Attribute and technology value mapping for conceptual product design phase

This is a pre-copyedited, author-produced PDF of an article accepted for publication in Journal of Mechanical Engineering Science following peer review. The final, definite version of this paper has been published in Journal of Mechanical Engineering Science, Aris Georgiou, George Haritos, Moyra Fowler, and Yasmin Imani, ‘Attribute and technology value mapping for conceptual product design phase’, Vol. 230(11): 1745-1756, May 2016, published by SAGE Publishing, available online at doi: https://doi.org/10.1177/0954406215585595. Copyright © 2016 The Author(s).The main focus of this paper is how the concept design phase of the product development process can be improved by using an objective data-driven approach in selecting a final concept design to progress further. A quantitative new test-bed ‘Product Optimisation Value Engineering’ (PROVEN) is presented to critically assess new and evolving powertrain technologies at the concept design phase. The new test-bed has the ability to define a technology value map to assess multiple technical options as a function of its attributes, whose precise values can be determined at a given cost. A mathematical model that incorporates a highly adaptable, data-driven and multi-attribute value approach to product specification and conceptual design is developed, novel to the concept design process. This creates a substantially optimised product offering to the market, reducing overall development costs while achieving customer satisfactionPeer reviewe

Toward a Theory of Innovation

The purpose of this article is to eliminate further conceptual obstacles to the develop ment of a workable theory of innovation and to move toward a better theoretic statement. The approach to overcoming the conceptual problems centers primarily around four ideas: (1) building a theory around the "innovation decision" as the unit of analysis, rather than either innovations or adopters: (2) lifting the level of general ity of independent variables so that a great deal of statistical interaction is avoided; (3) splitting the act of innovation into two stages, diffusion and adoption, to eliminate the confounding effects of time of awareness in studies of innovation; (4) introducing the idea of a "fair-trial point" into the conceptualization of innovation, solving sev eral additional problems at once.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68286/2/10.1177_009539977901000401.pd

The N-terminal intrinsically disordered domain of mgm101p is localized to the mitochondrial nucleoid.

The mitochondrial genome maintenance gene, MGM101, is essential for yeasts that depend on mitochondrial DNA replication. Previously, in Saccharomyces cerevisiae, it has been found that the carboxy-terminal two-thirds of Mgm101p has a functional core. Furthermore, there is a high level of amino acid sequence conservation in this region from widely diverse species. By contrast, the amino-terminal region, that is also essential for function, does not have recognizable conservation. Using a bioinformatic approach we find that the functional core from yeast and a corresponding region of Mgm101p from the coral Acropora millepora have an ordered structure, while the N-terminal domains of sequences from yeast and coral are predicted to be disordered. To examine whether ordered and disordered domains of Mgm101p have specific or general functions we made chimeric proteins from yeast and coral by swapping the two regions. We find, by an in vivo assay in S.cerevisiae, that the ordered domain of A.millepora can functionally replace the yeast core region but the disordered domain of the coral protein cannot substitute for its yeast counterpart. Mgm101p is found in the mitochondrial nucleoid along with enzymes and proteins involved in mtDNA replication. By attaching green fluorescent protein to the N-terminal disordered domain of yeast Mgm101p we find that GFP is still directed to the mitochondrial nucleoid where full-length Mgm101p-GFP is targeted

Highly ionized Fe K emission lines from the LINER galaxy M 81

We present spectral and timing results from a long (130 ks) XMM-NEWTON EPIC observation of the nucleus of the Seyfert/LINER galaxy M 81. During the observation the X-ray flux varied by 20%, but there was no significant change in spectral shape. The 2-10 keV spectrum is well described by a power law continuum and three narrow Fe K emission lines at 6.4, 6.7 and 6.96 keV. The three emission lines have equivalent widths of 39, 47, and 37 eV respectively. The ratios of the three lines are thus more similar to those observed from the Galactic Centre region than to those typically observed from Seyfert galaxies. The high ionization lines most likely originate either from photoionized gas within 0.1 pc of the nucleus of M 81, or from a non-thermal distribution of cosmic-ray electrons interacting with the 0.2-0.6 keV thermal plasma which is found in the bulge of M 81.Comment: Accepted for publication in A&

