The complete plastome of Hyacinthoides non-scripta (L.) Chouard ex Rothm. (Asparagaceae)

The whole plastome sequence ofHyacinthoides non-scripta, was assembled and annotated in this study.This is the first complete plastid genome for the genusHyacinthoides. The plastome is 155,035 bp longand consists of a large single-copy (LSC) region spanning 83,947 bp, a small single-copy (SSC) regionspanning 18,496bp, and two inverted repeat (IR) regions, each of which is 26,296bp in length. Thereare 132 genes annotated in the plastome, of which the protein-coding geneinfAhas pseudogenized

Conceptual design study for an advanced cab and visual system, volume 2

The performance, design, construction and testing requirements are defined for developing an advanced cab and visual system. The rotorcraft system integration simulator is composed of the advanced cab and visual system and the rotorcraft system motion generator, and is part of an existing simulation facility. User's applications for the simulator include rotorcraft design development, product improvement, threat assessment, and accident investigation

Tunneling mechanism of light transmission through metallic films

A mechanism of light transmission through metallic films is proposed, assisted by tunnelling between resonating buried dielectric inclusions. This is illustrated by arrays of Si spheres embedded in Ag. Strong transmission peaks are observed near the Mie resonances of the spheres. The interaction among various planes of spheres and interference effects between these resonances and the surface plasmons of Ag lead to mixing and splitting of the resonances. Transmission is proved to be limited only by absorption. For small spheres, the effective dielectric constant can be tuned to values close to unity and a method is proposed to turn the resulting materials invisible.Comment: 4 papges, 5 figure

Chemical abundances in spiral and irregular galaxies. O and N abundances derived from global emission--line spectra

The validity of oxygen and nitrogen abundances derived from the global emission-line spectra of galaxies via the P-method has been investigated using a collection of published spectra of individual HII regions in irregular and spiral galaxies. The conclusions of Kobulnicky, Kennicutt & Pizagno (1999) that global emission-line spectra can reliably indicate the chemical properties of galaxies has been confirmed. It has been shown that the comparison of the global spectrum of a galaxy with a collection of spectra of individual HII regions can be used to distinguish high and low metallicity objects and to estimate accurate chemical abundances in a galaxy. The oxygen and nitrogen abundances in samples of UV-selected and normal nearby galaxies have been determined. It has been found that the UV-selected galaxies occupy the same area in the N/O -- O/H diagram as individual HII regions in nearby galaxies. Finally, we show that intermediate-redshift galaxies systematically deviate from the metallicity -- luminosity trend of local galaxies.Comment: 15 pages, 17 figures, accepted for publication in Astronomy and Astrophysic

Associations between smoking status and bodily pain in a cross-sectional survey of UK respondents

BACKGROUND: Research indicates that ex- and current smokers report increased levels of bodily pain compared with never smokers. This could be secondary to smoking-related disease or psychological characteristics of smokers, or it could be a neurological or vascular effect of a period of regular smoking. AIMS: We compared self-reported levels of bodily pain in daily, never daily and former daily smokers stratified by age group and adjusting for a wider range of covariates than has been undertaken to-date, including health status, neuroticism, anxiety and depression. METHOD: 223,537 UK respondents aged 16+ years were surveyed between 2009 and 2013 in the British Broadcasting Corporation (BBC) Lab UK Study. Respondents provided information on bodily pain, smoking status and a range of sociodemographic, health, behavioural and psychological characteristics. RESULTS: After adjusting for all covariates, in 16-34-year-olds, reported levels of bodily pain in former daily smokers (B_{adj} = 0.72, 95% CI = 0.30, 1.15, p < .001) and daily smokers (B_{adj} = 0.50, 95% CI = 0.18, 0.82, p < .01) were higher than in never daily smokers. Reported levels of bodily pain were also higher in former daily smokers than in never daily smokers in those aged 35–64 (B_{adj} = 1.04, 95% CI = 0.69, 1.38, p < .001) and 65 + years (B_{adj} = 1.65, 95% CI = 0.07, 3.24, p < .05). CONCLUSIONS: After adjusting for key characteristics, former daily smokers reported higher levels of bodily pain compared with never daily smokers at all ages. This raises the possibility that a period of smoking may have lasting effects on pain experiences

Potential explanations for conflicting findings on abrupt versus gradual smoking cessation: a population study in England

