Relationship Between Attrition Faces and Signs of TMD

Smatra se da su brusne fasete znak funkcijskih i parafunkcijskih aktivnosti, te ih nalazimo na okluzalnim i incizalnim plohama zuba obično kao posljedicu procesa atricije. Brusne fasete razlikuju se opsegom i pozicijom na zubima. Bruksizam, kao parafunkcijska aktivnost, smatra se jednim od etioloških čimbenika za nastanak disfunkcije stomatognatoga sustava. Cilj rada bio je istražiti moguću povezanost između pojavnosti brusnih faseta, kao znak bruksizma, i znakova temporomandibularne disfunkcije. Temeljem nalaza brusnih faseta nakon izvršena kliničkog pregleda izdvojeno je 100 ispitanika, određenih za daljnje istraživanje. Opsežnost brusnih faseta procjenjivana je modificiranim indeksom Pullingera i Seligmana: stupanj 0 = nema vidljive atricije; 1 = minimalna atricija kvržica ili incizalnih bridova (u caklini); 2 = fasete usporedne s normalnim područjima kontura ploha (caklina); 3 = zamjetna zaravnjenja kvržica ili incizalnih bridova (caklina); 4 = potpuni gubitak kontura ploha/bridova i ekspozicija dentina do polovice visine nekadašnje krune zuba; 5 = potpuni gubitak kontura i ekspozicija dentina za više od polovice nekadašnje krune zuba. Svi ispitanici podvrgnuti su kliničkim ispitivanjima kako bi se utvrdilo postojanja znakova TMD-a. Rezultati su pokazali da ne postoji statistički znatna povezanost između znakova TMD-a i pojavnosti brusnih faseta te ni između znakova TMD-a i opsežnosti brusnih faseta. Rezultati ovoga istraživanja slažu se s ostalim recentnim studijama da brusne fasete nisu pouzdan pokazatelj za procjenjvanje funkcijskog stanja stomatognatoga sustava. Ovim istraživanjem nije utvrđeno postojanje povezanosti između pojavnosti brusnih faseta i znakova temporomandibularne disfunkcije.Attrition faces are usually seen on the occlusal or incisal surfaces of teeth worn by attrition. They are considered to be a sign of functional and parafunctional activities, and can differ by wideness and position on the teeth. Bruxizm, as a parafunctional activity, is considered to be a risk factor for dysfunction of the masticatory system. The aim of the study was to investigate the possible relationship between attritional faces, as a sign of bruxizm, and signs of temporomandibular dysfunction. By clinical examination, a group of 100 subjects was selected for trial, based on findings of attritional faces. The severity of attrition faces was quantified on a fivepoint scale ( modified assessment for determination of incisal tooth wear according to Pullinger and Seligman: 0 = no visible tooth wear; 1 = minimal wear of cusps or incisal tips (enamel); 2 = faces parallel to normal planes of contour (enamel); 3 = noticeable flattening of cusp or incisal edges (enamel); 4 = total loss of contour and dental exposure when identifiable (dentin exposure up to half of former crown of tooth): 5 = total loss of contour and dental exposure over half of former crown of tooth). Selected subjects were then examined by standard procedure to investigate the presence of TMD signs. Results showed that there was no statistical significance between signs of TMD and presence of attrition faces, and neither between signs of TMD and severity of attrition faces. Results of this study are compatible with other recent investigations, which show that attrition faces are not a reliable sign for assessing the functional status of the masticatory system. According to this investigation there is no association between attrition faces and signs of temporomandibular dysfunction

