Myocardial blood flow regulation in infarcted patients with stress-induced normalization of negative T waves

The correlation of stress-induced normalization of negative T waves (NTW) with regional myocardial blood flow (MBF) regulation and tissue viability remains still dented

Structural and Folding Dynamic Properties of the T70N Variant of Human Lysozyme

Definition of the transition mechanism from the native globular protein into fibrillar polymer was greatly improved by the biochemical and biophysical studies carried out on the two amyloidogenic variants of human lysozyme, I56T and D67H. Here we report thermodynamic and kinetic data on folding as well as structural features of a naturally occurring variant of human lysozyme, T70N, which is present in the British population at an allele frequency of 5% and, according to clinical and histopathological data, is not amyloidogenic. This variant is less stable than the wild-type protein by 3.7 kcal/mol, but more stable than the pathological, amyloidogenic variants. Unfolding kinetics in guanidine are six times faster than in the wild-type, but three and twenty times slower than in the amyloidogenic variants. Enzyme catalytic parameters, such as maximal velocity and affinity, are reduced in comparison to the wild-type. The solution structure, determined by 1H NMR and modeling calculations, exhibits a more compact arrangement at the interface between the beta-sheet domain and the subsequent loop on one side and part of the alpha domain on the other side, compared with the wild-type protein. This is the opposite of the conformational variation shown by the amyloidogenic variant D67H, but it accounts for the reduced stability and catalytic performance of T70N

Implicazioni medico legali delle lesioni muscolari

L'evento lesivo a livello muscolare costituisce uno degli insulti traumatici pi\uf9 ricorrenti e tra le principali cause si annoverano gli incidenti stradali, i traumi domestici, gli infortuni sul lavoro ed i traumi sportivi

Accertamento e valutazione medico legale della sofferenza morale

Nel mese di aprile 2018 un Gruppo di Esperti Specialisti Medico Legali Nazionali identificati dalla Societ\ue0 Italiana di Medicina Legale (SIMLA) si \ue8 riunito a Padova al fine di confrontarsi, alla luce dei contenuti della legge 4.8.17, n. 124, della dottrina medico legale e della recente giurisprudenza di merito, circa gli ambiti di competenza medico legale nell\u2019accertamento e nella valutazione del \u201cDanno non patrimoniale\u201d alla persona ed ha contestualmente esteso un documento di sintesi con particolare riferimento alla sofferenza morale, successivamente approvato dal Direttivo della predetta Societ\ue0, al fine della diffusione su tutto il territorio nazionale

Stress-induced alteration of left ventricular eccentricity: An additional marker of multivessel CAD

BackgroundAbnormal left ventricular (LV) eccentricity index (EI) is a marker of adverse cardiac remodeling. However, the interaction between stress-induced alterations of EI and major cardiac parameters has not been explored. We sought to evaluate the relationship between LV EI and coronary artery disease (CAD) burden in patients submitted to myocardial perfusion imaging (MPI).Methods and resultsThree-hundred and forty-three patients underwent MPI and coronary angiography. LV ejection fraction (EF) and EI were computed from gated stress images as measures of stress-induced functional impairment.One-hundred and thirty-six (40%), 122 (35%), and 85 (25%) patients had normal coronary arteries, single-vessel CAD, and multivessel CAD, respectively. Post-stress EI was lower in patients with multivessel CAD than in those with normal coronary arteries and single-vessel CAD (P=0.001). This relationship was confirmed only in patients undergoing exercise stress test, where a lower post-stress EI predicted the presence of multivessel CAD (P=0.039).ConclusionsPost-stress alterations of LV EI on MPI may unmask the presence of multivessel CAD

Evaluation data about accuracy of cadmium-zinc-telluride imaging in detecting single and multivessel coronary artery disease: Focus on gender differences

The data presented in this article are related to the research article entitled: “Accuracy of cadmium-zinc-telluride imaging in detecting single and multivessel coronary artery disease: is there any gender difference?” (Gimelli et al., 2018).We evaluated gender-related differences in diagnostic accuracy of cadmium-zinc-telluride (CZT) myocardial perfusion imaging (MPI) in detecting single- and multi-vessel coronary artery disease (CAD). We included 1161 consecutive patients with known or suspected coronary artery disease (228, 25% women and 873, 75% men) who had been referred to our laboratory for stress–rest myocardial perfusion imaging (single-day stress–rest low-dose ultrafast protocol). All patients underwent coronary angiography within 30 days; CAD was defined in the presence of a coronary stenosis >70%. Summed stress scores (SSS), summed rest scores (SRS) and summed difference scores (SDS) were obtained. Image quality was graded “good” or better in more than 90% of patients

Reconstruction of the dynamic in a fatal traffic accident with prolonged dragging of the victim

none7noThe post-mortem examination of victims of fatal road crashes and the reconstruction of the dynamic of traffic accidents are fundamental in vehicular homicides, where the degree of negligence/careless of the driver has to be established. Here we present a case in which a pedestrian was dragged along an SUV for 3.5 km, until the vehicle was parked at the house of the driver, arising the suspect of vehicular manslaughter and failure to provide assistance. On the basis of death scene investigation, analysis of video recording from a surveillance camera, post-mortem computerized tomography (PMCT) and complete post-mortem examination, including gross and microscopical findings, the accident was reconstructed as a frontal collision between the right portion of the bumper and the right side of the body of a standing pedestrian, with deformation of the bumper and production of a metal sheet hook; secondary run-over the right feet, cranio-facial trauma against the bodywork of the vehicle and hooking of the right hand of the victim to the metal sheet; prolonged dragging of the pedestrian over the road surface and severe bleeding. A multidisciplinary-multimodal methodology, including PMCT, allowed to assess the cause of death as hemorrhagic shock, suggested that the victim would have likely survived if the vehicle had stopped, and is increasingly recommendable in fatal road accidents.noneGiorgetti, Arianna; Cecchetto, Giovanni; Giraudo, Chiara; Quaia, Emilio; Viero, Alessia; Viel, Guido; Montisci, MassimoGiorgetti, Arianna; Cecchetto, Giovanni; Giraudo, Chiara; Quaia, Emilio; Viero, Alessia; Viel, Guido; Montisci, Massim