Desarrollo de sistema de defensa contra las heladas en cultivos de almendras

La presente tesina plantea el diseño teórico de un software para la defensa contra las heladas en cultivos de almendras, y su implementación en una finca en la provincia de Mendoza, el cual está conformado por sensores, central donde estará la PC que guardará los datos y PC clientes que accederán a la información. El objetivo de este sistema, es garantizar el almacenamiento de la información para poder ser accedidos desde una PC o dispositivo móvil, enviando alertas en momentos en los que se considere una temperatura crítica. Se desarrollarán los contenidos teóricos de las tecnologías disponibles en el mercado para la realización del software, se seleccionan las más adecuadas para la implementación, como el tipo de lenguaje, base de datos y protocolos de comunicación. Por último, se describen características funcionales, configuraciones necesarias para el funcionamiento y, mediante imágenes, se explica el proceso de ejecución con las opciones que dará al usuario el sistema desarrollado.Fil: Garro, María Fernand

Detection of a Williams Beuren syndrome case by MLPA.

El síndrome de Williams-Beuren (WBS) es un trastorno del desarrollo neurológico que incluye diferentes manifestaciones clínicas como estenosis aórtica supravalvular, lesiones cerebrovasculares, retraso en el crecimiento, rasgos faciales "élficos" y retraso mental. Es causado por una microdeleción heterocigótica de genes contiguos en la banda cromosómica 7q11.23, generando un cambio en el número de copias (CNV) de esta región crítica. Los pacientes presentan una amplia manifestación clínica y variada expresión fenotípica. La confirmación de la sospecha clínica es esencial para el seguimiento clínico del paciente y el asesoramiento genético de la familia. La técnica estándar para la detección de WBS es la hibridización fluorescente in situ. En los últimos años la metodología MLPA (Multiplex Ligation dependent Probe Amplification) ha sido incorporada a los laboratorios diagnósticos para la detección de CNV relacionados con distintas enfermedades, incluyendo WBS. El objetivo de este trabajo fue confirmar el diagnóstico clínico de WBS en un niño, utilizando la técnica de MLPA. Los ensayos por MLPA permitieron detectar la deleción de los genes CYLN2, FZD9, STX1A, ELN, LIMK1y RFC2. En regiones geográficas donde la determinación por FISH (Fluorescence In Situ Hybridization) no está disponible para esta enfermedad, la metodología MLPA ha permitido confirmar el diagnóstico clínico y detectar los genes involucrados en la alteración. Hasta nuestro conocimiento no hay otros casos publicados sobre síndrome de WB detectado por la técnica MLPA en la ArgentinaDetection of a Williams Beuren syndrome case by MLPA. Williams-Beuren syndrome (WBS) is a rare developmental disorder characterized by distinctive facial, neurobehavioral, and cardiovas- cular features. WBS is caused by a heterozygous contiguous gene microdeletion of the WBS crítical region on chromosome 7q11.23. Confirmation of clinical suspicion is essential for clinical monitoring of the patient and genetic counseling of the family. Fluorescence in situ hybridization (FISH) is considered the gold standard technique for detecting WBS. Multiplex ligation-dependent probe amplification (MLPA) has been introduced into DNA diagnostic laboratories for the detection of copy number variations in several diseases including WBS. The objective of this study was to confirm, by MLPA, the clinical diagnosis of WBS in a pediatric patient. This technique allowed to detect the deletion of CYLN2, FZD9, STX1A, ELN, LIMK1 and RFC2 genes. In geographic regions were the detection by FISH is not available for this disease, the MLPA methodology allowed to confirm the clinic diagnostic of WBS. To our knowledge this is the first report demonstrating the confirmation of WBS by MLPA in Argentina.Fil: Laurito, Sergio Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Mendoza. Instituto Histologia y Embriologia de Mendoza; Argentina;Fil: Branham, Maria Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Mendoza. Instituto Histologia y Embriologia de Mendoza; Argentina;Fil: Herrero, Gustavo. Pediátrica San Luis Centro Médico Privado; Argentina;Fil: Marsá, Silvana. Pediátrica San Luis Centro Médico Privado; Argentina;Fil: Garro, Fernanda. Pediátrica San Luis Centro Médico Privado; Argentina;Fil: Roque Moreno, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Mendoza. Instituto Histologia y Embriologia de Mendoza; Argentina

Influencia del embarazo en la fisiología de la deportista élite: una revisión narrativa

The aim of this literature review is to determine the influence of pregnancy on the physiology of the elite female athlete. A review of scientific articles applied research and literature that provided information on cardiovascular, respiratory, musculoskeletal and endocrine changes in pregnant elite athletes in databases such as Google Scholar, PubMed, Scopus, Web of Science (WOS) and Science Direct was taken into account. Using the PICO strategy, the key words were determined to filter the information, and the 62 most relevant articles and 4 books were selected, concluding that research on pregnant elite sportswomen is still little studied, and that it is convenient to know in depth the adaptations to elite sport and pregnancy in female physiology and their influence on sports performance, and that it is necessary to expand with future research.El objetivo de esta revisión bibliográfica es determinar la influencia del embarazo en la fisiología de la mujer deportista élite. Se tuvo en cuenta la revisión de artículos científicos, investigaciones aplicadas y literatura que proporcionara información sobre los cambios cardiovasculares, respiratorios, musculoesqueléticos y endocrinos de la deportista élite gestante en bases de datos como Google Scholar, PubMed, Scopus, Web of Science (WOS) y Science Direct. A través de la estrategia PICO se determinaron las palabras clave para filtrar la información, se seleccionaron los 62 artículos y 4 libros más relevantes concluyendo que la investigación en las deportistas élite embarazadas es aún poco estudiado, siendo conveniente conocer a profundidad las adaptaciones al deporte élite y al embarazo en la fisiología femenina y su influencia en el rendimiento deportivo, siendo necesario ampliar con investigaciones futuras.&nbsp

