Ascertainment of surfactin concentration in bubbles and foam column operated in semi-batch

This paper describes a mathematical model for the convection, diffusion, and balance phenomena for predicting the depletion curves, i.e., variations in the timed surface-active molecule concentration for fractionation processes in bubbles and foam column, operated in semi-batch. The model was applied for the purification of the surfactin solution and the results were compared with experimental data. Gibbs adsorption curves were obtained for the biosurfactant at different temperatures, and then adjusted with estimated parameters, according to the Langmuir adsorption model. The gas bubble sizes were optically determined. The isotherm adsorption parameters and bubble average diameter are crucial for the attainment of the depletion curves, generated by the model described. The results demonstrate that the process is most effective when operating a column with reduced gas flow and low initial concentration. A top product with two or thirty times greater concentration than the initial one was achieved and the highest biosurfactant concentrations were attained for higher operating temperatures. It was also observed that bubble diameter increased with a higher gas flow. The adjustment obtained for the adsorption curves of Gibbs was satisfactory. Therefore, there was evidence that surfactin molecules adsorb in monolayers in the liquid-gas interface.73COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESSem informaçã

Modeling malaria infection and immunity against variant surface antigens in Príncipe Island, West Africa

After remarkable success of vector control campaigns worldwide, concerns about loss of immunity against Plasmodium falciparum due to lack of exposure to the parasite are relevant since an increase of severe cases in less immune individuals is expected. We present a mathematical model to investigate the impact of reducing exposure to the parasite on the immune repertoire against P. falciparum erythrocyte membrane protein 1 (PfEMP1) variants. The model was parameterized with data from Príncipe Island, West Africa, and applied to simulate two alternative transmission scenarios: one where control measures are continued to eventually drive the system to elimination; and another where the effort is interrupted after 6 years of its initiation and the system returns to the initial transmission potential. Population dynamics of parasite prevalence predict that in a few years infection levels return to the pre-control values, while the re-acquisition of the immune repertoire against PfEMP1 is slower, creating a window for increased severity. The model illustrates the consequences of loss of immune repertoire against PfEMP1 in a given setting and can be applied to other regions where similar data may be available

Interação da temperatura com o ácaro parasita Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) sobre o desenvolvimento de Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae)

O presente trabalho teve como objetivo avaliar o efeito da interação de diferentes temperaturas com o parasita Acarophenax lacunatus (Cross & Krantz) na supressão populacional de Rhyzopertha dominica (Fabricius). Os ensaios foram realizados com e sem o parasita, em cinco repetições, às temperaturas de 20, 25, 30 e 35ºC, umidade relativa de 60 ± 5% e escotofase de 24h. As unidades experimentais consistiram de placas de Petri, contendo 40 g de grãos de trigo (13% b.u.), infestados com 10 adultos de R. dominica. Foram realizadas três inoculações de cinco fêmeas de A. lacunatus por placa de Petri. As avaliações foram realizadas depois de 20, 40, 60, 80, 100 e 120 dias do início do experimento. A interação da temperatura com A. lacunatus mostrou ser uma importante ferramenta para a supressão populacional de R. dominica. Todavia, em temperaturas maiores que 25ºC, mesmo A. lacunatus reduzindo grande proporção das fases imaturas de R. dominica, as perdas de massa dos grãos foram elevadas aos 120 dias do armazenamento. A manutenção da temperatura dos grãos armazenados a 20ºC pode ser usada como complemento para o controle biológico de R. dominica com A. lacunatus.The objective of this study was to evaluate the effect of different temperatures with the association of the mite Acarophenax lacunatus (Cross & Krantz) on the population suppression of Rhyzopertha dominica (Fabricius). The experimental units were petri dishes containing 40 g of whole wheat grains (13% moisture content) infested with 10 adults of R. dominica, under the temperatures of 20, 25, 30 and 35ºC, with or without A. lacunatus, in five replicates. Relative humidity of 60 ± 5% and escotophase of 24h were used for all temperatures. Five days after the infestation, five mites were inoculated in each experimental unit. The evaluations were carried out at 20, 40, 60, 80, 100 and 120 days after R. dominica infestation. The interaction of the temperature with the parasite A. lacunatus was an important tool for the population suppression of R. dominica. In temperatures higher than 25ºC, however, despite the reduction of the immature stages of R. dominica, there was a high grain weight loss after 120 days. The maintenance of the temperature of the wheat grains stored at 20ºC can be used to complement the biological control of R. dominica with A. lacunatus

