191 research outputs found
Rapid Transcriptional Reprogramming Triggered by Alteration of the Carbon/Nitrogen Balance Has an Impact on Energy Metabolism in Nostoc sp. PCC 7120
Nostoc (Anabaena) sp. PCC 7120 is a filamentous cyanobacterial species that fixes N2
to nitrogenous compounds using specialised heterocyst cells. Changes in
the intracellular ratio of carbon to nitrogen (C/N balance) is known to
trigger major transcriptional reprogramming of the cell, including
initiating the differentiation of vegetative cells to heterocysts.
Substantial transcriptional analysis has been performed on Nostoc
sp. PCC 7120 during N stepdown (low to high C/N), but not during C
stepdown (high to low C/N). In the current study, we shifted the
metabolic balance of Nostoc sp. PCC 7120 cultures grown at 3% CO2 by introducing them to atmospheric conditions containing 0.04% CO2
for 1 h, after which the changes in gene expression were measured using
RNAseq transcriptomics. This analysis revealed strong upregulation of
carbon uptake, while nitrogen uptake and metabolism and early stages of
heterocyst development were downregulated in response to the shift to
low CO2. Furthermore, gene expression changes revealed a
decrease in photosynthetic electron transport and increased
photoprotection and reactive oxygen metabolism, as well a decrease in
iron uptake and metabolism. Differential gene expression was largely
attributed to change in the abundances of the metabolites
2-phosphoglycolate and 2-oxoglutarate, which signal a rapid shift from
fluent photoassimilation to glycolytic metabolism of carbon after
transition to low CO2. This work shows that the C/N balance in Nostoc
sp. PCC 7120 rapidly adjusts the metabolic strategy through
transcriptional reprogramming, enabling survival in the fluctuating
environment.</p
Numerical study of the effect of wall temperature profiles on the premixed methane–air flame dynamics in a narrow channel
Time-accurate simulations of premixed CH/air flame in a narrow, heated channel are performed using the DRM-19 reaction mechanism. The effect of different wall temperature profiles on the flame dynamics is investigated for three different inflow velocity conditions. At a low inflow velocity of 0.2 m s, the flame shows instabilities in the form of spatial oscillations and even flame extinction. With the increase of the inflow velocity, flames are prone to showing more stability at a medium inflow velocity of 0.4 m s, and eventually show flame stabilisation at a high inflow velocity condition of 0.8 m s for all the wall temperature profiles examined. The total chemical heat release rate and total gas-solid heat exchange rate are found to have a combined effect on the flame propagation speed that determines flame behaviours. Since the flame behaviours in terms of the oscillation frequency and amplitude for spatially oscillating flames, or the stream-wise stabilisation location for steady-state flames, are very sensitive to the chosen wall temperature profile, a "real" conjugate heat transfer model is recommended in order to capture all of the relevant combustion physics accurately
The small Ca 2+ -binding protein CSE links Ca 2+ signalling with nitrogen metabolism and filament integrity in Anabaena sp. PCC 7120
Funder: FP7 People: Marie-Curie Actions; doi: http://dx.doi.org/10.13039/100011264; Grant(s): 2013–607607Abstract: Background: Filamentous cyanobacteria represent model organisms for investigating multicellularity. For many species, nitrogen-fixing heterocysts are formed from photosynthetic vegetative cells under nitrogen limitation. Intracellular Ca2+ has been implicated in the highly regulated process of heterocyst differentiation but its role remains unclear. Ca2+ is known to operate more broadly in metabolic signalling in cyanobacteria, although the signalling mechanisms are virtually unknown. A Ca2+-binding protein called the Ca2+ Sensor EF-hand (CSE) is found almost exclusively in filamentous cyanobacteria. Expression of asr1131 encoding the CSE protein in Anabaena sp. PCC 7120 was strongly induced by low CO2 conditions, and rapidly downregulated during nitrogen step-down. A previous study suggests a role for CSE and Ca2+ in regulation of photosynthetic activity in response to changes in carbon and nitrogen availability. Results: In the current study, a mutant Anabaena sp. PCC 7120 strain lacking asr1131 (Δcse) was highly prone to filament fragmentation, leading to a striking phenotype of very short filaments and poor growth under nitrogen-depleted conditions. Transcriptomics analysis under nitrogen-replete conditions revealed that genes involved in heterocyst differentiation and function were downregulated in Δcse, while heterocyst inhibitors were upregulated, compared to the wild-type. Conclusions: These results indicate that CSE is required for filament integrity and for proper differentiation and function of heterocysts upon changes in the cellular carbon/nitrogen balance. A role for CSE in transmitting Ca2+ signals during the first response to changes in metabolic homeostasis is discussed
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The small Ca 2+ -binding protein CSE links Ca 2+ signalling with nitrogen metabolism and filament integrity in Anabaena sp. PCC 7120