Genomic Ancestry, CYP2D6, CYP2C9, and CYP2C19 Among Latin Americans

We present the distribution of CYP2D6, CYP2C9, and CYP2C19 variants and predicted phenotypes in 33 native and admixed populations from Ibero-America (n > 6,000) in the context of genetic ancestry (n = 3,387). Continental ancestries are the major determinants of frequencies of the increased-activity allele CYP2C19*17 and CYP2C19 gUMs (negatively associated with Native American ancestry), decreased-activity alleles CYP2D6*41 and CYP2C9*2 (positively associated with European ancestry), and decreased-activity alleles CYP2D6*17 and CYP2D6*29 (positively associated with African ancestry). For the rare alleles, CYP2C9*2 and CYPC19*17, European admixture accounts for their presence in Native American populations, but rare alleles CYP2D6*5 (null-activity), CYP2D6-multiplication alleles (increased activity), and CYP2C9*3 (decreased-activity) were present in the pre-Columbian Americas. The study of a broad spectrum of Native American populations from different ethno-linguistic groups show how autochthonous diversity shaped the distribution of pharmaco-alleles and give insights on the prevalence of clinically relevant phenotypes associated with drugs, such as paroxetine, tamoxifen, warfarin, and clopidogrel

Learn to Read with ICT

El trabajo de grado describe una experiencia docente realizada con un grupo de estudiantes que tiene como objetivo fortalecer la habilidad lectora a través del uso de los recursos tecnológicos existentes en el aula, creando un EVA en la plataforma Moodle, con actividades enfocadas hacia la comprensión de diferentes tipos de lectura relacionadas con su entorno próximo. Los estudiantes estuvieron acompañados por Rusty, un personaje de la televisión que se destaca por ser inventor y ayudar al planeta, el cual se hizo partícipe durante el proceso como estrategia pedagógica para acercarlos a la lectura, desarrollar competencias y así mismo ser autónomos, fortalecer el trabajo colaborativo, apropiarse e impartir el conocimiento con pares y docentes en diversos contextos, comparando los procesos iniciales con los finales los cuales describen el proceso de las habilidades adquiridas.The investigation describes a teaching experience made with a group of students that aims to strengthen reading ability through the use of existing technological resources in the classroom, creating an VLE on the Moodle platform, with activities focused on comprehension different types reading related to your immediate environment. The students were accompanied by Rusty, a television character who stands out for being an inventor and helping the planet, who became a participant during the process as a pedagogical strategy to bring them closer to reading, develop skills and also be autonomous, strengthen collaborative work, appropriate and impart knowledge with classmates and teachers in various contexts, comparing the initial processes with the final which describe the process of acquired skills.Secretaría de Educación de Bogot

Infantile spasms in Down syndrome: a good response to vitamin B6

Abstract We report the results of patients with Down syndrome (DS) and West syndrome (WS) treated with vitamin B6 and discuss the mechanisms of pyridoxine in WS. Twenty patients with DS and WS were referred to our service for infantile spasms (IS) between February 1990 and December 2002. WS was diagnosed on the basis of clinical and electroencephalogram features. Thirteen patients were treated conservately while seven patients (four girls and three boy) were treated according to the new alternative treatment scheme with vitamine B6. The dose of oral pyridoxine was between 200 and 400 mg/day (25-50 mg/kg/day). Five patients initiated the treatment with vitamin B6 for IS two weeks and two patients four weeks after starting to receive antiepileptic drugs. One of them, who initially received intravenous pyridoxine 200 mg/day during three days with a partial control of IS, responded well to oral vitamin B6 400 mg/day associated to valproic acid five months later. Mean age at the beginning of pyridoxine treatment was 9.5 months, ranging from 3 to 14 months. The time between the onset of spasms and vitamin B6 administration ranged from 2 to 4 weeks. Cessation of spasms was obtained within 2 weeks of treatment in four patients. Their electroencephalograms became normal as soon as the spasms disappeared. In four patients whose spasms ceased, pyridoxine was discontinued after 24 and 30 months and clinical spasms did not recur. These four children did not experienceany other type of seizure after a mean time of follow-up of 6 years (range 1-10 years) without any type of treatment. In the fifth patient, cessation of spasms was obtained within four weeks the second time she received vitamin B6, five months after the first treatment. She still receives with pyridoxine 400 mg/day (40 mg/kg/day) after twelve months of follow up and has had no spasms since. Two patients did not respond well either to pyridoxine or to vigabatrin, adrenocorticotropic hormone, valproic acid or benzodiazepines. The patients had no adverse events during treatment with pyridoxine. We consider an oral dose of 200-400 mg/day (25-50 mg/kg/day) of pyridoxine either in monotherapy or combined with classic antiepleptic drugs to be the first choice of therapy in patients with IS and DS. Furthermore, patients who initially do not respond well to pyridoxine could be treated again and for a longer period. (J Pediatr Neurol 2004; 2(1): 15-19)