Structural validation of a realistic wing structure: the RIBES test article

Several experimental test cases are available in literature to study and validate fluid structure interaction methods. They, however, focus the attention mainly on replicating typical cruising aerodynamic conditions forcing the adoption of fully steel made models able to operate with the high loads generated in high speed facilities. This translates in a complete loss of similitude with typical realistic aeronautical wing structures configurations. To reverse this trend, and to better study the aerolastic mechanism from a structural point of view, an aeroelastic measurement campaign was carried within the EU RIBES project. A half wing model for wind tunnel tests was designed and manufactured replicating a typical metallic wing box structure, producing a database of loads, pressure, stress and deformation measurements. In this paper the design, manufacturing and validation activities performed within the RIBES project are described, with a focus on the structural behavior of the test article. All experimental data and numerical models are made freely available to the scientific community

Insights into a Protein-Nanoparticle System by Paramagnetic Perturbation NMR Spectroscopy

BACKGROUND: The interaction between proteins and nanoparticles is a very relevant subject because of the potential applications in medicine and material science in general. Further interest derives from the amyloidogenic character of the considered protein, \u3b22-microglobulin (\u3b22m), which may be regarded as a paradigmatic system for possible therapeutic strategies. Previous evidence showed in fact that gold nanoparticles (AuNPs) are able to inhibit \u3b22m fibril formation in vitro. METHODS: NMR (Nuclear Magnetic Resonance) and ESR (Electron Spin Resonance) spectroscopy are employed to characterize the paramagnetic perturbation of the extrinsic nitroxide probe Tempol on \u3b22m in the absence and presence of AuNPs to determine the surface accessibility properties and the occurrence of chemical or conformational exchange, based on measurements conducted under magnetization equilibrium and non-equilibrium conditions. RESULTS: The nitroxide perturbation analysis successfully identifies the protein regions where protein-protein or protein-AuNPs interactions hinder accessibility or/and establish exchange contacts. These information give interesting clues to recognize the fibrillation interface of \u3b22m and hypothesize a mechanism for AuNPs fibrillogenesis inhibition. CONCLUSIONS: The presented approach can be advantageously applied to the characterization of the interface in protein-protein and protein-nanoparticles interactions

Death after smoking of fentanyl, 5F-ADB, 5F-MDMB-P7AICA and other synthetic cannabinoids with a bucket bong

Purpose: We report a case of a polydrug user who consumed various synthetic cannabinoids and fentanyl from a transdermal patch via a bucket bong. Toxicological results from postmortem matrices with special focus on synthetic cannabinoids are discussed in terms of their relevance to the death. Methods: The samples were analyzed by toxicological screening procedures involving immunoassays and gas chromatography–mass spectrometry (GC–MS) as well as quantitative analyses by means of GC–MS and high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS). Results: At the autopsy, coronary artery disease and signs of liver congestion were noted, in the absence of acute myocardial ischemic changes. Femoral blood concentrations of fentanyl and pregabalin were 14 ng/mL and 3,200 ng/mL, respectively. In addition, 2.7 ng/mL 5F-ADB and 13 ng/mL 5F-MDMB-P7AICA were detected together with relatively low amounts of 5 other synthetic cannabinoids in cardiac blood. A total number of up to 17 synthetic cannabinoids were detected in kidney, liver, urine and hair. Fentanyl and 5F-ADB were also detected in the water of the bucket bong. Conclusions: The cause of death could be attributed to an acute mixed intoxication by fentanyl and 5F-ADB (both Toxicological Significance Score (TSS) = 3) with a contribution of pregabalin and 5F-MDMB-P7AICA (TSS = 2), in a subject suffering from pre-existing heart damage. The most plausible mechanism of death consists in a respiratory depression. This case report demonstrates that use of opioids in combination with synthetic cannabinoids might be particularly dangerous

Tandem chemiluminescence-flow injection analysis for dimethoate determination

This work was supported by the Ministry of Education and Science of Spain (Project CTM2006-11991) and FEDER funds.Catalá Icardo, M.; López Paz, JL.; Choves Barón, C. (2010). Tandem chemiluminescence-flow injection analysis for dimethoate determination. Luminescence. 25:235-236. https://doi.org/10.1002/bio.1217S2352362

morphological characteristics of amiata donkey reared in tuscany

In this work were studied morphological characteristics and biometric parameters of the Amiata donkey. The Amiata donkey is a local endangered breed and derives from the homonym mountain in Tuscany. This donkey, which was used, over the past, like pack animal in farms and in mines, is now involved in milk production, onotherapy and trekking. The averages and the standard deviations of the biometric parameters and the frequency of the morphological characters of foals, adult females and stallions were calculated. The measures of the adult females were estimated by ANOVA considering three different classes of age: 3-4, 5-6, over6yearsold. 3-4, 5-6, over 6 years old.. The average sizes were: height at withers of 129.8±4.7and125.8±5.6cm,thoraxcircumferenceof145.6±7.8and145.0±7.8cm, 129.8±4.7 and125.8±5.6cm,thoraxcircumferenceof145.6±7.8and145.0±7.8cm, and 125.8±5.6cm,thoraxcircumferenceof145.6±7.8and145.0±7.8cm, 125.8±5.6 cm,thoraxcircumferenceof145.6±7.8and145.0±7.8cm, , thorax circumference of 145.6±7.8and145.0±7.8cm, 145.6±7.8and145.0±7.8cm, and 145.0±7.8cm, 145.0±7.8 cm,, front shank circumference of 18.3±0.8and16.9±1.5cm,forstallionsandadultfemalesrespectively.All 18.3±0.8 and16.9±1.5cm,forstallionsandadultfemalesrespectively.All and 16.9±1.5cm,forstallionsandadultfemalesrespectively.All 16.9±1.5 cm,forstallionsandadultfemalesrespectively.All , for stallions and adult females respectively. All the observed donkeys presented dark grey coat with the shoulder stripe. The zoometric indices of the adult females did not differ between the classes and showed that the somatic adult proportions had al- ready reached in 3-4 years old donkeys. This breed showed the features of the ancestors: the shoulder stripe of the Equus asinus africanus and the legs stripes of the Equus asinus somaliensis

