Dominance patterns of macrobenthic faunal groups at different depth levels in the sublittoral sandy marine sediments of the Ria de Ares y Betanzos (Galicia, northwest Iberian Peninsula)

Sublittoral sandy bottoms of the Ria de Ares y Betanzos (Galicia, northwest Iberian Peninsula) were studied to determine the dominance patterns of macrobenthic faunal groups at different depth levels in the marine sediment. Samples of 20-25 cm average depth and 0.012 m² surface were analysed, considering 5 faunal groups: polychaetes, molluscs, crustaceans, echinoderms, and others. Our abundance results show the highest values for polychaetes, mainly near the surface, which decline with penetration into the sediment, where molluscs and the others group reach important relative values.Se estudian los fondos arenosos sublitorales de la ría de Ares y Betanzos (noroeste de Galicia), con el objetivo de conocer los patrones de dominancia de los grupos faunísticos macrobentónicos a diferentes niveles de profundidad del sedimento marino. Se analizan muestras de superficie de 0,012 m² y 20-25 cm de profundidad media, considerándose cinco grupos faunísticos: poliquetos, moluscos, crustáceos, equinodermos y otros. Los poliquetos son el grupo faunístico más abundante, principalmente en los primeros centímetros de profundidad. A medida que se profundiza en el sedimento, los moluscos y el grupo otros adquieren valores relativos importantes.Instituto Español de Oceanografí

Minimum depth required for monitoring the impact of marine culture on benthic fauna

The macrobenthos is an useful tool for studying the impact of marine fish farms on the nearby area. Sampling macrobenthic fauna is both work- and time-intensive, so it is important to determine the minimum optimal volume to be sampled. For this purpose, the present study aimed to find the depth of samples needed to carry out an accurate monitoring of a bottom affected by fish farms. Two different types of bottom (sandy and silty) were sampled in two Galician rias (O Barqueiro and Vigo) in which there are fish farms. In the first 6 cm, we found 86.29 % of the species richness and 84.75 % of fauna density. The results obtained for population structure also show that 6 cm depth samples can be adequate for these kinds of studies.La obtención de la macrofauna bentónica (muestreo, separación e identificación) para evaluar el impacto provocado por los cultivos marinos resulta muy laboriosa y requiere mucho tiempo. Para ahorrar tiempo y esfuerzo, sería de gran ayuda trabajar con un volumen mínimo de muestra. Con este objetivo, este estudio pretende hallar la profundidad mínima que deben tener las muestras para poder llevar a cabo un adecuado seguimiento de los fondos afectados por cultivos marinos. Se muestrearon dos tipos de fondo (arenoso y fangoso) en dos rías gallegas (O Barqueiro y Vigo). En los primeros 6 cm del sedimento se recogió el 86,29 % de la riqueza específica y el 84,75 % en densidad de la fauna. Los resultados obtenidos en cuanto a la estructura de la población señalan que, para este tipo de estudios, son suficientes muestras cuya profundidad alcanza los 6 cm.Instituto Español de Oceanografí

Effect of trampling and digging from shell shing on Zostera noltei (Zosteraceae) intertidal seagrass beds

Seagrass beds are among the most valuable ecosystems in the world but they are also among the ones most affected by human activities, and they have decreased significantly in recent decades. In many areas, such as in the Basque Country (northern Spain), seagrass beds occupy areas that are also of interest for human activities such as recreation and shellfishing. They may therefore face a number of pressures that cause damage or irreversible states. Taking into account the limited distribution of seagrass beds in the Basque Country and the interest in their conservation, an eight-month field experiment focusing on the Zostera noltei growing season was carried out to evaluate the effect of shellfish gathering. We used generalized linear models to assess different intensities of trampling and digging, as the most important pressures of shellfishing applied to Zostera noltei beds. The results indicated that shoot density of Z. noltei was negatively altered by trampling treatments and positively affected (as a recovery) by digging treatments. This finding suggests that shellfishing adversely affects seagrass abundance and is potentially responsible for its low density in the Oka estuary. Our findings are important for management and should be taken into account in seagrass conservation and restoration programmes

