Case study: Optimization stage of a bispecific DART® molecule identified dissolved carbon dioxide (dCO2) is a critical upstream process parameter for growth, productivity and scalability

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Collective Hamiltonians with Kac-Moody Algebraic Conditions

We describe the general framework for constructing collective--theory Hamiltonians whose hermicity requirements imply a Kac--Moody algebra of constraints on the associated Jacobian. We give explicit examples for the algebras $sl(2)_k$ and $sl(3)_k$. The reduction to $W_n$--constraints, relevant to $n$-matrix models, is described for the Jacobians.Comment: Additional results and references; Phyzzte

The Role of Dynamin in Cell-Cell Fusion

Myoblast fusion leads to the formation of multinucleated muscle fibers and is essential for muscle development and regeneration. Drosophila embryonic muscle development has been an instrumental in vivo system to uncover evolutionarily conserved cellular and molecular mechanisms of myoblast fusion. Work from our lab has shown that myoblast fusion is promoted by a cell type-specific, F-actin-enriched podosome-like structure (PLS) that invades the apposing fusion partner with multiple finger-like protrusions at the fusogenic synapse. Here, we shown that the conserved large GTPase Dynamin (Dyn), best known for its function in endocytosis, is a critical component of myoblast fusion in vivo. Interference with Dyn function during myoblast fusion using two different temperature-sensitive alleles of Drosophila Dyn, shibire^ts (shi^ts), leads to a severe myoblast fusion defect, which can be rescued by overexpressing wild-type Dyn. Furthermore, RNAi knockdown of Dyn in cultured cells that are induced to fuse also results in a fusion defect, suggesting a general role for Dyn in cell-cell fusion. We show that Dyn is enriched within the F-actin foci at the fusogenic synapse in wild-type embryos and that the F-actin foci exhibit abnormal morphology in shi^ts mutant embryos at restrictive temperature, indicating a function of Dyn in organizing these actin-enriched structures. Interestingly, electron microscopy analysis revealed no endocytic vesicles at the fusogenic synapse where Dyn is enriched in wild-type embryos, and no collared pits indicative of blocked endocytosis are observed at the fusogenic synapse in shi^ts mutant embryos at restrictive temperature, suggesting that endocytosis may not play a direct role in myoblast fusion. Together, our findings strongly support a novel endocytosis-independent function of Dyn in regulating F-actin organization during cell-cell fusion

Search for Axionlike and Scalar Particles with the NA64 Experiment

We carried out a model-independent search for light scalar (s) and pseudoscalar axionlike (a) particles that couple to two photons by using the high-energy CERN SPS H4 electron beam. The new particles, if they exist, could be produced through the Primakoff effect in interactions of hard bremsstrahlung photons generated by 100 GeV electrons in the NA64 active dump with virtual photons provided by the nuclei of the dump. The a(s) would penetrate the downstream HCAL module, serving as shielding, and would be observed either through their $a(s)\to\gamma \gamma$ decay in the rest of the HCAL detector or as events with large missing energy if the a(s) decays downstream of the HCAL. This method allows for the probing the a(s) parameter space, including those from generic axion models, inaccessible to previous experiments. No evidence of such processes has been found from the analysis of the data corresponding to $2.84\times10^{11}$ electrons on target allowing to set new limits on the $a(s)\gamma\gamma$-coupling strength for a(s) masses below 55 MeV.Comment: This publication is dedicated to the memory of our colleague Danila Tlisov. 7 pages, 5 figures, revised version accepted for publication in Phys. Rev. Let

Measurement of the Omega_c Lifetime

We present the measurement of the lifetime of the Omega_c we have performed using three independent data samples from two different decay modes. Using a Sigma- beam of 340 GeV/c we have obtained clean signals for the Omega_c decaying into Xi- K- pi+ pi+ and Omega- pi+ pi- pi+, avoiding topological cuts normally used in charm analysis. The short but measurable lifetime of the Omega_c is demonstrated by a clear enhancement of the signals at short but finite decay lengths. Using a continuous maximum likelihood method we determined the lifetime to be tau(Omega_c) = 55 +13-11(stat) +18-23(syst) fs. This makes the Omega_c the shortest living weakly decaying particle observed so far. The short value of the lifetime confirms the predicted pattern of the charmed baryon lifetimes and demonstrates that the strong interaction plays a vital role in the lifetimes of charmed hadrons.Comment: 15 pages, including 7 figures; gzipped, uuencoded postscrip

Fast Photon Detection for Particle Identification with COMPASS RICH-1

Particle identification at high rates is an important challenge for many current and future high-energy physics experiments. The upgrade of the COMPASS RICH-1 detector requires a new technique for Cherenkov photon detection at count rates of several $10^6$ per channel in the central detector region, and a read-out system allowing for trigger rates of up to 100 kHz. To cope with these requirements, the photon detectors in the central region have been replaced with the detection system described in this paper. In the peripheral regions, the existing multi-wire proportional chambers with CsI photocathode are now read out via a new system employing APV pre-amplifiers and flash ADC chips. The new detection system consists of multi-anode photomultiplier tubes (MAPMT) and fast read-out electronics based on the MAD4 discriminator and the F1-TDC chip. The RICH-1 is in operation in its upgraded version for the 2006 CERN SPS run. We present the photon detection design, constructive aspects and the first Cherenkov light in the detector.Comment: Proceedings of the Imaging 2006 conference, Stockholm, Sweden, 27-30 June 2006, 5 pages, 6 figures, to appear in NIM A; corrected typo in caption of Fig.

Fast photon detection for the COMPASS RICH detector

The COMPASS experiment at the SPS accelerator at CERN uses a large scale Ring Imaging CHerenkov detector (RICH) to identify pions, kaons and protons in a wide momentum range. For the data taking in 2006, the COMPASS RICH has been upgraded in the central photon detection area (25% of the surface) with a new technology to detect Cherenkov photons at very high count rates of several 10^6 per second and channel and a new dead-time free read-out system, which allows trigger rates up to 100 kHz. The Cherenkov photons are detected by an array of 576 visible and ultra-violet sensitive multi-anode photomultipliers with 16 channels each. The upgraded detector showed an excellent performance during the 2006 data taking.Comment: Proceeding of the IPRD06 conference (Siena, Okt. 06

Search for the exotic Ξ−−(1860)\Xi^{--}(1860) Resonance in 340GeV/c Σ−\Sigma^--Nucleus Interactions

We report on a high statistics search for the $\Xi^{--}(1860)$ resonance in $\Sigma^-$-nucleus collisions at 340GeV/c. No evidence for this resonance is found in our data sample which contains 676000 $\Xi^-$ candidates above background. For the decay channel $\Xi^{--}(1860) \to \Xi^-\pi^-$ and the kinematic range 0.15$<x_F<$0.9 we find a 3$\sigma$ upper limit for the production cross section of 3.1 and 3.5 $\mu$b per nucleon for reactions with carbon and copper, respectively.Comment: 5 pages, 4 figures, modification of ref. 43 and 4