges than its predecessors. However, there is evidence that suggest that liquid water damage the Nylon cover layer of the film by changing its optical properties. Therefore, it is recommended to build a new calibration curve for radiochromic films for a specific situation involving dose measurements in liquid water.UAEMex INN

Intrahepatic injection of adenovirus reduces inflammation and increases gene transfer and therapeutic effect in mice

Recombinant adenoviruses (Ad) are among the most extensively used vectors for liver gene transfer. One of the major limitations for the clinical application of these vectors is the inflammatory immune response associated with systemic administration of high dose of virus. We evaluated the effect of Ad administration route on the inflammatory immune response and liver transgene expression. We compared direct intrahepatic injection (IH) with the systemic administration via tail vein (IV). IH injection of Ad resulted in a lower inflammatory response and a higher transgene expression. When a relatively low dose of virus was used, IV administration resulted in no detectable protein expression but production of proinflammatory cytokines. In contrast, IH administration induced high levels of transgene expression and no inflammation, although we detected a transient hypertransaminemia, which fully resolved within days. Furthermore, IH injection resulted in a faster protein expression being more intense at the site of injection, whereas IV administration caused slower but diffuse liver expression. IH injection also reduced the spreading of the virus to other organs. Independently of the route, depletion of Kupffer cells significantly enhanced the transduction efficiency of Ad. This effect was stronger when using IV injection, indicating that IH injection partially overcomes Kupffer cell phagocytic activity. Moreover, the antitumor efficacy of high-capacity-Ad encoding murine interleukin-12 (IL-12) was significantly greater when the vector was administered by IH injection than when given IV. In conclusion, IH injection of adenovirus represents a safe and efficient administration route for clinical applications of gene therapy targeting the liver

Análisis de los rayos registrados en Canarias en el periodo 2006-2015

En zonas dominadas por centros de altas presiones semipermanentes, como Canarias, el aire descendente, los bajos niveles de humedad por encima de la inversión térmica y la estratificación estable, inhiben las tormentas, pero no completamente. A partir de los rayos registrados en una amplia zona geográfica que engloba las islas Canarias y Madeira y el área oceánica circundante, se analiza la distribución espacial y temporal de los mismos, estimando su densidad y el número de días de tormenta en rejillas de resolución 0,1°, y comparando sus características con las de otras zonas geográficas similares. Seguidamente, se evalúa el comportamiento de algunos parámetros eléctricos de los rayos, como la intensidad, la polaridad y la multiplicidad. A continuación, se trazan algunos rasgos que, con una serie más dilatada, pueden llegar a adquirir rango climatológico. Finalmente, se conjeturan algunas de las claves del peculiar episodio de agosto de 2015

Aquacultural Homoeopathy: A Focus on Marine Species

Homoeopathy is an alternative medical system proposed by Samuel Hahnemann in the eighteenth century. It uses highly diluted and agitated substances that derived from plants, minerals or animals, which have shown to be effective in human medicine, agronomy, veterinary, and as a novelty, in marine aquaculture. Aquacultural homoeopathy has developed rapidly in recent years, partially motivated by the misuse of powerful drugs (hormones, antibiotics, disinfectants) that when solving a problem generate undesirable side effects. In the last 10 years, scientific articles have been published on its application in freshwater fish native to Brazil, obtaining beneficial effects on growth, survival, hepatosomatic index, development of muscle fibres and lipid content in muscle. At Centro de Investigaciones Biológicas del Noroeste (CIBNOR, Mexico: www.cibnor.mx), we have studied the effects of homoeopathy to improve the culture of economically important marine species of molluscs, fish and shrimp. In this chapter, we show a selection of different research with preliminary or advanced results, related to the use of homoeopathy and its impact on zootechnic, biochemical, genomic and transcriptomic parameters in marine molluscs, fish and crustaceans. The results obtained suggest that homoeopathy is an eco-friendly alternative applicable in aquaculture industry to improve various productive and health aspects