Sustainable Design in Fair Trade Shops. A project to increase the environmental awarness of consumers

The subject of this study is to define the possible strategies in order to reduce the environmental impact of designing store and to increase the consumers' awareness about the new sustainable design in fair trade shops. From the analysis of some crucial elements in the store at environmental level, the weaknesses and the strengthens of fair trade store sector are underlined. All of them are examined in order to define the concept and guidelines for the executive project. The resulting concept brings to the showcase many reusable local materials and background poster that tells the products' place of origin and their history to embodied the ecodesign principles and to educate costumers on sustainability principles. The products are places above shelves made by abandoned fruit boxes already wasted in the store, matched labels that will be re-used as business card. This research defined a project able to give answer to technical and marketing store's requirements, but also to environmental sustainability needs

GreenTeam. A new educative approach to sustainable design

Sustainability, meaning its three pillars, social, economic and environmental, is by now a prerequisite in the design phase and one of the mandatory topics in educational programs, both for future designers and other professions. This happens even more if the project theme concerns mobility or waste management, fields in which daily behaviours impact on the system, the ones adopted by teenagers as well. Is it possible and correct to use an academic teaching methodology, based on participation and learner-teacher mutual exchange, that usually works with adult and motivated people, with a group of high school students? If the actors of the system already adopt sustainable behaviours for contingent reasons and lack of resources rather than the real will and awareness, what are the actions to be taken? The paper answers these questions through the analysis of a direct experience of the authors: GreenTeam

Effects of NSAIDs on the Release of Calcitonin Gene-Related Peptide and Prostaglandin E2 from Rat Trigeminal Ganglia

Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used to treat migraine, but the mechanisms of their effects in this pathology are not fully elucidated. The trigeminal ganglia and calcitonin gene-related peptide (CGRP) have been implicated in the pathophysiology of migraine. The release of CGRP and prostaglandin E 2 (PGE 2 ) from freshly isolated rat trigeminal ganglia was evaluated after oral administration of nimesulide, etoricoxib, and ketoprofen, NSAIDs with different pharmacological features. Thirty minutes after oral administration, nimesulide, 10 mg/Kg, decreased the GCRP release induced by an inflammatory soup, while the other NSAIDs were ineffective at this point in time. Two hours after oral nimesulide (5 and 10 mg/Kg) and ketoprofen (10 mg/Kg), but not of etoricoxib, a significant decrease in the CGRP release was observed. All drugs reduced PGE 2 , although with some differences in timing and doses, and the action on CGRP does not seem to be related to PGE 2 inhibition. The reduction of CGRP release from rat trigeminal ganglia after nimesulide and ketoprofen may help to explain the mechanism of action of NSAIDs in migraine. Since at 30 minutes only nimesulide was effective in reducing CGRP release, these results suggest that this NSAID may exert a particularly rapid effect in patients with migraine

Antagonism of the prokineticin system prevents and reverses allodynia and inflammation in a mouse model of diabetes

Neuropathic pain is a severe diabetes complication and its treatment is not satisfactory. It is associated with neuroinflammation-related events that participate in pain generation and chronicization. Prokineticins are a new family of chemokines that has emerged as critical players in immune system, inflammation and pain. We investigated the role of prokineticins and their receptors as modulators of neuropathic pain and inflammatory responses in experimental diabetes. In streptozotocin-induced-diabetes in mice, the time course expression of prokineticin and its receptors was evaluated in spinal cord and sciatic nerves, and correlated with mechanical allodynia. Spinal cord and sciatic nerve pro- and anti-inflammatory cytokines were measured as protein and mRNA, and spinal cord GluR subunits expression studied. The effect of preventive and therapeutic treatment with the prokineticin receptor antagonist PC1 on behavioural and biochemical parameters was evaluated. Peripheral immune activation was assessed measuring macrophage and T-helper cytokine production. An up-regulation of the Prokineticin system was present in spinal cord and nerves of diabetic mice, and correlated with allodynia. Therapeutic PC1 reversed allodynia while preventive treatment blocked its development. PC1 normalized prokineticin levels and prevented the up-regulation of GluN2B subunits in the spinal cord. The antagonist restored the pro-/anti-inflammatory cytokine balance altered in spinal cord and nerves and also reduced peripheral immune system activation in diabetic mice, decreasing macrophage proinflammatory cytokines and the T-helper 1 phenotype. The prokineticin system contributes to altered sensitivity in diabetic neuropathy and its inhibition blocked both allodynia and inflammatory events underlying disease

New Technological Approach for the Evaluation of Postural Control Abilities in Children with Developmental Coordination Disorder

Background: Developmental Coordination Disorder (DCD) causes difficulties in postural control which are crucial to assess due to their impact on everyday life. There is a lack of suitable tools to acquire quantitative data and deeply analyze postural control, especially during the developmental age. The aim of this study is to investigate postural control skills in children with DCD and typically developing children (TD) using the Virtual Reality Rehabilitation System (VRRS). Methods: 18 children with DCD and 30 TD children (mean age 9.12 +/- 2.65 and 7.12 +/- 2.77 years, respectively) were tested by using the Movement Assessment Battery for Children Second Edition (MABC-2) and a VRRS stabilometric balance platform. A t-test was performed to identify differences in the VRRS parameters between the two groups. Furthermore, we investigated whether a correlation exists between the VRRS data and the MABC-2. Results: Significant differences (p < 0.05) in mean distance and frequency of the COP are found in the two groups. These parameters also correlate with the MABC-2 total score (p <= 0.05) and balance subscales (p <= 0.05). Conclusions: This study opens a new frontier for the assessment of postural skills in children with DCD and represents a potential basis for a tailored rehabilitation program, from which their postural stability and, consequently, their everyday life will benefit

