Feldbestimmungsschlüssel für die Hummeln Österreichs, Deutschlands und der Schweiz (Hymenoptera, Apidae)

GOKCEZADE, J.F., GEREBEN-KRENN, B.-A., NEUMAYER, J., KRENN, H.W. (2010): Feldbestimmungsschlüssel für die Hummeln Österreichs, Deutschlands und der Schweiz (Hymenoptera, Apidae). Linzer biologische Beiträge 42 (1): 5-42, DOI: http://doi.org/10.5281/zenodo.452406

Abb. 11-24 in Feldbestimmungsschlüssel für die Hummeln Österreichs, Deutschlands und der Schweiz (Hymenoptera, Apidae)

Abb. 11-24: Schematische Zeichnungen der Körperteile von Arbeiterinnen und Königinnen; (11)- (13) linke Antenne, Pfeil weist auf das 3. Antennenglied, (11) nach B. monticola, (12) von B. mendax, (13) von B. confusus; (14)+(15) Metasoma in Seitenansicht ohne Behaarung gezeichnet, (14) nach ventral gekrümmt, (15) nicht nach ventral gekrümmt; (16) Kopf sehr lang; (17) Kopf kurz; (18) rechte Mandibel von B. wurflenii; (19) rechte Mandibel mit zwei Zähnen und geradem Kaurand (nach B. lapidarius); (20) Rückenansicht T6 von B. rupestris; (21)+(22) rechter Scapus von (21) B. sylvestris, (22) B. norvegicus und B. flavidus; (23)+(24) Rückenansicht T6 von (23) B. lapidarius, (24) B. sichelii.Published as part of GOKCEZADE, J.F., GEREBEN-KRENN, B.-A., NEUMAYER, J. & KRENN, H.W., 2010, Feldbestimmungsschlüssel für die Hummeln Österreichs, Deutschlands und der Schweiz (Hymenoptera, Apidae), pp. 5-42 in Linzer biologische Beiträge 42 (1) on page 17, DOI: 10.5281/zenodo.452406

A Combination of CRISPR/Cas9 and Standardized RNAi as a Versatile Platform for the Characterization of Gene Function

Traditional loss-of-function studies in Drosophila suffer from a number of shortcomings, including off-target effects in the case of RNA interference (RNAi) or the stochastic nature of mosaic clonal analysis. Here, we describe minimal in vivo GFP interference (miGFPi) as a versatile strategy to characterize gene function and to conduct highly stringent, cell type-specific loss-of-function experiments in Drosophila. miGFPi combines CRISPR/Cas9-mediated tagging of genes at their endogenous locus with an immunotag and an exogenous 21 nucleotide RNAi effector sequence with the use of a single reagent, highly validated RNAi line targeting this sequence. We demonstrate the utility and time effectiveness of this method by characterizing the function of the Polymerase I (Pol I)-associated transcription factor Tif-1a, and the previously uncharacterized gene MESR4, in the Drosophila female germline stem cell lineage. In addition, we show that miGFPi serves as a powerful technique to functionally characterize individual isoforms of a gene. We exemplify this aspect of miGFPi by studying isoform-specific loss-of-function phenotypes of the longitudinals lacking (lola) gene in neural stem cells. Altogether, the miGFPi strategy constitutes a generalized loss-of-function approach that is amenable to the study of the function of all genes in the genome in a stringent and highly time effective manner

The NiCE Discussion Room: Integrating Paper and Digital Media to Support Co-Located Group Meetings

Current technological solutions that enable content creation and sharing during group discussion meetings are often cumbersome to use, and are commonly abandoned for traditional paper-based tools, which provide flexibility in supporting a wide range of working styles and task activities that may occur in a given meeting. Paper-based tools, however, have their own drawbacks; paper-based content is difficult to modify or replicate. We introduce a novel digital meeting room design, the NiCE Discussion Room, which integrates digital and paper tools into a cohesive system with an intuitive pen-based interface. The combination of digital and paper media provides groups with a flexible design solution that enables them to create, access, and share information and media from a variety of sources to facilitate group discussions. This paper describes the design solution, along with results from a user study conducted to evaluate the usability and utility of the system.Ye