BACKGROUND AND AIM: Observational and trial evidence conflict on the efficacy of two contrasting behavioural approaches to quitting smoking – gradual and abrupt. Observational data suggests an abrupt approach to quitting is superior to a gradual approach, whilst trials show no difference. One potential explanation is self-selection in observational data, whereby people can choose their quit approach and those who find it harder to quit may be more likely to choose a gradual quit approach. This study aims to investigate potential explanations for these conflicting findings. METHODS: We used observational data from a nationally representative sample of adults in England from November 2006 to February 2020 who reported smoking and had made at least one quit attempt in the past year (n=21,542). We used logistic regression models to assess the association between abrupt versus gradual quit attempts and quit success, adjusting for sociodemographic, smoking and quit attempt characteristics. FINDINGS: Abrupt, versus gradual, attempts were associated with improved quit success in an unadjusted model (OR=2.02, 95% CI=1.86-2.19). This association remained after adjusting for a broad range of relevant confounders (OR=1.75, 95% CI=1.59-1.93). CONCLUSIONS: Among a representative sample of adults who had smoked and made a quit attempt in the past year, there was evidence of an association between abrupt attempts and quit success before and after adjusting for relevant confounders. This suggests that the differences in quit success seen between abrupt and gradual quit attempt types are not completely driven by self-selection in observational data. IMPLICATIONS: We investigated explanations for conflicting findings on the efficacy of gradual versus abrupt approaches to quitting smoking between trial and observational data. Despite adjusting observational data for sociodemographic, smoking and quit attempt characteristics, an association between abrupt quitting and quit success remained. Therefore, differences in quit success were not completely driven by self-selection of a gradual approach by people who found it especially difficult to quit or differences in the use of quitting aids. However, characteristics adjusted for were limited by the data available, and future research should continue to investigate the difference in findings across study types to inform cessation support

Association of the Covid-19 lockdown with smoking, drinking, and attempts to quit in England: an analysis of 2019-2020 data

Aim: To examine changes in smoking, drinking and quitting/reduction behaviour following the COVID‐19 lockdown in England. Design/setting: Monthly cross‐sectional surveys representative of the adult population in England, aggregated before (April 2019–February 2020) versus after (April 2020) lockdown. Participants: A total of 20 558 adults (≥ 16 years). Measurements: The independent variable was the timing of the COVID‐19 lockdown (before versus after March 2020). Dependent variables were: prevalence of smoking and high‐risk drinking, past‐year cessation and quit attempts (among past‐year smokers), past‐year attempts to reduce alcohol consumption (among high‐risk drinkers) and use of evidence‐based (e.g. prescription medication/face‐to‐face behavioural support) and remote support [telephone support/websites/applications (apps)] for smoking cessation and alcohol reduction (among smokers/high‐risk drinkers who made a quit/reduction attempt). Covariates included age, sex, social grade, region and level of nicotine and alcohol dependence (as relevant). Findings: The COVID‐19 lockdown was not associated with a significant change in smoking prevalence [17.0% (after) versus 15.9% (before), odds ratio (OR) = 1.09, 95% CI = 0.95–1.24], but was associated with increases in quit attempts [39.6 versus 29.1%, adjusted odds ratio (ORadj) = 1.56, 95% CI = 1.23–1.98], quit success (21.3 versus 13.9%, ORadj = 2.01, 95% CI = 1.22–3.33) and cessation (8.8 versus 4.1%, ORadj = 2.63, 95% CI = 1.69–4.09) among past‐year smokers. Among smokers who tried to quit, there was no significant change in use of evidence‐based support (50.0 versus 51.5%, ORadj = 1.10, 95% CI = 0.72–1.68) but use of remote support increased (10.9 versus 2.7%, ORadj = 3.59, 95% CI = 1.56–8.23). Lockdown was associated with increases in high‐risk drinking (38.3 versus 25.1%, OR = 1.85, CI = 1.67–2.06), but also alcohol reduction attempts by high‐risk drinkers (28.5 versus 15.3%, ORadj = 2.16, 95% CI = 1.77–2.64). Among high‐risk drinkers who made a reduction attempt, use of evidence‐based support decreased (1.2 versus 4.0%, ORadj = 0.23, 95% CI = 0.05–0.97) and there was no significant change in use of remote support (6.9 versus 6.1%, ORadj = 1.32, 95% CI = 0.64–2.75). Conclusions: Following the March 2020 COVID‐19 lockdown, smokers and high‐risk drinkers in England were more likely than before lockdown to report trying to quit smoking or reduce alcohol consumption and rates of smoking cessation and use of remote cessation support were higher. However, high‐risk drinking prevalence increased post‐lockdown and use of evidence‐based support for alcohol reduction by high‐risk drinkers decreased with no compensatory increase in use of remote support

