Establishment and analysis of a reference transcriptome for Spodoptera frugiperda

International audienceBackground Spodoptera frugiperda (Noctuidae) is a major agricultural pest throughout the American continent. The highly polyphagous larvae are frequently devastating crops of importance such as corn, sorghum, cotton and grass. In addition, the Sf9 cell line, widely used in biochemistry for in vitro protein production, is derived from S. frugiperda tissues. Many research groups are using S. frugiperda as a model organism to investigate questions such as plant adaptation, pest behavior or resistance to pesticides.ResultsIn this study, we constructed a reference transcriptome assembly (Sf_TR2012b) of RNA sequences obtained from more than 35 S. frugiperda developmental time-points and tissue samples. We assessed the quality of this reference transcriptome by annotating a ubiquitous gene family - ribosomal proteins - as well as gene families that have a more constrained spatio-temporal expression and are involved in development, immunity and olfaction. We also provide a time-course of expression that we used to characterize the transcriptional regulation of the gene families studied.ConclusionWe conclude that the Sf_TR2012b transcriptome is a valid reference transcriptome. While its reliability decreases for the detection and annotation of genes under strong transcriptional constraint we still recover a fair percentage of tissue-specific transcripts. That allowed us to explore the spatial and temporal expression of genes and to observe that some olfactory receptors are expressed in antennae and palps but also in other non related tissues such as fat bodies. Similarly, we observed an interesting interplay of gene families involved in immunity between fat bodies and antennae

The Science Performance of JWST as Characterized in Commissioning

This paper characterizes the actual science performance of the James Webb Space Telescope (JWST), as determined from the six month commissioning period. We summarize the performance of the spacecraft, telescope, science instruments, and ground system, with an emphasis on differences from pre-launch expectations. Commissioning has made clear that JWST is fully capable of achieving the discoveries for which it was built. Moreover, almost across the board, the science performance of JWST is better than expected; in most cases, JWST will go deeper faster than expected. The telescope and instrument suite have demonstrated the sensitivity, stability, image quality, and spectral range that are necessary to transform our understanding of the cosmos through observations spanning from near-earth asteroids to the most distant galaxies.Comment: 5th version as accepted to PASP; 31 pages, 18 figures; https://iopscience.iop.org/article/10.1088/1538-3873/acb29

The James Webb Space Telescope Mission

Twenty-six years ago a small committee report, building on earlier studies, expounded a compelling and poetic vision for the future of astronomy, calling for an infrared-optimized space telescope with an aperture of at least $4m$. With the support of their governments in the US, Europe, and Canada, 20,000 people realized that vision as the $6.5m$ James Webb Space Telescope. A generation of astronomers will celebrate their accomplishments for the life of the mission, potentially as long as 20 years, and beyond. This report and the scientific discoveries that follow are extended thank-you notes to the 20,000 team members. The telescope is working perfectly, with much better image quality than expected. In this and accompanying papers, we give a brief history, describe the observatory, outline its objectives and current observing program, and discuss the inventions and people who made it possible. We cite detailed reports on the design and the measured performance on orbit.Comment: Accepted by PASP for the special issue on The James Webb Space Telescope Overview, 29 pages, 4 figure

Imprynted at Caen : l'édition de langue anglaise en Normandie, 1490-1789

Girard Alain-René. Imprynted at Caen : l'édition de langue anglaise en Normandie, 1490-1789 . In: Cahier des Annales de Normandie n°26, 1995. Mélanges René Lepelley. pp. 491-504

Le triomphe de "la cuisinière bourgeoise". Livres culinaires, cuisine et société en France aux XVIIe et XVIIIe siècles

Girard Alain. Le triomphe de "la cuisinière bourgeoise". Livres culinaires, cuisine et société en France aux XVIIe et XVIIIe siècles. In: Revue d’histoire moderne et contemporaine, tome 24 N°4, Octobre-décembre 1977. pp. 497-523

Pierre Albin. La querelle franco-allemande. Le «Coup» d'Agadir, origines et développement de la crise de 1911, 1912

Girard Alain. Pierre Albin. La querelle franco-allemande. Le «Coup» d'Agadir, origines et développement de la crise de 1911, 1912. In: Revue d'histoire moderne et contemporaine, tome 18 N°4,1913. pp. 330-331

Pierre Albin. La querelle franco-allemande. Le «Coup» d'Agadir, origines et développement de la crise de 1911, 1912

Girard Alain. Pierre Albin. La querelle franco-allemande. Le «Coup» d'Agadir, origines et développement de la crise de 1911, 1912. In: Revue d'histoire moderne et contemporaine, tome 18 N°4,1913. pp. 330-331

Avant-propos

Bertaux Jean-Jacques, Girard Alain-René, Nortier Michel. Avant-propos. In: Annales de Normandie. Bibliographie normande 1986. 1987. pp. 401-402

Avant-propos

Bertaux Jean-Jacques, Girard Alain-René, Nortier Michel. Avant-propos. In: Annales de Normandie. Bibliographie normande 1986. 1987. pp. 401-402