Occlusal Interferences in Localised and Generalized Periodontitis

Značaj okuzijskih zapreka u razvoju i u terapiji parodontnih bolesti i poremećaja iznimno je kontroverzan. Još uvijek su u najstručnijim krugovima znanstvenika aktualne rasprave o povezanosti zapreka i okluzijske traume i o njihovoj ulozi u parodontnoj bolesti. Svrha rada bila je istražiti postoji li statistički znatna razlika u pojavnosti okluzijskih zapreka ( na radnoj i balansnoj strani) između skupine pacijenata s lokaliziranim parodontitisom i skupine pacijenata s generaliziranim parodontitisom. Ispitanici su bili pacijenti s parodontnom bolesti, koji su klasificirani prema Extent and Severity Indexu (ESI) na ispitivanu skupinu lokaliziranih parodontitisa i ispitivanu skupinu generaliziranih parodontitisa. Kretnje na laterotruzijskoj strani/kontakti u interkaninom području tijekom protruzijske kretnje bilježene su plavim artikulacijskim papirom debljine 12µ, a kontakti na mediotruzijskoj strani/kontakti u lateralnim područjima tijekom protruzijske kretnje bilježeni su crvenim artikulacijskim papirom debljine 12µ. U obje ispitivane skupine 45,12% ispitanika imalo je zapreke na radnoj strani, a 48,78% ispitanika imalo je zapreke na balansnoj strani. U skupini lokaliziranoga parodontitisa 33,33% ispitanika imalo je zapreke na radnoj strani, a 50,81% ispitanika imalo ih je u skupini generaliziranoga parodontitisa. Zapreke na balansnoj strani postojale su u 52,38% ipitanika u skupini lokaliziranoga parodontitisa, a 47,54% ispitanika imalo ih je u skupini generaliziranoga parodontitisa. Hi-kvadrat testom utvrđeno je da ne postoji statistički znatna razlika između pojavnosti okluzijskih zapreka u skupini ispitanika s lokaliziranim parodontitisom u usporedbi sa skupinom ispitanika s generaliziranim parodontitisom. Iako nema statistički znatne razlike, postoji tendencija razlike u frekvencijama (p = 0,054) te je vidljivo da u skupini ispitanika s generaliziranim parodontitisom postoji više slučajeva bez zapreka na balansnoj strani (52,45%) u odnosu prema skupini ispitanika s lokaliziranim parodontitisom (47,61%). Rezultati ovog istraživanja pokazuju da ne postoji statistički znatna razlika u pojavnosti okluzijskih zapreka u skupini ispitanika s lokaliziranim parodontitisom i u skupini ispitanika s generaliziranim parodontitisom.The significance of occlusal interference in the development and therapy of periodontal disease is very controversial. There are still very vivid discussions going on between experts whether or not interferences cause occlusal trauma, and wath is its role in periodontal disease. The aim of the study was to investigate whether there i statistically significant difference in the manifestation of occlusal interferences (working side and balancing side) among a group of patients sufferig from localised periodontitis and a group of patients with generalized periodontitis. Examinees were patients with periodontal disease, classified according to Extent and Severity Index (ESI) into a group with localised periodontitis and a group with generalized peiodontitis. Movements on the laterotrusion side/contacts in the intercanine segment in protrusion were marked with blue articulation paper 12µ thick, while contacts on the mediotrusion side/contacts in lateral segments during protrusion were marked with red articulation paper (12µ thick). Of al patients, there were 45.12% with working side interferences, and 48.78% with balancing side interferences in both examined groups. 33.33% of the examinees in the group with localised periodontitis had working side interferences, and 50.81% of the examinees in the group with generalized periodontitis. Balancing side interferences were found in 52.38% of patients in the group with localised periodontitis, and 47.54% of patients in the group with generalized periodontitis. Chi-square test showed that there was statistically significant difference between manifestation of occlusal interferences in the group of patients with localised periodontitis compared to manifestation of articulation interferences in the group of patients with generalized periodontitis (chi-square = 3.561; p = 0.313). Although there was no statistically significant difference, there was a tendency to difference in frequencies (p = 0.054), and it is shown that in the group of patients with generalized periodontitis there were more cases with no balancing side interferences on any side (52.45%), compared to the group of patients with localised periodontitis (47.61%). Results showed that there was no difference in manfestation of occlusal interferences in localised peiodontitis compared to generalized periodontitis

Build up of yield stress fluids via chaotic emulsification

Stabilised dense emulsions display a rich phenomenology connecting microstructure and rheology. In this work, we study how an emulsion with a finite yield stress can be built via large-scale stirring. By gradually increasing the volume fraction of the dispersed minority phase, under the constant action of a stirring force, we are able to achieve a volume fraction close to (Formula presented.). Despite the fact that our system is highly concentrated and not yet turbulent we observe a droplet size distribution consistent with the (Formula presented.) scaling, often associated with inertial range droplets breakup. We report that the polydispersity of droplet sizes correlates with the dynamics of the emulsion formation process. Additionally, we quantify the visco-elastic properties of the dense emulsion finally obtained and we demonstrate the presence of a finite yield stress. The approach reported can pave the way to a quantitative understanding of the complex interplay between the dynamics of mesoscale constituents and the large-scale flow properties of yield stress fluids

