Defense mechanisms and Somatization: The effect of defense hierarchy on somatization

هدف تحقیق حاضر بررسی نقش سبک‌های دفاعی غالب در پیش‌بینی علائم جسمانی‌سازی و وضعیت سلامت عمومی است. نمونه این پژوهش شامل 253 دانشجو (149 زن و 104 مرد) بوده‌است و ابزارهای مورد استفاده پرسشنامه سبک‌های دفاعی اندروز (1993)، چک ‌لیست علائم جسمانی‌سازی ریف و هیلر (1998) و پرسشنامه سلامت عمومی گلدبرگ (1979) بودند. نتایج نشان دادند که سبک‌های دفاعی، پیش‌بین مناسبی برای علائم جسمانی‌سازی و سلامت عمومی در افراد هستند. بدین‌صورت که سبک دفاعی رشدیافته، علائم جسمانی‌سازی را به­صورت منفی و سلامت عمومی را به­صورت مثبت پیش‌بینی می‌کرد. همچنین سبک‌های دفاعی رشدنایافته و نوروتیک به ترتیب علائم جسمانی‌سازی را به­طور مثبت و سلامت عمومی را به­شکل منفی پیش‌بینی می‌کردند. علاوه ‌بر این، دیگر یافته‌ها نشان دادند که به­طور اختصاصی دفاع‌‌های خیال‌پردازی اوتیستیک، برون‌ریزی، انزوا و ابطال به­ترتیب پیش‌بینی کننده مثبت علائم جسمانی‌سازی در افراد بودند. یافته‌های این مطالعه می‌تواند شاهدی بر نقش احتمالی سبک‌های‌ دفاعی در ایجاد و عود اختلالات روان‌تنی محسوب شود. روان‌درمانگران می‌توانند با تمرکز بر این سبک‌های دفاعی، باعث کاهش جسمانی‌سازی و ارتقای سلامت عمومی گردند

Inhibitory effect of gold nanoparticles conjugated with interferon gamma and methionine on breast cancer cell line

AbstractObjectiveTo develop a gold nanoparticles complex conjugated with interferon-gamma (IFN-γ) and methionine along with application of hyperthermia using near-infrared laser beams for the treatment of cancer cells.MethodsGold nanorods (10 nm) were conjugated with IFN-γ and methionine using carbodiimide family and characterized after purification by dialysis bags. Breast cancer cells were cultured and incubated with gold nanorods at different concentrations followed by irradiation with near-infrared laser beam. Samples were then evaluated for their viability in order to determine the effect of treatment and variables by MTT assy.ResultsZetasizer results confirmed the conjugation of gold nanorods with methionine and IFN-γ. The median percentage of cell viability in 0.30 μg/mL concentration of gold nanorods was 82%. The cell viability reached to 85% at the same concentration of gold nanorods, which existed in the assayed complex. The results of MTT assay showed that the 0.60 μg/mL concentration of gold nanoparticles complex was toxic on tumor cells (P < 0.05). After exposure to hyperthermia, the viability of cells at 6 min decreased to 77% in 0.30 μg/mL concentration of gold nanorods complex.ConclusionsThe size and concentration of gold nanorods was not cytotoxic. However, their presence during irradiation near-infrared laser increased the number of dead cells during the treatment of cells

Determination and comparison rate of expression markers of osteoblast derived of Adipose derived stem cells markers in monolayer and pellet culture models

Abstract: Nowadays high accident rates, fractures leading to permanent bone disorders and the impossibility of bone transplant have made scientists to look for new methods of repairing injured bones. Considering the application of stem cells in bone tissue engineering, there exists the necessity to investigate various culture methods and suitable fields and scaffolds. Thus, we decided to induce adipose-derived stem cells into osteoblast cells in two systems of pellet culture and monolayer and compare osteogenic markers. Methods: Stem cells have been separated via mechanical and enzymatic methods and cultured in monolayer and pellet culture models with osteogenic medium. Then, RNA was separated from differentiated cells, complementary DNA (cDNA) was synthesized and amplified. Polymerase chain reaction (PCR) product was transferred to electrophoresis gel. The intensity of the bands was measured by Image-J software and analyzed by SPSS. Results: average osteopontin, osteocalcin and Runx2 genes in differentiated cells in the two culture systems showed a significant difference. The expression of osteocalcin, osteopontin and Runx2 gense in pellet system were more than monolayer systems in 21 days. Conclusion: This study indicated that pellet and monolayer culture systems are appropriate for bone engineering but osteocalcin, osteopontin and Runx2 genes expressions were different in the two culture system

Formation of the Chah-Gaz iron oxide-apatite ore (IOA) deposit, Bafq District, Iran: constraints from halogens, trace element concentrations, and Sr-Nd isotopes of fluorapatite

