Caamembecaia gratiosus n. gen., n. sp. (Acari: Trombiculidae), from Trinomys gratiosus (Gunter) (Rodentia: Echimydae), of Atlantic Forest in Southeastern Brazil

From June 1999 to May 2001, small mammals were captured in three areas of the Atlantic Forest in Southeastern Brazil and examined for ectoparasites. Analysis of ectoparasites revealed the presence of a new chigger genus and species, Caamembecaia gratiosus, from Trinomys gratiosus. This is the first record of a chigger from T. gratiosus.13713

One World, One Health Challenge: The Holistic Understanding of Rickettsiosis Integrating Multi-Criteria Analysis Techniques and Spatial Statistics

Among the human diseases caused by etiological agents transmitted by ticks in Brazil, the most prevalent and public health interest is that one caused by species of Rickettsia—Spotted Fever (SF). We applied the concept “One World, One Health” to achieve a proper understanding of SF and determine risk scenarios for human infection by pathogenic Rickettsia in the state of Rio de Janeiro (RJ). Multi-criteria decision analysis and spatial statistics were performed on data encompassing epidemiology, health care, biotic determents, and socioeconomic and demographic variables. The construction of multi-criteria descriptors used 33 indicators ordered in 12 sub criteria of 5 major categories: public health, environmental health, acarology, veterinary health, and microbiology. SF happened nonrandomly in RJ and the risk was heterogeneous in the weight of indicators contribution mainly associated with the criteria acarology (35.11%), microbiology (33.25%), and veterinary medicine (23.96%). Spatially, and according to the PROMETHEE algorithm, ticks from Amblyomma sculptum class and dogs are determinants for the occurrence of human cases of SF in RJ. To define indicators of SF and plan health actions, the “One World, One Health” concept proved to be applicable at three levels: (i) the local or possible areas of infection, (ii) the population or municipality, and (iii) the ecosystem or state. The model of study is flexible according to the reality of the endemic areas and also demonstrates its applicability from a national to a local (home) scale

Anaplasmataceae in cats (Felis catus) in the city of Campos dos Goytacazes, Rio de Janeiro

No Brasil, até o ano 2000, os agentes riquetsiais em felinos domésticos eram poucos conhecidos, existindo somente relatos esporádicos de Ehrlichia sp. As recentes pesquisas envolvendo biologia molecular e agentes riquetsiais confirmam a ideia de que estes agentes estão presentes nesses animais e, por este motivo, demonstram a necessidade de estudos mais detalhados no Brasil. O objetivo do presente trabalho foi a caracterização dos agentes da família Anaplasmataceae que acometem os felinos domésticos e esclarecer a importância dos felinos na cadeia epidemiológica das doenças riquetsiais por métodos moleculares e sorológicos associando a presença das doenças aos parâmetros clínicos e laboratoriais. Foram obtidas amostras sanguíneas de 60 felinos domésticos, independentes de sanidade, provenientes de atendimentos clínicos. Destas amostras foram realizados hemograma e bioquímica sérica, e os dados foram utilizados para preenchimento da ficha laboratorial. As amostras foram processadas para obtenção de concentração de células e soro, para realização da reação em cadeia pela polimerase (PCR) e reação por imunofluorescência indireta (RIFI), respectivamente, para identificação de agentes da família Anaplasmataceae. Os dados foram utilizados para análise descritiva para formação de frequências epidemiológicas e para realização de testes não-paramétricos pelo Qui-quadrado de Pearson (p≤5%) associando as alterações laboratoriais às infecções por Ehrlichia canis, Anaplasma platys e Anaplasma phagocytophilum. Os resultados obtidos revelaram a presença de 33,33% de agentes Anaplamastaceae na amostra populacional, sendo 8,33% para E. canis, 20% para A. platys e 10% para A. phagocytophilum. Foram realizadas as sorologias das amostras, pela imunofluorescência indireta, para verificação de amostras reagentes para A. phagocytophilum, sendo 8,33% amostras reagentes na amostra populacional. As alterações clínicas e laboratoriais mais frequentes em pacientes positivos por agentes Anaplasmataceae foram letargia, linfadenomegalia, mucosas pálidas, desidratação, trombocitopenia, hiperglobulinemia e hipoalbuminemia. Destes dados foram realizadas as correlações não paramétricas e não foram verificadas dependências das alterações laboratoriais com a presença de animais positivos para agentes Anaplasmataceae. A identificação dos agentes E. canis e A. platys visa esclarecer a doença na região, sendo instrumento de orientação da doença pelo médico veterinário ao proprietário para que tenha medidas adequadas de tratamento e prevenção. A presença de agentes A. phagocytophilum é considerada, sem dúvidas, uma notificação importante devido ao potencial zoonótico.In Brazil, by the year 2000, rickettsioses in domestic cats were little known and there were only sporadic reports of Ehrlichia sp. Recent research involving molecular biology and rickettsioses confirm the notion of the presence of theses agents in cats and show the need for more studies in Brazil. The objective of this paper was to characterize agents belonging to the Anaplasmataceae family that affect domestic cats and to clarify the importance of cats in the epidemiology of rickettsioses by molecular and serological methods associating the presence of disease with clinical and laboratory parameters. Blood samples were obtained from 60 healthy domestic cats. Blood count and serum biochemical tests were performed, and the data were registered. The samples were processed to obtain cell concentration and serum to perform the polymerase chain reaction (PCR) and the indirect immunofluorescence assay (IFA) respectively, in order to identify agents of the Anaplasmataceae family. The data were used for descriptive analysis to obtain frequencies and to perform non-parametric tests with the chi-square test (p≤5%), besides the laboratory findings of infection by Ehrlichia canis, Anaplasma phagocytophilum and Anaplasma platys. The results revealed that 33.33% of the agents belonged to the Anaplasmataceae family, 8.33% for E. canis, 20% for A. platys, and 10% for A. phagocytophilum. Serology samples were examined by indirect immunofluorescence to check samples reacting to A. phagocytophilum, with positive reaction of 8.33%. The most frequent clinical and laboratory findings in patients positive for Anaplasmataceae agents were lethargy, enlargement of lymph nodes, pale mucous membranes, dehydration, thrombocytopenia, hyperglobulinemia and hypoalbuminemia. These data had non-parametric correlation and the laboratory changes and presence of positive cats was not interdependent. Identification of E. canis and A. platys revealed the disease in the region of Campos dos Goytacazes/RJ. The presence of A. phagocytophilum is considered an important finding due to its zoonotic potential

