Lentil-based snack: development and nutritious properties

Legume grains provide not only a unique nutrient profile essential for good human health, such as protein, fiber, carbohydrates, fatty acids, minerals, vitamins, and phenolic compounds, but they also provide excellent environmental benefits. Besides, they adapt to contemporary food patterns, like flexitarian, vegetarian, vegan, and gluten-free. However, legume consumption continues to be less than desired, opening new opportunities for different intake alternatives. Furthermore, due to agriculture growth and unbalanced animal consumption the food industry aims to increase and diversify alternative protein formulations. Amongst the legumes, lentils have a fast-cooking time, high arginine, antioxidant flavonoids, and a low glycemic index which helps avoid peaks in blood glucose, improving metabolic control, and recent epidemiological studies suggest that they may help combat cardiovascular disease and diabetes mellitus, both with high prevalence worldwide. Based on this knowledge, we developed a muffin in which commercial oatmeal was partially substituted with lentil flour. The nutritional value of the snack was evaluated, and it was also performed microbiological analyses. The lentil-based muffin had higher protein and fiber and lower carbohydrate content compared to the oatmeal-based muffin. The aim of these muffins is that they are suitable for all consumers, including diabetics. This project seeks to be a central element in the promotion of one of the oldest grain legumes, contributing to an increase in its consumption and consequent commercial valorization.info:eu-repo/semantics/publishedVersio

Labrys portucalensis F11 efficiently degrades Di-(2-ethylhexyl) Phthalate

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Lentil (lens culinaris L.) intraspecific nutritional variability and development of a lentil-based snack

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Development of a healthy lentil-based food product

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Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

The shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiver sity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxo nomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world’s known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world’s most biodiverse countries. We further identify collection gaps and summarize future goals that extend be yond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still un equally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the coun try. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora.Fil: Gomes da Silva, Janaina. Jardim Botânico do Rio de Janeiro: Rio de Janeiro, BrasilFil: Filardi, Fabiana L.R. Jardim Botânico do Rio de Janeiro; BrasilFil: Barbosa, María Regina de V. Universidade Federal da Paraíba: Joao Pessoa; BrasilFil: Baumgratz, José Fernando Andrade. Jardim Botânico do Rio de Janeiro; BrasilFil: de Mattos Bicudo, Carlos Eduardo. Instituto de Botânica. Núcleo de Pesquisa em Ecologia; BrasilFil: Cavalcanti, Taciana. Empresa Brasileira de Pesquisa Agropecuária Recursos Genéticos e Biotecnologia; BrasilFil: Coelho, Marcus. Prefeitura Municipal de Campinas; BrasilFil: Ferreira da Costa, Andrea. Federal University of Rio de Janeiro. Museu Nacional. Department of Botany; BrasilFil: Costa, Denise. Instituto de Pesquisas Jardim Botanico do Rio de Janeiro; BrasilFil: Dalcin, Eduardo C. Rio de Janeiro Botanical Garden Research Institute; BrasilFil: Labiak, Paulo. Universidade Federal do Parana; BrasilFil: Cavalcante de Lima, Haroldo. Jardim Botânico do Rio de Janeiro; BrasilFil: Lohmann, Lucia. Universidade de São Paulo; BrasilFil: Maia, Leonor. Universidade Federal de Pernambuco; BrasilFil: Mansano, Vidal de Freitas. Instituto de Pesquisas Jardim Botânico do Rio de Janeiro; Brasil. Jardim Botânico do Rio de Janeiro; BrasilFil: Menezes, Mariângela. Federal University of Rio de Janeiro. Museu Nacional. Department of Botany; BrasilFil: Morim, Marli. Instituto de Pesquisas Jardim Botânico do Rio de Janeiro; BrasilFil: Moura, Carlos Wallace do Nascimento. Universidade Estadual de Feira de Santana. Department of Biological Science; BrasilFil: Lughadha, Eimear NIck. Royal Botanic Gardens; Reino UnidoFil: Peralta, Denilson. Instituto de Pesquisas Ambientais; BrazilFil: Prado, Jefferson. Instituto de Pesquisas Ambientais; BrasilFil: Roque, Nádia. Universidade Federal da Bahia; BrasilFil: Stehmann, Joao. Universidade Federal de Minas Gerais; BrasilFil: da Silva Sylvestre, Lana. Universidade Federal do Rio de Janeiro; BrasilFil: Trierveiler-Pereira, Larissa. Universidade Estadual de Maringá. Departamento de Análises Clínicas e Biomedicina; BrasilFil: Walter, Bruno Machado Teles. EMBRAPA Cenargen Brasília; BrasilFil: Zimbrão, Geraldo. Universidade Federal do Rio de Janeiro; BrasilFil: Forzza, Rafaela C. Jardim Botânico do Rio de Janeiro; BrasilFil: Morales, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Agronomía y Ciencias Agroalimentarias; Argentin

