Amino acids content in 'Tempranillo' must from three soil types over four vintages

Amino acids are the main grape nitrogen compounds and the principal source of N for yeasts, being precursors of several volatile compounds. Therefore, N compound concentrations in musts can affect sensorial characteristics of wines. The aim of this study was to analyse the influence of N-NO3- and N-NH4+ contents from different soils on profile and content of amino acids in 'Tempranillo' grapes. In order to determine this soil influence on must quality, three plots were selected in AOC Rioja, classified as Fluventic Haploxerepts, Typic Calcixerepts, and Petrocalcic Palexerolls. The results showed that amino acids and yeast assimilable nitrogen (YAN) content allowed us to differentiate samples from the three soils, and in each soil type, samples of each season. In general, must contents of total amino acids and some of them, as alanine, threonine, and tyrosine, were more influenced by soil type; YAN, proline, histidine, serine, and glycine concentrations mainly depended on the interaction soil-vintage. In conclusion, free amino acids concentration could be a tool to differentiate musts coming from different soils

Towards the interoperability of computerised guidelines and electronic health records: an experiment with openEHR archetypes and a chronic heart failure guideline

Clinical guidelines contain recommendations based on the best empirical evidence available at the moment. There is a wide consensus about the benefits of guidelines and about the fact that they should be deployed through clinical information systems, making them available during clinical consultations. However, one of the main obstacles to this integration is the interaction with the electronic healthrecord system. With the aim of solving the interoperability problems of guideline systems, we have investigated the utilisation of the openEHR standardisation proposal in the context of one of the existing guideline representation languages. Concretely, we have designed a collection of archetypes to be used within a chronic heart failure guideline. The main contribution of our work is the utilisation of openEHR archetypes in the framework of guideline representation languages. Other contributions include both the concrete set of archetypes that we have selected and the methodological approach that we have followed to obtain itThis work has been supported by Fundaci´o Caixa Castell´o-Bancaixa, through the research project P11B2009-3

Changes on grape volatile composition after elicitors and nitrogen compounds foliar applications to 'Garnacha', 'Tempranillo' and 'Graciano' vines

Grape volatile compounds determine the wine quality and typicity. The aim of this work was to study the effects of several foliar applications in 'Garnacha', 'Tempranillo', and 'Graciano' grapevines in order to enhance their grape volatile composition. The field trial involved the application of two nitrogen compounds, urea (Ur) and phenylalanine (Phe), and two elicitors, methyl jasmonate (MeJ) and a yeast extract (YE). The analysis of grape volatile compounds was carried out by HS-SPME-GC-MS. For 'Garnacha', most terpenes, and C13 norisoprenoids increased their grape content by applying Ur and Phe, and especially MeJ. For 'Tempranillo', treatments with Ur and MeJ improved the synthesis of most terpenoids. For 'Graciano', a trend to decrease the terpenoids content in grapes with the treatments was observed; however, Phe application increased C13 norisoprenoids content. In conclusion, foliar application of, Phe or Ur, and an elicitor, MeJ, can be a useful tool to improve grape quality

Resighting of a Narwhal (Monodon monoceros) Instrumented with a Satellite Transmitter

On 9 August 2001, a male narwhal (Monodon monoceros) was instrumented with a satellite transmitter attached to the dorsal side of the tusk in Creswell Bay on Somerset Island in the Canadian High Arctic. The whale was identified five years later, on 22 June 2006, in a photo of a group of narwhals taken from the ice edge in Lancaster Sound. Examination of the position of the transmitter on the tusk showed that the tusk had grown approximately 17 cm in the five-year period. The transmitter was still located on the dorsal side of the tusk and this fact, together with the length of spiral grains at the position of the tag, indicates that the tusk had not turned over (rotated) in the five-year period. The whale was photographed on its spring migration back to the original summer ground where it had been tagged. This resighting confirms evidence for site fidelity of narwhals, as the individual maintained the same migratory schedule and route observed for narwhals from previous satellite tracking studies in Creswell Bay.Le 9 août 2001, un émetteur satellite a été posé sur un narval mâle (Monodon monoceros), plus précisément du côté dorsal de sa défense et ce, dans la baie Creswell, à l’île Somerset, dans l’Extrême-Arctique canadien. Cinq ans plus tard, soit le 22 juin 2006, la baleine a été aperçue dans la photo d’un groupe de narvals prise à partir de la lisière de glaces du détroit de Lancaster. L’examen de l’emplacement de l’émetteur sur la défense a permis de constater que la défense avait grandi d’environ 17 cm pendant la période de cinq ans. L’émetteur se trouvait toujours du côté dorsal de la défense et cela, allié à la longueur des fibres torses à l’emplacement de l’étiquette, indique que la défense n’avait pas subi de rotation pendant la période de cinq ans. La baleine a été photographiée pendant sa migration printanière de retour vers l’endroit où elle avait d’abord été étiquetée l’été. Ce repérage permet de confirmer les preuves relativement à la fidélité des narvals à leurs emplacements, car cette baleine avait maintenu les mêmes horaire et trajet migratoires observés chez les narvals à partir d’autres études de repérage par satellite réalisées dans la baie Creswell