Biochemical and molecular studies of 132 patients with galactosemia

We evaluated 132 galactosemia patients for the Q188R (glutamine-188 to arginine) mutation in the human galactose-1-phosphate uridyltransferase (GALT) gene and for GALT activity in their hemolysates by a sensitive radioisotopic method. In those without any detectable GALT activity (GG), the Q188R mutation constituted 67% of the alleles. In patients with detectable GALT activity (GV), only 16% of the alleles were accounted for by Q188R. In all patients who were homozygous for the Q188R mutation, no erythrocyte GALT activity could be demonstrated. There was an extensive variation in the amount of detectable GALT activity ranging from 0.1% to 5% of the normal values among the GV patients. There was a difference in the frequency of Q188R mutation in the GALT alleles among patients belonging to different racial and ethnic groups. In Caucasian and Hispanic patients, the frequency was not far different (64% and 58%, respectively). On the other hand, only 12% of the GALT alleles with Q188R were found in African-American patients.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47637/1/439_2004_Article_BF00201593.pd

The Current State of Performance Appraisal Research and Practice: Concerns, Directions, and Implications

On the surface, it is not readily apparent how some performance appraisal research issues inform performance appraisal practice. Because performance appraisal is an applied topic, it is useful to periodically consider the current state of performance research and its relation to performance appraisal practice. This review examines the performance appraisal literature published in both academic and practitioner outlets between 1985 and 1990, briefly discusses the current state of performance appraisal practice, highlights the juxtaposition of research and practice, and suggests directions for further research

Pregnancy postponement and childlessness leads to chronic hypervascularity of the breasts and cancer risk

Epidemiologists have established that women with small families, and particularly nulliparae, are prone to develop breast cancer later in life. We report that physiological mammary hypervascularity may be an intermediate reason against the background that breast-core vascularity is normal in pregnancy but pathological in the vascularisation of cancer. We examined breast ‘core’ vascularity in nulliparae during their potential reproductive life and in parous women after their last birth but before their menopause. Fifty clinically normal pre-menopausal non-pregnant women (100 breasts) were studied daily for one ‘luteal positive’ menstrual cycle. Their parity history varied from zero to five babies. Under controlled domestic conditions each wore a special electronic thermometric bra to automatically record breast ‘core’ temperature changes as a measure of mammary tissue blood flow. In the nulliparae there was a rise of breast vascularity throughout reproductive life. In the parous women, a year or so after each birth, breast vascularity was reset at a lower level than before the pregnancy; thereafter, as in nulliparae, there was progressive increase in mammary vascularity until the menopause

A Defined, Feeder-Free, Serum-Free System to Generate In Vitro Hematopoietic Progenitors and Differentiated Blood Cells from hESCs and hiPSCs

Human ESC and iPSC are an attractive source of cells of high quantity and purity to be used to elucidate early human development processes, for drug discovery, and in clinical cell therapy applications. To efficiently differentiate pluripotent cells into a pure population of hematopoietic progenitors we have developed a new 2-dimentional, defined and highly efficient protocol that avoids the use of feeder cells, serum or embryoid body formation. Here we showed that a single matrix protein in combination with growth factors and a hypoxic environment is sufficient to generate from pluripotent cells hematopoietic progenitors capable of differentiating further in mature cell types of different lineages of the blood system. We tested the differentiation method using hESCs and 9 iPSC lines generated from different tissues. These data indicate the robustness of the protocol providing a valuable tool for the generation of clinical-grade hematopoietic cells from pluripotent cells

Centre of pressure characteristics in normal, planus and cavus feet

Background The aim of this study was to compare centre of pressure (COP) characteristics between healthy adults with normal, planus or cavus feet who were allocated to groups based on reliable foot posture measurement techniques. Methods Ninety-two healthy adult participants (aged 18 to 45) were recruited and classified as either normal (n = 35), pes planus (n = 31) or pes cavus (n = 26) based on Foot Posture Index, Arch Index and normalised navicular height truncated measurements. Barefoot walking trials were conducted using an emed®-x 400 plantar pressure system (Novel GmbH, Munich, Germany). Average, maximum, minimum and range (difference between maximum and minimum) values were calculated for COP velocity and lateral-medial force index during loading response, midstance, terminal stance and pre-swing phases of stance. The COP excursion index was also calculated. One-way analyses of variance were used to compare the three foot posture groups. Results The cavus foot exhibited the slowest average and minimum COP velocity during terminal stance, but this pattern was reversed during pre-swing, when the cavus foot exhibited the fastest maximum COP velocity. The planus foot exhibited the smallest lateral medial force index range during terminal stance. There were no differences between the groups for COP excursion index. Conclusion These findings indicate that there are differences in COP characteristics between foot postures, which may represent different mechanisms for generating force to facilitate forward progression of the body during the propulsive phases of gait