Connectivity, neutral theories and the assessment of species vulnerability to global change in temperate estuaries

One of the main adaptation strategies to global change scenarios, aiming to preserve ecosystem functioning and biodiversity, is to maximise ecosystem resilience. The resilience of a species metapopulation can be improved by facilitating connectivity between local populations, which will prevent demographic stochasticity and inbreeding. The objective of this investigation is to estimate the degree of connectivity among estuarine species along the north-eastern Iberian coast, in order to assess community vulnerability to global change scenarios. To address this objective, two connectivity proxy types have been used based upon genetic and ecological drift processes: 1) DNA markers for the bivalve cockle (Cerastoderma edule) and seagrass Zostera noltei, and 2) the decrease in the number of species shared between two sites with geographic distance; neutral biodiversity theory predicts that dispersal limitation modulates this decrease, and this has been explored in estuarine plants and macroinvertebrates. Results indicate dispersal limitation for both saltmarsh plants and seagrass beds community and Z. noltei populations; this suggests they are especially vulnerable to expected climate changes on their habitats. In contrast, unstructured spatial pattern found in macroinvertebrate communities and in C. edule genetic populations in the area suggests that estuarine soft-bottom macroinvertebrates with planktonic larval dispersal strategies may have a high resilience capacity to moderate changes within their habitats. Our findings can help environmental managers to prioritise the most vulnerable species and habitats to be restored

Establishing a governance threshold in small-scale fisheries to achieve sustainability

The lack of effective governance is a major concern in small-scale fisheries. The implementation of governance that encompasses the three pillars of sustainability (social, economic, and ecological) is still a worldwide challenge. We examined nine stalked barnacle fisheries (Pollicipes pollicipes) across Southwest Europe to better understand the relationship between governance elements and sustainability. Our results show that nested spatial scales of management, the access structure, co- management, and fisher’s participation in monitoring and surveillance promote sustainability. However, it is not the mere presence of these elements but their level of implementation that drives sustainability. Efforts should be placed in the accomplishment of a minimum combination of local scales of management, access rights through individual quotas, instructive-consultative co- management and functional participation. Surpassing this threshold in future governance structures will start to adequately promote social, economic and ecologically sustainability in small-scale fisheries

Drug-loaded PCL electrospun nanofibers as anti-pancreatic cancer drug delivery systems

Cancer is one of the main causes of death worldwide, being pancreatic cancer the second deadliest cancer in Western countries. Surgery, chemotherapy and radiotherapy form the basis of pancreatic cancer's current treatment. However, these techniques have several disadvantages, such as surgery complications, chemotherapy systemic side effects and cancer recurrence. Drug delivery systems can reduce side effects, increasing the effectivity of the treatment by a controlled release at the targeted tumor cells. In this context, coaxial electrospun fibers can increase the control on the release profile of the drug. The aim of this study was to encapsulate and release different anticancer drugs (5-Fluorouracil and Methotrexate) from a polymeric fiber mat. Different flows and ratios were used to test their effect on fiber morphology, FTIR spectrum, drug encapsulation and release. Good integration of the anticancer drugs was observed and the use of a desiccator for 24 h showed to be a key step to remove solvent remanence. Moreover, the results of this study demonstrated that the polymeric solution could be used to encapsulate and release different drugs to treat cancers. This makes coaxial electrospinning a promising alternative to deliver complex chemotherapies that involve more than one drug, such as FOLFIRINOX, used in pancreatic cancer treatment

The Differential Impact of SRC Expression on the Prognosis of Patients with Head and Neck Squamous Cell Carcinoma

