Konceptualni model za istraživanje faktora koji utječu na dopiranje do glasa korisnika

Since the voice of the customer (VoC) could be of core importance for an organisation\u27s success, it is of high significance to identify the motivating and demotivating factors that influence the customers\u27 intention to provide or not to provide feedback on service quality. This study aims to observe how awareness on the issue of providing feedback, personal beliefs on the impact of feedback, expectations from the company, and organisational culture impact the customers\u27 decision to leave feedback. To explore the influence of the chosen factors an online survey was conducted and the Structural Equation Modeling (SEM) analysis was employed. The results show that awareness and organisational culture have a positive impact on the customers\u27 decision to provide feedback, while the expectations from the company have a negative impact on such customer behaviour. The presented conceptual model might provide novel viewpoints into the factors which impact customers\u27 behaviour regarding their decisions to provide feedback and initiate further studies on the topic of VoC.Budući da bi glas korisnika (VoC) mogao biti od presudne važnosti za organizacijski uspjeh, dragocjeno je za organizaciju da pravodobno identificira motivirajuće i demotivirajuće faktore koji utječu na volju korisnika da ostave ili ne ostave povratne informacije o kvaliteti usluge. Cilj je ovog istraživanja promatrati kako svijest o pružanju povratnih informacija, osobna uvjerenja o utjecaju povratnih informacija, očekivanja od kompanije i organizacijska kultura utječu na odluku korisnika da ostave povratne informacije o kvaliteti proizvoda ili usluge. Da bi se ispitao utjecaj odabranih četiriju faktora, provedena je online anketa i analiza modeliranja strukturne jednadžbe (SEM). Rezultati pokazuju da svijest i organizacijska kultura pozitivno utječu na odluku korisnika da ostavi povratnu informaciju, dok očekivanja od kompanije negativno utječu na takvo ponašanje korisnika. Predstavljeni konceptualni model mogao bi osigurati nova gledanja na faktore koji utječu na ponašanje korisnika u vezi s njihovim odlukama da ostave povratne informacije i pokrenuti nova istraživanja na temu glasa korisnika (VoC)

Reakcija genotipova pšenice na suvišak bora u kulturi in vitro

The objective of this study was to evaluate boron tolerance of wheat genotypes using mature embryo culture. The analysis involved 79 recombinant inbred lines of the International Triticeae Mapping Initiative (ITMI) population and three Serbian varieties with known boron (B) tolerance (Pobeda – sensitive S, Balerina -medium tolerant MT, and Nevesinjka – tolerant T). The evaluation was performed on a modified MS medium to which 15 mM of boric acid was added. The control medium contained no excess B. Callus fresh weight (CFW) and reduction of fresh callus weight (RFCW) were determined after one month of cultivation. ANOVA has shown highly significant effect of genotype, the media, and their interaction to callus tissue growth and also significant genotypic effect on RFCW. Majority of genotypes (39) had sensitive reaction to excess boron, twenty-three were medium tolerant, while four of them were tolerant. The obtained results can be potentially used for mapping QTLs associated with tolerance to excess B in wheat breeding program.Cilj ovog istraživanja bio je da se proceni tolerantnost genotipova pšenice na suvišak bora primenom kulture zrelog embriona. Analizom je obuhvaćeno 79 rekombinantnih inbred linija ITMI mapirajuće populacije i 3 sorte poznate tolerantnosti na bor (Pobeda – osetljiva, Balerina – srednje tolerantna i Nevesinjka – tolerantna) poreklom iz Srbije. Procena tolerantnosti je rađena na modifikovanoj MS hranljivoj podlozi u koju je dodato 15 mM borne kiseline. Kontrolna podloga nije sadržala suvišak bora. Sveža masa kalusa i redukcija sveže mase kalusa, određene su nakon mesec dana gajenja. ANOVA je pokazala veoma značajan efekat genotipa, hranljive podloge i njihove interakcije na rast kalusa, kao i značajan genotipski efekat na redukciju sveže mase kalusa. Većina genotipova (39) je imala osetljivu reakciju na suvišak bora, 23 su bila srednje tolerantna dok su 4 genotipa bila tolerantna. Dobijeni rezultati se potencijalno mogu koristiti za mapiranje gena i QTL-ova povezanih sa tolerancijom na suvišak B u oplemenjivanju pšenice

