La Parada. Un pódcast sobre paisatge urbà i históric de Barcelona mentre s'espera el bus

Màster Oficial en Gestió del Patrimoni Cultural i Museologia, Facultat de Geografia i Història, Universitat de Barcelona. Curs: 2022-2023. Tutor: Mònica MaspochEn segon lloc, presentem el marc pràctic, el qual s’estructura en tres grans subapartats que corresponen a: • Fonamentació i conceptualització. En aquest apartat es planteja la proposta i es presenten els objectius, seguit del desenvolupament de les línies del pla de treball per a l’execució del projecte. • Primera temporada. En aquest punt es duu a terme desenvolupament pràctic de la proposta amb la producció parcial d’una primera temporada del programa on s’estableixen les deu localitzacions, es realitzen els preguions basats en eixos temàtics, s’elabora el guió d’un programa i es grava un episodi pilot. • Implementació. En aquest àmbit s’estableix el model de gestió, els recursos i l’administració econòmica i temporal del pòdcast. Finalment, el projecte tanca amb unes conclusions i, a continuació, tota la bibliografia i webgrafia emprada per a l’elaboració de La Parada, l’índex de figures i un apartat de deu Annexos que sostenen tot el procés de creació dels preguions dels episodis del pòdcast de la primera temporada, definits com la fase de documentació

Gemini multi-conjugate adaptive optics system review II: Commissioning, operation and overall performance

The Gemini Multi-conjugate Adaptive Optics System - GeMS, a facility instrument mounted on the Gemini South telescope, delivers a uniform, near diffraction limited images at near infrared wavelengths (0.95 microns- 2.5 microns) over a field of view of 120 arc seconds. GeMS is the first sodium layer based multi laser guide star adaptive optics system used in astronomy. It uses five laser guide stars distributed on a 60 arc seconds square constellation to measure for atmospheric distortions and two deformable mirrors to compensate for it. In this paper, the second devoted to describe the GeMS project, we present the commissioning, overall performance and operational scheme of GeMS. Performance of each sub-system is derived from the commissioning results. The typical image quality, expressed in full with half maximum, Strehl ratios and variations over the field delivered by the system are then described. A discussion of the main contributor to performance limitation is carried-out. Finally, overheads and future system upgrades are described.Comment: 20 pages, 11 figures, accepted for publication in MNRA

Vibration measurement and mount design for cryocoolers on GMT and large telescopes

Cryocoolers have long been demonstrated to be a dominant source of vibration that have caused significant problems with AO systems on large telescopes. Existing large telescopes have already imposed strict vibration requirements on instruments in response to existing problems, and have often struggled to achieve them. As the field moves into the next generation telescopes with GMT, TMT and eELT, vibration requirements continue to get ever tighter. Instrument teams must respond to these more demanding requirements by careful selection of cryocoolers and thoughtful design of cryocooler mounts that are matched closely with the specific requirements of the telescope. As we will demonstrate in this paper there is not a one-size-fits-all solution for every instrument and every telescope. In this paper we demonstrate a general method of deriving the required performance for an anti-vibration mounts for cryocoolers. First we characterize a linear Stirling-type cryocooler as a source of vibration, and determine what compliant mounts would be required to make them acceptable for use on the VLT, GMT and TMT. Measurements are taken of vibration from a Cryotel GT linear Stirling cooler (with active vibration cancellation enabled). By comparing the measured vibration against the requirements of each telescope, we are able to determine the required transfer function and therefore the required spring rate for compliant mounts. The results indicate that while some simple rubber mounts may be sufficient for use with the VLT and TMT, but a compliant mount with natural frequency below 14 Hz must be used for GMT

A conceptual design study for Subaru ULTIMATE GLAO

We report on the conceptual design study done for the Ground Layer Adaptive Optics system of the ULTIMATE-Subaru project. This is an ambitious instrument project, providing GLAO correction in a square field of view of 14 arcmin on a side, aiming to deliver improved seeing at the near infrared wavelength. Its client instruments are an imager and multi-IFU spectrograph at Cassegrain and a Multi-Object spectrograph at Nasmyth. In this paper, we introduce the ULTIMATE-Subaru project overview and its science case and report the results of the GLAO performance prediction based on the numerical simulation and conceptual design of the wavefront sensor systemThe development of ULTIMATE-Subaru is partly supported by the Japan Society for the Promotion of Science (Grant-in-Aid for Research #17H06129)