Aberrant SRC expression and activation is frequently detected in multiple cancers, and hence, targeting SRC has emerged as a promising therapeutic strategy. Different SRC inhibitors have demonstrated potent anti-tumor activity in preclinical models, although they largely lack clinical efficacy as monotherapy in late-stage solid tumors, including head and neck squamous cell carcinomas (HNSCC). Adequate selection and stratification of patients who may respond to and benefit from anti-SRC therapies is therefore needed to guide clinical trials and treatment efficacy. This study investigates the prognostic significance of active SRC expression in a homogeneous cohort of 122 human papillomavirus (HPV)-negative, surgically treated HNSCC patients. Immunohistochemical evaluation of the active form of SRC by means of anti-SRC Clone 28 monoclonal antibody was specifically performed and subsequently correlated with clinical data. The expression of p-SRC (Tyr419), total SRC, and downstream SRC effectors was also analyzed. Our results uncovered striking differences in the prognostic relevance of SRC expression in HNSCC patients depending on the tumor site. Active SRC expression was found to significantly associate with advanced disease stages, presence of lymph node metastasis, and tumor recurrences in patients with laryngeal tumors, but not in the pharyngeal subgroup. Multivariate Cox analysis further revealed active SRC expression as an independent predictor of cancer-specific mortality in patients with laryngeal carcinomas. Concordantly, expression of p-SRC (Tyr419) and the SRC substrates focal adhesion kinase (FAK) and the Arf GTPase-activating protein ASAP1 also showed specific associations with poor prognosis in the larynx. These findings could have important implications in ongoing Src family kinase (SFK)-based clinical trials, as these new criteria could help to improve patient selection and develop biomarker-stratified trials

Tele-rehabilitation versus local rehabilitation therapies assisted by robotic devices: a pilot study with patients

The present study aims to evaluate the advantages of a master-slave robotic rehabilitation therapy in which the patient is assisted in real-time by a therapist. We have also explored if this type of strategy is applicable in a tele-rehabilitation environment. A pilot study has been carried out involving 10 patients who have performed a point-to-point rehabilitation exercise supported by three assistance modalities: fixed assistance (without therapist interaction), local therapist assistance, and remote therapist assistance in a simulated tele-rehabiliation scenario. The rehabilitation exercise will be performed using an upper-limb rehabilitation robotic device that assists the patients through force fields. The results suggest that the assistance provided by the therapist is better adapted to patient needs than fixed assistance mode. Therefore, it maximizes the patient's level of effort, which is an important aspect to improve the rehabilitation outcomes. We have also seen that in a tele-rehabilitation environment it is more difficult to assess when to assist the patient than locally. However, the assistance suits patients better than the fixed assistance mode

YES1 drives lung cancer growth and progression and predicts sensitivity to dasatinib

Rationale: The characterization of new genetic alterations is essential to assign effective personalized therapies in non–small cell lung cancer (NSCLC). Furthermore, finding stratification biomarkers is essential for successful personalized therapies. Molecular alterations of YES1, a member of the SRC (proto-oncogene tyrosine-protein kinase Src) family kinases (SFKs), can be found in a significant subset of patients with lung cancer. Objectives: To evaluate YES1 (v-YES-1 Yamaguchi sarcoma viral oncogene homolog 1) genetic alteration as a therapeutic target and predictive biomarker of response to dasatinib in NSCLC. Methods: Functional significance was evaluated by in vivo models of NSCLC and metastasis and patient-derived xenografts. The efficacy of pharmacological and genetic (CRISPR [clustered regularly interspaced short palindromic repeats]/Cas9 [CRISPR-associated protein 9]) YES1 abrogation was also evaluated. In vitro functional assays for signaling, survival, and invasion were also performed. The association between YES1 alterations and prognosis was evaluated in clinical samples. Measurements and Main Results: We demonstrated that YES1 is essential for NSCLC carcinogenesis. Furthermore, YES1 overexpression induced metastatic spread in preclinical in vivo models. YES1 genetic depletion by CRISPR/Cas9 technology significantly reduced tumor growth and metastasis. YES1 effects were mainly driven by mTOR (mammalian target of rapamycin) signaling. Interestingly, cell lines and patient-derived xenograft models with YES1 gene amplifications presented a high sensitivity to dasatinib, an SFK inhibitor, pointing out YES1 status as a stratification biomarker for dasatinib response. Moreover, high YES1 protein expression was an independent predictor for poor prognosis in patients with lung cancer. Conclusions: YES1 is a promising therapeutic target in lung cancer. Our results provide support for the clinical evaluation of dasatinib treatment in a selected subset of patients using YES1 status as predictive biomarker for therapy