Shipboard Crisis Management: A Case Study.

The loss of the "Green Lily" in 1997 is used as a case study to highlight the characteristics of escalating crises. As in similar safety critical industries, these situations are unpredictable events that may require co-ordinated but flexible and creative responses from individuals and teams working in stressful conditions. Fundamental skill requirements for crisis management are situational awareness and decision making. This paper reviews the naturalistic decision making (NDM) model for insights into the nature of these skills and considers the optimal training regimes to cultivate them. The paper concludes with a review of the issues regarding the assessment of crisis management skills and current research into the determination of behavioural markers for measuring competence

Can Cognitive Science Improve the Training of Industrial Process Operators?

The work tasks of industrial process operators can have far reaching implications for both the safety of personnel and protection of the environment. The training of these operators to be competent in their work tasks, therefore, attains a high level of importance. The control of industrial processes often requires operators to undertake complex dynamic tasks. Cognitive science is attempting to explain the cognitive processes that underlie the behaviour of operators when carrying out these tasks. This paper will investigate the current theories concerning these cognitive processes and will discuss their implications toward the training of industrial process operators

Are Current Risk Management Strategies within the Commercial Shipping Industry Adequate?

Commercial shipping is a high-risk industry and as such should be actively engaged in the management of risk in order to reduce the number of incidents which both threaten the safety of seafarers and the protection of the environment. The high number of incidents within the commercial shipping industry raises the question of the adequacy of current risk management strategies within the industry. This paper discusses these strategies and relates them to current risk management practices in order to propose possibilities for their improvement

REF1/Aly and the additional exon junction complex proteins are dispensable for nuclear mRNA export

The metazoan proteins UAP56, REF1, and NXF1 are thought to bind sequentially to mRNA to promote its export to the cytoplasm: UAP56 is thought to recruit REF1 to nascent mRNA; REF1 acts as an adaptor protein mediating the association of NXF1 with mRNA, whereas NXF1 translocates the mRNA across the nuclear pore complex. REF1 is a component of the exon–exon junction complex (EJC); thus, the EJC is thought to play a role in the export of spliced mRNA. NXF1 and UAP56 are essential for mRNA export. An essential role for metazoan REF1 or the additional EJC proteins in this process has not been established. Contrary to expectation, we show that REF1 and the additional components of the EJC are dispensable for export of bulk mRNA in Drosophila cells. Only when REF1 and RNPS1 are codepleted, or when all EJC proteins are simultaneously depleted is a partial nuclear accumulation of polyadenylated RNAs observed. Because a significant fraction of bulk mRNA is detected in the cytoplasm of cells depleted of all EJC proteins, we conclude that additional adaptor protein(s) mediate the interaction between NXF1 and cellular mRNAs in metazoa. Our results imply that the essential role of UAP56 in mRNA export is not restricted to the recruitment of REF1

A Research Agenda in Maritime Crew Resource Management.

This paper opens with a brief introduction to the development of Crew Resource Management (CRM) training in the international shipping industry, a concept that was first advanced through the use of simulators in maritime training colleges over 25 years ago. The paper charts the development of the shipping industry’s approach to the preparation of bridge and engine room teams for normal and abnormal operations, and critiques the current training regime in resource management. Two case studies are presented to highlight some of the CRM issues raised by recent maritime casualties, and the paper then proceeds to set out a research agenda for exploring some of these issues. The paper provides an overview of three research initiatives: the first is to gain a better theoretical understanding of the nature of shared situational awareness and mental models in "real world" maritime operations. A second initiative is to identify a set of behavioural markers for assessing the non-technical skills of crisis management. The third initiative is to explore the role of organisational factors in safe operation, in recognition of the limitations of operator training as a panacea to prevent the re-occurrence of accidents

Behavioural markers for the assessment of competence in crisis management

A lack of competence in crisis management has been shown to be a causal factor in a number of recent maritime accidents. In safety critical industries other than commercial shipping, such as civil aviation, nuclear and petrochemical, research is being undertaken to identify behavioural markers that can be used to assess competence in crisis management. Although there is now a general acceptance of the core concepts for the non-technical or resource management skills required for competence in crisis management, there is also an acceptance that the behaviours associated with these skills are context specific. This research programme improves the understanding of how a behavioural marker system can be used to assess the competence in crisis management of merchant marine engineering officers within the context of a merchant vessel engine control room. This research reviews the current practice in using behavioural markers for the assessment of competence in crisis management within safety critical industries and the military. The differences between the assessment frameworks and environments in which behavioural markers are currently being used for this assessment of competence are discussed. The influences of these differences on the use of behavioural markers for the assessment of competence in crisis management within the context of a merchant vessel engine room control room are investigated. Through the use of ethnographic study, the research presents a set of behavioural markers that can be used to assess competence in crisis management within the context of a simulated merchant vessel's engine room control room. The research concludes that these behavioural markers can be used as a valid objective assessment framework for the assessment of ocompetence in crisis management of merchant navy engineering officers