The Relationship Between Type of Occlusion and TMJ Sounds

Utjecaj okluzije na nastanak zvuka u temporomandibularnom zglobu nije potpuno potvrđen. Svrha je ovog istraživanja bila utvrditi utjecaj okluzijskih koncepcija na nastanak zvuka u temporomandibularnome zglobu. Ispitivana skupina sastojale se je od 96 ispitanika u dobi od 24 - 52 godine (x = 35,03 ± 6,92). Okluzijske koncepcije određene su kliničkim pregledom. U ovisnosti o kontaktima na laterotruzijskoj i meziotruzijskoj strani ispitanici su kategorizirani u tri skupine (okluzija vođena očnjakom, grupna funkcija, te bilateralno uravnotežena okluzija). Kliničkim pregledom te auskultacijom s pomoću stetoskopa registrirano je postojanje zvuka. 70,83 % ispitanika imalo je okluziju vođenu očnjakom, 16,66 % grupnu funkciju, a 12,5 % bilateralno uravnoteženu okluziju. Zvuk u temporomandibularnom zglobu postojao je u 41,6 % slučajeva. Rezultati statističke raščlambe (Pearson ?2) pokazuju da između skupina nema statistički znatne razlike (?2 = 2,09 p = 0,351). Rezultati ovog istraživanja upućuju na zaključak da okluzijske koncepcije nemaju utjecaja na nastanak zvuka u temporomandibularnom zglobu.The influence of occlusion on the occurrence of sound in the temporomandibular joint had not been complately proved. The objective of this investigation was to determine the effect of type of occlusion on the occurrence of sounds in the TMJ. A group of 96 subjects, aged from 24-52 years (x = 35.03 ± 6.92) was examined. The type of occlusion was dentermined by clinical examination. Depending on the contacts on the laterotrusal and mediotrusal side the subjects were categorized into three groups (canine guided occlusion, group function and balanced occlusion). The existence of sounds was registred by means of a clinical examination and auscultation by stethoscope. In the examined group 70.83% of examinees had canine guidance, 16.66 % group function and 12.5 % balanced occlusion. Temporomandibular joint sound was present in 41.6 % of subjects. The results of the statistical analysis (Pearson ?2) shows no statistically significant difference between these 3 groups (?2 = 2.09 p = 0.351). The results of this study suggest that the type of occlusion does not have an influence on the occurrence of sound in the TMJ

The Relationship Between Occlusion and Temporomadibular Disorders

Etiologija temporomandibularnih poremećaja i može bitini utjecaj nisu potpuno razjašnjeni. Svrha istraživanja bila je utvrditi utjecaj okluzijskih odnosa na funkciju stomatognatoga sustava. Ispitivana skupina sastojala se je od 96 ispitanika u dobi od 24-52 godine (x = 35,03 ± 6,92). Kliničkim pregledom utvrđen je broj zuba, broj zuba u okluziji, okluzijska koncepcija (okluzija vođena očnjakom, grupna funkcija i bilateralno uravnotežena okluzija). Izjereni su iznosi okomitog preklopa i vodoravnog pregriza. RKP položaj određen je chin-point tehnikom i fiksiran Luciajigom. Klizanje iz RKP u IKP položaj izmjereno je u anteroposteriornom, okomitom i postraničnome smjeru. Mjerenja maksimalnih kretnji izvršena su s pomoću precizne pomične mjerke. Registrirana je možebitna pojava zvuka i boli. Izvršena je palpacija temporomandibularnih zglobova, žvačnih i vratnih mišića. Izračunani su Helkimov okuzijski, anamnestiči i klinički disfunkcijski indeks (indeks mandibularne pokretljivosti, indeks funkcije temporomandibularnoga zgloba, indeks bolnosti mandibularnih kretnji te indeks mišićne i zglobne boli). 3,21% ispitanika bilo je bez okluzijskih poremećaja (Oi0), 52,32% imalo je umjerene okluzijske poremećaje (OiI), a 38,56% imalo je ozbiljne okluzijske poremećaje (OiII). U usporedbi s anamnestičkim i kliničkim disfunkcijskim indeksom Pearson ?2 test pokazuje da ozbiljni okluzijski poremećaji nisu povezani sa ozbiljnim disfunkcijskim poremećajima. Razlika među skupinama nije statistički znatna (p > 0,05). Rezultati ovoga istraživanja upućuju na zaključak da okluzija nema utjecaja na nastanak i tijek TMD-a.The etiology of temporomandibular disorders and the role of occlusion, has still not been entirely clarified. The objective of this investigation was to determine the effect of occlusal relationship on the function of the stomatognathic system. A group of subjects, aged from 24-52 years (x = 35.03 ± 6.92) was examined. The number of teeth, the number of teeth in occlusion, type of occlusion (canine guided occlusion, group function and balanced occlusion) was determined by clinical examination. Overbite and overjet were measured. RCP position was determined by chinpoint technique and fixing by Lucia-jig. The sliding from RCP to IKP position was determined in the anteroposterior, vertical and lateral direction. A precise calliper was used to measure maximal jaw movement. Pain and sounds were registred. Temporomandibular joints, masticatory and neck muscles were palpated. Helkimo Occlusal, Anamnestic and Clinical Dysfunction Indexes (Index of mandibular movement, index of TMJ function, Index of painful mandibular movements, index of muscular and TMJ pain) were calculated. 3.21% of the subjects were without occlusal disorders (Oi0), 58.32% had moderate occlusal problems (OiI) and 38.56% had severe occlusal problems (OiII). When compared to Anamnestic and Clinical Dysfunction Index the Pearson ?2 test analysis shows that severe occlusal problems are not correlated to severe dysfunction. The difference between the groups is not statistically significant (p > 0.05). The results of this study suggest that occlusion does not have an influence on the occurrence of TMD

