A phase I study of PankoMab-GEX, a humanised glyco-optimised monoclonal antibody to a novel tumour-specific MUC1 glycopeptide epitope in patients with advanced carcinomas

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Shared pooled mobility: expert review from nine disciplines and implications for an emerging transdisciplinary research agenda

Shared pooled mobility has been hailed as a sustainable mobility solution that uses digital innovation to efficiently bundle rides. Multiple disciplines have started investigating and analyzing shared pooled mobility systems. However, there is a lack of cross-community communication making it hard to build upon knowledge from other fields or know which open questions may be of interest to other fields. Here, we identify and review 9 perspectives: transdisciplinary social sciences, social physics, transport simulations, urban and energy economics, psychology, climate change solutions, and the Global South research and provide a common terminology. We identify more than 25 000 papers, with more than 100 fold variation in terms of literature count between research perspectives. Our review demonstrates the intellectual attractivity of this as a novel perceived mode of transportation, but also highlights that real world economics may limit its viability, if not supported with concordant incentives and regulation. We then sketch out cross-disciplinary open questions centered around (1) optimal configuration of ride-pooling systems, (2) empirical studies, and (3) market drivers and implications for the economics of ride-pooling. We call for researchers of different disciplines to actively exchange results and views to advance a transdisciplinary research agenda

EEMlab: A graphical user-friendly interface for fluorimetry experiments based on the drEEM toolbox

[EN] Fluorescence has been widely employed for the characterization of organic matter. In particular, excitation emission matrixes (EEM) provide important qualitative information on its composition. However, the application of this technique is limited by the mathematical complexity involved, which requires the use of PARAFAC for deconvolution of the EEM in their components. To overcome the numerical problem specific MATLAB toolboxes for the PARAFAC deconvolution have been implemented (e.g. drEEM). This toolbox is widely used by the scientific community but its intrinsic complexity in terms of programming knowledge makes it difficult to use. In this regard and in order to facilitate the first approximation to the PARAFAC programming problem, this paper describes and offers to the community the EEMlab software application: a graphical user-firendly interface for fluorimetry experiments based on the drEEM toolbox. The interface is developed in order to facilitate not only the intuitive use of the drEEM (no previous MATLAB knowledge is needed) but also to automate many repetitive tasks (as the data load or the modeling loop) or even to manage the different formats of files being produced by all the devices involved in the process. In order to validate the EEMlab, the same experiment documented by the drEEM is reproduced. In addition, the EEMlab is tested again with conducting a new fluorimetry experiment and the results are presented at the end of the paper. Finally to appoint a reference to the public web site pabmitor.webs.upv.es/eemlab in where all the components of the EEMlab GUI (software, tutorial and datasets) are publicly available to the readers.The authors want to thank the financial support of the Generalitat Valenciana, Conselleria d'Educacio, Cultura i Esport (Spain) [GV/2015/074]. The authors want to thank the financial support of Ministerio de Educacion y Ciencia (Spain)(CTQ2015-69832-C4-4-R). Sara Garcia-Ballesteros would like to thank Ministerio de Economia y Competitividad (Spain) for her fellowship (BES-2013-066201). The authors want to thank Dr. F.S. García Einschlag who has independently tested the EEMlab and helped the authors to improve and validate the final version of the application.Micó Tormos, P.; García-Ballesteros, S.; Mora Carbonell, M.; Vicente Candela, R.; Amat Payá, AM.; Arqués Sanz, A. (2019). EEMlab: A graphical user-friendly interface for fluorimetry experiments based on the drEEM toolbox. Chemometrics and Intelligent Laboratory Systems. 188:6-13. https://doi.org/10.1016/j.chemolab.2019.03.001S61318

Modification of EGF-Like Module 1 of Thrombospondin-1, an Animal Extracellular Protein, by O-Linked N-Acetylglucosamine

Thrombospondin-1 (TSP-1) is known to be subject to three unusual carbohydrate modifications: C-mannosylation, O-fucosylation, and O-glucosylation. We now describe a fourth: O-β-N-acetylglucosaminylation. Previously, O-β-N-acetylglucosamine (O-β-GlcNAc) was found on a threonine in the loop between the fifth and sixth cysteines of the 20th epidermal growth factor (EGF)-like module of Drosophila Notch. A BLAST search based on the Drosophila Notch loop sequence identified a number of human EGF-like modules that contain a similar sequence, including EGF-like module 1 of TSP-1 and its homolog, TSP-2. TSP-1, which has a potentially modifiable serine in the loop, reacted in immuno-blots with the CTD110.6 anti-O-GlcNAc antibody. Antibody reactivity was diminished by treatment of TSP-1 with β-N-acetylhexosaminidase. TSP-2, which lacks a potentially modifiable serine/threonine in the loop, did not react with CTD110.6. Analysis of tandem modules of TSP-1 localized reactivity of CTD110.6 to EGF-like module 1. Top-down mass spectrometric analysis of EGF-like module 1 demonstrated the expected modifications with glucose (+162 Da) and xylose (+132 Da) separately from modification with N-acetyl hexosamine (+203 Da). Mass spectrometric sequence analysis localized the +203-Da modification to Ser580 in the sequence 575CPPGYSGNGIQC586. These results demonstrate that O-β-N-acetylglucosaminylation can occur on secreted extracellular matrix proteins as well as on cell surface proteins

What makes MUC1 a tumor antigen?

The epithelial mucin 1 (MUC1) is an accepted serum tumor marker and cellular tumor antigen. We discuss recent views on the difference(s) between normal and tumor MUC1, and its implication for the development of cancer vaccines and antibody therapies, with special emphasis on the role of glycosylation

A novel series of anti-human glycophorin A (CD235a) antibodies defining five extra- and intracellular epitopes

Glycophorin A (GPA, CD235a) is a major membrane glycoprotein and marker of cells of the erythroid lineage. It is also the target of Plasmodium falciparum and of influenza virus. We describe a novel series of 10 antibodies towards GPA, recognizing four extra- and intracellular peptide epitopes of this molecule (defined by epitope mapping) and one mixed peptide/carbohydrate epitope. All antibodies bind better to the desialylated than to the fully sialylated molecule, including those specific for the intracellular epitope. For some of the antibodies (representing all five epitopes) functional binding constants were determined by Surface Plasmon Resonance. The new panel complements the already known anti-glycophorin antibodies and offers several potential applications for, e.g., differential diagnosis of erythroleukemias, lineage analyses of erythroid cells, isolation of senescent erythrocytes, or a highly sensitive neuraminidase assay