426 research outputs found

    Water Quality Improvement and Agroforestry Practices

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    This item was presentation # 17 at the April 2007 Water Quality Short Course. More information on the 2007 Water Quality Short Course, including an agenda and links to to other presentations, may be found at http://www.mowin.org/WQSC/April2007/index.htmlFor the items in MOspace regarding the 2007 Water Quality Short Course, please see https://mospace.umsystem.edu/xmlui/browse?value=Water+Quality+Short+Course+2007&type=subjectThis presentation includes two parts. The first part (83 slides) discusses riparian forest buffers, including function, types and design considerations. The second part (68 slides) discusses findings that agroforestry and grass buffers increase water stable soil aggregates and soil enzyme activity

    The Ursinus Weekly, May 29, 1969

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    S.F.A.R.C. blocked on rules proposals • William F. Buckley featured at June 9th Commencement for 304 graduating seniors • 316 freshmen admitted for Fall \u2769 semester • New Vice-Presidents: Pettit, Richter named • IF wrap-up • New members of Sigma Xi Club; 11 students, 4 faculty selected • Editorials: Proper emphasis; Goodbye, and all that • Focus: T.W. Rhody • It\u27s all over • Polemic finale • One-acts reviewed • A story • Eulogy to Dr. Courtney Smith and the one dead in the ghetto • Velikovsky: The outcast • Board meeting • Outing Club ends diversified year • Tacconelli, Maurer finish with highest batting marks • UC netmen hammer way to best log since \u2756 • Final examination schedulehttps://digitalcommons.ursinus.edu/weekly/1176/thumbnail.jp

    The Ursinus Weekly, December 17, 1969

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    Special centennial issue: Ursinus College\u27s 100th Anniversary, 1869-1969 • Editorial: The first hundred years • Focus: Nora Shuler Helfferich, Ursinus College\u27s oldest living alumnus • Reprinted articles: Pa. Governor Martin salutes President McClure and college at ceremony commemorating seventy-fifth anniversary; Ursinus adopts a war time program; Ursinus recipient of new gateway; Apology for peace; Freshman coed evaluates customs, finds friendly spirit on U.C. campus; Customs are savored; Chaperon, Why? Who?; WSGA is frowning on dungarees, slacks for Ursinus campus wear; When Shriner shrieks Ursinus sheiks come dashing from each dorm; Freshman President snatched by Sophs; Ursinus offers special class • Freeland Hall: Don\u27t let it be forgot • Ursinus raises 2,350,000;2,350,000; 550,000 still needed • Search into history substantiates claim of second oldest graduate that Freeland was everything • Ursinus administrators analyze past, present, and future • 1969 season climaxes 76 years • Mangan wins little All-American mention • Bears\u27 football since 1893 • Ray Gurzynski pilots Bear Harriers to 45-4 mark, two championships • Fifty years of girls\u27 field hockey at UC: Bearites to Snell-belles and champs • Albert leads UC Harriers to MAC championship • Footmen end season with 6-win record • 1969 MAC grid statistics • Concise history of Ursinus College: 1869 - present • Ursinus College: One century old, before and afterhttps://digitalcommons.ursinus.edu/weekly/1153/thumbnail.jp

    The Ursinus Weekly, October 10, 1969

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    Seven join, six leave Ursinus faculty • UC and 500 colleges schedule Vietnam moratorium Wednesday; Classes not officially cancelled • William D. Reimert dies, Ursinus Board President • Dr. E. Lewis\u27 math textbook aids Ursinus blind students • Pre-Med convention • Dean Harris weds Bryn Mawr man • Editorial: A very good year • Focus: Mike Stoner in exile • Compulsory convocation: Its validity and purpose • New view of Ursinus • Dr. Rice endorses Vietnam moratorium • Kitchen cynic: Suppose U.C. had mandatory convocations • Perspectives • To eat or not to eat • Board names new members • Spotlight: Mr. Jones, cook • Centennial plans • Opinion: Suggestions for revision of the school calendar • Weaver raps • Faculty portrait: Mrs. Lucas • Woodstock vs. Ursinus • Essay on the new age • Building plans • Freshman class • Harriers extend streak to 26 • Shuman, Mangan potential greats • Flying Dutchmen edge Bears on late T.D. • Ursinus drops grid opener to Diplomats • Registration system analyzed • Lack of needed funds signals impending collapse of agency • Astronaut Scott Carpenter opens Fall Forum series • Ursinus accounting students rank first twice, and secondhttps://digitalcommons.ursinus.edu/weekly/1149/thumbnail.jp

    Stage-Specific Inhibition of MHC Class I Presentation by the Epstein-Barr Virus BNLF2a Protein during Virus Lytic Cycle

