Transcriptional Regulation by CHIP/LDB Complexes

It is increasingly clear that transcription factors play versatile roles in turning genes “on” or “off” depending on cellular context via the various transcription complexes they form. This poses a major challenge in unraveling combinatorial transcription complex codes. Here we use the powerful genetics of Drosophila combined with microarray and bioinformatics analyses to tackle this challenge. The nuclear adaptor CHIP/LDB is a major developmental regulator capable of forming tissue-specific transcription complexes with various types of transcription factors and cofactors, making it a valuable model to study the intricacies of gene regulation. To date only few CHIP/LDB complexes target genes have been identified, and possible tissue-dependent crosstalk between these complexes has not been rigorously explored. SSDP proteins protect CHIP/LDB complexes from proteasome dependent degradation and are rate-limiting cofactors for these complexes. By using mutations in SSDP, we identified 189 down-stream targets of CHIP/LDB and show that these genes are enriched for the binding sites of APTEROUS (AP) and PANNIER (PNR), two well studied transcription factors associated with CHIP/LDB complexes. We performed extensive genetic screens and identified target genes that genetically interact with components of CHIP/LDB complexes in directing the development of the wings (28 genes) and thoracic bristles (23 genes). Moreover, by in vivo RNAi silencing we uncovered novel roles for two of the target genes, xbp1 and Gs-alpha, in early development of these structures. Taken together, our results suggest that loss of SSDP disrupts the normal balance between the CHIP-AP and the CHIP-PNR transcription complexes, resulting in down-regulation of CHIP-AP target genes and the concomitant up-regulation of CHIP-PNR target genes. Understanding the combinatorial nature of transcription complexes as presented here is crucial to the study of transcription regulation of gene batteries required for development

Real contact ratio and tooth bending stress calculation for plastic/plastic and plastic/steel spur gears

This paper presents an iterative method for calculating the effective contact ratio and the bending tooth stress for a pair of plastic/plastic and plastic/steel spur gears with an involute profile. In this method, the pinion and the gear are modeled, at each moment of the mesh cycle, as equivalent springs in parallel undergoing the same displacement along the line of action. This leads to the calculation of the bending stress by taking into account the number of teeth initially in contact and those which enter in contact prematurely. We also investigate the influence of certain gear parameters, such as, the number of teeth, the pressure angle, and the module on the behavior of a pair of meshed gears. In addition, the variation of the bending stress at the tooth fillet is investigated for a pair of plastic/plastic and a pair of plastic/steel spur gears, in order to determine the critical configurations for which the bending stress is maximum. In general, the results obtained from the present method also show that the stress variation in plastic/plastic gears differs markedly from that in plastic/steel gears