66 research outputs found
Relational Lockdown and Relational Trauma in the Time of Coronavirus: A Reflection from a UK Family Therapist
This is the final version. Available on open access from Wiley via the DOI in this recordLike a meteor hitting the earthâs surface, 41,698* unexpected deaths have shaken, disturbed, and saddened the core of our nation. This reflection considers the consequences of the coronavirus crisis in the UK with particular reference to the impact on families and on the practice of family therapists. The perspective presented can only be partial because of the fastâchanging situation and the limited access to alternative perspectives that are available during this period of relational lockdown. The author provides a systemic understanding of what has happened and what is happening
Profiling Insulin Like Factor 3 (INSL3) Signaling in Human Osteoblasts
Abstract
BACKGROUND:
Young men with mutations in the gene for the INSL3 receptor (Relaxin family peptide 2, RXFP2) are at risk of reduced bone mass and osteoporosis. Consistent with the human phenotype, bone analyses of Rxfp2(-/-) mice showed decreased bone volume, alterations of the trabecular bone, reduced mineralizing surface, bone formation, and osteoclast surface. The aim of this study was to elucidate the INSL3/RXFP2 signaling pathways and targets in human osteoblasts.
METHODOLOGY/PRINCIPAL FINDINGS:
Alkaline phosphatase (ALP) production, protein phosphorylation, intracellular calcium, gene expression, and mineralization studies have been performed. INSL3 induced a significant increase in ALP production, and Western blot and ELISA analyses of multiple intracellular signaling pathway molecules and their phosphorylation status revealed that the MAPK was the major pathway influenced by INSL3, whereas it does not modify intracellular calcium concentration. Quantitative Real Time PCR and Western blotting showed that INSL3 regulates the expression of different osteoblast markers. Alizarin red-S staining confirmed that INSL3-stimulated osteoblasts are fully differentiated and able to mineralize the extracellular matrix.
CONCLUSIONS/SIGNIFICANCE:
Together with previous findings, this study demonstrates that the INSL3/RXFP2 system is involved in bone metabolism by acting on the MAPK cascade and stimulating transcription of important genes of osteoblast maturation/differentiation and osteoclastogenesis
Healing through culturally embedded practice: an investigation of counsellorsâ and clientsâ experiences of Buddhist Counselling in Thailand
This thesis is concerned with an exploration of counsellorsâ and clientsâ lived experiences of
Buddhist Counselling, an indigenous Buddhist-based counselling approach in Thailand. Over
the past decade, Buddhist Counselling has received a growing interest from Thai counselling
trainees and practitioners, and it has also expanded to serve Thai people in various settings.
Research on Buddhist Counselling is very limited and most of the existing studies in the
field have focused on measuring the effectiveness of the approach. While these studies have
consistently indicated the positive effects of Buddhist Counselling on psychological
improvement across several population groups, the significant questions of how Buddhist
Counselling brings about such outcome and how it is experienced are still largely
unanswered. Moreover, existing research is concentrated much more on clientsâ views than
counsellorsâ views, although counsellorsâ views of their counselling practice can also serve as
a knowledge base of the field. This thesis thus sets out to contribute to rectifying this
omission by exploring Buddhist Counselling from the perspectives of both counsellors and
clients.
The thesis is based on two qualitative studies. The first study addressed Buddhist
Counselling from the perspective of five counsellors through a focus group and semi-structured
interviews. The second study explored Buddhist Counselling from the perspective
of three clients, using two semi-structured interviews with each of them. All data received
were analysed using interpretative phenomenological analysis (IPA).
The study reveals counsellorsâ and clientsâ overall positive experience of engaging in
Buddhist Counselling. Central to the accounts of the counsellors are the following
perceptions: that their practice of Buddhist Counselling is culturally congruent with the
existing values and beliefs of both themselves and their clients; that their personal and
professional congruence is key to their therapeutic efficacy; and that they enhance such
congruence through their application of Buddhist ideas and practices in their daily lives. Key
to the clientsâ accounts is their emphasis on the significant roles of the counsellorsâ Buddhist
ideas and personal qualities, and of their religious practices in facilitating healing and change.
Key shared findings from both studies reveal that the participantsâ accounts of their cultural
background and their experiences of Buddhist Counselling are intertwined. Adopting
hermeneutics to address this intertwinement, I reveal the cultural and moral dimensions
underlying the practice of Buddhist Counselling. Based on such revelation, I suggest that
Buddhist Counselling in particular, as well as psychotherapy in general, should be better
understood as a historically situated, culturally bound, and morally constituted activity of
people who are concerned with improving the quality of their lives and their community,
rather than the transcultural and merely relational work of morally-neutral practitioners
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