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    gamma-herpesvirus Epstein-Barr virus (EBV) persists for life in infected individuals despite the presence of a strong immune response. During the lytic cycle of EBV many viral proteins are expressed, potentially allowing virally infected cells to be recognized and eliminated by CD8+ T cells. We have recently identified an immune evasion protein encoded by EBV, BNLF2a, which is expressed in early phase lytic replication and inhibits peptide- and ATP-binding functions of the transporter associated with antigen processing. Ectopic expression of BNLF2a causes decreased surface MHC class I expression and inhibits the presentation of indicator antigens to CD8+ T cells. Here we sought to examine the influence of BNLF2a when expressed naturally during EBV lytic replication. We generated a BNLF2a-deleted recombinant EBV (ΔBNLF2a) and compared the ability of ΔBNLF2a and wild-type EBV-transformed B cell lines to be recognized by CD8+ T cell clones specific for EBV-encoded immediate early, early and late lytic antigens. Epitopes derived from immediate early and early expressed proteins were better recognized when presented by ΔBNLF2a transformed cells compared to wild-type virus transformants. However, recognition of late antigens by CD8+ T cells remained equally poor when presented by both wild-type and ΔBNLF2a cell targets. Analysis of BNLF2a and target protein expression kinetics showed that although BNLF2a is expressed during early phase replication, it is expressed at a time when there is an upregulation of immediate early proteins and initiation of early protein synthesis. Interestingly, BNLF2a protein expression was found to be lost by late lytic cycle yet ΔBNLF2a-transformed cells in late stage replication downregulated surface MHC class I to a similar extent as wild-type EBV-transformed cells. These data show that BNLF2a-mediated expression is stage-specific, affecting presentation of immediate early and early proteins, and that other evasion mechanisms operate later in the lytic cycle

    Can Monkeys Choose Optimally When Faced with Noisy Stimuli and Unequal Rewards?

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    We review the leaky competing accumulator model for two-alternative forced-choice decisions with cued responses, and propose extensions to account for the influence of unequal rewards. Assuming that stimulus information is integrated until the cue to respond arrives and that firing rates of stimulus-selective neurons remain well within physiological bounds, the model reduces to an Ornstein-Uhlenbeck (OU) process that yields explicit expressions for the psychometric function that describes accuracy. From these we compute strategies that optimize the rewards expected over blocks of trials administered with mixed difficulty and reward contingencies. The psychometric function is characterized by two parameters: its midpoint slope, which quantifies a subject's ability to extract signal from noise, and its shift, which measures the bias applied to account for unequal rewards. We fit these to data from two monkeys performing the moving dots task with mixed coherences and reward schedules. We find that their behaviors averaged over multiple sessions are close to optimal, with shifts erring in the direction of smaller penalties. We propose two methods for biasing the OU process to produce such shifts

    Multi-Wavelength and Multi-Messenger Studies Using the Next-Generation Event Horizon Telescope

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    The next-generation Event Horizon Telescope (ngEHT) will provide us with the best opportunity to investigate supermassive black holes (SMBHs) at the highest possible resolution and sensitivity. With respect to the existing Event Horizon Telescope (EHT) array, the ngEHT will provide increased sensitivity and uv-coverage (with the addition of new stations), wider frequency coverage (from 86 GHz to 345 GHz and higher), finer resolution (<15 micro-arcseconds), and better monitoring capabilities. The ngEHT will offer a unique opportunity to deeply investigate the physics around SMBHs, such as the disk-jet connection, the mechanisms responsible for high-energy photon and neutrino events, and the role of magnetic fields in shaping relativistic jets, as well as the nature of binary SMBH systems. In this white paper we describe some ngEHT science cases in the context of multi-wavelength studies and synergies.https://www.mdpi.com/2075-4434/11/1/17Published versionPublished versio

    Bright Field Microscopy as an Alternative to Whole Cell Fluorescence in Automated Analysis of Macrophage Images

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    Fluorescence microscopy is the standard tool for detection and analysis of cellular phenomena. This technique, however, has a number of drawbacks such as the limited number of available fluorescent channels in microscopes, overlapping excitation and emission spectra of the stains, and phototoxicity.We here present and validate a method to automatically detect cell population outlines directly from bright field images. By imaging samples with several focus levels forming a bright field -stack, and by measuring the intensity variations of this stack over the -dimension, we construct a new two dimensional projection image of increased contrast. With additional information for locations of each cell, such as stained nuclei, this bright field projection image can be used instead of whole cell fluorescence to locate borders of individual cells, separating touching cells, and enabling single cell analysis. Using the popular CellProfiler freeware cell image analysis software mainly targeted for fluorescence microscopy, we validate our method by automatically segmenting low contrast and rather complex shaped murine macrophage cells.The proposed approach frees up a fluorescence channel, which can be used for subcellular studies. It also facilitates cell shape measurement in experiments where whole cell fluorescent staining is either not available, or is dependent on a particular experimental condition. We show that whole cell area detection results using our projected bright field images match closely to the standard approach where cell areas are localized using fluorescence, and conclude that the high contrast bright field projection image can directly replace one fluorescent channel in whole cell quantification. Matlab code for calculating the projections can be downloaded from the supplementary site: http://sites.google.com/site/brightfieldorstaining
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