Internal setae number of the larvae Haller’s organ of Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae) from Silva Jardim and Itaguaí, RJ

Submitted by Sandra Infurna ([email protected]) on 2019-05-30T16:14:45Z No. of bitstreams: 1 GilbertoGazeta_NMSerraFreire_etal_IOC_2005.pdf: 145277 bytes, checksum: ad5e4b6fa6f90a86ce611107aa0033ff (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-05-30T16:20:22Z (GMT) No. of bitstreams: 1 GilbertoGazeta_NMSerraFreire_etal_IOC_2005.pdf: 145277 bytes, checksum: ad5e4b6fa6f90a86ce611107aa0033ff (MD5)Made available in DSpace on 2019-05-30T16:20:22Z (GMT). No. of bitstreams: 1 GilbertoGazeta_NMSerraFreire_etal_IOC_2005.pdf: 145277 bytes, checksum: ad5e4b6fa6f90a86ce611107aa0033ff (MD5) Previous issue date: 2005Conselho Nacional de Desenvolvimento Científico e Tecnológico. Programa Institucional de Bolsas de Iniciação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ, Brasil.Larvae of Amblyomma cajennense (Fabricius, 1787), were obtained under laboratory conditions. The larvae were killed in hot water, preserved in 70o ethanol and prepared in definitive whole mounts. Two hundred larvae were examined under brightfield microscopy with an immersion objective in order to analyze the variation of the number of internal setae of the right and left side of the Haller’s organ chamber with the purpose of utilize them as chaetotaxy parameter for specific diagnosis

Two new species of the genus Geckobia Mégnin, 1878 (Acariformes, Prostigmata, Pterygosomatidae) from Peru

Machado, Ingrid B., Gazêta, Gilberto S., Pérez, José, Cunha, Rodolfo, Giupponi, Alessandro P. De L. (2019): Two new species of the genus Geckobia Mégnin, 1878 (Acariformes, Prostigmata, Pterygosomatidae) from Peru. Zootaxa 4657 (2): 333-351, DOI: 10.11646/zootaxa.4657.2.

Frequency of Equine Monocytic Ehrlichiosis (EME) in Brazil

From a cross-sectional observational study with convenience samples, 347 blood samples from horses were collected from different physiographic regions, as follows: Santa Catarina Plateau (Santa Catarina State - SC), Médio Paraíba do Sul (São Paulo State - SP and Rio de Janeiro State RJ), Mountainous and Metropolitan regions (Rio de Janeiro State - RJ). Samples were tested for the presence of antibodies (IgG) anti Neorickettsia risticii by indirect immunofluorescence assay (IFA). The frequency obtained in this study corroborates with the ones obtained in the U.S.A., which refers to endemic regions. Fisher's exact test showed significant differences in the number of positive animals between regions, indicating that the probability of an animal becoming infected varies depending on the area. The CI 95% revealed no association between infection and geopolitical space. Moreover, Odds ratio test showed differences of an animal getting infected in different regions. This event could be influenced by the type of treatment used in each area, as the seasonal frequency of injury or even potential vectors. Therefore, there are seropositive animals for N. risticii in the studied areas, suggesting that this agent may be circulating in those regions. Future studies mainly based on molecular analyzes are needed to confirm these serological findings

Caamembecaia gratiosus n. gen., n. sp. (Acari: Trombiculidae), from Trinomys gratiosus (Gunter) (Rodentia: Echimydae), of Atlantic Forest in Southeastern Brazil

From June 1999 to May 2001, small mammals were captured in three areas of the Atlantic Forest in Southeastern Brazil and examined for ectoparasites. Analysis of ectoparasites revealed the presence of a new chigger genus and species, Caamembecaia gratiosus , from Trinomys gratiosus . This is the first record of a chigger from T. gratiosus