18 research outputs found
Caamembecaia gratiosus n. gen., n. sp. (Acari: Trombiculidae), from Trinomys gratiosus (Gunter) (Rodentia: Echimydae), of Atlantic Forest in Southeastern Brazil
From June 1999 to May 2001, small mammals were captured in three areas of the Atlantic Forest in Southeastern Brazil and examined for ectoparasites. Analysis of ectoparasites revealed the presence of a new chigger genus and species, Caamembecaia gratiosus, from Trinomys gratiosus. This is the first record of a chigger from T. gratiosus.13713
One World, One Health Challenge: The Holistic Understanding of Rickettsiosis Integrating Multi-Criteria Analysis Techniques and Spatial Statistics
Among the human diseases caused by etiological agents transmitted by ticks in Brazil, the most prevalent and public health interest is that one caused by species of RickettsiaâSpotted Fever (SF). We applied the concept âOne World, One Healthâ to achieve a proper understanding of SF and determine risk scenarios for human infection by pathogenic Rickettsia in the state of Rio de Janeiro (RJ). Multi-criteria decision analysis and spatial statistics were performed on data encompassing epidemiology, health care, biotic determents, and socioeconomic and demographic variables. The construction of multi-criteria descriptors used 33 indicators ordered in 12 sub criteria of 5 major categories: public health, environmental health, acarology, veterinary health, and microbiology. SF happened nonrandomly in RJ and the risk was heterogeneous in the weight of indicators contribution mainly associated with the criteria acarology (35.11%), microbiology (33.25%), and veterinary medicine (23.96%). Spatially, and according to the PROMETHEE algorithm, ticks from Amblyomma sculptum class and dogs are determinants for the occurrence of human cases of SF in RJ. To define indicators of SF and plan health actions, the âOne World, One Healthâ concept proved to be applicable at three levels: (i) the local or possible areas of infection, (ii) the population or municipality, and (iii) the ecosystem or state. The model of study is flexible according to the reality of the endemic areas and also demonstrates its applicability from a national to a local (home) scale
Anaplasmataceae in cats (Felis catus) in the city of Campos dos Goytacazes, Rio de Janeiro
No Brasil, atĂ© o ano 2000, os agentes riquetsiais em felinos domĂ©sticos eram poucos conhecidos, existindo somente relatos esporĂĄdicos de Ehrlichia sp. As recentes pesquisas envolvendo biologia molecular e agentes riquetsiais confirmam a ideia de que estes agentes estĂŁo presentes nesses animais e, por este motivo, demonstram a necessidade de estudos mais detalhados no Brasil. O objetivo do presente trabalho foi a caracterização dos agentes da famĂlia Anaplasmataceae que acometem os felinos domĂ©sticos e esclarecer a importĂąncia dos felinos na cadeia epidemiolĂłgica das doenças riquetsiais por mĂ©todos moleculares e sorolĂłgicos associando a presença das doenças aos parĂąmetros clĂnicos e laboratoriais. Foram obtidas amostras sanguĂneas de 60 felinos domĂ©sticos, independentes de sanidade, provenientes de atendimentos clĂnicos. Destas amostras foram realizados hemograma e bioquĂmica sĂ©rica, e os dados foram utilizados para preenchimento da ficha laboratorial. As amostras foram processadas para obtenção de concentração de cĂ©lulas e soro, para realização da reação em cadeia pela polimerase (PCR) e reação por imunofluorescĂȘncia indireta (RIFI), respectivamente, para identificação de agentes da famĂlia Anaplasmataceae. Os dados foram utilizados para anĂĄlise descritiva para formação de frequĂȘncias epidemiolĂłgicas e para realização de testes nĂŁo-paramĂ©tricos pelo Qui-quadrado de Pearson (pâ€5%) associando as alteraçÔes laboratoriais Ă s infecçÔes por Ehrlichia canis, Anaplasma platys e Anaplasma phagocytophilum. Os resultados obtidos revelaram a presença de 33,33% de agentes Anaplamastaceae na amostra populacional, sendo 8,33% para E. canis, 20% para A. platys e 10% para A. phagocytophilum. Foram realizadas as sorologias das amostras, pela imunofluorescĂȘncia indireta, para verificação de amostras reagentes para A. phagocytophilum, sendo 8,33% amostras reagentes na amostra populacional. As alteraçÔes clĂnicas e laboratoriais mais frequentes em pacientes positivos por agentes Anaplasmataceae foram letargia, linfadenomegalia, mucosas pĂĄlidas, desidratação, trombocitopenia, hiperglobulinemia e hipoalbuminemia. Destes dados foram realizadas as correlaçÔes nĂŁo paramĂ©tricas e nĂŁo foram verificadas dependĂȘncias das alteraçÔes laboratoriais com a presença de animais positivos para agentes Anaplasmataceae. A identificação dos agentes E. canis e A. platys visa esclarecer a doença na regiĂŁo, sendo instrumento de orientação da doença pelo mĂ©dico veterinĂĄrio ao proprietĂĄrio para que tenha medidas adequadas de tratamento e prevenção. A presença de agentes A. phagocytophilum Ă© considerada, sem dĂșvidas, uma notificação importante devido ao potencial zoonĂłtico.In Brazil, by the year 2000, rickettsioses in domestic cats were little known and there were only sporadic reports of Ehrlichia sp. Recent research involving molecular biology