Prevalence and predictors of coronary artery disease in patients with a calcium score of zero

The absence of coronary calcification is associated with an excellent prognosis. However, a calcium score of zero does not exclude the presence of coronary artery disease (CAD) or the possibility of future cardiovascular events. Our aim was to study the prevalence and predictors of coronary artery disease in patients with a calcium score of zero. Prospective registry consisted of 3,012 consecutive patients that underwent cardiac CT (dual source CT). Stable patients referred for evaluation of possible CAD that had a calcium score of zero (n = 864) were selected for this analysis. The variables that were statistically significant were included in a multivariable logistic regression model. From 864 patients with a calcium score of zero, 107 (12.4 %) had coronary plaques on the contrast CT (10.8 %, n = 93 with nonobstructive CAD and 1.6 %, n = 14 with obstructive CAD). By logistic regression analysis, the independent predictors of CAD in this population were age >55 years [odds ratio (OR) 1.63 (1.05-2.52)], hypertension [OR 1.64 (1.05-2.56)] and dyslipidemia [OR 1.54 (1.00-2.36)]. In the presence of these 3 variables, the probability of having coronary plaques was 21 %. The absence of coronary artery calcification does not exclude the presence of coron

Diabetes as an independent predictor of high atherosclerotic burden assessed by coronary computed tomography angiography: The coronary artery disease equivalent revisited

(1) To study the prevalence and severity of coronary artery disease (CAD) in diabetic patients. (2) To provide a detailed characterization of the coronary atherosclerotic burden, including the localization, degree of stenosis and plaque composition by coronary computed tomography angiography (CCTA). Single center prospective registry including a total of 581 consecutive stable patients (April 2011-March 2012) undergoing CCTA (Dual-source CT) for the evaluation of suspected CAD without previous myocardial infarction or revascularization procedures. Different coronary plaque burden indexes and plaque type and distribution patterns were compared between patients with (n = 85) and without diabetes (n = 496). The prevalence of CAD (any plaque; 74.1 vs. 56 %; p = 0.002) and obstructive CAD (≥50 % stenosis; 31.8 vs. 10.3 %; p<0.001) were significantly higher in diabetic patients. The remaining coronary atherosclerotic burden indexes evaluated (plaque in LM-3v-2v with prox. LAD; SIS; SSS; CT-LeSc) were also significantly higher in diabetic patients. In the per segment analysis, diabetics had a higher percentage of segments with plaque in every vessel (2.6/13.1/7.5/10.5 % for diabetics vs. 1.4/7.1/3.3/4.4 % for nondiabetics for LM, LAD, LCx, RCA respectively; p<0.001 for all) and of both calcified (19.3 vs. 9.2 %, p<0.001) and noncalcified or mixed types (14.4 vs. 7.0 %; p<0.001); the ratio of proximal-to-distal relative plaque distribution (calculated as LM/proximal vs. mid/distal/branches) was lower for diabetics (0.75 vs. 1.04; p = 0.009). Diabetes was an independent predictor of CAD and was also associated with more advanced CAD, evaluated by indexes of coronary atherosclerotic burden. Diabetics had a significantly higher prevalence of plaques in every anatomical subset and for the different plaque composition. In this report, the relative geographic distribution of the plaques within each subgroup, favored a more mid-to-distal localization in the diabetic patients