Anti-malarial activity and toxicity assessment of Himatanthus articulatus, a plant used to treat malaria in the Brazilian Amazon

Background: Plasmodium falciparum has become resistant to some of the available drugs. Several plant species are used for the treatment of malaria, such as Himatanthus articulatus in parts of Brazil. The present paper reports the phyto-chemistry, the anti-plasmodial and anti-malarial activity, as well as the toxicity of H. articulatus. Methods: Ethanol and dichloromethane extracts were obtained from the powder of stem barks of H. articulates and later fractionated and analysed. The anti-plasmodial activity was assessed against a chloroquine resistant strain P. falciparum (W2) in vitro, whilst in vivo anti-malarial activity against Plasmodium berghei (ANKA strain) was tested in mice, evaluating the role of oxidative stress (total antioxidant capacity - TEAC; lipid peroxidation – TBARS, and nitrites and nitrates - NN). In addition, cytotoxicity was evaluated using the HepG2 A16 cell-line. The acute oral and sub-chronic toxicity of the ethanol extract were evaluated in both male and female mice. Results: Plumieride was isolated from the ethyl acetate fraction of ethanol extract, Only the dichloromethane extract was active against clone W2. Nevertheless, both extracts reduced parasitaemia in P. berghei-infected mice. Besides, a significant reduction in pulmonary and cerebral levels of NN (nitrites and nitrates) was found, as well as in pulmonary TBARS, indicating a reduced oxidative damage to these organs. The ethanol extract showed low cytotoxicity to HepG2 A16 cells in the concentrations used. No significant changes were observed in the in vivo toxicity studies. Conclusions: The ethanol extract of H. articulatus proved to be promising as anti-malarial medicine and showed low toxicity

Aire Disruption Influences the Medullary Thymic Epithelial Cell Transcriptome and Interaction With Thymocytes

The function of medullary thymic epithelial cells (mTECs) is associated with thymocyte adhesion, which is crucial for the negative selection of autoreactive thymocytes in the thymus. This process represents the root of central tolerance of self-components and prevents the onset of autoimmune diseases. Since thymic epithelia correspond to an important target of donor T cells during the onset of chronic graft-vs-host-disease, mTEC-thymocyte adhesion may have implications for alloimmunity. The Aire and Fezf2 genes function as transcriptome controllers in mTECs. The central question of this study is whether there is a mutual relationship between mTEC-thymocyte adhesion and the control of the mTEC transcriptome and whether Aire is involved in this process. Here, we show that in vitro mTEC-thymocyte adhesion causes transcriptome changes in mTECs and upregulates the transcriptional expression of Aire and Fezf2, as well as cell adhesion-related genes such as Cd80 or Tcf7, among others. Crispr-Cas9-mediated Aire gene disruption demonstrated that this gene plays a role in the process of mTEC-thymocyte adhesion. Consistent with the nuclear localization signal (NLS) encoded by Aire exon 3, which was targeted, we demonstrate that Aire KO−/− mTECs impair AIRE protein localization in the nucleus. Consequently, the loss of function of Aire reduced the ability of these cells to adhere to thymocytes. Their transcriptomes differed from their wild-type Aire+/+ counterparts, even during thymocyte adhesion. A set of mRNA isoforms that encode proteins involved in cell adhesion were also modulated during this process. This demonstrates that both thymocyte interactions and Aire influence transcriptome profiling of mTEC cells

