A new strategy for the cloning, overexpression and one step purification of three DHAP-dependent aldolases: rhamnulose-1-phosphate aldolase, fuculose-1-phosphate aldolase and tagatose-1,6-diphosphate aldolase

[[sponsorship]]基因體研究中心[[note]]已出版;[SCI];有審查制度;不具代表性[[note]]http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Drexel&SrcApp=hagerty_opac&KeyRecord=0968-0896&DestApp=JCR&RQ=IF_CAT_BOXPLOT[[note]]http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=RID&SrcApp=RID&DestLinkType=FullRecord&DestApp=ALL_WOS&KeyUT=A1995RK9750001

Cloning and overexpression of rhamnose isomerase and fucose isomerase

[[sponsorship]]基因體研究中心[[note]]已出版;[SCI];有審查制度;不具代表性[[note]]http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Drexel&SrcApp=hagerty_opac&KeyRecord=0968-0896&DestApp=JCR&RQ=IF_CAT_BOXPLOT[[note]]http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=RID&SrcApp=RID&DestLinkType=FullRecord&DestApp=ALL_WOS&KeyUT=A1995TB4940000

Synthèse chimioenzymatique d'aminocyclitols en tant qu'inhibiteurs de glycosidases

Communication oral

Synthèse chimioenzymatique d'aminocyclitols analogues de la valiolamine : vers le traitement de maladies lysosomales

Communication oral

Aldolases mediated synthesis of nitro- and aminocyclitols: towards treatment of lysosomal diseases

Poste

Bioinspired genotype–phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins

The idea of compartmentalization of genotype and phenotype in cells is key for enabling Darwinian evolution. This contribution describes bioinspired systems that use in vitro compartments—water-in-oil droplets and gel-shell beads—for the directed evolution of functional proteins. Technologies based on these principles promise to provide easier access to protein-based therapeutics, reagents for processes involving enzyme catalysis, parts for synthetic biology and materials with biological components

Microstructural and mechanical characterisation of ODS ferritic alloys produced by mechanical alloying and spark plasma sintering

Powders with nominal composition Fe-14Cr-2W-0·4Ti were mechanically alloyed (MA) with Y2O3 in a planetary ball mill at two different rotational speeds. Consolidation of the as milled powders was performed by spark plasma sintering (SPS). As milled powders showed a highly deformed microstructure with elongated nanometre grains and, depending upon the rotational speed, different stages of the nanocluster evolution were observed to be produced. In the case of consolidated materials, grain growth occurred during the SPS process and it was possible to observe the influence of the MA parameters, with larger and more homogeneously distributed grains at the higher rotational speed. Additionally, Ti was observed to be incorporated to the nanoclusters after SPS, indicating a further step in their evolution during consolidation. The mechanical behaviour of the SPS compacts was evaluated by tensile and small punch testing also showing the influence of the MA parameters in the material behaviour

Notch Impact Behavior of Oxide Dispersion Strengthened (ODS) Fe20Cr5Al alloy

In this paper tensile tests and LS and LT notched Charpy impact tests were performed at the temperature range between -196 and 200 °C on an oxide dispersion strengthened (ODS) Fe20Cr6Al0.5Y2O3 hot-rolled tube. The absorbed energy values in the range of high-temperatures of LS notched specimens is considerably higher than those of LT notched specimens; however such values tend to converge as temperature increases. Ductile fracture on the normal planes to RD with delaminations parallel to the tube surface were observed in the temperature range between RT and 200 °C. Delaminations of crack divider type were observed in LT specimens, whereas delaminations of crack arrester type were observed in LS specimens. The yttria particles in the grain boundaries and the transverse plastic anisotropy are the possible causes of that the delaminations were parallel to the tube surfacePM 2000 is a trademark of Plansee GmbH. The authors acknowledge the financial support of the Spanish Ministerio de Economia e Innovacio´ n (MINECO) in the form of a Coordinate Project in the Energy Area of Plan Nacional 2009 (ENE2009-13766-C04-01). G.P. acknowledges MINECO for financial support in the form of PhD Research Grant (FPI). This research was supported by ORNL’s Shared Research Equipment (SHaRE) User Facility, which is sponsored by the Office of Basic Energy Sciences, U.S. Department of EnergyPeer reviewe