Xenobiotic CAR activators induce Dlk1-Dio3 locus non-coding RNA expression in mouse liver

Predicting the impact of human exposure to chemicals such as pharmaceuticals and agrochemicals requires the development of reliable and predictive biomarkers suitable for the detection of early events potentially leading to adverse outcomes. In particular, drug-induced non-genotoxic carcinogenesis (NGC) during preclinical development of novel therapeutics intended for chronic administration in humans is a major challenge for drug safety. We previously demonstrated Constitutive Androstane Receptor (CAR) and WNT signaling-dependent up-regulation of the pluripotency associated Dlk1-Dio3 imprinted gene cluster non-coding RNAs (ncRNAs) in the liver of mice treated with tumorpromoting doses of phenobarbital (PB). Here, to explore the sensitivity and the specificity of this candidate liver tumor promotion ncRNAs signature we compared phenotypic, transcriptional and proteomic data from wild-type, CAR/PXR double knock-out and CAR/PXR double humanized animals treated with tumor-promoting doses of PB or chlordane, both well-established CAR activators. We further investigated selected transcriptional profiles from mouse liver samples exposed to seven NGC compounds working through different mode of actions, overall suggesting CAR-activation specificity of the Dlk1-Dio3 long ncRNAs activation. We propose that Dlk1-Dio3 long ncRNAs up-regulation is an early CAR-activation dependent transcriptional signature during xenobiotic-induced mouse liver tumor promotion. This signature may further contribute mode of action-based ‘weight of evidence’ cancer risk assessment for xenobiotic-induced rodent liver tumors

Making reliable negative predictions of human skin sensitisation using an in silico fragmentation approach

A previously published fragmentation method for making confident negative in silico predictions has been applied to the problem of predicting skin sensitisation in humans, making use of a dataset of over 2,750 chemicals with publicly available skin sensitisation data from 18 in vivo assays. An assay hierarchy was designed to enable the classification of chemicals within this dataset as either sensitisers and non-sensitisers where data from more than one in vivo test was available. The negative prediction approach was validated internally, using a 5-fold cross-validation, and externally, against a proprietary dataset of approximately 1,000 chemicals with in vivo reference data shared by members of the pharmaceutical, nutritional, and personal care industries. The negative predictivity for this proprietary dataset was high in all cases (>75%), and the model was also able to identify structural features that resulted in a lower accuracy or a higher uncertainty in the negative prediction, termed misclassified and unclassified features respectively. These features could serve as an aid for further expert assessment of the negative in silico prediction

Determination of Permitted Daily Exposure for Topical Ocular Drugs

Limits for the carryover of product residues should be based on toxicological evaluation such as described in the European Medicines Agency (EMA) Guideline on setting health based exposure limits for use in risk identification in the manufacture of different medicinal products in shared facilities (EMA, 2014).Currently no guidance on setting PDE is available for ocular drug substances. The purpose of this study was to identify a method for calculating a PDE value for topically administered ocular drugs (PDEocular). The method can be applied in cross-contamination risk assessment during manufacturing of drugs intended for topical ocular administration, as well. We have examined the pharmacokinetic and pharmacodynamic (PKPD) properties of the drugs administered topically to the eyes and compared them to the PKPD parameters of systemically administered drugs. Furthermore we examined the therapeutic doses and critical effects of drugs administered topically to the eyes. In this manuscript we are proposing a method for calculation of PDEocular for manufacturing of drugs in a facility dedicated for ocular drugs. Additionally we are proposing a conversion factor for systemically administered drugs that are manufactured before the ocular drug