Homicide or suicide? A probabilistic approach for the evaluation of the manner of death in sharp force fatalities

The role of forensic science can be defined as providing relevant opinions to assist investigators and courts of law in answering questions. The Likelihood Ratio (LR) provides a quantitative and logical approach to communicating the strength of expert evidence. We reviewed existing forensic literature on sharp force fatalities, focusing on studies reporting the manner of death and the frequency of some characteristics that are traditionally assessed. Four studies were included, resulting in a database of 173 suicides and 354 homicides. The LR of each of the characteristic under both hypotheses (suicide and homicide) was obtained. Subsequently, the LR was computed in six fatalities with known manner of death, three suicides and three homicides, by multiplying the corresponding LR of each individual characteristic. LR ranged from 115 to 140,250 in suicidal cases and from 9 to 2728 in homicidal cases. Compared to other fields of forensic science where LR is used extensively, the values obtained in our cases of sharp force fatalities is low. However, in forensic pathology there is evidence that is outside the expert's opinion, and it is for the trier of fact, such as the judge or jury, to draw conclusions. Nevertheless, the LR serves as a tool for interpreting and weighing evidence while maintaining the distinct roles of the trier of fact and the expert. To comprehensively apply the LR in the field of sharp force deaths, it will be necessary to standardize the methodology of investigation and data collection in descriptive studies

HighSTEPS. A high strain temperature pèressure and speed apparatus to study earthquake mechanics

We present a state of-the-art biaxial apparatus able to study both earthquake rupture nucleation and propagation at conditions typical of the seismogenic crust. The HighSTEPS, High Strain TEmperature Pressure Speed, apparatus simulates fault deformation in a wide range of slip velocities, i.e., from 10-5m/s to 0.25 m/s. Within this velocity range, it is possible to study, the rate-and-state friction, the fault dynamic weakening, and healing under unique boundary conditions, i.e., normal stress up to 100 MPa, confining pressure up to 100 MPa, pore fluid pressure up to 100 MPa and temperature up to 120 °C. The apparatus consists of a hydraulic system integrated with four linear motors. The hydraulic system allows for the application of normal stress, confining pressure and pore fluid pressure. The main peculiarity of this apparatus is the system of four linear motors that are mounted in series in order to apply shearing velocities up to 0.25 m/s, accelerations up to 10 m/s2 and shear stresses up to 200 MPa. Moreover, both experiments in sliding velocity control or shear stress control on the experimental faults are possible. Preliminary experiments on carbonate and silicate bearing rocks are coherent with the previous literature. The investigation of fault friction under a wide range of velocities, normal stresses, confining pressures and pore fluid pressures will provide insights into the mechanics of earthquakes and reduce the gap between natural and laboratory observations

Mineral nutrients in soil and pea plants after exposition to TiO2 nanoparticles through a biosolid-amended soil

In addition to the benefits derived from nanotechnology, there is also concern about the potential risks of engineered nanoparticles (ENPs) when released into the environment. Their possible accumulation and effects in agricultural soils and Nanonnovation 2018 Conference & Exibition crops are closely linked to food and agriculture safety. Particular attention has been focused on the reuse of biosolids from wastewater treatment plants that are considered a cost-effective practice for the improvement of nutrients and organic matter in agricultural soils and, but also a sink of contaminants such as nanoparticles (NPs). TiO2NPs have a global production of about 10.000 tons/year and it are among the most extensively used ENPs. Moreover, dissimilar or inconclusive results have been reported concerning the impact of TiO2 NPs on the soil-crop system, thus more information regarding their behavior are necessary. This study aimed to evaluate the potential effects of TiO2 NPs (anatase and rutile) and larger particles (bulk) on the availability of soil nutrients and on the nutritional status of Pisum sativum plants, simulating low (80 mg/kg) and high load of TiO2 (800 mg/kg ) in a biosolid-amended soil. Treated soils were analyzed for N, P, K, Ca, Mg, Mn, Fe, Cu, Zn, soil microbial community, and plants grown in laboratory for 30d were analyzed for growth, pigments and mineral nutrition. Results showed that the treatment with TiO2 at macro- and nano-scale significantly reduced the availability of Mn, Fe and P in soils, this last more evident for the NPs treatments. Indeed, the soil bacterial diversity was reduced when the mixture of anatase and rutile were spiked in the biosolid-amended soil at high concentration. Moreover, the pea plants from treated soils showed an imbalance in the mineral nutrition, with reduction in the plant tissues of Mn and K and increase of N. This study pose a reflection on the use of biosolid, which could act as a vehicle for the spread and accumulation of ENPs in agro-ecosystems