Evaluasi Penyimpanan Dan Pendistribusian Vaksin Dari Dinas Kesehatan Kota Manado Ke Puskesmas Tuminting, Puskesmas Paniki Bawah Dan Puskesmas Wenang

EVALUASI PENYIMPANAN DAN PENDISTRIBUSIAN VAKSIN DARI DINAS KESEHATAN KOTA MANADO KE PUSKESMAS TUMINTING, PUSKESMAS PANIKI BAWAH DAN PUSKESMAS WENANG Gebbie Prisiliya Lumentut1) , Nancy C. Pelealu1) , A. C. Wullur2) 1) Program Studi Farmasi FMIPA UNSRAT Manado, 95115 2) POLTEKES Manado, 95115 ABSTRACT The vaccine was a biological product which made from bacteria and have been weaekened, shut useful to stimulate immunity. The study states 75% of the vaccine in Indonesia exposed to freezing temperatures during distribution so that the vaccine cannot be used anymore. Health Departement was responsible for the implementation of the storage and distribution of vaccines on a regular and timely to the basic health care units. This study aimed to evaluate the storage and distribution of vaccines from Manado City Health Departement to the District Health Center of Tuminting, Paniki Bawah and Wenang using the descriptive observational methods and evaluation of the data collected prospectively. The results showed that the Health Departement of Manado City, Tuminting health center, Paniki Bawah health center and Wenang health center not in accordance with the guidenlines in the management of cold chain storage and distribution of vaccines that can be seen from the absence of temperature gauges, freeze tag, does not have a generator, do not have indicators freezers and cold boxes lack of liquid in a cool box for distribution. Keywords: Storage, Distribution, Vaccines, District Health Center ABSTRAK Vaksin adalah suatu produk biologi yang terbuat dari kuman dan telah dilemahkan, dimatikan berguna untuk merangsang kekebalan tubuh. Studi menyatakan 75% vaksin di Indonesia terpapar suhu beku selama pendistribusian sehingga vaksin tidak bisa digunakan lagi. Dinas Kesehatan bertanggung jawab terhadap terlaksananya penyimpanan dan pendistribusian vaksin yang teratur dan tepat waktu sampai kepada unit pelayanan kesehatan dasar. Penelitian ini bertujuan untuk mengevalusi penyimpanan dan pendistribusian vaksin dari Dinas Kesehatan Kota Manado ke Puskesmas Tuminting, Puskesmas Paniki Bawah dan Puskesmas Wenang dengan menggunakan metode observasional yang bersifat deskriptif dan evaluasi dengan teknik pengumpulan data secara prospektif. Hasil penelitian menunjukkan bahwa Dinas Kesehatan Kota Manado, Puskesmas Tuminting, Puskesmas Paniki Bawah dan Puskesmas Wenang belum sesuai dengan pedoman pengelolaan cold chain dalam hal penyimpanan dan pendistribusian vaksin yang terlihat dari tidak adanya alat pengukur suhu, freeze tag, tidak memiliki genset, tidak memiliki indikator pembeku dan terbatasnya kotak dingin cair dalam kotak dingin selama pendistribusian

General relativistic analysis of peculiar velocities

We give a careful general relativistic and (1+3)-covariant analysis of cosmological peculiar velocities induced by matter density perturbations in the presence of a cosmological constant. In our quasi-Newtonian approach, constraint equations arise to maintain zero shear of the non-comoving fundamental worldlines which define a Newtonian-like frame, and these lead to the (1+3)-covariant dynamical equations, including a generalized Poisson-type equation. We investigate the relation between peculiar velocity and peculiar acceleration, finding the conditions under which they are aligned. In this case we find (1+3)-covariant relativistic generalizations of well-known Newtonian results.Comment: 8 pages, LaTeX2e (iopart); minor changes, matches version accepted for publication by Classical and Quantum Gravit

Benthic foraminiferal stable carbon isotope constraints on deglacial ocean circulation and carbon-cycle changes