Author Correction: Combining CRISPRi and metabolomics for functional annotation of compound libraries

No abstract available

Combining CRISPRi and metabolomics for functional annotation of compound libraries

Molecular profiling of small molecules offers invaluable insights into the function of compounds and allows for hypothesis generation about small-molecule direct targets and secondary effects. However, current profiling methods are limited in either the number of measurable parameters or throughput. Here we developed a multiplexed, unbiased framework that, by linking genetic to drug-induced changes in nearly a thousand metabolites, allows for high-throughput functional annotation of compound libraries in Escherichia coli. First, we generated a reference map of metabolic changes from CRISPR interference (CRISPRi) with 352 genes in all major essential biological processes. Next, on the basis of the comparison of genetic changes with 1,342 drug-induced metabolic changes, we made de novo predictions of compound functionality and revealed antibacterials with unconventional modes of action (MoAs). We show that our framework, combining dynamic gene silencing with metabolomics, can be adapted as a general strategy for comprehensive high-throughput analysis of compound functionality from bacteria to human cell lines

The Relationship Between Occlusion and Temporomadibular Disorders

Etiologija temporomandibularnih poremećaja i može bitini utjecaj nisu potpuno razjašnjeni. Svrha istraživanja bila je utvrditi utjecaj okluzijskih odnosa na funkciju stomatognatoga sustava. Ispitivana skupina sastojala se je od 96 ispitanika u dobi od 24-52 godine (x = 35,03 ± 6,92). Kliničkim pregledom utvrđen je broj zuba, broj zuba u okluziji, okluzijska koncepcija (okluzija vođena očnjakom, grupna funkcija i bilateralno uravnotežena okluzija). Izjereni su iznosi okomitog preklopa i vodoravnog pregriza. RKP položaj određen je chin-point tehnikom i fiksiran Luciajigom. Klizanje iz RKP u IKP položaj izmjereno je u anteroposteriornom, okomitom i postraničnome smjeru. Mjerenja maksimalnih kretnji izvršena su s pomoću precizne pomične mjerke. Registrirana je možebitna pojava zvuka i boli. Izvršena je palpacija temporomandibularnih zglobova, žvačnih i vratnih mišića. Izračunani su Helkimov okuzijski, anamnestiči i klinički disfunkcijski indeks (indeks mandibularne pokretljivosti, indeks funkcije temporomandibularnoga zgloba, indeks bolnosti mandibularnih kretnji te indeks mišićne i zglobne boli). 3,21% ispitanika bilo je bez okluzijskih poremećaja (Oi0), 52,32% imalo je umjerene okluzijske poremećaje (OiI), a 38,56% imalo je ozbiljne okluzijske poremećaje (OiII). U usporedbi s anamnestičkim i kliničkim disfunkcijskim indeksom Pearson ?2 test pokazuje da ozbiljni okluzijski poremećaji nisu povezani sa ozbiljnim disfunkcijskim poremećajima. Razlika među skupinama nije statistički znatna (p > 0,05). Rezultati ovoga istraživanja upućuju na zaključak da okluzija nema utjecaja na nastanak i tijek TMD-a.The etiology of temporomandibular disorders and the role of occlusion, has still not been entirely clarified. The objective of this investigation was to determine the effect of occlusal relationship on the function of the stomatognathic system. A group of subjects, aged from 24-52 years (x = 35.03 ± 6.92) was examined. The number of teeth, the number of teeth in occlusion, type of occlusion (canine guided occlusion, group function and balanced occlusion) was determined by clinical examination. Overbite and overjet were measured. RCP position was determined by chinpoint technique and fixing by Lucia-jig. The sliding from RCP to IKP position was determined in the anteroposterior, vertical and lateral direction. A precise calliper was used to measure maximal jaw movement. Pain and sounds were registred. Temporomandibular joints, masticatory and neck muscles were palpated. Helkimo Occlusal, Anamnestic and Clinical Dysfunction Indexes (Index of mandibular movement, index of TMJ function, Index of painful mandibular movements, index of muscular and TMJ pain) were calculated. 3.21% of the subjects were without occlusal disorders (Oi0), 58.32% had moderate occlusal problems (OiI) and 38.56% had severe occlusal problems (OiII). When compared to Anamnestic and Clinical Dysfunction Index the Pearson ?2 test analysis shows that severe occlusal problems are not correlated to severe dysfunction. The difference between the groups is not statistically significant (p > 0.05). The results of this study suggest that occlusion does not have an influence on the occurrence of TMD

