Facteurs associés à la détresse psychologique des étudiants: mieux comprendre pour mieux intervenir

PAREA n°PA-2016-006La présente recherche a été subventionnée par le ministère de l'Éducation et de l'Enseignement supérieur dans le cadre du Programme d'aide à la recherche sur l'enseignement et l'apprentissage (PAREA).Comprend des références bibliographiques

Fermières Obsédées et Women With Kitchen Appliances : le collectif comme espace dialogique des pratiques performatives en art actuel et d'une troisième vague féministe au Québec

Nous explorons, dans ce mémoire, les pratiques performatives des Fermières Obsédées (F.O.) et des Women With Kitchen Appliances (WWKA). Les principaux objectifs poursuivis sont d'éclairer les enjeux et les impacts de la forme collective en art, plus particulièrement comme espace de dialogue des pratiques performatives et de la troisième vague féministe; de montrer comment ces pratiques s'inscrivent dans, et participent à l'histoire de l'art féministe; et de souligner leur contribution en tant que stratégies artistiques féministes en art et dans la société civile. Les collectifs F.O. et WWKA s'inscrivent dans la troisième vague féministe au Québec par leurs pratiques collectives qui allient la performance aux questions sociales et politiques. Cette dimension collective agit tel un espace de dialogue dans lequel les artistes investissent de nouvelles formes de (re)présentation des artistes féministes au Québec et, plus largement, des femmes. Par leurs perspectives critiques sur le monde de même que par la collaboration, les artistes nous renseignent sur différents enjeux féministes actuels tout en s'inscrivant au sein de certains débats qui relèvent de la déconstruction des normes de genre, des sexualités et de la critique anticapitaliste. En apportant leur contribution aux diverses stratégies féministes, les F.O. et les WWKA participent au déploiement de l'agentivité des sujets (artistes comme spectateurs et spectatrices), de même qu'à la production de savoirs situés inédits. Dans l'espace public comme dans des lieux plus intimes, les artistes entremêlent dans leurs œuvres différentes qualités formelles et esthétiques telles que le son, la théâtralité et la fête, leur permettant d'entrer en contact avec l'autre. À l'image des groupes affinitaires féministes à travers l'histoire, les F.O. et les WWKA participent à l'histoire de l'art au Québec, et contribuent à la renouveler.\ud ______________________________________________________________________________ \ud MOTS-CLÉS DE L’AUTEUR : Fermières Obsédées, Women With Kitchen Appliances, collectif affinitaire, art et féminisme, pratiques performatives en art actuel

The effect of university–industry collaboration on the scientific impact of publications : the Canadian case, 1980–2005

Previous research on university-industry collaboration in Canada concluded, using mean impact factors as a proxy, that the scientific impact of such research is not inferior to that of university research. Using field-normalized impact factors and citation counts, this paper reexamines the Canadian case. It shows that, when impact factors are field-normalized, university-industry papers are published, on average, in journals with lower impact factors than papers originating from universities only. However, field-normalized citation values reveal the opposite: the average scientific impact of university-industry papers is significantly above that of both university-only papers and industry-only papers. Collaboration with industries is, thus far from detrimental to the scientific impact of university research and even increases it significantly

A novel therapeutic strategy for pancreatic neoplasia using a novel RNAi platform targeting PDX-1

Bi-functional shRNA (bi-shRNA), a novel RNA interference (RNAi) effector platform targeting PDX-1 utilizing a systemic DOTAP-Cholesterol delivery vehicle, was studied in three mouse models of progressive pancreatic neoplasia. Species-specific bi-functional PDX-1 shRNA (bi-shRNAPDX-1) lipoplexes inhibited insulin expression and secretion while also substantially inhibiting proliferation of mouse and human cell lines via disruption of cell cycle proteins in vitro. Three cycles of either bi-shRNA<sup>mousePDX-1</sup> or shRNA<sup>mousePDX-1</sup> lipoplexes administered intravenously prevented death from hyperinsulinemia and hypoglycemia in a lethal insulinoma mouse model. Three cycles of shRNA<sup>mousePDX-1</sup> lipoplexes reversed hyperinsulinemia and hypoglycemia in an immune-competent mouse model of pancreatic neoplasia. Moreover, three cycles of the bi-shRNA<sup>humanPDX-1</sup> lipoplexes resulted in near complete ablation of tumor volume and considerably improved survival in a human PANC-1 implanted SCID-mouse model. Human pancreatic neoplasia specimens also stained strongly for PDX-1 expression. Together, these data support the clinical development of a novel therapeutic strategy using systemic bi-shRNA<sup>PDX-1</sup> lipoplexes against pancreatic neoplasia

Gold nanoparticles prepared by laser ablation in aqueous biocompatible solutions: assessment of safety and biological identity for nanomedicine applications

