Contribution of the Twin Arginine Translocation system to the exoproteome of Pseudomonas aeruginosa

The opportunistic pathogen Pseudomonas aeruginosa uses secretion systems to deliver exoproteins into the environment. These exoproteins contribute to bacterial survival, adaptation, and virulence. The Twin arginine translocation (Tat) export system enables the export of folded proteins into the periplasm, some of which can then be further secreted outside the cell. However, the full range of proteins that are conveyed by Tat is unknown, despite the importance of Tat for the adaptability and full virulence of P. aeruginosa. In this work, we explored the P. aeruginosa Tat-dependent exoproteome under phosphate starvation by two-dimensional gel analysis. We identified the major secreted proteins and new Tat-dependent exoproteins. These exoproteins were further analyzed by a combination of in silico analysis, regulation studies, and protein localization. Altogether we reveal that the absence of the Tat system significantly affects the composition of the exoproteome by impairing protein export and affecting gene expression. Notably we discovered three new Tat exoproteins and one novel type II secretion substrate. Our data also allowed the identification of two new start codons highlighting the importance of protein annotation for subcellular predictions. The new exoproteins that we identify may play a significant role in P. aeruginosa pathogenesis, host interaction and niche adaptation

LISA AIVT Optical Ground Support Equipement technology developments

International audienceThe LISA space interferometer aims at GW detection with »3x10-20/√Hz strain sensitivity, resulting in a displacement sensitivity of 11pm/√Hz over a path length of 2.5x109 m in the frequency range from 3x10-5 to 1 Hz.The LISA France Collaboration is in charge of the ground optical tests of the MOSA (Moving Optical Sub-Assembly), including the Optical Bench, Telescope and Gravitational Reference Sensor. Special check-out equipment is required, such as the Far-Field Optical Ground Support Equipment aiming at measuring the Tilt-To-Length coupling coefficient between angular residual beam jitter and longitudinal path length. The FF-OGSE simulates the incoming jittering beam and measures the associated longitudinal path length change.We present two prototypes – the Zerodur InterFerOmeter and the TTL-OB - that will demonstrate the optical performance, the functional tests, the limits on sensitivity and the precision of the path length measurements achievable on-ground. These two benches are the first part of the design and specification for the FF-OGSE.The Stray Light OGSE aims at stray light characterization in the integrated MOSA. It measures and identifies, separately, the different sources of stray light through the measurement of the corresponding fringe patterns while scanning the laser’s optical frequency

LISA AIVT Optical Ground Support Equipement technology developments

International audienceThe LISA space interferometer aims at GW detection with »3x10-20/√Hz strain sensitivity, resulting in a displacement sensitivity of 11pm/√Hz over a path length of 2.5x109 m in the frequency range from 3x10-5 to 1 Hz.The LISA France Collaboration is in charge of the ground optical tests of the MOSA (Moving Optical Sub-Assembly), including the Optical Bench, Telescope and Gravitational Reference Sensor. Special check-out equipment is required, such as the Far-Field Optical Ground Support Equipment aiming at measuring the Tilt-To-Length coupling coefficient between angular residual beam jitter and longitudinal path length. The FF-OGSE simulates the incoming jittering beam and measures the associated longitudinal path length change.We present two prototypes – the Zerodur InterFerOmeter and the TTL-OB - that will demonstrate the optical performance, the functional tests, the limits on sensitivity and the precision of the path length measurements achievable on-ground. These two benches are the first part of the design and specification for the FF-OGSE.The Stray Light OGSE aims at stray light characterization in the integrated MOSA. It measures and identifies, separately, the different sources of stray light through the measurement of the corresponding fringe patterns while scanning the laser’s optical frequency

LISA AIVT Optical Ground Support Equipement technology developments

International audienceThe LISA space interferometer aims at GW detection with »3x10-20/√Hz strain sensitivity, resulting in a displacement sensitivity of 11pm/√Hz over a path length of 2.5x109 m in the frequency range from 3x10-5 to 1 Hz.The LISA France Collaboration is in charge of the ground optical tests of the MOSA (Moving Optical Sub-Assembly), including the Optical Bench, Telescope and Gravitational Reference Sensor. Special check-out equipment is required, such as the Far-Field Optical Ground Support Equipment aiming at measuring the Tilt-To-Length coupling coefficient between angular residual beam jitter and longitudinal path length. The FF-OGSE simulates the incoming jittering beam and measures the associated longitudinal path length change.We present two prototypes – the Zerodur InterFerOmeter and the TTL-OB - that will demonstrate the optical performance, the functional tests, the limits on sensitivity and the precision of the path length measurements achievable on-ground. These two benches are the first part of the design and specification for the FF-OGSE.The Stray Light OGSE aims at stray light characterization in the integrated MOSA. It measures and identifies, separately, the different sources of stray light through the measurement of the corresponding fringe patterns while scanning the laser’s optical frequency

LISA AIVT Optical Ground Support Equipement technology developments

International audienceThe LISA space interferometer aims at GW detection with »3x10-20/√Hz strain sensitivity, resulting in a displacement sensitivity of 11pm/√Hz over a path length of 2.5x109 m in the frequency range from 3x10-5 to 1 Hz.The LISA France Collaboration is in charge of the ground optical tests of the MOSA (Moving Optical Sub-Assembly), including the Optical Bench, Telescope and Gravitational Reference Sensor. Special check-out equipment is required, such as the Far-Field Optical Ground Support Equipment aiming at measuring the Tilt-To-Length coupling coefficient between angular residual beam jitter and longitudinal path length. The FF-OGSE simulates the incoming jittering beam and measures the associated longitudinal path length change.We present two prototypes – the Zerodur InterFerOmeter and the TTL-OB - that will demonstrate the optical performance, the functional tests, the limits on sensitivity and the precision of the path length measurements achievable on-ground. These two benches are the first part of the design and specification for the FF-OGSE.The Stray Light OGSE aims at stray light characterization in the integrated MOSA. It measures and identifies, separately, the different sources of stray light through the measurement of the corresponding fringe patterns while scanning the laser’s optical frequency

LISA AIVT Optical Ground Support Equipement technology developments

International audienceThe LISA space interferometer aims at GW detection with »3x10-20/√Hz strain sensitivity, resulting in a displacement sensitivity of 11pm/√Hz over a path length of 2.5x109 m in the frequency range from 3x10-5 to 1 Hz.The LISA France Collaboration is in charge of the ground optical tests of the MOSA (Moving Optical Sub-Assembly), including the Optical Bench, Telescope and Gravitational Reference Sensor. Special check-out equipment is required, such as the Far-Field Optical Ground Support Equipment aiming at measuring the Tilt-To-Length coupling coefficient between angular residual beam jitter and longitudinal path length. The FF-OGSE simulates the incoming jittering beam and measures the associated longitudinal path length change.We present two prototypes – the Zerodur InterFerOmeter and the TTL-OB - that will demonstrate the optical performance, the functional tests, the limits on sensitivity and the precision of the path length measurements achievable on-ground. These two benches are the first part of the design and specification for the FF-OGSE.The Stray Light OGSE aims at stray light characterization in the integrated MOSA. It measures and identifies, separately, the different sources of stray light through the measurement of the corresponding fringe patterns while scanning the laser’s optical frequency