Two-compartment perfusion MR IVIM model to investigate normal and pathological placental tissue

Background: Perfusion and diffusion coexist in the placenta and can be altered by pathologies. The two-perfusion model, where f1 and, f2 are the perfusion-fraction of the fastest and slowest perfusion compartment, respectively, and D is the diffusion coefficient, may help differentiate between normal and impaired placentas. Purpose: Investigate the potential of the two-perfusion IVIM model in differentiating between normal and abnormal placentas. Study-Type: Retrospective, case–control. Population: 43 normal pregnancy, 9 fetal-growth-restriction (FGR), 6 small-for-gestational-age (SGA), 4 accreta, 1 increta and 2 percreta placentas. Field Strength/Sequence: Diffusion-weighted-echo planar imaging sequence at 1.5 T. Assessment: Voxel-wise signal-correction and fitting-controls were used to avoid overfitting obtaining that two-perfusion model fitted the observed data better than the IVIM model (Akaike weight: 0.94). The two-perfusion parametric-maps were quantified from ROIs in the fetal and maternal placenta and in the accretion zone of accreta placentas. The diffusion coefficient D was evaluated using a b ≥ 200 sec/mm2-mono-exponential decay fit. IVIM metrics were quantified to fix f1 + f2 = fIVIM. Statistical-Tests: ANOVA with Dunn-Sidák's post-hoc correction and Cohen's d test were used to compare parameters between groups. Spearman's coefficient was evaluated to study the correlation between variables. A P-value<0.05 indicated a statistically significant difference. Results: There was a significant difference in f1 between FGR and SGA, and significant differences in f2 and fIVIM between normal and FGR. The percreta + increta group showed the highest f1 values (Cohen's d = −2.66). The f2 between normal and percreta + increta groups showed Cohen's d = 1.12. Conversely, fIVIM had a small effective size (Cohen's d = 0.32). In the accretion zone, a significant correlation was found between f2 and GA (ρ = 0.90) whereas a significant negative correlation was found between fIVIM and D (ρ = −0.37 in fetal and ρ = −0.56 in maternal side) and f2 and D (ρ = −0.38 in fetal and ρ = −0.51 in maternal side) in normal placentas. Conclusion: The two-perfusion model provides complementary information to IVIM parameters that may be useful in identifying placenta impairment. Level of Evidence: 2. Technical Efficacy Stage: 1

Vocal behavior and the use of social information during roost finding

When selecting feeding, hiding, or resting areas, animals face multiple decisions with different fitness consequences. To maximize efficiency, individuals can either collect personal information, or use information gathered and transmitted by other individuals (social information). Within group living species, organisms often specialize in either generating social information or using information gathered by other groups members. That is the case of the Spix’s disk-winged bat, Thyroptera tricolor. This species uses contact calls during roost finding. Social groups are composed by a mix of vocal and non-vocal individuals and those vocal roles appear to be consistent over time. Moreover, their vocal behavior can predict roost finding in natural settings, suggesting that vocal individuals are capable of generating social information that can be used by other group members. To date, however, we do not know if when presented with social information (contact calls) during roost finding, vocal individuals will make more or less use of these cues, compared to non-vocal individuals. To answer this question, we broadcast contact calls from a roost inside a flight cage to test whether vocal individuals could find a potential roost faster than non-vocal individuals when they encounter sounds that signal the presence of a roost site. Our results suggest that non-vocal individuals select roost sites based primarily on social information, whereas vocal individuals do not rely heavily on social information when deciding where to roost. This study provides the first link between vocal behavior and the use of social information during the search for roosting resources in bats. Incorporating ideas of social roles, and how individuals decide when and where to move based on the use of social information, may shed some light on these and other outstanding questions about the social lives of bats.Universidad de Costa Rica/[560-B8-015]/UCR/Costa RicaUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de BiologíaUCR::Sedes Regionales::Sede del Su

Molecular and cellular studies reveal folding defects of human ornithine aminotransferase variants associated with gyrate atrophy of the choroid and retina

The deficit of human ornithine aminotransferase (hOAT) is responsible for gyrate atrophy (GA), a rare recessive inherited disorder. Although more than 60 disease-associated mutations have been identified to date, the molecular mechanisms explaining how each mutation leads to the deficit of OAT are mostly unknown. To fill this gap, we considered six representative missense mutations present in homozygous patients concerning residues spread over the hOAT structure. E. coli expression, spectroscopic, kinetic and bioinformatic analyses, reveal that the R154L and G237D mutations induce a catalytic more than a folding defect, the Q90E and R271K mutations mainly impact folding efficiency, while the E318K and C394Y mutations give rise to both folding and catalytic defects. In a human cellular model of disease folding-defective variants, although at a different extent, display reduced protein levels and/or specific activity, due to increased aggregation and/or degradation propensity. The supplementation with Vitamin B6, to mimic a treatment strategy available for GA patients, does not significantly improve the expression/activity of folding-defective variants, in contrast with the clinical responsiveness of patients bearing the E318K mutation. Thus, we speculate that the action of vitamin B6 could be also independent of hOAT. Overall, these data represent a further effort toward a comprehensive analysis of GA pathogenesis at molecular and cellular level, with important relapses for the improvement of genotype/phenotype correlations and the development of novel treatments