Funder: FP7 People: Marie-Curie Actions; doi: http://dx.doi.org/10.13039/100011264; Grant(s): 2013–607607Abstract: Background: Filamentous cyanobacteria represent model organisms for investigating multicellularity. For many species, nitrogen-fixing heterocysts are formed from photosynthetic vegetative cells under nitrogen limitation. Intracellular Ca2+ has been implicated in the highly regulated process of heterocyst differentiation but its role remains unclear. Ca2+ is known to operate more broadly in metabolic signalling in cyanobacteria, although the signalling mechanisms are virtually unknown. A Ca2+-binding protein called the Ca2+ Sensor EF-hand (CSE) is found almost exclusively in filamentous cyanobacteria. Expression of asr1131 encoding the CSE protein in Anabaena sp. PCC 7120 was strongly induced by low CO2 conditions, and rapidly downregulated during nitrogen step-down. A previous study suggests a role for CSE and Ca2+ in regulation of photosynthetic activity in response to changes in carbon and nitrogen availability. Results: In the current study, a mutant Anabaena sp. PCC 7120 strain lacking asr1131 (Δcse) was highly prone to filament fragmentation, leading to a striking phenotype of very short filaments and poor growth under nitrogen-depleted conditions. Transcriptomics analysis under nitrogen-replete conditions revealed that genes involved in heterocyst differentiation and function were downregulated in Δcse, while heterocyst inhibitors were upregulated, compared to the wild-type. Conclusions: These results indicate that CSE is required for filament integrity and for proper differentiation and function of heterocysts upon changes in the cellular carbon/nitrogen balance. A role for CSE in transmitting Ca2+ signals during the first response to changes in metabolic homeostasis is discussed
On the influence of modelling choices on combustion in narrow channels
This paper examines the effect of modelling choices on the numerical simulation of premixed methane/air combustion in narrow channels. Knowledge on standard and well-accepted numerical methods in literature are collected in a cohesive document. The less well-established modelling choices have been thoroughly evaluated and discussed. A systematic method of computing the grid convergence index (GCI) has been presented for refining the computational grid. Two types of inflow boundary conditions have been tested and compared in terms of their wave-damping characteristics. The effect of different reaction schemes on simulation results have been examined and an appropriate mechanism (DRM-19) has been selected. Various types of ignition strategies to initiate the flame have been tested and compared. The transient ignition process which has not been discussed extensively in existing literature has been quantitatively described in this paper
The small Ca2+-binding protein CSE links Ca2+ signalling with nitrogen metabolism and filament integrity in Anabaena sp. PCC 7120
Background Filamentous cyanobacteria represent model organisms for investigating multicellularity. For many species, nitrogen-fixing heterocysts are formed from photosynthetic vegetative cells under nitrogen limitation. Intracellular Ca2+ has been implicated in the highly regulated process of heterocyst differentiation but its role remains unclear. Ca2+ is known to operate more broadly in metabolic signalling in cyanobacteria, although the signalling mechanisms are virtually unknown. A Ca2+-binding protein called the Ca2+ Sensor EF-hand (CSE) is found almost exclusively in filamentous cyanobacteria. Expression of asr1131 encoding the CSE protein in Anabaena sp. PCC 7120 was strongly induced by low CO2 conditions, and rapidly downregulated during nitrogen step-down. A previous study suggests a role for CSE and Ca2+ in regulation of photosynthetic activity in response to changes in carbon and nitrogen availability. Results In the current study, a mutant Anabaena sp. PCC 7120 strain lacking asr1131 (Delta cse) was highly prone to filament fragmentation, leading to a striking phenotype of very short filaments and poor growth under nitrogen-depleted conditions. Transcriptomics analysis under nitrogen-replete conditions revealed that genes involved in heterocyst differentiation and function were downregulated in Delta cse, while heterocyst inhibitors were upregulated, compared to the wild-type.Conclusions These results indicate that CSE is required for filament integrity and for proper differentiation and function of heterocysts upon changes in the cellular carbon/nitrogen balance. A role for CSE in transmitting Ca2+ signals during the first response to changes in metabolic homeostasis is discussed.</div
Photosystem I Inhibition, Protection and Signalling: Knowns and Unknowns
Photosynthesis is the process that harnesses, converts and stores light energy in the form of chemical energy in bonds of organic compounds. Oxygenic photosynthetic organisms (i.e., plants, algae and cyanobacteria) employ an efficient apparatus to split water and transport electrons to high-energy electron acceptors. The photosynthetic system must be finely balanced between energy harvesting and energy utilisation, in order to limit generation of dangerous compounds that can damage the integrity of cells. Insight into how the photosynthetic components are protected, regulated, damaged, and repaired during changing environmental conditions is crucial for improving photosynthetic efficiency in crop species. Photosystem I (PSI) is an integral component of the photosynthetic system located at the juncture between energy-harnessing and energy consumption through metabolism. Although the main site of photoinhibition is the photosystem II (PSII), PSI is also known to be inactivated by photosynthetic energy imbalance, with slower reactivation compared to PSII; however, several outstanding questions remain about the mechanisms of damage and repair, and about the impact of PSI photoinhibition on signalling and metabolism. In this review, we address the knowns and unknowns about PSI activity, inhibition, protection, and repair in plants. We also discuss the role of PSI in retrograde signalling pathways and highlight putative signals triggered by the functional status of the PSI pool
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