The textures, chemistry and Sr-Nd isotopic compositions of apatite from the Chah-Gaz iron-oxide apatite (IOA) deposit in the Bafq metallogenic belt, central Iran, were studied to investigate the formation of this ore deposit. Two generations of apatite were recognized based on Cl/F and Cl/OH ratios. Primary fluorapatite, which is coeval with magnetite in the massive ore bodies, is chemically homogeneous and characterized by Cl/F < 0.05 and Cl/OH in the range of 0.01–0.07. By contrast, F-depleted apatite rims are present in apatite hosted in veinlets that crosscut the massive ore bodies and are disseminated in the igneous host rocks, and have Cl/F and Cl/OH-apatite ratios of 0.08–0.12 and 0.15–0.79, respectively. The F-depleted rims are also depleted in LREEs, Th and U, consistent with the presence of secondary monazite, xenotime and thorite that formed by coupled dissolution-reprecipitation. The whole-rock Nd–Sr isotopic data ((87Sr/86Sr)(I) = 0.7052 to 0.7064 and ɛNd(t) = +1.3 to + 2.7) of gabbro-diorite indicate an mantle source, while the high -K, calc-alkaline-shoshonitic volcanic host rocks have εNd(t) = − 5.5 to − 7.6, clearly reflecting mixing between mantle-derived mafic magmas and assimilated Proterozoic basement. The initial 87Sr/86Sr ratios and ɛNd values of both F-rich and -depleted apatites (0.7038 to 0.7050 and −0.3 to + 6.5, respectively) are similar to gabbroic rocks and support a magmatic source for primary fluorapatite, with minimal or no crustal contribution, and indicate that the magmatic event with negative ɛNd values did not affect the whole-rock Sm-Nd signature of the ore. Petrography plus geochemical and Nd-Sr isotopic data of both studied fluorapatite, which come from iron oxide stage, are consistent with a combined igneous/magmatic-hydrothermal genesis for the Chah-Gaz IOA deposit, with low degrees of hydrothermal overprint, as evidenced by the formation of F-depleted (Cl-rich) apatite rims. The relatively constant Sr-Nd data in apatite are consistent with superimposed, episodic hydrothermal fluids from the same, evolving, magmatic-hydrothermal system

Multi-Scale Computational Modeling of Two-Phased Metal Using GMC Method

A multi-scale computational model for determining plastic behavior in two-phased CMSX-4 Ni-based superalloys is developed on a finite element analysis (FEA) framework employing crystal plasticity constitutive model that can capture the microstructural scale stress field. The generalized method of cells (GMC) micromechanics model is used for homogenizing the local field quantities. At first, GMC as stand-alone is validated by analyzing a repeating unit cell (RUC) as a two-phased sample with 72.9% volume fraction of gamma'-precipitate in the gamma-matrix phase and comparing the results with those predicted by finite element analysis (FEA) models incorporating the same crystal plasticity constitutive model. The global stress-strain behavior and the local field quantity distributions predicted by GMC demonstrated good agreement with FEA. High computational saving, at the expense of some accuracy in the components of local tensor field quantities, was obtained with GMC. Finally, the capability of the developed multi-scale model linking FEA and GMC to solve real life sized structures is demonstrated by analyzing an engine disc component and determining the microstructural scale details of the field quantities

Association between Objectively measured Physical Activity and Sedentary Behavior with Body Composition among Primary School Children

Background: Physical activity (PA) plays a crucial role in the overall well-being of children in the long term. The present study aimed to investigate the correlation between PA, sedentary behavior (SB), and body composition (BC) among primary school children.Methods: This descriptive-correlational study was conducted in Ali-Abad-Katoul, Iran in 2023. A sample size of 322 boys was selected using convenience sampling method. ActiGraph wGT3X-BT accelerometer and Body Composition X-Scan Plus II were used for measuring PA pattern and BC. Pearson correlation and Independent t tests were used to analyze the data.Results: The accelerometer data revealed that participants spent 67.55% of the total time in SB. Moreover, on average, daily moderate-to-vigorous physical activity (MVPA) was 49.71±22.37 minutes. Accordingly, the daily energy expenditure was 558.39±250.17 kcal. SB was directly and significantly associated with Body Mass Index (BMI) (r=0.628, P<0.001) and Mass of Body Fat (MBF) (r=0.347, P<0.001). In addition, our results revealed an inverse and significant correlation between MVPA with BMI (r=-0.849, P<0.001) and MBF (r=-0.716, P<0.001). Finally, the children who did meet the MVPA guideline had significantly lower BMI (t=-3.781, P<0.001) and MBF (t=4.892, P<0.001) and higher Skeletal Muscle Mass (SMM) (t=4.209, P<0.001) than those who did not meet the MVPA guideline.Conclusions: These findings suggested that vigorous PA and SB play a role in controlling children’s adiposity, highlighting the importance of engaging children in high-intensity PA and reducing SB

Grain Size-Dependent Crystal Plasticity Constitutive Model for Polycrystal Materials

A new method to introduce grain size-dependence in classical crystal plasticity constitutive model is developed by considering the resistance to dislocation motion in the grain boundary influence region as equivalent to that of a work hardening. A general framework for the size-dependent constitutive model is derived by implementing this method on a core and mantle model. The work hardening, equivalent to grain boundary effect, is realized by introducing a fictitious, pre-existing, plastic strain grain boundary influence region (mantle) following the principles of classical crystal plasticity. This fictitious plastic strain, in effect, increases the yield strength and decreases the initial hardening coefficient of the grain. With the thickness of grain boundary influence region and the distribution of introduced plastic strain remaining the same, the grain boundary effect increased as the grain size becomes smaller. A simplified model that considers the grain boundary effect on a grain average sense is also developed under this general framework. Implementation of this general framework to the specific cases of crystal plasticity constitutive models is demonstrated by considering the case of power law flow rule and hyperbolic-secant hardening rule. Finally, the grain size-dependent constitutive model is validated by comparing the predicted stress-strain behavior of polycrystal samples with different average grain sizes under uniaxial loading with the experimental results