Genome Sequence of Kurthia Type Species Kurthia zopfii Strain ATCC 33403T

The genome of the type strain of the Kurthia genus, Kurthia zopfii ATCC 33403, was sequenced. Nonpathogenic K. zopfii has been isolated from intestinal contents, fecal material, meats, meat products, milk, water, and air, including air at high altitudes. The predicted genome size is 2,878,279 bp, with 37.05% G+C content

Genome Sequences for Three Strains of Kocuria rosea, Including the Type Strain

Genomes from three strains of Kocuria rosea were sequenced. K. rosea ATCC 186, the type strain, was 3,958,612 bp in length with a total G+C content of 72.70%. When assembled, K. rosea ATCC 516 was 3,862,128 bp with a 72.82% G+C content. K. rosea ATCC 49321 was 4,018,783 bp in size with a 72.49% G+C content

Writing On the Wall: Social Media the First 2,000 Years

Book Review over Tom Standage's book Writing On the Wall: Social Media the First 2,000 Year

Impact of Cell Density on Cx43 Expression in Endothelial Cells

Consumption of omega-3 fatty acids benefits the cardiovascular system by reducing blood vessel inflammatory responses. Our recent work showed omega-3 fatty acids decrease connexin 43 (Cx43) expression in cultured endothelial cells after 1 day. Our interest in Cx43 was as marker of inflammation as omega-3 fatty acids are strongly implicated as anti-inflammatory agents. Localized inflammatory responses drive the growth of the atherosclerotic plaques responsible for a majority of cardiovascular diseases. Anything that affects the inflammatory response will affect the growth (and stability which is also important) of those plaques. This poster will summarize current knowledge of atherosclerosis and inflammation as well as the impact of omega-3 fatty acids on inflammation

Draft Genome Sequence of Xanthobacter tagetidis ATCC 700314T

Xanthobacter tagetidis is a thiophene-degrading bacterium associated with root balls of the plant genus Tagetes, which includes marigolds. It is a Gram-negative facultatively autotrophic bacterium with pleomorphic morphology exhibiting bent and branching rods. From strain TagT2CT (= ATCC 700314T), we report a genome assembly of 4,945,221 bp and a 69.5% G+C content

Effect of stem age on the response of stem diameter variations to plant water status in tomato

Plant water status plays a major role in glasshouse cultivation of tomato (Solanum lycopersicum L.). New climate control technologies alter the glasshouse climate and make it less dependent on solar radiation. However, irrigation strategies are still often based on solar radiation sums. In order to maintain a good plant water status, it is interesting to use plant-based methods such as monitoring sap flow (F) or stem diameter variations (SDV). Though SDV give important information about plant water status, an unambiguous interpretation might be difficult because other factors such as stem age, fruit load and sugar content of the stem also affect SDV. In this study, an analysis of the effect of stem age on the response of SDV to water status was performed by calibration of a mechanistic flow and storage model. This allowed us to determine how parameter values changed across the growing season. Tissue extensibility decreased over the growing season resulting in a lower growth rate potential, whereas daily cycles of shrinking and swelling of the stem became more pronounced towards the end of the growing season. Parameters were then adapted to time-dependent variables and implemented in the model, allowing long term simulation and interpretation of SDV. Sensitivity analysis showed that model predictions were very sensitive to initial sucrose content of the phloem tissue and the parameters related to plastic growth

Reliable quantification of 1,2-dihydroxynaphthalene in urine using a conjugated reference compound for calibration.

After environmental and occupational exposure to naphthalene, 1,2-dihydroxynaphthalene (1,2-DHN) was shown to be one major metabolite in human naphthalene metabolism. However, the instability of free 1,2-DHN complicates the reliable determination of this promising biomarker in urine. To solve this stability problem, glucuronide conjugates of 1,2-DHN and the corresponding isotopically labelled D-6-1,2-dihydroxynaphthalene (D-6-1,2-DHN) were synthesised and applied as reference material and internal standard in a gas chromatographic-tandem mass spectrometric (GC-MS/MS) method. The determination of 1- and 2-naphthol (1-MHN, 2-MHN) was included in the procedure to enable a comprehensive assessment of naphthalene metabolism and exposure. The results of the validation showed a high reliability and sensitivity of the method. The detection limits range from 0.05 to 0.16 mu g/L. Precision and repeatability were determined to range from 1.4 to 6.6% for all parameters. The simultaneous determination of 1- and 2-MHN as additional parameters besides 1,2-DHN enables the application of the method for further metabolism and kinetic studies on naphthalene. The use of glucuronide-derivative reference substances and the application of structurally matched isotopic-labelled internal standards for each substance guarantee a reliable quantification of the main naphthalene metabolites 1,2-DHN and 1- and 2-MHN

Regulatory Fit Explains Students’ Emotional Responses to Graded Speech Assignments

Students’ emotional responses often provide valuable indicators of whether they are languishing or flourishing in their first-year classes, including introductory communication courses. Grading often exerts a strong influence on students’ emotions. However, though students generally have positive moods after receiving high marks and negative ones when their grades are low, the intensity of these responses varies considerably. The current study examines whether Higgins’ (2012) regulatory fit theory accounts for students’ differing moods after receiving grades on introductory speech assignments. According to this perspective, prevention focus students use vigilance to avoid adverse outcomes. Thus, low evaluations provide a regulatory fit for prevention focus students by evoking feelings of alarm or distress that increase vigilance. Promotion focus students use eagerness to make gains progressively. High grades create a regulatory fit for promotion focus students by producing feelings of excitement that contribute to eagerness. When grading does not provide regulatory fit, prevention, and promotion focus, students will experience feelings of relief and discouragement. In the current study, each of these hypotheses was confirmed and support the principle that the fit between student regulatory focus and feedback sign explain student emotional responses to grading. These findings contribute to research and pedagogy in the introductory communication course. Keywords: Instructional feedback, grading, feedback sign, regulatory fit, prevention focus, promotion focus, regulatory congruence, public speaking