Transcriptome Metabolic Characterization of Tuber borchii SP1—A New Spanish Strain for In Vitro Studies of the Bianchetto Truffle

Truffles are ascomycete hypogeous fungi belonging to the Tuberaceae family of the Pezizales order that grow in ectomycorrhizal symbiosis with tree roots, and they are known for their peculiar aromas and flavors. The axenic culture of truffle mycelium is problematic because it is not possible in many cases, and the growth rate is meager when it is possible. This limitation has prompted searching and characterizing new strains that can be handled in laboratory conditions for basic and applied studies. In this work, a new strain of Tuber borchii (strain SP1) was isolated and cultured, and its transcriptome was analyzed under different in vitro culture conditions. The results showed that the highest growth of T. borchii SP1 was obtained using maltose-enriched cultures made with soft-agar and in static submerged cultures made at 22 °C. We analyzed the transcriptome of this strain cultured in different media to establish a framework for future comparative studies, paying particular attention to the central metabolic pathways, principal secondary metabolite gene clusters, and the genes involved in producing volatile aromatic compounds (VOCs). The results showed a transcription signal for around 80% of the annotated genes. In contrast, most of the transcription effort was concentrated on a limited number of genes (20% of genes account for 80% of the transcription), and the transcription profile of the central metabolism genes was similar in the different conditions analyzed. The gene expression profile suggests that T. borchii uses fermentative rather than respiratory metabolism in these cultures, even in aerobic conditions. Finally, there was a reduced expression of genes belonging to secondary metabolite clusters, whereas there was a significative transcription of those involved in producing volatile aromatic compounds

A novel approach for adapting the standard addition method to single particle-ICP-MS for the accurate determination of NP size and number concentration in complex matrices; 35414390

This paper presents a novel approach, based on the standard addition method, for overcoming the matrix effects that often hamper the accurate characterization of nanoparticles (NPs) in complex samples via single particle inductively coupled plasma mass spectrometry (SP-ICP-MS). In this approach, calibration of the particle size is performed by two different methods: (i) by spiking a suspension of NPs standards of known size containing the analyte, or (ii) by spiking the sample with ionic standards; either way, the measured sensitivity is used in combination with the transport efficiency (TE) for sizing the NPs. Moreover, such transport efficiency can be readily obtained from the data obtained via both calibration methods mentioned above, so that the particle number concentration can also be determined. The addition of both ionic and NP standards can be performed on-line, by using a T-piece with two inlet lines of different dimensions. The smaller of the two is used for the standards, thus ensuring a constant and minimal sample dilution. As a result of the spiking of the samples, mixed histograms including the signal of the sample and that of the standards are obtained. However, the use of signal deconvolution approaches permits to extract the information, even in cases of signal populations overlapping. For proofing the concept, characterization of a 50 nm AuNPs suspension prepared in three different media (i.e., deionized water, 5% ethanol, and 2.5% tetramethyl ammonium hydroxide-TMAH) was carried out. Accurate results were obtained in all cases, in spite of the matrix effects detected in some media. Overall, the approach proposed offers flexibility, so it can be adapted to different situations, but it might be specially indicated for samples for which the matrix is not fully known and/or dilution is not possible/recommended. © 2022 The Author

Modeling healthcare authorization and claim submissions using the openEHR dual-model approach

<p>Abstract</p> <p>Background</p> <p>The TISS standard is a set of mandatory forms and electronic messages for healthcare authorization and claim submissions among healthcare plans and providers in Brazil. It is not based on formal models as the new generation of health informatics standards suggests. The objective of this paper is to model the TISS in terms of the openEHR archetype-based approach and integrate it into a patient-centered EHR architecture.</p> <p>Methods</p> <p>Three approaches were adopted to model TISS. In the first approach, a set of archetypes was designed using ENTRY subclasses. In the second one, a set of archetypes was designed using exclusively ADMIN_ENTRY and CLUSTERs as their root classes. In the third approach, the openEHR ADMIN_ENTRY is extended with classes designed for authorization and claim submissions, and an ISM_TRANSITION attribute is added to the COMPOSITION class. Another set of archetypes was designed based on this model. For all three approaches, templates were designed to represent the TISS forms.</p> <p>Results</p> <p>The archetypes based on the openEHR RM (Reference Model) can represent all TISS data structures. The extended model adds subclasses and an attribute to the COMPOSITION class to represent information on authorization and claim submissions. The archetypes based on all three approaches have similar structures, although rooted in different classes. The extended openEHR RM model is more semantically aligned with the concepts involved in a claim submission, but may disrupt interoperability with other systems and the current tools must be adapted to deal with it.</p> <p>Conclusions</p> <p>Modeling the TISS standard by means of the openEHR approach makes it aligned with ISO recommendations and provides a solid foundation on which the TISS can evolve. Although there are few administrative archetypes available, the openEHR RM is expressive enough to represent the TISS standard. This paper focuses on the TISS but its results may be extended to other billing processes. A complete communication architecture to simulate the exchange of TISS data between systems according to the openEHR approach still needs to be designed and implemented.</p