An Investigation on filtration rate of bivalve Dreissena polymorpha in different temperatures in Anzali Lagoon

Filtration rate of bivalve Dreissena polymorpha with two treatments and four replicates and two controls was assessed in Anzali Lagoon laboratory. Results showed that the mean filtration rate of bivalve on alga Chlorella volgaris in temperatures 1-6'c and 25°c are 125m1 per hour and 58.65ml per hour respectively. There was a significant difference between mean filtration rates with the two treatments (P<0.05)

Annual cycle of ovarian development and sex hormones of grey mullet (Mugil cephalus) in captivity

The grey mullet fingerling were imported to Iran in 1997 from Hong Kong and in coastal fish pond of north part of Iran were successfully cultured in order to obtain broodstocks and induce artificial reproduction. Seasonal changes in serum concentrations of Testosterone, 17-β Estradiole (E2), 17-α Hydroxy Progesterone and also the level of calcium, Triglyceride and cholesterol in females grey mullet in captivity were measured by Radioimmunoassay or colorimetry, respectively. Investigating of sex steroids in different sexual maturity indicated that increasing of oocyte diameter and sexual maturity development serum testosterone was increased significantly (P0.05). These results suggest that the seasonal changes in serum lipids and gonadal steroids were associated with reproductive development. The data also support the hypothesis that the shortening photoperiod is a major factor in stimulating reproductive activity in striped mullet

Growth, survival and stress resistance of tiger barb (Puntius tetrazona) larvae fed on linseed oil-enriched Artemia franciscana nauplii

Effect of feeding on linseed oil (LO)-enriched Artemia was investigated on growth, survival and stress resistance of tiger barb (Puntius tetrazona) larvae. Larvae were fed by LO-enriched (2.5, 5 and 7.5%) as well as non-enriched Artemia nauplii for 14 d, followed by 14 d feeding on non-enriched Artemia. Fish fed on enriched nauplii showed significant increase in growth performance at both 14th and 28th d. Also, fish fed on enriched Artemia, especially those fed on 7.5% LO, showed greater resistance in response to osmotic and hypoxia stress at 28th d. The results suggest that LO enriched Artemia nauplii are capable to promote growth and stress response in tiger barb larvae

Evaluation of effects of physico-chemical factors on chlorophyll-a in Shadegan International Wetland - Khouzestan Province - Iran

Measurement of chlorophyll-a can be considered important to investigate the primary production of an ecosystem. This study aimed to investigate chlorophyll-a concentration, primary production and the factors affecting them in Shadegan Wetland located in Khouzestan Province - Iran. Sampling for measuring (NO3, PO4, BOD5, DO, pH, EC, salinity, temperature) was performed seasonally at five sampling stations of the wetland (Canal, Ragbeh, Khoroosi, Mahshahr and Atish), from March 2013 – through February 2014. Results showed that chlorophyll-a concentrations in Canal station were significantly higher than that in the other stations; however, there were no significant differences among the other sampling stations (p>0.05). The maximum and minimum primary production (and chlorophyll-a) (62, 1.14 mg/m^3) were observed at Canal and Ragbeh stations, respectively. Annual average chlorophyll-a concentration was 10.28 mg/m^3, and there was no significant seasonal difference (p>0.05). The maximum value of chlorophyll-a was observed in the fall (29.63 mg/m^3), whereas, the minimum value was related to the spring (4.07 mg/m^3). Among the water physicochemical parameters, nitrate had a significant effect on chlorophyll-a concentrations. According to trophic state index (TSI), Shadegan Wetland is mesotrophic in the spring and winter, whereas it is eutrophic in the summer and fall. Overall, the lake is suitable for warm water species

An investigation on the growth and survival of Rutilus frissi kutum in Geomembrane Tanks

An investigation was carried out on the sustainability and growth rate of Rutilus frissi kutum larvae in polyethylene tanks in Ghazian Aquaculture Research Station, Guilan Province. The results were compared to those recorded for the currently used tanks including fiberglass and glass aquariums. Three treatments each with three replications were designed and the tanks were stocked with 30 fishes, with an average initial weight of 650 to 746 mg. The tanks were filled with 70 liters of water and the experiment was conducted for 60 days. The fish were fed with concentrated food during the rearing period. The fish showed an increase rate of 625.88, 643.54 and 659.48 mg in fiberglass, aquarium and polyethylene tanks, respectively. No significant difference was observed in the indices special growth ratio (SGR), food conversion ratio (FCR) and weight gain (P>0.05). The research indicated that the high-density polyethylene tanks made are more suitable for R. frissi kutum culture than fiberglass and aquarium tanks

