37 research outputs found
Functional shift with maintained regenerative potential following portal vein ligation
Selective portal vein ligation (PVL) allows the two-stage surgical resection of primarily unresectable liver tumours by generating the atrophy and hypertrophy of portally ligated (LL) and non-ligated lobes (NLL), respectively. To evaluate critically important underlying functional alterations, present study characterised in vitro and vivo liver function in male Wistar rats (n = 106; 210-250 g) before, and 24/48/72/168/336 h after PVL. Lobe weights and volumes by magnetic resonance imaging confirmed the atrophy-hypertrophy complex. Proper expression and localization of key liver transporters (Ntcp, Bsep) and tight junction protein ZO-1 in isolated hepatocytes demonstrated constantly present viable and well-polarised cells in both lobes. In vitro taurocholate and bilirubin transport, as well as in vivo immunohistochemical Ntcp and Mrp2 expressions were bilaterally temporarily diminished, whereas LL and NLL structural acinar changes were divergent. In vivo bile and bilirubin-glucuronide excretion mirrored macroscopic changes, whereas serum bilirubin levels remained unaffected. In vivo functional imaging (indocyanine-green clearance test; (99mTc)-mebrofenin hepatobiliary scintigraphy; confocal laser endomicroscopy) indicated transitionally reduced global liver uptake and -excretion. While LL functional involution was permanent, NLL uptake and excretory functions recovered excessively. Following PVL, functioning cells remain even in LL. Despite extensive bilateral morpho-functional changes, NLL functional increment restores temporary declined transport functions, emphasising liver functional assessment
Relationship between alpha(s1)-casein variants on physicochemical properties of Sarda goat’s milk
Insulin-like growth factor-I-dependent stimulation of nuclear phospholipase C-beta1 activity in Swiss 3T3 cells requires an intact cytoskeleton and is paralleled by increased phosphorylation of the phospholipase.
7nonenoneMARTELLI AM; COCCO L; BAREGGI R; TABELLINI G; RIZZOLI R; GHIBELLINI MD; NARDUCCI PMartelli, Am; Cocco, L; Bareggi, R; Tabellini, Giovanna; Rizzoli, R; Ghibellini, Md; Narducci, P
Lineage-related susceptibility of human hemopoietic cell lines to apoptosis.
Apoptosis plays a fundamental role in shaping normal hematopoiesis. We have
investigated the relationship existing between susceptibility to apoptosis and
lineage commitment in hemopoietic cells. The presence and degree of apoptosis
were investigated in myeloid (HL-60 and K562), T (Jurkat and MOLT-4), and B (CESS
and Raji) lymphoid cell lines by using a variety of techniques-transmission
electron and light microscopy, flow cytometry and DNA gel electrophoresis. The
major achievement of this study is that hematopoietic cells respond to different
chemical (staurosporin, tiazofurin, camptothecin) and physical (hyperthermia or
hypothermia) stimuli by apoptosis in a lineage-related way. Moreover, with
respect to the methods used to detect apoptosis, a strong correlation was
observed between the presence of the hypodiploid peak determined by flow
cytometry and the DNA laddering evaluated by gel electrophoresis, but both
techniques failed to demonstrate the presence of apoptosis in some cases. We
conclude that cells of different hematopoietic lineages mostly show a
lineage-related behaviour in their apoptotic response to different stimuli,
suggesting that the lineage commitment and the stage of differentiation can
confer different sensitivities to specific apoptotic stimuli. Moreover,
morphological techniques still represent the most reliable approach to detect
apoptosis in hemopoietic cells