Synthetic cathinones related fatalities: an update

Synthetic cathinones, more commonly known as "bath salts", are synthetic drugs chemically related to cathinone, a psychostimulant found in the khat plant. They are the first most consumed products among new psychoactive substances, which cause psychostimulant and hallucinogenic effects determining a number of fatalities worldwide.  In this paper, we have systematically reviewed cases of synthetic cathinones-related fatalities analytically confirmed, which have occurred in the last few years.OBJECTIVE: Synthetic cathinones, more commonly known as “bath salts”, are synthetic drugs chemically related to cathinone, a psychostimulant found in the khat plant. They are the first most consumed products among new psychoactive substances, which cause psychostimulant and hallucinogenic effects determining a number of fatalities worldwide. In this paper, we have systematically reviewed cases of synthetic cathinones-related fatalities analytically confirmed, which have occurred in the last few years. MATERIALS AND METHODS: Relevant scientific articles were identified in Medline, Cochrane Central, Scopus, Web of Science and Institutional/ government websites up to November 2017 using the following keywords: synthetic cathinones, mephedrone, methylenedioxypyrovalerone, MDPV, methylone, ethylone, buthylone, fatal intoxication, fatalities and death. RESULTS: In total, 20 citations met the criteria for inclusion, representing several fatal cases with analytically confirmed synthetic cathinones in biological sample/s of the deceased. The death was attributed to hyperthermia, hypertension, cardiac arrest and more in general to the classic serotonin syndrome. Only rarely did the concentration of the parent drug causing fatality overcome the value of 1 mg/L in post-mortem biological fluids. CONCLUSIONS: Abuse of synthetic cathinones still represents a serious public health issue. Systematic clinical studies on both the animal and human model are lacking; therefore, the only available data are from the users who experience the possible hazardous consequences. Analytical methodologies for the identification of parent compounds and eventual metabolites both in ante-mortem and post-mortem cases need to be developed and validated. Analytical data should be shared through different communication platforms with the aim of stopping this serious health threat for drug users

Suitability of miRNA assessment in postmortem interval estimation

OBJECTIVE: The aim of this review was to explore recent pieces of evidence focused on the use of miRNAs for PMI estimation both in humans and animal experiments, with particular interest on the best miRNAs to use as reference/target markers in different tissues or biological fluids. MiRNAs are innovative biomarkers used in clinical and research field: they appear very attractive, being introduced in forensic research scenarios even for PMI estimation.MATERIALS AND METHODS: Data from PubMed and Scopus were analyzed from January 2013 to August 2020. Based on inclusion/exclusion criteria, high-quality articles have been selected to become the subject of this review.RESULTS: A total of 737 papers were found but, after titles/abstracts screening for inclusion criteria and a full-text careful selection, 33 papers were deeply studied. After the exclusion of 19 papers, 15 articles remained. Eight papers dealt with animals (mice/rats), two both with animals and humans (for method validation previously built), while 5 exclusively with humans. Myocardium (6/15) and brain (6/15) were the most studied tissues. respectively in mice/rats and humans. PMI considered was up to 7.5 days in mouse studies and less than 3 days in human models.CONCLUSIONS: Because of their significant stability in both early and long PMI, miRNAs are the cleverest reference markers to be used. Temperature and environmental conditions influence mostly mRNA, while miRNAs are less susceptible to them. The best miRNA to choose depends on its tissue specificity, i.e., miR-9 and miR-125 in brain or miR-1 and miR-133 in skeletal muscle/heart

Introducing database communication technologies for TED replication in multi-domain networks

In multi-domain transport networks, exchange of Traffic Engineering information is required to enable effective end-to-end service provisioning and restoration by efficiently utilizing network resources. So far, several solutions have been proposed by the communication community such as the Hierarchical Path Computation Element (H-PCE) architecture. Using the H-PCE architecture a parent PCE is responsible for inter-domain path computation, while a dedicated child PCE performs intra-domain path computation within each domain. However, this approach can introduce scalability concerns especially under dynamic traffic condition such as during restoration because all path computation procedures are coordinated by the parent PCE and may require the exchange of many control messages. This paper proposes a standard communication among database systems located at the child PCEs, to exchange and share YANG-based Traffic Engineering information in multi-domain networks. By exploiting currently available database technologies, scalable and predictable performance is demonstrated for both replication mechanisms among child PCEs and information retrieval from the stored databases. Thus, this proposal enables the sharing of intra-domain information at each cPCE that can be locally used, upon failure, to speed-up the recovery procedure