Topical application of silymarin enhances cutaneous wound healing in rats

Abstract Wound healing in a short period with minimum side effects is one of the major goals of medical sciences. Silymarin, an extract from Silybum marianum, has been shown to have antioxidant and anti-inflammatory properties. This study investigates the wound healing activity of silymarin topical formulation in an in experimental model. A 875 mm2 (25 × 35 mm) full-thickness excision was made on the abdominal region of each rat by a surgical blade and the day on which the wound was made considered as day 0. Each rat was treated two times each day. On days 1,4, 8 and 12, the wound area was measured using precise caliber and camera imaging. On day 12, blood samples were collected for the analysis of antioxidant, malondialdehyde and estradiol levels. After 12 days of treatment, rats were sacrificed and abdominal region tissues used for histological analyses. The study showed that topical application of silymarin on wound in rats improved wound healing correlating with less redness, exudates and swelling. Furthermore, in serum of rats treated with silymarin ointment improved antioxidant and estradiol levels, while decreased malondialdehyde levels, a marker of oxidative stress. Histological analyses showed also an improve of novel blood vessels. This effect on angiogenesis correlated with improve nitric oxide synthase expression and epithelial cells after treatment with silymarin. Silymarin ointment represents a promising therapeutic agent for the treatment of wounds through its antioxidant and anti-inflammatory properties

Clinical characteristics and outcomes of pregnant women with COVID-19 and comparison with control patients: A systematic review and meta-analysis

In a large-scale study, 128176 non-pregnant patients (228 studies) and 10000 pregnant patients (121 studies) confirmed COVID-19 cases included in this Meta-Analysis. The mean (confidence interval CI) of age and gestational age of admission (GA) in pregnant women was 33 (28�37) years old and 36 (34�37) weeks, respectively. Pregnant women show the same manifestations of COVID-19 as non-pregnant adult patients. Fever (pregnant: 75.5%; non-pregnant: 74%) and cough (pregnant: 48.5%; non-pregnant: 53.5%) are the most common symptoms in both groups followed by myalgia (26.5%) and chill (25%) in pregnant and dysgeusia (27%) and fatigue (26.5%) in non-pregnant patients. Pregnant women are less probable to show cough (odds ratio OR 0.7; 95% CI 0.67�0.75), fatigue (OR: 0.58; CI: 0.54�0.61), sore throat (OR: 0.66; CI: 0.61�0.7), headache (OR: 0.55; CI: 0.55�0.58) and diarrhea (OR: 0.46; CI: 0.4�0.51) than non-pregnant adult patients. The most common imaging found in pregnant women is ground-glass opacity (57%) and in non-pregnant patients is consolidation (76%). Pregnant women have higher proportion of leukocytosis (27% vs. 14%), thrombocytopenia (18% vs. 12.5%) and have lower proportion of raised C-reactive protein (52% vs. 81%) compared with non-pregnant patients. Leucopenia and lymphopenia are almost the same in both groups. The most common comorbidity in pregnant patients is diabetes (18%) and in non-pregnant patients is hypertension (21%). Case fatality rate (CFR) of non-pregnant hospitalized patients is 6.4% (4.4�8.5), and mortality due to all-cause for pregnant patients is 11.3% (9.6�13.3). Regarding the complications of pregnancy, postpartum hemorrhage (54.5% 7�94), caesarean delivery (48% 42�54), preterm labor (25% 4�74) and preterm birth (21% 12�34) are in turn the most prevalent complications. Comparing the pregnancy outcomes show that caesarean delivery (OR: 3; CI: 2�5), low birth weight (LBW) (OR: 9; CI: 2.4�30) and preterm birth (OR: 2.5; CI: 1.5�3.5) are more probable in pregnant woman with COVID-19 than pregnant women without COVID-19. The most prevalent neonatal complications are neonatal intensive care unit admission (43% 2�96), fetal distress (30% 12�58) and LBW (25% 16�37). The rate of vertical transmission is 5.3% (1.3�16), and the rate of positive SARS-CoV-2 test for neonates born to mothers with COVID-19 is 8% (4�16). Overall, pregnant patients present with the similar clinical characteristics of COVID-19 when compared with the general population, but they may be more asymptomatic. Higher odds of caesarean delivery, LBW and preterm birth among pregnant patients with COVID-19 suggest a possible association between COVID-19 infection and pregnancy complications. Low risk of vertical transmission is present, and SARS-CoV-2 can be detected in all conception products, particularly placenta and breast milk. Interpretations of these results should be done cautiously due to the heterogeneity between studies; however, we believe our findings can guide the prenatal and postnatal considerations for COVID-19 pregnant patients. © 2020 John Wiley & Sons Ltd