Radiocarbon dating of methane and carbon dioxide evaded from a temperate peatland stream

Streams draining peatlands export large quantities of carbon in different chemical forms and are an important part of the carbon cycle. Radiocarbon (14C) analysis/dating provides unique information on the source and rate that carbon is cycled through ecosystems, as has recently been demonstrated at the air-water interface through analysis of carbon dioxide (CO2) lost from peatland streams by evasion (degassing). Peatland streams also have the potential to release large amounts of methane (CH4) and, though 14C analysis of CH4 emitted by ebullition (bubbling) has been previously reported, diffusive emissions have not. We describe methods that enable the 14C analysis of CH4 evaded from peatland streams. Using these methods, we investigated the 14C age and stable carbon isotope composition of both CH4 and CO2 evaded from a small peatland stream draining a temperate raised mire. Methane was aged between 1617-1987 years BP, and was much older than CO2 which had an age range of 303-521 years BP. Isotope mass balance modelling of the results indicated that the CO2 and CH4 evaded from the stream were derived from different source areas, with most evaded CO2 originating from younger layers located nearer the peat surface compared to CH4. The study demonstrates the insight that can be gained into peatland carbon cycling from a methodological development which enables dual isotope (14C and 13C) analysis of both CH4 and CO2 collected at the same time and in the same way

Distance Dependence in the Solar Neighborhood Age-Metallicity Relation

The age-metallicity relation for F and G dwarf stars in the solar neighborhood, based on the stellar metallicity data of Edvardsson et al. (1993), shows an apparent scatter that is larger than expected considering the uncertainties in metallicities and ages. A number of theoretical models have been put forward to explain the large scatter. However, we present evidence, based on Edvardsson et al. (1993) data, along with Hipparcos parallaxes and new age estimates, that the scatter in the age-metallicity relation depends on the distance to the stars in the sample, such that stars within 30 pc of the Sun show significantly less scatter in [Fe/H]. Stars of intermediate age from the Edvardsson et al. sample at distances 30-80 pc from the Sun are systematically more metal-poor than those more nearby. We also find that the slope of the apparent age-metallicity relation is different for stars within 30 pc than for those stars more distant. These results are most likely an artifact of selection biases in the Edvardsson et al. star sample. We conclude that the intrinsic dispersion in metallicity at fixed age is < 0.15 dex, consistent with the < 0.1 dex scatter for Galactic open star clusters and the interstellar medium.Comment: 15 pages, 5 figures, uses AASTex aaspp4 style; accepted for publication in the Astrophysical Journa

A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration.

Both lung disease and elevation of blood glucose are associated with increased glucose concentration (from 0.4 to ~4.0 mM) in the airway surface liquid (ASL). This perturbation of ASL glucose makes the airway more susceptible to infection by respiratory pathogens. ASL is minute (~1 μl/cm(2)) and the measurement of glucose concentration in the small volume ASL is extremely difficult. Therefore, we sought to develop a fluorescent biosensor with sufficient sensitivity to determine glucose concentrations in ASL in situ. We coupled a range of environmentally sensitive fluorophores to mutated forms of a glucose/galactose-binding protein (GBP) including H152C and H152C/A213R and determined their equilibrium binding properties. Of these, GBP H152C/A213R-BADAN (Kd 0.86 ± 0.01 mM, Fmax/F0 3.6) was optimal for glucose sensing and in ASL increased fluorescence when basolateral glucose concentration was raised from 1 to 20 mM. Moreover, interpolation of the data showed that the glucose concentration in ASL was increased, with results similar to that using glucose oxidase analysis. The fluorescence of GBP H152C/A213R-BADAN in native ASL from human airway epithelial cultures in situ was significantly increased over time when basolateral glucose was increased from 5 to 20 mM. Overall our data indicate that this GBP is a useful tool to monitor glucose homoeostasis in the lung