A Survey on the Path Computation Element (PCE) Architecture

Quality of Service-enabled applications and services rely on Traffic Engineering-based (TE) Label Switched Paths (LSP) established in core networks and controlled by the GMPLS control plane. Path computation process is crucial to achieve the desired TE objective. Its actual effectiveness depends on a number of factors. Mechanisms utilized to update topology and TE information, as well as the latency between path computation and resource reservation, which is typically distributed, may affect path computation efficiency. Moreover, TE visibility is limited in many network scenarios, such as multi-layer, multi-domain and multi-carrier networks, and it may negatively impact resource utilization. The Internet Engineering Task Force (IETF) has promoted the Path Computation Element (PCE) architecture, proposing a dedicated network entity devoted to path computation process. The PCE represents a flexible instrument to overcome visibility and distributed provisioning inefficiencies. Communications between path computation clients (PCC) and PCEs, realized through the PCE Protocol (PCEP), also enable inter-PCE communications offering an attractive way to perform TE-based path computation among cooperating PCEs in multi-layer/domain scenarios, while preserving scalability and confidentiality. This survey presents the state-of-the-art on the PCE architecture for GMPLS-controlled networks carried out by research and standardization community. In this work, packet (i.e., MPLS-TE and MPLS-TP) and wavelength/spectrum (i.e., WSON and SSON) switching capabilities are the considered technological platforms, in which the PCE is shown to achieve a number of evident benefits

Demonstration of dynamic restoration in segment routing multi-layer SDN networks

Dynamic traffic recovery is designed and validated in a multi-layer network exploiting an SDN-based implementation of Segment Routing. Traffic recovery is locally performed from the node detecting the failure up to the destination node without involving the SDN controller. Experimental results demonstrate recovery time within 50 ms

Structural validation of a realistic wing structure: the RIBES test article

Several experimental test cases are available in literature to study and validate fluid structure interaction methods. They, however, focus the attention mainly on replicating typical cruising aerodynamic conditions forcing the adoption of fully steel made models able to operate with the high loads generated in high speed facilities. This translates in a complete loss of similitude with typical realistic aeronautical wing structures configurations. To reverse this trend, and to better study the aerolastic mechanism from a structural point of view, an aeroelastic measurement campaign was carried within the EU RIBES project. A half wing model for wind tunnel tests was designed and manufactured replicating a typical metallic wing box structure, producing a database of loads, pressure, stress and deformation measurements. In this paper the design, manufacturing and validation activities performed within the RIBES project are described, with a focus on the structural behavior of the test article. All experimental data and numerical models are made freely available to the scientific community

SDN-enabled Latency-Guaranteed Dual Connectivity in 5G RAN

A novel SDN-controlled E-UTRAN interacting with 5G Radio Resource Management (RRM) featuring Dual Connectivity (DC) is proposed and experimentally demonstrated. Radio bearers are dynamically steered to different evolved NodeBs, to guarantee effective per-flow latency performance.This work was partly funded by the project H2020-ICT-2014-1 “Wishful” (645274), and partly by EU H2020 5GTRANSFORMER project (761536)

Axiomatic Design of a Test Artifact for PBF-LM Machine Capability Monitoring †

Powder Bed Fusion Laser Melting (PBF-LM) additive manufacturing technology is expected to have a remarkable impact on the industrial setting, making possible the realization of a metallic component with very complex designs to enhance product performance. However, the industrial use of the PBF-LM system needs a capability monitoring system to ensure product quality. Among the various studies developed, the investigation of methodology for the actual machine capability determination has been faced and still represents an open point. There are multiple authors and institutes proposing different investigation methods, ranging from the realization of samples (ex situ analysis) to installing monitoring devices on the machine (in situ analysis). Compared to other approaches, sample realization allows for assessing how the machine works through specimen analysis, but it is sensitive to the sample design. In this article, we first present an analysis of a well-known test artifact from an Axiomatic Design perspective. Second, based on the customer needs analysis and adjustments with respect to the use of hypothetical additive production lines, a new test artifact with an uncoupled design matrix is introduced. The proposed design has been experimentally tested and characterized using artifact made of Inconel 718 superalloy to evaluate its performance and representativeness in machine capability assessment. The results show an accurate identification of beam offset and scaling factor considering all the building platform positions. In addition, the artifact is characterized by a reduced building time (more than 90% with respect to the reference NIST artifact) and a halved inspection time (from 16 h to 8 h)