Risk of stroke in hospitalized SARS-CoV-2 infected patients: A multinational study

Background: There is an increased attention to stroke following SARS-CoV-2. The goal of this study was to better depict the short-term risk of stroke and its associated factors among SARS-CoV-2 hospitalized patients. Methods: This multicentre, multinational observational study includes hospitalized SARS-CoV-2 patients from North and South America (United States, Canada, and Brazil), Europe (Greece, Italy, Finland, and Turkey), Asia (Lebanon, Iran, and India), and Oceania (New Zealand). The outcome was the risk of subsequent stroke. Centres were included by non-probability sampling. The counts and clinical characteristics including laboratory findings and imaging of the patients with and without a subsequent stroke were recorded according to a predefined protocol. Quality, risk of bias, and heterogeneity assessments were conducted according to ROBINS-E and Cochrane Q-test. The risk of subsequent stroke was estimated through meta-analyses with random effect models. Bivariate logistic regression was used to determine the parameters with predictive outcome value. The study was reported according to the STROBE, MOOSE, and EQUATOR guidelines. Findings: We received data from 26,175 hospitalized SARS-CoV-2 patients from 99 tertiary centres in 65 regions of 11 countries until May 1st, 2020. A total of 17,799 patients were included in meta-analyses. Among them, 156(0.9) patients had a stroke�123(79) ischaemic stroke, 27(17) intracerebral/subarachnoid hemorrhage, and 6(4) cerebral sinus thrombosis. Subsequent stroke risks calculated with meta-analyses, under low to moderate heterogeneity, were 0.5 among all centres in all countries, and 0.7 among countries with higher health expenditures. The need for mechanical ventilation (OR: 1.9, 95 CI:1.1�3.5, p = 0.03) and the presence of ischaemic heart disease (OR: 2.5, 95 CI:1.4�4.7, p = 0.006) were predictive of stroke. Interpretation: The results of this multi-national study on hospitalized patients with SARS-CoV-2 infection indicated an overall stroke risk of 0.5(pooled risk: 0.9). The need for mechanical ventilation and the history of ischaemic heart disease are the independent predictors of stroke among SARS-CoV-2 patients. Funding: None. © 2020 The Author

Supply and protected different population of Litopenaeus vannamei subadult zero foster (F0) from difference provinces Iran

Nowadays, Litopenaeus vannamei are the most important species of farmed penaeidae shrimp in the world that is rapidly replacing native species in areas aquaculture. Due to demand increase for this species culture, shrimp displacement to different areas may be associated with some potential pathogens transferred to new areas farmed. Therefore, in this study were prepared bi-osecurity conditions for specific disease-free production of L. vannamei. Thereafter, three populations (Molokaei, High health and mix of Molokaei and High health) of the shrimp various reserves were detected base on origin and genetic indexes such as: observed heterozygosity, expected heterozygosity, allele frequency, coefficient inbreeding, genetic differentiation, genetic distance and genetic identity. On the other hand, epidemiological studies indicate non pathogens (viral, bacterial, fungal and parasitic) recognition of different populations selected in the quarantine salon. The bioassay results showed that the average weight and length of the populations of High health and Mix significantly greater than was a population of Molokaei. The shrimp populations were stocked in fiberglass tank (five ton) and were kept separated in the quarantine salon. During maintenance shrimp of populations in the quarantine salon were evaluated living and non-living pathogens with PCR, microbiology and biochemical methods. There is not any pathogens detection from shrimp populations stocking in the quarantine salon, so the shrimps were carried over to pond for broodstock culture of specific pathogenic free

Caspian brown trout (Salmo trutta caspius) broodstock supply in south western of the Caspian Sea (Guilan province)

Caspian trout (Salmo trutta caspius ) is one of the migratory fish species which lives and feeds in the Caspian Sea .This species distributed in south and western of the Caspian Sea . In order to rehabilitation of the Caspian trout in western area, the operation of the project started from the beginning of the fishing season of the 20112012. Specimens of the Caspian trout obtained from beach seine cooperatives and have been transferred to the fish farm in Asalem region .Totally, 44 specimens of the Caspian trout has been caught in two month period .Most of the fishes was from Kargan roud river . The mean length and weight of the brood fishes were 67.7 cm and 3122.7 g respectively .As at present Shilat uses just the broods of the Tonekaboon region for restocking of this species ,we recommend to use the broods of the western region separately for rehabilitation of the stocks of this region