Optimization of a high work function solution processed vanadium oxide hole-extracting layer for small molecule and polymer organic photovoltaic cells

We report a method of fabricating a high work function, solution processable vanadium oxide (V2Ox(sol)) hole-extracting layer. The atmospheric processing conditions of film preparation have a critical influence on the electronic structure and stoichiometry of the V2Ox(sol), with a direct impact on organic photovoltaic (OPV) cell performance. Combined Kelvin probe (KP) and ultraviolet photoemission spectroscopy (UPS) measurements reveal a high work function, n-type character for the thin films, analogous to previously reported thermally evaporated transition metal oxides. Additional states within the band gap of V2Ox(sol) are observed in the UPS spectra and are demonstrated using X-ray photoelectron spectroscopy (XPS) to be due to the substoichiometric nature of V2Ox(sol). The optimized V2Ox(sol) layer performance is compared directly to bare indium–tin oxide (ITO), poly(ethyleneoxythiophene):poly(styrenesulfonate) (PEDOT:PSS), and thermally evaporated molybdenum oxide (MoOx) interfaces in both small molecule/fullerene and polymer/fullerene structures. OPV cells incorporating V2Ox(sol) are reported to achieve favorable initial cell performance and cell stability attributes

Genome-wide association analysis on normal hearing function identifies PCDH20 and SLC28A3 as candidates for hearing function and loss

Hearing loss and individual differences in normal hearing both have a substantial genetic basis. Although many new genes contributing to deafness have been identified, very little is known about genes/variants modulating the normal range of hearing ability. To fill this gap, we performed a two-stage meta-analysis on hearing thresholds (tested at 0.25, 0.5, 1, 2, 4, 8 kHz) and on pure-tone averages (low-, medium-and high-frequency thresholds grouped) in several isolated populations from Italy and Central Asia (total N = 2636). Here, we detected two genome-wide significant loci close to PCDH20 and SLC28A3 (top hits: rs78043697, P = 4.71E-10 and rs7032430, P = 2.39E-09, respectively). For both loci, we sought replication in two independent cohorts: B58C from the UK (N = 5892) and FITSA from Finland (N = 270). Both loci were successfully replicated at a nominal level of significance (P <0.05). In order to confirm our quantitative findings, we carried out RT-PCR and reported RNA-Seq data, which showed that both genes are expressed in mouse inner ear, especially in hair cells, further suggesting them as good candidates for modulatory genes in the auditory system. Sequencing data revealed no functional variants in the coding region of PCDH20 or SLC28A3, suggesting that variation in regulatory sequences may affect expression. Overall, these results contribute to a better understanding of the complex mechanisms underlying human hearing function.Peer reviewe