Gemini multiconjugate adaptive optics system review - I. Design, trade-offs and integration

The Gemini multiconjugate adaptive optics system (GeMS) at the Gemini South telescope in Cerro Pachón is the first sodium-based multilaser guide star (LGS) adaptive optics system. It uses five LGSs and two deformable mirrors to measure and compensate fo

Molecular profiling and feasibility using a comprehensive hybrid capture panel on a consecutive series of non-small-cell lung cancer patients from a single centre

Background: Targeted next-generation sequencing (NGS) is recommended to screen actionable genomic alterations (GAs) in patients with non-small-cell lung cancer (NSCLC). We determined the feasibility to detect actionable GAs using TruSight™ Oncology 500 (TSO500) in 200 consecutive patients with NSCLC. Materials and methods: DNA and RNA were sequenced on an Illumina® NextSeq 550 instrument and processed using the TSO500 Docker pipeline. Clinical actionability was defined within the molecular tumour board following European Society for Medical Oncology (ESMO) guidelines for oncogene-addicted NSCLC. Overall survival (OS) was estimated as per the presence of druggable GAs and treatment with targeted therapy. Results: Most patients were males (69.5%) and former or current smokers (86.5%). Median age was 64 years. The most common histological type and tumour stage were lung adenocarcinoma (81%) and stage IV (64%), respectively. Sequencing was feasible in most patients (93.5%) and actionable GAs were found in 26.5% of patients. A high concordance was observed between single-gene testing and TSO500 NGS panel. Patients harbouring druggable GAs and receiving targeted therapy achieved longer OS compared to patients without druggable GAs. Conversely, patients with druggable GAs not receiving targeted therapy had a trend toward shorter OS compared with driver-negative patients. Conclusions: Hybrid capture sequencing using TSO500 panel is feasible to analyse clinical samples from patients with NSCLC and is an efficient tool for screening actionable GAs

Benchmarking of Whole Exome Sequencing and Ad Hoc Designed Panels for Genetic Testing of Hereditary Cancer

Next generation sequencing panels have been developed for hereditary cancer, although there is some debate about their cost-effectiveness compared to exome sequencing. The performance of two panels is compared to exome sequencing. Twenty-four patients were selected: ten with identified mutations (control set) and fourteen suspicious of hereditary cancer but with no mutation (discovery set). TruSight Cancer (94 genes) and a custom panel (122 genes) were assessed alongside exome sequencing. Eightythree genes were targeted by the two panels and exome sequencing. More than 99% of bases had a read depth of over 30x in the panels, whereas exome sequencing covered 94%. Variant calling with standard settings identified the 10 mutations in the control set, with the exception of MSH6 c.255dupC using TruSight Cancer. In the discovery set, 240 unique non-silent coding and canonic splice-site variants were identified in the panel genes, 7 of them putatively pathogenic (in ATM, BARD1, CHEK2, ERCC3, FANCL, FANCM, MSH2). The three approaches identified a similar number of variants in the shared genes. Exomes were more expensive than panels but provided additional data. In terms of cost and depth, panels are a suitable option for genetic diagnostics, although exomes also identify variants in non-targeted genes

Efficacy of CDK4/6 inhibitors in preclinical models of malignant pleural mesothelioma

Background There is no effective therapy for patients with malignant pleural mesothelioma (MPM) who progressed to platinum-based chemotherapy and immunotherapy. Methods We aimed to investigate the antitumor activity of CDK4/6 inhibitors using in vitro and in vivo preclinical models of MPM. Results Based on publicly available transcriptomic data of MPM, patients with CDK4 or CDK6 overexpression had shorter overall survival. Treatment with abemaciclib or palbociclib at 100 nM significantly decreased cell proliferation in all cell models evaluated. Both CDK4/6 inhibitors significantly induced G1 cell cycle arrest, thereby increasing cell senescence and increased the expression of interferon signalling pathway and tumour antigen presentation process in culture models of MPM. In vivo preclinical studies showed that palbociclib significantly reduced tumour growth and prolonged overall survival using distinct xenograft models of MPM implanted in athymic mice. Conclusions Treatment of MPM with CDK4/6 inhibitors decreased cell proliferation, mainly by promoting cell cycle arrest at G1 and by induction of cell senescence. Our preclinical studies provide evidence for evaluating CDK4/6 inhibitors in the clinic for the treatment of MPM