and rickettsioses confirm the notion of the presence of theses agents in cats and show the need for more studies in Brazil. The objective of this paper was to characterize agents belonging to the Anaplasmataceae family that affect domestic cats and to clarify the importance of cats in the epidemiology of rickettsioses by molecular and serological methods associating the presence of disease with clinical and laboratory parameters. Blood samples were obtained from 60 healthy domestic cats. Blood count and serum biochemical tests were performed, and the data were registered. The samples were processed to obtain cell concentration and serum to perform the polymerase chain reaction (PCR) and the indirect immunofluorescence assay (IFA) respectively, in order to identify agents of the Anaplasmataceae family. The data were used for descriptive analysis to obtain frequencies and to perform non-parametric tests with the chi-square test (pâ€5%), besides the laboratory findings of infection by Ehrlichia canis, Anaplasma phagocytophilum and Anaplasma platys. The results revealed that 33.33% of the agents belonged to the Anaplasmataceae family, 8.33% for E. canis, 20% for A. platys, and 10% for A. phagocytophilum. Serology samples were examined by indirect immunofluorescence to check samples reacting to A. phagocytophilum, with positive reaction of 8.33%. The most frequent clinical and laboratory findings in patients positive for Anaplasmataceae agents were lethargy, enlargement of lymph nodes, pale mucous membranes, dehydration, thrombocytopenia, hyperglobulinemia and hypoalbuminemia. These data had non-parametric correlation and the laboratory changes and presence of positive cats was not interdependent. Identification of E. canis and A. platys revealed the disease in the region of Campos dos Goytacazes/RJ. The presence of A. phagocytophilum is considered an important finding due to its zoonotic potential
ManifestaçÔes dermatolĂłgicas desencadeadas por ĂĄcaros da famĂlia Cheyletidae: relato de caso
Internal setae number of the larvae Hallerâs organ of Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae) from Silva Jardim and ItaguaĂ, RJ
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Previous issue date: 2005Conselho Nacional de Desenvolvimento CientĂfico e TecnolĂłgico. Programa Institucional de Bolsas de Iniciação CientĂfica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ, Brasil.Larvae of Amblyomma cajennense (Fabricius, 1787), were obtained under laboratory conditions. The larvae were killed in hot water, preserved in 70o ethanol and prepared in definitive whole mounts. Two hundred larvae were examined under brightfield microscopy with an immersion objective in order to analyze the variation of the number of internal setae of the right and left side of the Hallerâs organ chamber with the purpose of utilize them as chaetotaxy parameter for specific diagnosis
Two new species of the genus Geckobia MĂ©gnin, 1878 (Acariformes, Prostigmata, Pterygosomatidae) from Peru
Machado, Ingrid B., GazĂȘta, Gilberto S., PĂ©rez, JosĂ©, Cunha, Rodolfo, Giupponi, Alessandro P. De L. (2019): Two new species of the genus Geckobia MĂ©gnin, 1878 (Acariformes, Prostigmata, Pterygosomatidae) from Peru. Zootaxa 4657 (2): 333-351, DOI: 10.11646/zootaxa.4657.2.
Frequency of Equine Monocytic Ehrlichiosis (EME) in Brazil
From a cross-sectional observational study with convenience samples, 347 blood samples from horses were collected from different physiographic regions, as follows: Santa Catarina Plateau (Santa Catarina State - SC), MĂ©dio ParaĂba do Sul (SĂŁo Paulo State - SP and Rio de Janeiro State RJ), Mountainous and Metropolitan regions (Rio de Janeiro State - RJ). Samples were tested for the presence of antibodies (IgG) anti Neorickettsia risticii by indirect immunofluorescence assay (IFA). The frequency obtained in this study corroborates with the ones obtained in the U.S.A., which refers to endemic regions. Fisher's exact test showed significant differences in the number of positive animals between regions, indicating that the probability of an animal becoming infected varies depending on the area. The CI 95% revealed no association between infection and geopolitical space. Moreover, Odds ratio test showed differences of an animal getting infected in different regions. This event could be influenced by the type of treatment used in each area, as the seasonal frequency of injury or even potential vectors. Therefore, there are seropositive animals for N. risticii in the studied areas, suggesting that this agent may be circulating in those regions. Future studies mainly based on molecular analyzes are needed to confirm these serological findings
Caamembecaia gratiosus n. gen., n. sp. (Acari: Trombiculidae), from Trinomys gratiosus (Gunter) (Rodentia: Echimydae), of Atlantic Forest in Southeastern Brazil
From June 1999 to May 2001, small mammals were captured in three areas
of the Atlantic Forest in Southeastern Brazil and examined for
ectoparasites. Analysis of ectoparasites revealed the presence of a new
chigger genus and species, Caamembecaia gratiosus , from
Trinomys gratiosus . This is the first record of a chigger from
T. gratiosus