THE USE OF LINKED SIMULATORS IN PROJECT “HORIZON”: RESEARCH INTO SEAFARER FATIGUE

This paper will provide an overview of Project HORIZON, and review some of the scientific findings on the effects of fatigue on seafarers, as well as the experience of using simulators for major research projects. The HORIZON project is a European Framework 7 sponsored research project to investigate the effects of fatigue on the cognitive performance of bridge and engine room watch keepers during a 7 day simulated voyage, using a range of linked simulators and under different watch patterns and conditions of workload. During the experimental period, measurements were taken of their watch keeping performance and their levels of alertness, including the use of EEG recording. A total of 90 certificated seafarers, with varying levels of experience, have undertaken the simulated voyages. It is hoped that the findings from this project will lead to more effective Fatigue Risk Management Systems (FRMS), in order to improve the safety of ship operations and the welfare of seafarers. The paper will discuss these findings and the lessons learned for future simulator research projects

MicroRNA-122 Modulates the Rhythmic Expression Profile of the Circadian Deadenylase Nocturnin in Mouse Liver

Nocturnin is a circadian clock-regulated deadenylase thought to control mRNA expression post-transcriptionally through poly(A) tail removal. The expression of Nocturnin is robustly rhythmic in liver at both the mRNA and protein levels, and mice lacking Nocturnin are resistant to diet-induced obesity and hepatic steatosis. Here we report that Nocturnin expression is regulated by microRNA-122 (miR-122), a liver specific miRNA. We found that the 3′-untranslated region (3′-UTR) of Nocturnin mRNA harbors one putative recognition site for miR-122, and this site is conserved among mammals. Using a luciferase reporter construct with wild-type or mutant Nocturnin 3′-UTR sequence, we demonstrated that overexpression of miR-122 can down-regulate luciferase activity levels and that this effect is dependent on the presence of the putative miR-122 recognition site. Additionally, the use of an antisense oligonucleotide to knock down miR-122 in vivo resulted in significant up-regulation of both Nocturnin mRNA and protein expression in mouse liver during the night, resulting in Nocturnin rhythms with increased amplitude. Together, these data demonstrate that the normal rhythmic profile of Nocturnin expression in liver is shaped in part by miR-122. Previous studies have implicated Nocturnin and miR-122 as important post-transcriptional regulators of both lipid metabolism and circadian clock controlled gene expression in the liver. Therefore, the demonstration that miR-122 plays a role in regulating Nocturnin expression suggests that this may be an important intersection between hepatic metabolic and circadian control

Coronin 1C harbours a second actin-binding site that confers co-operative binding to F-actin

Dynamic rearrangement of actin filament networks is critical for cell motility, phagocytosis and endocytosis. Coronins facilitate these processes, in part, by their ability to bind F-actin (filamentous actin). We previously identified a conserved surface-exposed arginine (Arg30) in the β-propeller of Coronin 1B required for F-actin binding in vitro and in vivo. However, whether this finding translates to other coronins has not been well defined. Using quantitative actin-binding assays, we show that mutating the equivalent residue abolishes F-actin binding in Coronin 1A, but not Coronin 1C. By mutagenesis and biochemical competition, we have identified a second actin-binding site in the unique region of Coronin 1C. Interestingly, leading-edge localization of Coronin 1C in fibroblasts requires the conserved site in the β-propeller, but not the site in the unique region. Furthermore, in contrast with Coronin 1A and Coronin 1B, Coronin 1C displays highly co-operative binding to actin filaments. In the present study, we highlight a novel mode of coronin regulation, which has implications for how coronins orchestrate cytoskeletal dynamics

Intron retention in the Drosophila melanogaster Rieske iron sulphur protein gene generated a new protein

Genomes can encode a variety of proteins with unrelated architectures and activities. It is known that protein-coding genes of de novo origin have significantly contributed to this diversity. However, the molecular mechanisms and evolutionary processes behind these originations are still poorly understood. Here we show that the last 102 codons of a novel gene, Noble, assembled directly from non-coding DNA following an intronic deletion that induced alternative intron retention at the Drosophila melanogaster Rieske Iron Sulphur Protein (RFeSP) locus. A systematic analysis of the evolutionary processes behind the origin of Noble showed that its emergence was strongly biased by natural selection on and around the RFeSP locus. Noble mRNA is shown to encode a bona fide protein that lacks an iron sulphur domain and localizes to mitochondria. Together, these results demonstrate the generation of a novel protein at a naturally selected site