International audienceGold nanoparticles prepared by laser ablation in aqueous biocompatible solutions: assessment of safety and biological identity for nanomedicine applications Abstract: Due to excellent biocompatibility, chemical stability, and promising optical properties , gold nanoparticles (Au-NPs) are the focus of research and applications in nanomedicine. Au-NPs prepared by laser ablation in aqueous biocompatible solutions present an essentially novel object that is unique in avoiding any residual toxic contaminant. This paper is conceived as the next step in development of laser-ablated Au-NPs for future in vivo applications. The aim of the study was to assess the safety, uptake, and biological behavior of laser-synthesized Au-NPs prepared in water or polymer solutions in human cell lines. Our results showed that laser ablation allows the obtaining of stable and monodisperse Au-NPs in water, polyethylene glycol, and dextran solutions. The three types of Au-NPs were internalized in human cell lines, as shown by transmission electron microscopy. Biocompatibility and safety of Au-NPs were demonstrated by analyzing cell survival and cell morphology. Furthermore, incubation of the three Au-NPs in serum-containing culture medium modified their physicochemical characteristics , such as the size and the charge. The composition of the protein corona adsorbed on Au-NPs was investigated by mass spectrometry. Regarding composition of complement C3 proteins and apolipoproteins, Au-NPs prepared in dextran solution appeared as a promising drug carrier. Altogether, our results revealed the safety of laser-ablated Au-NPs in human cell lines and support their use for theranostic applications

Optimal human papillomavirus vaccination strategies to prevent cervical cancer in low-income and middle-income countries in the context of limited resources: a mathematical modelling analysis

Introduction of human papillomavirus (HPV) vaccination has been slow in low-income and middle-income countries (LMICs) because of resource constraints and worldwide shortage of vaccine supplies. To help inform WHO recommendations, we modelled various HPV vaccination strategies to examine the optimal use of limited vaccine supplies and best allocation of scarce resources in LMICs in the context of the WHO global call to eliminate cervical cancer as a public health problem. Methods In this mathematical modelling analysis, we developed HPV-ADVISE LMIC, a transmission-dynamic model of HPV infection and diseases calibrated to four LMICs: India, Vietnam, Uganda, and Nigeria. For different vaccination strategies that encompassed use of a nine-valent vaccine (or a two-valent or four-valent vaccine assuming high cross-protection), we estimated three outcomes: reduction in the age-standardised rate of cervical cancer, number of doses needed to prevent one case of cervical cancer (NNV; as a measure of efficiency), and the incremental cost-effectiveness ratio (ICER; in 2017 international $per disability-adjusted life-year [DALY] averted). We examined different vaccination strategies by varying the ages of routine HPV vaccination and number of age cohorts vaccinated, the population targeted, and the number of doses used. In our base case, we assumed 100 Findings We predicted that HPV vaccination could lead to cervical cancer elimination in Vietnam, India, and Nigeria, but not in Uganda. Compared with no vaccination, strategies that involved vaccinating girls aged 9-14 years with two doses were predicted to be the most efficient and cost-effective in all four LMICs. NNV ranged from 78 to 381 and ICER ranged from$28 per DALY averted to $1406 per DALY averted depending on the country. The most efficient and cost-effective strategies were routine vaccination of girls aged 14 years, with or without a later switch to routine vaccination of girls aged 9 years, and routine vaccination of girls aged 9 years with a 5-year extended interval between doses and a catch-up programme at age 14 years. Vaccinating boys (aged 9-14 years) or women aged 18 years or older resulted in substantially higher NNVs and ICERs. Interpretation We identified two strategies that could maximise efforts to prevent cervical cancer in LMICs given constraints on vaccine supplies and costs and that would allow a maximum of LMICs to introduce HPV vaccination. Funding World Health Organization, Canadian Institute of Health Research, Fonds de recherche du Québec-Santé, Compute Canada, PATH, and The Bill & Melinda Gates Foundation. Translations For the French and Spanish translations of the abstract see Supplementary Materials section

The 20S proteasome core, active within apoptotic exosome-like vesicles, induces autoantibody production and accelerates rejection

Autoantibodies to components of apoptotic cells, such as anti-perlecan antibodies, contribute to rejection in organ transplant recipients. However, mechanisms of immunization to apoptotic components remain largely uncharacterized. We used large-scale proteomics, with validation by electron microscopy and biochemical methods, to compare the protein profiles of apoptotic bodies and apoptotic exosome-like vesicles, smaller extracellular vesicles released by endothelial cells downstream of caspase-3 activation. We identified apoptotic exosome-like vesicles as a central trigger for production of anti-perlecan antibodies and acceleration of rejection. Unlike apoptotic bodies, apoptotic exosome-like vesicles triggered the production of anti-perlecan antibodies in naïve mice and enhanced anti-perlecan antibody production and allograft inflammation in mice transplanted with an MHC (major histocompatibility complex)–incompatible aortic graft. The 20S proteasome core was active within apoptotic exosome-like vesicles and controlled their immunogenic activity. Finally, we showed that proteasome activity in circulating exosome-like vesicles increased after vascular injury in mice. These findings open new avenues for predicting and controlling maladaptive humoral responses to apoptotic cell components that enhance the risk of rejection after transplantation

New PI(4,5)P2- and membrane proximal integrin–binding motifs in the talin head control β3-integrin clustering

A talin intermolecular interaction autoinhibits its own activation and regulates β3-integrin binding. When bound, β3-integrin undergoes structural alterations that prevent its β and α subunits from associating, maintaining β3-integrin's clustering capability