How does deep-ocean circulation influence atmospheric CO2 across deglacial transitions? Although biogeochemical and physical processes complicate interpretation of foraminiferal stable carbon isotope data, these complications can be addressed with expanded data compilations, multiproxy approaches, and model-data assimilation efforts.Fil: Peterson, Carlye D.. University of California Riverside; Estados UnidosFil: Gebbie, G.. Woods Hole Oceanographic Institution; Estados UnidosFil: Lisiecki, L. E.. University of California Santa Barbara; Estados UnidosFil: Lynch Stieglitz, J.. School of Earth and Atmospheric Sciences; Estados UnidosFil: Oppo, D.. Woods Hole Oceanographic Institution; Estados UnidosFil: Muglia, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; ArgentinaFil: Repschläger, Janne. Max Planck Institute for Chemistry; AlemaniaFil: Schmittner, A.. University of Oregon; Estados Unido

The rotation spectrum of methyl alcohol from 20 cm-1 to 80 cm-1

The rotational spectrum of methanol vapor has been measured from 20 cm-1 to 80 cm-1. The observations have been compared with the predictions of Burkhard and Dennison and for the most part good agreement is found. Some differences are found and since the new observations offer more reliable data with which to determine the energy levels a re-examination of the problem has been made.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32262/1/0000324.pd

A cyclic bipolar wind in the interacting binary V393 Scorpii

V393 Scorpii is a Double Periodic Variable characterized by a relatively stable non-orbital photometric cycle of 253 days. Mennickent et al. argue for the presence of a massive optically thick disc around the more massive B-type component and describe the evolutionary stage of the system. In this paper we analyze the behavior of the main spectroscopic optical lines during the long non-orbital photometric cycle. We study the radial velocity of the donor determining their orbital elements and find a small but significant orbital eccentricity (e = 0.04). The donor spectral features are modeled and removed from the spectrum at every observing epoch using the light-curve model given by Mennickent et al. We find that the line emission is larger during eclipses and mostly comes from a bipolar wind. We find that the long cycle is explained in terms of a modulation of the wind strength; the wind has a larger line and continuum emissivity on the high state. We report the discovery of highly variable chromospheric emission in the donor, as revealed by Doppler maps of the emission lines MgII 4481 and CI 6588. We discuss notable and some novel spectroscopic features like discrete absorption components, especially visible at blue-depressed OI 7773 absorption wings during the second half-cycle, Balmer double emission with V/R-curves showing "Z-type" and "S-type" excursions around secondary and main eclipse, respectively, and H_beta emission wings extending up to +- 2000 km/s. We discuss possible causes for these phenomena and for their modulations with the long cycle.Comment: 19 pages, 22 figures, accepted for publication in MNRA

Atlantic circulation change still uncertain

Deep oceanic overturning circulation in the Atlantic (Atlantic Meridional Overturning Circulation, AMOC) is projected to decrease in the future in response to anthropogenic warming. Caesar et al. 1 argue that an AMOC slowdown started in the 19 th century and intensified during the mid-20th century. Although the argument and selected evidence proposed have some merits, we find that their conclusions might be different if a more complete array of data available in the North Atlantic region had been considered. We argue that the strength of AMOC over recent centuries is still poorly constrained and the expected slowdown may not have started yet.K.H.K. acknowledges funding from NOAA grant NA20OAR4310481. D.E.A. and B.L.O.-B. acknowledge support from the National Center for Atmospheric Research, which is a major facility sponsored by the National Science Foundation under cooperative agreement no. 1852977. N.M.W. acknowledges support from a NOAA Climate and Global Change Postdoctoral Fellowship. M.F.J. acknowledges support from NSF award OCE-1846821 and C.M.L. acknowledges support from NSF award OCE-1805029. This is UMCES contribution 6062.Peer ReviewedArticle signat per 17 autors/es: University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, Solomons, MD, USA: K. Halimeda Kilbourne / Department of Geological and Atmospheric Sciences, Iowa State University, Ames, IA, USA: Alan D. Wanamaker / Geography Department, Durham University, Durham, UK: Paola Moffa-Sanchez / Centre for Geography and Environmental Sciences, University of Exeter, Penryn, UK: David J. Reynolds, Paul G. Butler & James Scourse / Climate and Global Dynamics Laboratory, National Center for Atmospheric Research, Boulder, CO, USA: Daniel E. Amrhein & Bette L. Otto-Bliesner / Woods Hole Oceanographic Institution, Falmouth, MA, USA: Geoffrey Gebbie & Nina M. Whitney / Cooperative Institute for Marine and Atmospheric Studies, University of Miami, Miami, FL, USA: Marlos Goes / Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration, Miami, FL, USA: Marlos Goes / Department of the Geophysical Sciences, The University of Chicago, Chicago, IL, USA: Malte F. Jansen / Oceanography Department, Atmospheric and Environmental Research, Inc., Texas, TX, USA: Christopher M. Little / US Geological Survey, St Petersburg Coastal and Marine Science Center, St Petersburg, FL, USA: Madelyn Mette / Barcelona Supercomputing Center, Barcelona, Spain: Eduardo Moreno-Chamarro & Pablo Ortega / Graduate School of Oceanography, University of Rhode Island, Kingston, RI, USA: Thomas Rossby / University Corporation of Atmospheric Research, Boulder, CO, USA: Nina M. WhitneyPostprint (author's final draft)Matters Arising published on 17 February 2022. The Original Article was published on 25 February 2021