Lentil (Lens culinaris L.) intraspecific nutritional variability and development of a lentil-based snack

info:eu-repo/semantics/publishedVersio

Image_4_Aire Disruption Influences the Medullary Thymic Epithelial Cell Transcriptome and Interaction With Thymocytes.TIF

<p>The function of medullary thymic epithelial cells (mTECs) is associated with thymocyte adhesion, which is crucial for the negative selection of autoreactive thymocytes in the thymus. This process represents the root of central tolerance of self-components and prevents the onset of autoimmune diseases. Since thymic epithelia correspond to an important target of donor T cells during the onset of chronic graft-vs-host-disease, mTEC-thymocyte adhesion may have implications for alloimmunity. The Aire and Fezf2 genes function as transcriptome controllers in mTECs. The central question of this study is whether there is a mutual relationship between mTEC-thymocyte adhesion and the control of the mTEC transcriptome and whether Aire is involved in this process. Here, we show that in vitro mTEC-thymocyte adhesion causes transcriptome changes in mTECs and upregulates the transcriptional expression of Aire and Fezf2, as well as cell adhesion-related genes such as Cd80 or Tcf7, among others. Crispr-Cas9-mediated Aire gene disruption demonstrated that this gene plays a role in the process of mTEC-thymocyte adhesion. Consistent with the nuclear localization signal (NLS) encoded by Aire exon 3, which was targeted, we demonstrate that Aire KO^−/− mTECs impair AIRE protein localization in the nucleus. Consequently, the loss of function of Aire reduced the ability of these cells to adhere to thymocytes. Their transcriptomes differed from their wild-type Aire^+/+ counterparts, even during thymocyte adhesion. A set of mRNA isoforms that encode proteins involved in cell adhesion were also modulated during this process. This demonstrates that both thymocyte interactions and Aire influence transcriptome profiling of mTEC cells.</p

Image_3_Aire Disruption Influences the Medullary Thymic Epithelial Cell Transcriptome and Interaction With Thymocytes.TIF

<p>The function of medullary thymic epithelial cells (mTECs) is associated with thymocyte adhesion, which is crucial for the negative selection of autoreactive thymocytes in the thymus. This process represents the root of central tolerance of self-components and prevents the onset of autoimmune diseases. Since thymic epithelia correspond to an important target of donor T cells during the onset of chronic graft-vs-host-disease, mTEC-thymocyte adhesion may have implications for alloimmunity. The Aire and Fezf2 genes function as transcriptome controllers in mTECs. The central question of this study is whether there is a mutual relationship between mTEC-thymocyte adhesion and the control of the mTEC transcriptome and whether Aire is involved in this process. Here, we show that in vitro mTEC-thymocyte adhesion causes transcriptome changes in mTECs and upregulates the transcriptional expression of Aire and Fezf2, as well as cell adhesion-related genes such as Cd80 or Tcf7, among others. Crispr-Cas9-mediated Aire gene disruption demonstrated that this gene plays a role in the process of mTEC-thymocyte adhesion. Consistent with the nuclear localization signal (NLS) encoded by Aire exon 3, which was targeted, we demonstrate that Aire KO^−/− mTECs impair AIRE protein localization in the nucleus. Consequently, the loss of function of Aire reduced the ability of these cells to adhere to thymocytes. Their transcriptomes differed from their wild-type Aire^+/+ counterparts, even during thymocyte adhesion. A set of mRNA isoforms that encode proteins involved in cell adhesion were also modulated during this process. This demonstrates that both thymocyte interactions and Aire influence transcriptome profiling of mTEC cells.</p