Elemental composition of sole otoliths as a population discrimination tool

Otolith microchemistry, the study of the minor and trace elemental composition of otoliths (earbones), has been developing rapidly with a wide range of applications in fishery science. This is because otoliths (1) grow continuously, resulting in daily and seasonal structures, (2) have an elemental composition that reflects the environmental concentrations and conditions, (3) are metabolically inert. All of these characteristics make trace element uptake useful for reconstructing environmental histories. The elemental composition is therefore a powerful tool in stock To study connectivity among North Sea sole (Solea solea) populations and to improve our understanding of the relationship between its spawning grounds and nursery areas, genetic markers and otolith microchemistry will be used as complementary discrimination tools. We performed a pilot study to test for differences in sole otolith elemental fingerprints among three sampling locations, using LA-ICPMS. This technique makes it possible to determine composition at discrete points across the otolith (corresponding with different events in the fish’s lifetime). A hierarchical design (Basin, Sea, within Sea) enables us to assess the power of this method for the simultaneous large and small scale discrimination of populations.Preliminary results of this study will be shown and discussed in the light of current European wide research objectives. size and increasing the number of sampling locations

Experimental study of the e+e- -> pi0 gamma process in the energy region sqrt(s)=0.60-0.97 GeV

Results of the study of the e+e-->pi0 gamma process with SND detector at VEPP-2M collider in the c.m.s. energy range sqrt(s)=0.60-0.97 GeV are presented. Using 36513 selected events corresponding to a total integrated luminosity of 3.4 pb^-1 the e+e-->pi0 gamma cross section was measured. The energy dependence of the cross section was analyzed in the framework of the vector meson dominance model. The data are well described by a sum of phi,omega,rho0->pi0 gamma decay contributions with measured decay probabilities: Br(omega->pi0 gamma)=(9.34+-0.15+-0.31)% and Br(rho0->pi0 gamma)=(5.15+-1.16+-0.73)*10^-4 . The rho-omega relative interference phase is phi(rho,omega}=(-10.2+-6.5+-2.5) degree

New measurement of the rare decay ϕ→η′γ\phi \to \eta' \gamma with CMD-2

A new measurement of the rare decay \phi \to \eta' \gamma performed with the CMD-2 detector at Novosibirsk is described. Of the data sample corresponding to the integrated luminosity of 14.5 pb^{-1}, twenty one events have been selected in the mode \eta'\to\pi^+\pi^-\eta, \eta\to\gamma\gamma. The following branching ratio was obtained: B(\phi \to \eta' \gamma) = (8.2^{+2.1}_{-1.9} \pm 1.1) 10^{-5}.Comment: 12 pages, 4 figures, LaTe

Proteogenomic landscape of breast cancer tumorigenesis and targeted therapy

The integration of mass spectrometry-based proteomics with next-generation DNA and RNA sequencing profiles tumors more comprehensively. Here this "proteogenomics" approach was applied to 122 treatment-naive primary breast cancers accrued to preserve post-translational modifications, including protein phosphorylation and acetylation. Proteogenomics challenged standard breast cancer diagnoses, provided detailed analysis of the ERBB2 amplicon, defined tumor subsets that could benefit from immune checkpoint therapy, and allowed more accurate assessment of Rb status for prediction of CDK4/6 inhibitor responsiveness. Phosphoproteomics profiles uncovered novel associations between tumor suppressor loss and targetable kinases. Acetylproteome analysis highlighted acetylation on key nuclear proteins involved in the DNA damage response and revealed cross-talk between cytoplasmic and mitochondrial acetylation and metabolism. Our results underscore the potential of proteogenomics for clinical investigation of breast cancer through more accurate annotation of targetable pathways and biological features of this remarkably heterogeneous malignancy