Identification of the protein kinases Pyk3 and Phg2 as regulators of the STATc-mediated response to hyperosmolarity

Cellular adaptation to changes in environmental osmolarity is crucial for cell survival. In Dictyostelium, STATc is a key regulator of the transcriptional response to hyperosmotic stress. Its phosphorylation and consequent activation is controlled by two signaling branches, one cGMP- and the other Ca(2+)-dependent, of which many signaling components have yet to be identified. The STATc stress signalling pathway feeds back on itself by upregulating the expression of STATc and STATc-regulated genes. Based on microarray studies we chose two tyrosine-kinase like proteins, Pyk3 and Phg2, as possible modulators of STATc phosphorylation and generated single and double knock-out mutants to them. Transcriptional regulation of STATc and STATc dependent genes was disturbed in pyk3(-), phg2(-), and pyk3(-)/phg2(-) cells. The absence of Pyk3 and/or Phg2 resulted in diminished or completely abolished increased transcription of STATc dependent genes in response to sorbitol, 8-Br-cGMP and the Ca(2+) liberator BHQ. Also, phospho-STATc levels were significantly reduced in pyk3(-) and phg2(-) cells and even further decreased in pyk3(-)/phg2(-) cells. The reduced phosphorylation was mirrored by a significant delay in nuclear translocation of GFP-STATc. The protein tyrosine phosphatase 3 (PTP3), which dephosphorylates and inhibits STATc, is inhibited by stress-induced phosphorylation on S448 and S747. Use of phosphoserine specific antibodies showed that Phg2 but not Pyk3 is involved in the phosphorylation of PTP3 on S747. In pull-down assays Phg2 and PTP3 interact directly, suggesting that Phg2 phosphorylates PTP3 on S747 in vivo. Phosphorylation of S448 was unchanged in phg2(-) cells. We show that Phg2 and an, as yet unknown, S448 protein kinase are responsible for PTP3 phosphorylation and hence its inhibition, and that Pyk3 is involved in the regulation of STATc by either directly or indirectly activating it. Our results add further complexities to the regulation of STATc, which presumably ensure its optimal activation in response to different environmental cues

Paleophysical Oceanography with an Emphasis on Transport Rates

Paleophysical oceanography is the study of the behavior of the fluid ocean of the past, with a specific emphasis on its climate implications, leading to a focus on the general circulation. Even if the circulation is not of primary concern, heavy reliance on deep-sea cores for past climate information means that knowledge of the oceanic state when the sediments were laid down is a necessity. Like the modern problem, paleoceanography depends heavily on observations, and central difficulties lie with the very limited data types and coverage that are, and perhaps ever will be, available. An approximate separation can be made into static descriptors of the circulation (e.g., its water-mass properties and volumes) and the more difficult problem of determining transport rates of mass and other properties. Determination of the circulation of the Last Glacial Maximum is used to outline some of the main challenges to progress. Apart from sampling issues, major difficulties lie with physical interpretation of the proxies, transferring core depths to an accurate timescale (the “age-model problem”), and understanding the accuracy of time-stepping oceanic or coupled-climate models when run unconstrained by observations. Despite the existence of many plausible explanatory scenarios, few features of the paleocirculation in any period are yet known with certainty.National Science Foundation (U.S.) (grant OCE-0645936