Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

Epidemiologic study of ankle fractures in a tertiary hospital

OBJECTIVES: To evaluate the epidemiology of ankle fractures surgically treated at the Instituto de Ortopedia e Traumatologia do Hospital das Clínicas da Universidade de São Paulo.METHODS: Medical records of patients admitted with foot and ankle fractures between 2006 and 2011 were revised. Seventy three ankle fractures that underwent surgical treatment were identified. The parameters analyzed included age, gender, injured side, AO and Gustilo & Anderson classification, associated injuries, exposure, need to urgent treatment, time to definitive treatment and early post-operative complications. Study design: retrospective epidemiological study.RESULTS: Male gender was predominant among subjects and the mean age was 27.5 years old. Thirty nine fractures resulted from traffic accidents and type B fracture according to AO classification was the most common. Twenty one were open fractures and 22 patients had associated injuries. The average time to definitive treatment was 6.5 days. Early post-operative complications were found in 21.3% of patients.CONCLUSIONS: Ankle fractures treated in a tertiary hospital of a large city in Brazil affect young people victims of high-energy accidents and present significant rates of associated injuries and post-operative complications. Level of Evidence IV, Cases Series

Gravimetric composition of municipal solid waste disposed in a large-scale experimental cell in Southeastern Brazil

The increasing consumption in emerging countries like Brazil has caused the increased generation of municipal solid waste (MSW) and hence the demand for environmentally friendly and relatively inexpensive disposal of the waste, such as sanitary landfills. In this research, it is presented the gravimetric composition of MSW disposed in a large-scale experimental cell, built on a sanitary landfill, Campinas, state of São Paulo, Brazil. For such, it was adopted the hypothesis that the characteristics of MSW vary with the income level of the population and the type of activity that generates it (domestic or commercial). Twenty-two tests were performed for gravimetric characterisation. Statistical analyses considering the results of this characterisation showed that the adopted hypothesis was correct. The MSW composition was preponderant by organic matter (52.7%), paper (17.5%) and plastic (13.0%). The methodology used was considered feasible to achieve the proposed objective of the characterisation17212814

Avaliações físicas, químicas e biológicas da microbacia do Córrego Modeneis em Limeira â SP

Este trabalho apresenta uma avaliação da qualidade da água do córrego Modeneis pertencente à microbacia do córrego Barroca Funda, afluente do Ribeirão Tatu, na cidade de Limeira, SP. Foram escolhidos três pontos de coleta para análises: nascente, meio e final da microbacia. O estudo foi realizado através do monitoramento de águas e sedimento durante oito semanas, sendo verificados os parâmetros físicos (pH, cor, turbidez, condutividade, sólidos totais dissolvidos), químicos (alcalinidade, acidez, nitrato, dureza e TOC) e biológicos (toxicidade aguda â Daphnia similis e crônica â Ceriodaphnia dubia) das amostras. Palavras-chave: corpo dâágua, sedimento, ecotoxicidade, Daphnia similis, Ceriodaphnia dubia. ABSTRACT This work presents an evaluation of the water quality from Modeneis stream belonging the microbasin of Barroca Funda stream, affluent of the Ribeirão Tatu in the city of Limeira, São Paulo. Three points of collection for analyses had been chosen: spring, middle and end of microbasin. The study was realized through the monitoring of waters and sediment during eight weeks, being verified the physical parameter (pH, Color, Turbidity, Conductivity and Total Solids), chemical (Alkalinity, Acidity, Nitrate, Hardness and TOC) and biological ( acute Toxicity- Daphnia similis and chronic- Ceriodaphnia dubia) of the samples. Keywords: water body, sediment, ecotoxicity, Daphnia similis, Ceriodaphnia dubia.</div

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly