Sand transport in multiphase flow mixtures in a horizontal pipeline: An experimental investigation

An inherent problem with both oil and natural gas production is the deposition of sand particles in pipeline, which could lead to problems such as excessive pressure drops, equipment failure, pipeline erosion, and production decline. The characterization of sand particles transport and sedimentation in different flow systems such as sand–multiphase mixtures is vital to predict the sand transport velocity and entrainment processes in oil and gas transportation pipelines. However, it seems that no model exists able to accurately characterize the sand transport and deposition in multiphase pipeline. In fact, in the last decade several researchers tried to extend the modeling of liquid-solid flow to gas-liquid-solid flow, but no significant results have been obtained, especially in slug flow condition due to the complexity of the phenomenon. In order to develop and validate a mathematical model properly formulated for the calculation of the sand critical deposition velocity in gas-liquid flow, more and more experimental data are necessary. This paper presents a preliminary experimental study of three phase flows (air-water-sand) inside a horizontal pipe and the application of the sand-liquid models present in literature. Significant observations were made during the experimental study from which several conclusions were drawn. Different sand flow regimes were established by physical observation and data analysis: fully dispersed solid flow, moving dunes and stationary bed. The critical deposition velocities were determined at different sand concentrations. It was concluded that sand transport characteristics and the critical deposition velocity are strongly dependent on the gas-liquid flow regime and on sand concentration. Keywords: Sand transport, Multiphase flow, Minimum transport condition, Deposition, Experimental investigation, Oil and ga

Variable number of tandem repeat polymorphisms of the interleukin-1 receptor antagonist gene IL-1RN: a novel association with the athlete status

<p>Abstract</p> <p>Background</p> <p>The interleukin-1 (IL-1) family of cytokines is involved in the inflammatory and repair reactions of skeletal muscle during and after exercise. Specifically, plasma levels of the IL-1 receptor antagonist (IL-1ra) increase dramatically after intense exercise, and accumulating evidence points to an effect of genetic polymorphisms on athletic phenotypes. Therefore, the IL-1 family cytokine genes are plausible candidate genes for athleticism. We explored whether IL-1 polymorphisms are associated with athlete status in European subjects.</p> <p>Methods</p> <p>Genomic DNA was obtained from 205 (53 professional and 152 competitive non-professional) Italian athletes and 458 non-athlete controls. Two diallelic polymorphisms in the IL-1β gene (<it>IL-1B</it>) at -511 and +3954 positions, and a variable number tandem repeats (VNTR) in intron 2 of the IL-1ra gene (<it>IL-1RN</it>) were assessed.</p> <p>Results</p> <p>We found a 2-fold higher frequency of the <it>IL-1RN </it>1/2 genotype in athletes compared to non-athlete controls (OR = 1.93, 95% CI = 1.37-2.74, 41.0% vs. 26.4%), and a lower frequency of the 1/1 genotype (OR = 0.55, 95% CI = 0.40-0.77, 43.9% vs. 58.5%). Frequency of the <it>IL-1RN </it>2/2 genotype did not differ between groups. No significant differences between athletes and controls were found for either -511 or +3954 <it>IL-1B </it>polymorphisms. However, the haplotype (-511)C-(+3954)T-(VNTR)2 was 3-fold more frequent in athletes than in non-athletes (OR = 3.02, 95% CI = 1.16-7.87). Interestingly, the <it>IL-1RN </it>1/2 genotype was more frequent in professional than in non-professional athletes (OR = 1.92, 95% CI = 1.02-3.61, 52.8% vs. 36.8%).</p> <p>Conclusions</p> <p>Our study found that variants at the IL-1ra gene associate with athletic status. This confirms the crucial role that cytokine IL-1ra plays in human physical exercise. The VNTR <it>IL-1RN </it>polymorphism may have implications for muscle health, performance, and/or recovery capacities. Further studies are needed to assess these specific issues. As VNTR <it>IL-1RN </it>polymorphism is implicated in several disease conditions, athlete status may constitute a confounding variable that will need to be accounted for when examining associations of this polymorphism with disease risk.</p