Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion

The identification of recent HIV infection is important for epidemiological studies and to monitor the epidemic. The objective of this study was to evaluate two rapid tests that are easily available to the Brazilian scientific community for using as markers of recent HIV infection. The Rapid Test - HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brazil) and the Rapid Check HIV 1&2 (NDI-UFES, Center for Infectious Diseases, Universidade Federal do Espirito Santo) were tested, using 489 samples with HIV positive serology, from blood donors, previously classified as recent or long-term infection by serological testing algorithm for recent HIV seroconversion (STARHS) or LS-HIV Vitros assay methods. The samples were diluted prior to testing (1:50 and 1:100 for the Rapid Test - HIV-1/2 Bio-Manguinhos, and 1:500 and 1:600 for the Rapid Check HIV 1&2). Negative samples were considered recent infection, whereas those showing any color intensity were associated with long-term infection. The best dilutions were 1:100 for HIV-1/2 Bio-Manguinhos test (Kappa = 0.840; overall agreement = 0.93), and 1:500 for the Rapid Check HIV 1&2 (Kappa = 0.867; overall agreement = 0.94). The results suggest that both rapid tests can be used to detect recent seroconversion. (C) 2012 Elsevier Editora Ltda. All rights reserved.Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES

Detection recent seroconversion for Immunodeficiency Virus (HIV) though rapid tests modification

Os marcadores utilizados para determinar a infeccao recente pelo HIV sao ferramentas importantes utilizadas no acompanhamento de estudos epidemiologicos da AIDS. objetivo deste estudo foi avaliar dois testes rapidos para HIV u Teste Rapido u HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brasil) e Rapid Check HIV 1&2 (NDI-UFES, Nucleo de Doencas Infecciosas, Universidade Federal do Espirito Santo) - que sao de facil acesso a comunidade cientifica brasileira, para o uso como marcadores de infeccao recente para o HIV. Utilizamos 489 amostras de soro de doadores de sangue com sorologia positiva para o HIV, previamente classificadas em infeccao recente ou de longa data pelo metodo STARHS ou LS-HIV Vitros Assay. As amostras foram diluidas antes da realizacao do teste em 1:50 e em 1:100 para o Teste Rapido u HIV-1/2 Bio-Manguinhos, e em 1:500 e em 1:600, para o Rapid Check HIV 1&2. De acordo com o algoritmo de interpretacao utilizado, as amostras negativas determinaram a infeccao recente naqueles doadores, enquanto que aquelas cujos testes apresentaram qualquer intensidade de coloracao foram associadas a infeccoes de longa duracao. As diluicoes que apresentaram melhores resultados foram 1:100 para o Teste Rapido HIV-1/2 Bio-Manguinhos (Kappa = 0,840; concordancia = 0,93) e 1:500 para o Rapid Check HIV 1&2 (Kappa = 0,867; concordancia = 0,94). Nossos resultados sugerem que os testes rapidos para a deteccao do HIV poderao ser utilizados como uma ferramenta para deteccao de soroconversao recenteBV UNIFESP: Teses e dissertaçõe

Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion

The identification of recent HIV infection is important for epidemiological studies and to monitor the epidemic. The objective of this study was to evaluate two rapid tests that are easily available to the Brazilian scientific community for using as markers of recent HIV infection. The Rapid Test - HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brazil) and the Rapid Check HIV 1&2 (NDI-UFES, Center for Infectious Diseases, Universidade Federal do Espírito Santo) were tested, using 489 samples with HIV positive serology, from blood donors, previously classified as recent or long-term infection by serological testing algorithm for recent HIV seroconversion (STARHS) or LS-HIV Vitros assay methods. The samples were diluted prior to testing (1:50 and 1:100 for the Rapid Test - HIV-1/2 Bio-Manguinhos, and 1:500 and 1:600 for the Rapid Check HIV 1&2). Negative samples were considered recent infection, whereas those showing any color intensity were associated with long-term infection. The best dilutions were 1:100 for HIV-1/2 Bio-Manguinhos test (Kappa = 0.840; overall agreement = 0.93), and 1:500 for the Rapid Check HIV 1&2 (Kappa = 0.867; overall agreement = 0.94). The results suggest that both rapid tests can be used to detect recent seroconversion