C-FAR - Carbon footprinting of archaeological research: Data collection methodology and interim report

Carbon Footprinting of Archaeology Research (C-FAR) focused on developing a method of determining the carbon footprint of university-led archaeological training expeditions.British Academ

Analysis and characterisation of human chorionic gonadotropin glycoforms in pregnancy and trophoblastic disorders

Human chorionic gonadotropin (hCG) is a heterogeneous glycoprotein hormone with a varying degree of carbohydrate moieties. Its glycosylation arrangements are increasingly recognized as a common and important element of disease pathophysiology and are associated with many disorders including gestational trophoblastic diseases (GTDs). This study aimed to optimise methodologies to permit the characterisation of hCG N-linked glycans from urine samples collected throughout normal pregnancy and GTD using matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF MS). hCG isolated from pooled pregnancy urine was used in this study. All the stages in pregnancy urine preparation were optimised; including conditions for hCG immunopurification, deglycosylation, solid-phase extraction of resulting glycan:protein mixture and application of N-glycans for MALDI-TOF MS analysis. GlycoQuest software was used to characterise specific N-glycans configurations from the resulting MALDI-TOF MS spectra. This methodology was then applied to urine samples collected throughout normal singleton pregnancy and urine collected from patients with choriocarcinoma. The relative abundance of glycans of different molecular weights and specific types (i.e. fucosylated, sialylated, bisected and sulphated) at each stage of normal pregnancy and in GTD were compared. Each stage of optimisation increased the number of N-glycans detected such that we were ultimately able to detect 50 different glycans in normal pregnancy urine. In these samples, advancing gestation was associated with an increase in the proportion of branched N-glycans and multi-fucosylated N-glycans. Also, a significant increase in the proportion of high molecular weight glycans (>2100 Da) between choriocarcinoma and first-trimester normal pregnancy was observed. Further striking differences in the repertoire of glycan expression were also seen in choriocarcinoma urine compared with first-trimester pregnancy urine. The proportion of multi-fucosylated and tri-and tetra antennal, glycans was increased 3 and 2 fold respectively. In addition, 14 unique N-linked glycan structures were identified in choriocarcinoma samples. These included hyperfucosylated (7 fucose groups) and hypersialylated (4 sialic acid groups) glycans. A feature of this unique set of glycans was that they contained a combination of multiple branching, fucosylation, sialylation, sulphation and glycans with Lewis X terminal epitopes. In summary, we have successfully developed a methodology for the detection of a diverse range of N-linked glycans from hCG. These results suggest that this approach can be successfully used for the detection of novel glyco-biomarkers for the early detection of choriocarcinoma and may be applied to other GTDs associated with a dysregulation of hCG expression

On the Solution Space of Differentially Rotating Neutron Stars in General Relativity

A highly accurate, multi-domain spectral code is used in order to construct sequences of general relativistic, differentially rotating neutron stars in axisymmetry and stationarity. For bodies with a spheroidal topology and a homogeneous or an N=1 polytropic equation of state, we investigate the solution space corresponding to broad ranges of degree of differential rotation and stellar densities. In particular, starting from static and spherical configurations, we analyse the changes of the corresponding surface shapes as the rate of rotation is increased. For a sufficiently weak degree of differential rotation, the sequences terminate at a mass-shedding limit, while for moderate and strong rates of differential rotation, they exhibit a continuous parametric transition to a regime of toroidal fluid bodies. In this article, we concentrate on the appearance of this transition, analyse in detail its occurrence and show its relevance for the calculation of astrophysical sequences. Moreover, we find that the solution space contains various types of spheroidal configurations, which were not considered in previous work, mainly due to numerical limitations.Comment: 9 pages, 10 figures, version to be published in MNRAS ; no major changes with respect to v1: title, abstract and other things were modified to put more emphasis on general aspects of the wor

Konzeptualisierung der Emotionen auf der Mikrotextebene (am Beispiel von Sprichwörtern mit der Komponente Zorn)

In this paper I would like to present the emotionality and emotions in German proverbs on the example of proverbs with the component anger. I am focused not on theoretical considerations about the proverb but on the analysis of the collected language material. The component anger is one of the highly productive proverbial components, it has been found in over four hundred units. It should be noted that most of the proverbs feature the negative effects of this emotion. The analysis of the material allowed for the extraction of nine main groups which show how anger is perceived and described in German proverbs. For example: anger blinds people, makes them lose self-control and brings about serious consequences. Although currently proverbs are often negatively labelled as simple wisdom of ordinary people, I think that they have not entirely lost their social and educational content